Klaus Suhling

Summary

Affiliation: King's College London
Country: UK

Publications

  1. ncbi request reprint Time-resolved fluorescence microscopy
    Klaus Suhling
    Department of Physics, King s College London, Strand, London, UK
    Photochem Photobiol Sci 4:13-22. 2005
  2. ncbi request reprint Time-resolved fluorescence anisotropy imaging
    Klaus Suhling
    Department of Physics, King s College London, London, UK
    Methods Mol Biol 1076:503-19. 2014
  3. doi request reprint Fluorescence lifetime and polarization-resolved imaging in cell biology
    James A Levitt
    Department of Physics, King s College London, London, UK
    Curr Opin Biotechnol 20:28-36. 2009
  4. doi request reprint A targeted siRNA screen identifies regulators of Cdc42 activity at the natural killer cell immunological synapse
    Leo M Carlin
    Richard Dimbleby Department of Cancer Research, King s College London, London SE1 1UL, UK
    Sci Signal 4:ra81. 2011
  5. doi request reprint Fluorescence anisotropy of molecular rotors
    James A Levitt
    Department of Physics, King s College London, Strand, London, WC2R 2LS, UK
    Chemphyschem 12:662-72. 2011
  6. ncbi request reprint Gd-containing conjugated polymer nanoparticles: bimodal nanoparticles for fluorescence and MRI imaging
    Zeina Hashim
    Department of Physics, King s College London, Strand, London, WC2R 2LS, UK
    Nanoscale 6:8376-86. 2014
  7. ncbi request reprint Influence of molecular shape, conformability, net surface charge, and tissue interaction on transscleral macromolecular diffusion
    Nishanthan Srikantha
    Department of Physics, King s College London, United Kingdom School of Medicine, King s College London, London, United Kingdom
    Exp Eye Res 102:85-92. 2012
  8. ncbi request reprint Imaging immune surveillance by T cells and NK cells
    Fiona E McCann
    Department of Biological Sciences, Sir Alexander Fleming Building, Imperial College of Science, Technology and Medicine, London, UK
    Immunol Rev 189:179-92. 2002
  9. doi request reprint Rapid wide-field photon counting imaging with microsecond time resolution
    Klaus Suhling
    Department of Physics, King s College London, Strand, London WC2R 2LS, UK
    Opt Express 18:25292-8. 2010
  10. doi request reprint Direct binding of Grb2 SH3 domain to FGFR2 regulates SHP2 function
    Zamal Ahmed
    Department of Structural and Molecular Biology, University College London, Gower Street, London, WC1E 6BT, UK
    Cell Signal 22:23-33. 2010

Collaborators

Detail Information

Publications20

  1. ncbi request reprint Time-resolved fluorescence microscopy
    Klaus Suhling
    Department of Physics, King s College London, Strand, London, UK
    Photochem Photobiol Sci 4:13-22. 2005
    ..In addition, FLIM can also probe the local environment of fluorophores, reporting, for example, on the local pH, refractive index, ion or oxygen concentration without the need for ratiometric measurements...
  2. ncbi request reprint Time-resolved fluorescence anisotropy imaging
    Klaus Suhling
    Department of Physics, King s College London, London, UK
    Methods Mol Biol 1076:503-19. 2014
    ..Polarization-resolved fluorescence lifetime imaging-time-resolved fluorescence anisotropy imaging, TR-FAIM-allows mapping of viscosity or binding or of homo-FRET which can indicate dimerization or generally oligomerization. ..
  3. doi request reprint Fluorescence lifetime and polarization-resolved imaging in cell biology
    James A Levitt
    Department of Physics, King s College London, London, UK
    Curr Opin Biotechnol 20:28-36. 2009
    ..The particular merits of polarization-resolved fluorescence measurements are highlighted, including the unique ability to elucidate the occurrence of homo-FRET...
  4. doi request reprint A targeted siRNA screen identifies regulators of Cdc42 activity at the natural killer cell immunological synapse
    Leo M Carlin
    Richard Dimbleby Department of Cancer Research, King s College London, London SE1 1UL, UK
    Sci Signal 4:ra81. 2011
    ..Given that PI3Ks are targets of tumor therapies, our findings suggest the need to monitor innate immune function during the course of targeted therapy against these enzymes...
  5. doi request reprint Fluorescence anisotropy of molecular rotors
    James A Levitt
    Department of Physics, King s College London, Strand, London, WC2R 2LS, UK
    Chemphyschem 12:662-72. 2011
    ....
  6. ncbi request reprint Gd-containing conjugated polymer nanoparticles: bimodal nanoparticles for fluorescence and MRI imaging
    Zeina Hashim
    Department of Physics, King s College London, Strand, London, WC2R 2LS, UK
    Nanoscale 6:8376-86. 2014
    ....
  7. ncbi request reprint Influence of molecular shape, conformability, net surface charge, and tissue interaction on transscleral macromolecular diffusion
    Nishanthan Srikantha
    Department of Physics, King s College London, United Kingdom School of Medicine, King s College London, London, United Kingdom
    Exp Eye Res 102:85-92. 2012
    ..Each variable cannot be considered in isolation, and the interplay of all these variables needs to be tested, when selecting or designing drugs for transscleral delivery...
  8. ncbi request reprint Imaging immune surveillance by T cells and NK cells
    Fiona E McCann
    Department of Biological Sciences, Sir Alexander Fleming Building, Imperial College of Science, Technology and Medicine, London, UK
    Immunol Rev 189:179-92. 2002
    ..Imaging parameters other than fluorescence intensity, such as the lifetime of the fluorophore's excited state, could be used to report on protein environments...
  9. doi request reprint Rapid wide-field photon counting imaging with microsecond time resolution
    Klaus Suhling
    Department of Physics, King s College London, Strand, London WC2R 2LS, UK
    Opt Express 18:25292-8. 2010
    ..The detection system is mounted on an inverted microscope and applied to time-resolved imaging of Europium-containing polyoxometalate nanoparticles...
  10. doi request reprint Direct binding of Grb2 SH3 domain to FGFR2 regulates SHP2 function
    Zamal Ahmed
    Department of Structural and Molecular Biology, University College London, Gower Street, London, WC1E 6BT, UK
    Cell Signal 22:23-33. 2010
    ..Synthetic peptides based on the C-terminus of FGFR2 bind to full length Grb2 with low micromolar affinity. The function of this novel mode of Grb2 binding provides resistance to site-specific Shp2-mediated receptor dephosphorylation...
  11. ncbi request reprint Imaging proteins in vivo using fluorescence lifetime microscopy
    Frederic Festy
    Randall Division of Cell and Molecular Biophysics, King s College London, London, UK SE1 1UL
    Mol Biosyst 3:381-91. 2007
    ..We review the principles and recent advances in the application of the technique, instrumentation and molecular probe development...
  12. doi request reprint Phospholipid encapsulated semiconducting polymer nanoparticles: their use in cell imaging and protein attachment
    Philip Howes
    Department of Physics, King s College London, Strand, London WC2R 2LS, UK
    J Am Chem Soc 132:3989-96. 2010
    ..Carboxylic acid functionalized SPNs were also synthesized and conjugated to bovine serum albumin (BSA) by carbodiimide-mediated chemistry, a key step in the realization of targeted imaging using conjugated polymers...
  13. doi request reprint Effect of refractive index on the fluorescence lifetime of green fluorescent protein
    Carolyn Tregidgo
    King s College London, Department of Physics, Strand, London, WC2R 2LS, United Kingdom
    J Biomed Opt 13:031218. 2008
    ..Our findings show that the GFP fluorescence lifetime is shortened in TIRF microscopy in comparison to confocal microscopy...
  14. ncbi request reprint Fluorescence probe techniques to monitor protein adsorption-induced conformation changes on biodegradable polymers
    Johan Benesch
    3B s Research Group Biomaterials, Biodegradables and Biomimetics, Department of Polymer Engineering, University of Minho, 4710 057 Braga, Portugal
    J Colloid Interface Sci 312:193-200. 2007
    ....
  15. ncbi request reprint Diffusion in a sol-gel-derived medium with a view toward biosensor applications
    Graham Hungerford
    Departamento de Fisica, Universidade do Minho, 4710 057 Braga, Portugal
    J Phys Chem B 111:3558-62. 2007
    ..Diffusion coefficients of between ca. 0.5 x 10(-7) and 4 x 10(-7) cm2 s(-1) were obtained from preliminary FRAP measurements of fluorescein and correlated to differences in the catalytic activity of HRP incorporated in the monolith...
  16. ncbi request reprint Time-resolved fluorescence anisotropy imaging applied to live cells
    Klaus Suhling
    Departments of Physics, Biological Sciences and Chemistry, Imperial College London, London SW7 2BW, UK
    Opt Lett 29:584-6. 2004
    ..We demonstrate imaging of solvent viscosity variations through the rotational correlation time of fluorescein in a multiwell plate and apply this technique to probe the microviscosity in live cells...
  17. doi request reprint Molecular rotor measures viscosity of live cells via fluorescence lifetime imaging
    Marina K Kuimova
    Chemistry Department, Imperial College London, Exhibition Road, SW7 2AZ, UK
    J Am Chem Soc 130:6672-3. 2008
    ..The high viscosity of intracellular compartments is likely to play an important role in vital intracellular processes, including the rate of diffusion of reactive oxygen species, causing programmed cell destruction...
  18. pmc Imaging the environment of green fluorescent protein
    Klaus Suhling
    Department of Biological Sciences, Sir Alexander Fleming Building, Imperial College of Science, Technology and Medicine, London SW7 1AZ, United Kingdom
    Biophys J 83:3589-95. 2002
    ..This novel approach paves the way for imaging the biophysical environment of specific GFP-tagged proteins in live cells...
  19. ncbi request reprint Molecular diffusion within sol-gel derived matrices viewed via fluorescence recovery after photobleaching
    Graham Hungerford
    Departamento de Fisica, Universidade do Minho, 4710 057, Braga, Portugal
    Photochem Photobiol Sci 6:825-8. 2007
    ....