M A Miles

Summary

Affiliation: London School of Hygiene and Tropical Medicine
Country: UK

Publications

  1. pmc North American import? Charting the origins of an enigmatic Trypanosoma cruzi domestic genotype
    Federico A Zumaya-Estrada
    Centro Regional de Investigación en Salud Pública, Instituto Nacional de Salud Publica, Tapachula, Chiapas, Mexico
    Parasit Vectors 5:226. 2012
  2. pmc Persistent digestive disorders in the tropics: causative infectious pathogens and reference diagnostic tests
    Sören L Becker
    Department of Epidemiology and Public Health, Swiss Tropical and Public Health Institute, Basel, Switzerland
    BMC Infect Dis 13:37. 2013
  3. pmc Comparative genomic analysis of human infective Trypanosoma cruzi lineages with the bat-restricted subspecies T. cruzi marinkellei
    Oscar Franzén
    Department of Cell and Molecular Biology, Karolinska Institutet, Box 285, Stockholm SE 17177, Sweden
    BMC Genomics 13:531. 2012
  4. ncbi request reprint The discovery of Chagas disease: progress and prejudice
    Michael A Miles
    Pathogen Molecular Biology Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK
    Infect Dis Clin North Am 18:247-60, table of contents. 2004
  5. doi request reprint The molecular epidemiology and phylogeography of Trypanosoma cruzi and parallel research on Leishmania: looking back and to the future
    M A Miles
    Pathogen Molecular Biology Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London, UK
    Parasitology 136:1509-28. 2009
  6. pmc American trypanosomiasis (Chagas' disease) and the role of molecular epidemiology in guiding control strategies
    Michael A Miles
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT
    BMJ 326:1444-8. 2003
  7. ncbi request reprint Temperature gradient gel electrophoresis (TGGE) analysis of riboprints from Trypanosoma cruzi
    J R Stothard
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine
    Parasitology 117:249-53. 1998
  8. ncbi request reprint On the molecular taxonomy of Trypanosoma cruzi using riboprinting
    J R Stothard
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine
    Parasitology 117:243-7. 1998
  9. ncbi request reprint Genetic typing and phylogeny of the Leishmania donovani complex by restriction analysis of PCR amplified gp63 intergenic regions
    I L Mauricio
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, UK
    Parasitology 122:393-403. 2001
  10. ncbi request reprint Biogeography of Triatominae (Hemiptera: Reduviidae) in Ecuador: implications for the design of control strategies
    F Abad-Franch
    Pathogen Molecular Biology and Biochemistry Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, UK
    Mem Inst Oswaldo Cruz 96:611-20. 2001

Collaborators

Detail Information

Publications37

  1. pmc North American import? Charting the origins of an enigmatic Trypanosoma cruzi domestic genotype
    Federico A Zumaya-Estrada
    Centro Regional de Investigación en Salud Pública, Instituto Nacional de Salud Publica, Tapachula, Chiapas, Mexico
    Parasit Vectors 5:226. 2012
    ..To provide further insight we undertook high resolution nuclear and mitochondrial genotyping of multiple Central American strains (from areas of México and Guatemala) and included them in an analysis with other published data...
  2. pmc Persistent digestive disorders in the tropics: causative infectious pathogens and reference diagnostic tests
    Sören L Becker
    Department of Epidemiology and Public Health, Swiss Tropical and Public Health Institute, Basel, Switzerland
    BMC Infect Dis 13:37. 2013
    ..We provide an overview of pathogens causing digestive disorders in the tropics and evaluate available reference tests...
  3. pmc Comparative genomic analysis of human infective Trypanosoma cruzi lineages with the bat-restricted subspecies T. cruzi marinkellei
    Oscar Franzén
    Department of Cell and Molecular Biology, Karolinska Institutet, Box 285, Stockholm SE 17177, Sweden
    BMC Genomics 13:531. 2012
    ..cruzi subspecies. Here we report a draft genome sequence of T. c. marinkellei and comparison with T. c. cruzi. Our aims were to identify unique sequences and genomic features, which may relate to their distinct niches...
  4. ncbi request reprint The discovery of Chagas disease: progress and prejudice
    Michael A Miles
    Pathogen Molecular Biology Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK
    Infect Dis Clin North Am 18:247-60, table of contents. 2004
    ..In addition, there is a background story line of controversy, jealousy, and power politics. This article provides a synopsis of all these fascinating aspects of the discovery...
  5. doi request reprint The molecular epidemiology and phylogeography of Trypanosoma cruzi and parallel research on Leishmania: looking back and to the future
    M A Miles
    Pathogen Molecular Biology Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London, UK
    Parasitology 136:1509-28. 2009
    ..We describe the profound impact of this parallel research on genetic diversity of T. cruzi and Leishmania, in the context of epidemiology, taxonomy and disease control...
  6. pmc American trypanosomiasis (Chagas' disease) and the role of molecular epidemiology in guiding control strategies
    Michael A Miles
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT
    BMJ 326:1444-8. 2003
  7. ncbi request reprint Temperature gradient gel electrophoresis (TGGE) analysis of riboprints from Trypanosoma cruzi
    J R Stothard
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine
    Parasitology 117:249-53. 1998
    ..This seriously questions the current molecular phylogeny of these protozoa using 18S data...
  8. ncbi request reprint On the molecular taxonomy of Trypanosoma cruzi using riboprinting
    J R Stothard
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine
    Parasitology 117:243-7. 1998
    ..It would therefore appear premature to warrant division of T. cruzi into 2 discrete taxa or subspecies until the relationships of further zymodeme 3 stocks are elucidated...
  9. ncbi request reprint Genetic typing and phylogeny of the Leishmania donovani complex by restriction analysis of PCR amplified gp63 intergenic regions
    I L Mauricio
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, UK
    Parasitology 122:393-403. 2001
    ..infantum. No evidence was found to support the existence of L. archibaldi. It is likely to be necessary in future to re-evaluate the taxonomic status of L. donovani or of L. infantum, as discrete species...
  10. ncbi request reprint Biogeography of Triatominae (Hemiptera: Reduviidae) in Ecuador: implications for the design of control strategies
    F Abad-Franch
    Pathogen Molecular Biology and Biochemistry Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, UK
    Mem Inst Oswaldo Cruz 96:611-20. 2001
    ..Local eradication of T. dimidiata and these R. ecuadoriensis populations might therefore be attainable. However, the presence of a wide variety of native species indicates the necessity for a strong longitudinal surveillance system...
  11. ncbi request reprint Genomic diversity in the Leishmania donovani complex
    I L Mauricio
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, UK
    Parasitology 119:237-46. 1999
    ..chagasi was identified as a monophyletic group within the L. donovani complex but L. donovani may be paraphyletic. Diversity within L. donovani is substantial and phylogeographical patterns of association were found...
  12. ncbi request reprint Analysis of genetic diversity of Trypanosoma cruzi: an application of riboprinting and gradient gel electrophoresis methods
    J R Stothard
    Pathogen Molecular Biology and Biochemistry Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, UK
    Mem Inst Oswaldo Cruz 95:545-51. 2000
    ..These divergent 18S paralogues may have, or had, different functional pressures or rates of molecular evolution. Whether or not these divergent types are equally transcriptionally active throughout the life cycle, remain to be assessed...
  13. pmc A panel of ten microsatellite loci for the Chagas disease vector Rhodnius prolixus (Hemiptera: Reduviidae)
    S Fitzpatrick
    Pathogen and Molecular Biology Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E7HT, UK
    Infect Genet Evol 9:206-9. 2009
    ..32 to 0.66. The microsatellite markers presented here will contribute to the control of Chagas disease in Venezuela and Colombia through the provision of population information that may allow the design of improved control strategies...
  14. ncbi request reprint Use of polymerase chain reaction-based single strand conformational polymorphism and denaturing gradient gel electrophoresis methods for detection of sequence variation of ribosomal DNA of Trypanosoma cruzi
    J R Stothard
    Department of Medical Parasitology, London School of Hygiene and Tropical Medicine, U K
    Int J Parasitol 27:339-43. 1997
    ..where only 1 was identified. This is the first application of PCR-linked SSCP and DGGE analysis for differentiating parasitic protozoa...
  15. ncbi request reprint Multiple hybrid genotypes of Leishmania (viannia) in a focus of mucocutaneous Leishmaniasis
    Debbie Nolder
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom
    Am J Trop Med Hyg 76:573-8. 2007
    ..Thus hybrids must be included among the potential agents of MCL. Despite the propensity for clonality, hybrids are also an important feature of Leishmania (Viannia) and may give rise to epidemiologically important emergent genotypes...
  16. ncbi request reprint An insect molecular clock dates the origin of the insects and accords with palaeontological and biogeographic landmarks
    Michael W Gaunt
    Pathogen Molecular Biology and Biochemistry Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
    Mol Biol Evol 19:748-61. 2002
    ..The major output of the study is a comprehensive series of dates for deep-branching points within insect evolution that can act as calibration points for further dating studies within insect families and genera...
  17. ncbi request reprint Glycoprotein 63 (gp63) genes show gene conversion and reveal the evolution of Old World Leishmania
    Isabel L Mauricio
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
    Int J Parasitol 37:565-76. 2007
    ..donovani complex. Our results confirmed that a more stringent definition of Leishmania infantum is required and that the species Leishmania archibaldi should be suppressed...
  18. ncbi request reprint Resolution of multiclonal infections of Trypanosoma cruzi from naturally infected triatomine bugs and from experimentally infected mice by direct plating on a sensitive solid medium
    Matthew Yeo
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT, UK
    Int J Parasitol 37:111-20. 2007
    ..Furthermore, the method is effective for the isolation of clones directly from silvatic triatomine bugs and from experimentally infected mice harbouring mixed infections, allowing resolution of multiclonal infections from varied sources...
  19. pmc Flow cytometric analysis and microsatellite genotyping reveal extensive DNA content variation in Trypanosoma cruzi populations and expose contrasts between natural and experimental hybrids
    Michael D Lewis
    Pathogen Molecular Biology Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT, UK
    Int J Parasitol 39:1305-17. 2009
    ..The results are discussed in the context of hybridisation mechanisms in both natural and in vitro settings...
  20. ncbi request reprint Towards multilocus sequence typing of the Leishmania donovani complex: resolving genotypes and haplotypes for five polymorphic metabolic enzymes (ASAT, GPI, NH1, NH2, PGD)
    Isabel L Mauricio
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
    Int J Parasitol 36:757-69. 2006
    ..Furthermore, it can be used to detect recombination indirectly and for population genetics studies...
  21. pmc Molecular genetics reveal that silvatic Rhodnius prolixus do colonise rural houses
    Sinead Fitzpatrick
    Pathogen Molecular Biology Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom
    PLoS Negl Trop Dis 2:e210. 2008
    ..Here we investigate, by direct sequencing (mtcytb, D2) and by microsatellite analysis, 1) the identity of silvatic Rhodnius and 2) whether silvatic populations of Rhodnius are isolated from domestic populations...
  22. ncbi request reprint Origins of Chagas disease: Didelphis species are natural hosts of Trypanosoma cruzi I and armadillos hosts of Trypanosoma cruzi II, including hybrids
    Matthew Yeo
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London, UK
    Int J Parasitol 35:225-33. 2005
    ..cruzi intraspecific diversity in South America, and for separate origins of Chagas disease in northern South America and in the southern cone countries...
  23. pmc Genome-scale multilocus microsatellite typing of Trypanosoma cruzi discrete typing unit I reveals phylogeographic structure and specific genotypes linked to human infection
    Martin S Llewellyn
    London School of Hygiene and Tropical Medicine, London, UK
    PLoS Pathog 5:e1000410. 2009
    ..The epidemiological significance of these findings is discussed...
  24. ncbi request reprint The Trypanosoma cruzi enzyme TcGPXI is a glycosomal peroxidase and can be linked to trypanothione reduction by glutathione or tryparedoxin
    Shane R Wilkinson
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, United Kingdom
    J Biol Chem 277:17062-71. 2002
    ..The ability of TcGPXI to use alternative electron donors may reflect their availability at the corresponding subcellular sites...
  25. pmc Genotyping of Trypanosoma cruzi: systematic selection of assays allowing rapid and accurate discrimination of all known lineages
    Michael D Lewis
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom
    Am J Trop Med Hyg 81:1041-9. 2009
    ..Based upon the results we recommend a triple-assay comprising the LSU rDNA, HSP60 and GPI markers for reliable, rapid, low-cost DTU assignment...
  26. doi request reprint Analysis of molecular diversity of the Trypanosoma cruzi trypomastigote small surface antigen reveals novel epitopes, evidence of positive selection and potential implications for lineage-specific serology
    Tapan Bhattacharyya
    Pathogen Molecular Biology Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E7HT, UK
    Int J Parasitol 40:921-8. 2010
    ..233) indicates that the TSSA gene has been under positive selection pressure. The demonstration of lineage-specific epitopes within TcII-VI has implications for sero-epidemiological studies of Chagas disease based on this antigen...
  27. ncbi request reprint Field ecology of sylvatic Rhodnius populations (Heteroptera, Triatominae): risk factors for palm tree infestation in western Ecuador
    F Abad-Franch
    Department of Infectious and Tropical Diseases, Pathogen Molecular Biology Unit, London School of Hygiene and Tropical Medicine, London, UK
    Trop Med Int Health 10:1258-66. 2005
    ..An outline of 'risk palm ecotopes' and environmental management-based strategies for the control of peridomestic, palm tree-living vector populations are proposed...
  28. ncbi request reprint Mechanism of genetic exchange in American trypanosomes
    Michael W Gaunt
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
    Nature 421:936-9. 2003
    ..cruzi. In this instance, aneuploidy through nuclear hybridization results in recombination across far greater genetic distances than mendelian genetic exchange. This mechanism also parallels genome duplication...
  29. ncbi request reprint Leishmania donovani complex: genotyping with the ribosomal internal transcribed spacer and the mini-exon
    I L Mauricio
    Pathogen Molecular Biology Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
    Parasitology 128:263-7. 2004
    ..donovani complex strains and Old World Leishmania species. The targets produced less robust groups than gp63 intergenic regions, but support the need for re-evaluation of the taxonomy of the L. donovani complex...
  30. ncbi request reprint Population morphometric analysis of the tropicopolitan bug Triatoma rubrofasciata and relationships with old world species of Triatoma: evidence of New World ancestry
    J S Patterson
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, University of London, UK
    Med Vet Entomol 15:443-51. 2001
    ..We interpret this to imply a common ancestry for these groups. Dissemination of T. rubrofasciata and subsequent derivation of the Old World species of Triatoma is deduced to have occurred over a period of not more than 350 years...
  31. pmc The anti-influenza virus drug rimantadine has trypanocidal activity
    J M Kelly
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, United Kingdom
    Antimicrob Agents Chemother 43:985-7. 1999
    ..4). The activity is pH dependent and is consistent with a mechanism involving inhibition of the ability to regulate internal pH. Rimantadine is also toxic to the trypanosomatid parasites Trypanosoma cruzi and Leishmania major...
  32. ncbi request reprint Genetic polymorphism within the leishmania donovani complex: correlation with geographic origin
    Eva Zemanova
    Institute of Parasitology, Czech Academy of Sciences, and Faculty of Biology, University of South Bohemia, Ceske Budejovice, Czech Republic
    Am J Trop Med Hyg 70:613-7. 2004
    ..infantum was paraphyletic. The RAPD profiles suggest that MON-30 belongs to L. donovani. Moreover, the genetic distance between the L. archibaldi strain and other leishmanias does not warrant existence of a separate species...
  33. ncbi request reprint The triatomines of northern Peru, with emphasis on the ecology and infection by trypanosomes of Rhodnius ecuadoriensis (Triatominae)
    Cesar A Cuba Cuba
    Unidade de Parasitologia Médica Patologia, Faculdade de Medicina, Universidade de Brasilia, Brasilia, DF, Brasil
    Mem Inst Oswaldo Cruz 97:175-83. 2002
    ..A strong community-based entomological surveillance system and collaboration with Ecuadorian public health authorities and researchers are recommended...
  34. ncbi request reprint Emerging Chagas disease in Amazonian Brazil
    José Rodrigues Coura
    Dept of Tropical Medicine, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil
    Trends Parasitol 18:171-6. 2002
    ..cruziZ3/Z1 ASAT--are present. It appears that T. cruzil has an extant capacity for genetic exchange. Attention is also drawn to the risk of domestic endemicity, in addition to the tasks facing the disease control authorities...
  35. ncbi request reprint The Leishmania donovani complex: genotypes of five metabolic enzymes (ICD, ME, MPI, G6PDH, and FH), new targets for multilocus sequence typing
    Eva Zemanova
    Biology Centre, Institute of Parasitology, Czech Academy of Sciences and Faculty of Biology, University of South Bohemia, Ceske Budejovice, Budweis, Czech Republic
    Int J Parasitol 37:149-60. 2007
    ..Phylogenetic analysis of a concatenated alignment based on a total of 263 kb protein-coding sequences showed strong correlation of genotype with geographical origin. Europe and Africa appear to represent independent evolutionary centres...
  36. ncbi request reprint Malaria--there could be a third way
    Michael A Miles
    Nature 421:13. 2003
  37. pmc Evolutionary and geographical history of the Leishmania donovani complex with a revision of current taxonomy
    Julius Lukes
    Biology Centre, Institute of Parasitology, Czech Academy of Sciences, and Faculty of Biology, University of South Bohemia, 370 05 Ceske Budejovice, Czech Republic
    Proc Natl Acad Sci U S A 104:9375-80. 2007
    ..4 and 0.8 Mya. The prevailing mode of reproduction is clonal, but there is evidence of genetic exchange between strains, particularly in Africa...