Harald Mischak

Summary

Country: UK

Publications

  1. ncbi request reprint How to get proteomics to the clinic? Issues in clinical proteomics, exemplified by CE-MS
    Harald Mischak
    BHF Glasgow Cardiovascular Research Centre, Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK
    Proteomics Clin Appl 6:437-42. 2012
  2. pmc Implementation of proteomic biomarkers: making it work
    Harald Mischak
    BHF Glasgow Cardiovascular Research Centre, Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK
    Eur J Clin Invest 42:1027-36. 2012
  3. ncbi request reprint Recent advances in capillary electrophoresis coupled to mass spectrometry for clinical proteomic applications
    Angelique Stalmach
    Department of Proteomics and Systems Medicine, BHF Glasgow Cardiovascular Research Centre, Institute of Cardiovascular and Medical Sciences, College of Medical Veterinary and Life Sciences, University of Glasgow, Glasgow, UK
    Electrophoresis 34:1452-64. 2013
  4. ncbi request reprint Improving peptide relative quantification in MALDI-TOF MS for biomarker assessment
    Amaya Albalat
    Institute of Cardiovascular and Medical Sciences, University of Glasgow, Glasgow, Scotland, UK
    Proteomics 13:2967-75. 2013
  5. ncbi request reprint Urinary proteomics based on capillary electrophoresis coupled to mass spectrometry in kidney disease
    Amaya Albalat
    BHF Glasgow Cardiovascular Research Centre, University of Glasgow, Glasgow, UK
    Methods Mol Biol 919:203-13. 2013
  6. ncbi request reprint Performance of different separation methods interfaced in the same MS-reflection TOF detector: a comparison of performance between CE versus HPLC for biomarker analysis
    William Mullen
    School of Life Sciences, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK
    Electrophoresis 33:567-74. 2012
  7. ncbi request reprint Proteome-based systems biology analysis of the diabetic mouse aorta reveals major changes in fatty acid biosynthesis as potential hallmark in diabetes mellitus-associated vascular disease
    Holger Husi
    Institute of Cardiovascular and Medical Sciences, University of Glasgow, BHF Glasgow Cardiovascular Research Centre, Glasgow, UK
    Circ Cardiovasc Genet 7:161-70. 2014
  8. ncbi request reprint A peptidomic approach to biomarker discovery for bovine mastitis
    Rozaihan Mansor
    Institute of Infection, Immunity and Inflammation, University of Glasgow, Glasgow, UK
    J Proteomics 85:89-98. 2013
  9. pmc Urinary proteomics to support diagnosis of stroke
    Jesse Dawson
    Institute of Cardiovascular and Medical Sciences, College of Medicine, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom
    PLoS ONE 7:e35879. 2012
  10. ncbi request reprint Methods in capillary electrophoresis coupled to mass spectrometry for the identification of clinical proteomic/peptidomic biomarkers in biofluids
    Angelique Stalmach
    BHF Glasgow Cardiovascular Research Centre, Institute of Cardiovascular and Medical Sciences, College of Medical Veterinary and Life Sciences, University of Glasgow, Glasgow, UK
    Methods Mol Biol 1243:187-205. 2015

Collaborators

Detail Information

Publications19

  1. ncbi request reprint How to get proteomics to the clinic? Issues in clinical proteomics, exemplified by CE-MS
    Harald Mischak
    BHF Glasgow Cardiovascular Research Centre, Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK
    Proteomics Clin Appl 6:437-42. 2012
    ..In this article, some key issues in proteomic biomarker research are pinpointed, based on the experience with CE-MS, likely also holding true for biomarkers resulting from other analysis domains...
  2. pmc Implementation of proteomic biomarkers: making it work
    Harald Mischak
    BHF Glasgow Cardiovascular Research Centre, Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK
    Eur J Clin Invest 42:1027-36. 2012
    ..This approach may avoid unwarranted delays or failure to implement potentially useful biomarkers, and may expedite meaningful contributions of the biomarker community to healthcare...
  3. ncbi request reprint Recent advances in capillary electrophoresis coupled to mass spectrometry for clinical proteomic applications
    Angelique Stalmach
    Department of Proteomics and Systems Medicine, BHF Glasgow Cardiovascular Research Centre, Institute of Cardiovascular and Medical Sciences, College of Medical Veterinary and Life Sciences, University of Glasgow, Glasgow, UK
    Electrophoresis 34:1452-64. 2013
    ..In the end, we provide a short outlook, and indicate future developments to be expected, as well as problems that need to be solved to enable routine application of CE-MS in a clinical setting...
  4. ncbi request reprint Improving peptide relative quantification in MALDI-TOF MS for biomarker assessment
    Amaya Albalat
    Institute of Cardiovascular and Medical Sciences, University of Glasgow, Glasgow, Scotland, UK
    Proteomics 13:2967-75. 2013
    ..This concept was successfully applied to a set of urine samples from patients diagnosed with diabetes presenting normoalbuminuria (controls) and macroalbuminuria (cases)...
  5. ncbi request reprint Urinary proteomics based on capillary electrophoresis coupled to mass spectrometry in kidney disease
    Amaya Albalat
    BHF Glasgow Cardiovascular Research Centre, University of Glasgow, Glasgow, UK
    Methods Mol Biol 919:203-13. 2013
    ..This technology has proven to be highly reproducible, sensitive with a quick analysis time, important features when analytical platforms have to be used in a clinical setting...
  6. ncbi request reprint Performance of different separation methods interfaced in the same MS-reflection TOF detector: a comparison of performance between CE versus HPLC for biomarker analysis
    William Mullen
    School of Life Sciences, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK
    Electrophoresis 33:567-74. 2012
    ..In addition, CE was shown not to suffer from carry over from previous samples as was seen in the LC analysis...
  7. ncbi request reprint Proteome-based systems biology analysis of the diabetic mouse aorta reveals major changes in fatty acid biosynthesis as potential hallmark in diabetes mellitus-associated vascular disease
    Holger Husi
    Institute of Cardiovascular and Medical Sciences, University of Glasgow, BHF Glasgow Cardiovascular Research Centre, Glasgow, UK
    Circ Cardiovasc Genet 7:161-70. 2014
    ..However, better understanding of systemic effects is essential in unraveling key molecular events in the vascular tissue responsible for disease onset and progression...
  8. ncbi request reprint A peptidomic approach to biomarker discovery for bovine mastitis
    Rozaihan Mansor
    Institute of Infection, Immunity and Inflammation, University of Glasgow, Glasgow, UK
    J Proteomics 85:89-98. 2013
    ..casein fragments were the most common of the peptides in this model. Peptide biomarkers of milk could be used in the diagnosis of mastitis and can discriminate between these two bacterial causes...
  9. pmc Urinary proteomics to support diagnosis of stroke
    Jesse Dawson
    Institute of Cardiovascular and Medical Sciences, College of Medicine, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom
    PLoS ONE 7:e35879. 2012
    ..We now plan to refine further and explore the clinical utility of such a test in large prospective clinical trials...
  10. ncbi request reprint Methods in capillary electrophoresis coupled to mass spectrometry for the identification of clinical proteomic/peptidomic biomarkers in biofluids
    Angelique Stalmach
    BHF Glasgow Cardiovascular Research Centre, Institute of Cardiovascular and Medical Sciences, College of Medical Veterinary and Life Sciences, University of Glasgow, Glasgow, UK
    Methods Mol Biol 1243:187-205. 2015
    ..In this chapter, we report on the materials, methods and protocols used for CE-MS-based clinical proteomics allowing the reproducible profiling of biofluids. ..
  11. pmc A combinatorial approach of Proteomics and Systems Biology in unravelling the mechanisms of acute kidney injury (AKI): involvement of NMDA receptor GRIN1 in murine AKI
    Holger Husi
    BHF Glasgow Cardiovascular Research Centre, University of Glasgow, 126 University Place, Glasgow G12 8TA, UK
    BMC Syst Biol 7:110. 2013
    ....
  12. ncbi request reprint Urinary proteomics in the assessment of chronic kidney disease
    William Mullen
    College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK
    Curr Opin Nephrol Hypertens 20:654-61. 2011
    ..We review several of the recent publications reporting on larger cohorts, focusing on those that aim at qualification and/or validation of urinary proteomics biomarkers...
  13. ncbi request reprint Identification of urinary peptide biomarkers associated with rheumatoid arthritis
    Angelique Stalmach
    University of Glasgow, Institute of Cardiovascular and Medical Sciences, Glasgow, United Kingdom
    PLoS ONE 9:e104625. 2014
    ..Further studies are currently being undertaken in larger cohorts of patients with rheumatoid arthritis and other athridities to assess the potential of the urinary peptide based classifier in the early detection of rheumatoid arthritis. ..
  14. ncbi request reprint Technical aspects and inter-laboratory variability in native peptide profiling: the CE-MS experience
    Harald Mischak
    BHF Glasgow Cardiovascular Research Centre, University of Glasgow, G12 8TA Glasgow, United Kingdom
    Clin Biochem 46:432-43. 2013
    ..Finally, we discuss pros and cons and means for improvement and emphasize the need to test in terms of comparative clinical performance and impact, different platforms that pass reasonably well analytical validation tests...
  15. ncbi request reprint Clinical application of urinary proteomics/peptidomics
    Amaya Albalat
    BHF Glasgow Cardiovascular Research Centre, Institute of Cardiovascular and Medical Sciences, College of Medical Veterinary and Life Sciences, University of Glasgow, UK
    Expert Rev Proteomics 8:615-29. 2011
    ..Finally, current challenges and considerations for future studies will be discussed...
  16. ncbi request reprint Urinary proteomic diagnosis of coronary artery disease: identification and clinical validation in 623 individuals
    Christian Delles
    Faculty of Medicine, University of Glasgow, Glasgow, UK
    J Hypertens 28:2316-22. 2010
    ..We studied the urinary proteome in a total of 623 individuals with and without coronary artery disease (CAD) in order to characterize multiple biomarkers that enable prediction of the presence of CAD...
  17. ncbi request reprint Impact of a 6-wk olive oil supplementation in healthy adults on urinary proteomic biomarkers of coronary artery disease, chronic kidney disease, and diabetes (types 1 and 2): a randomized, parallel, controlled, double-blind study
    Sandra Silva
    From the Analytical Services Unit, Instituto de Biologia Experimental Tecnologica, Oeiras, Portugal SS and MRB the Analytical Chemistry Department, Instituto de Tecnologia Química e Biológica, Oeiras, Portugal SS and MRB the Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal SS and MRB the Research Institute for Medicines and Pharmaceutical Sciences, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal MRB and MEF Mosaiques Diagnostics AG, Hannover, Germany JS and HM and Human Nutrition, School of Medicine EC and the Institute of Cardiovascular and Medical Sciences WM, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom
    Am J Clin Nutr 101:44-54. 2015
    ..Proteomic biomarkers enable an early, presymptomatic diagnosis of disease, which makes them important and effective, but understudied, tools for primary prevention...
  18. ncbi request reprint Proteomics and metabolomics as tools to unravel novel culprits and mechanisms of uremic toxicity: instrument or hype?
    William Mullen
    British Heart Foundation Glasgow Cardiovascular Research Centre, University of Glasgow, Glasgow, United Kingdom Electronic address
    Semin Nephrol 34:180-90. 2014
    ..We highlight the most promising findings, indicate where we see the current need, and which future developments consequently are to be expected, given the technological constraints that undoubtedly exist. ..
  19. ncbi request reprint Urinary proteomics for prediction of preeclampsia
    David M Carty
    Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary, and Life Sciences, University of Glasgow, Glasgow, UK
    Hypertension 57:561-9. 2011
    ..After prospective validation in other cohorts, these markers may contribute to better prediction, monitoring, and accurate diagnosis of preeclampsia...