Anne J Ridley

Summary

Affiliation: King's College London
Country: UK

Publications

  1. pmc The PI3K p110alpha isoform regulates endothelial adherens junctions via Pyk2 and Rac1
    Robert J Cain
    Randall Division of Cell and Molecular Biophysics, King s College London, London SE1 1UL, England, UK
    J Cell Biol 188:863-76. 2010
  2. pmc Rnd3 induces stress fibres in endothelial cells through RhoB
    Undine Gottesbühren
    Randall Division of Cell and Molecular Biophysics, King s College London, New Hunt s House, Guy s Campus, London SE1 1UL, UK
    Biol Open 2:210-6. 2013
  3. pmc Microtubules regulate migratory polarity through Rho/ROCK signaling in T cells
    Aya Takesono
    University College London, Department of Biochemistry and Molecular Biology and Ludwig Institute for Cancer Research, London, United Kingdom
    PLoS ONE 5:e8774. 2010
  4. pmc Coordinated RhoA signaling at the leading edge and uropod is required for T cell transendothelial migration
    Sarah J Heasman
    Randall Division of Cell and Molecular Biophysics, King s College London, London, England, UK
    J Cell Biol 190:553-63. 2010
  5. pmc RhoA and RhoC have distinct roles in migration and invasion by acting through different targets
    Francisco M Vega
    Randall Division of Cell and Molecular Biophysics, King s College London, London SE1 1UL, England, UK
    J Cell Biol 193:655-65. 2011
  6. pmc Analysis of Rho GTPase expression in T-ALL identifies RhoU as a target for Notch involved in T-ALL cell migration
    P J Bhavsar
    Randall Division of Cell and Molecular Biophysics, King s College London, London, UK
    Oncogene 32:198-208. 2013
  7. pmc Rac activation by the T-cell receptor inhibits T cell migration
    Eva Cernuda-Morollón
    Histocompatibility and Transplantation Unit, Hospital Universitario Central de Asturias, Oviedo, Spain
    PLoS ONE 5:e12393. 2010
  8. ncbi request reprint RhoA, RhoB and RhoC have different roles in cancer cell migration
    A J Ridley
    Randall Division of Cell and Molecular Biophysics, King s College London, New Hunt s House, Guy s Campus, London, UK
    J Microsc 251:242-9. 2013
  9. doi request reprint Life at the leading edge
    Anne J Ridley
    Randall Division of Cell and Molecular Biophysics, King s College London, New Hunt s House, Guy s Campus, London SE1 1UL, United Kingdom
    Cell 145:1012-22. 2011
  10. doi request reprint Historical overview of Rho GTPases
    Anne J Ridley
    Randall Division of Cell and Molecular Biophysics, King s College London, London, UK
    Methods Mol Biol 827:3-12. 2012

Collaborators

Detail Information

Publications74

  1. pmc The PI3K p110alpha isoform regulates endothelial adherens junctions via Pyk2 and Rac1
    Robert J Cain
    Randall Division of Cell and Molecular Biophysics, King s College London, London SE1 1UL, England, UK
    J Cell Biol 188:863-76. 2010
    ..We propose that p110alpha regulates endothelial barrier function by inducing the formation of a VE-cadherin-associated protein complex that coordinates changes to adherens junctions with the actin cytoskeleton...
  2. pmc Rnd3 induces stress fibres in endothelial cells through RhoB
    Undine Gottesbühren
    Randall Division of Cell and Molecular Biophysics, King s College London, New Hunt s House, Guy s Campus, London SE1 1UL, UK
    Biol Open 2:210-6. 2013
    ..These data indicate that the cytoskeletal response to Rnd3 expression is dependent on cell type and context, and identify regulation of RhoB as a new mechanism for Rnd proteins to affect the actin cytoskeleton...
  3. pmc Microtubules regulate migratory polarity through Rho/ROCK signaling in T cells
    Aya Takesono
    University College London, Department of Biochemistry and Molecular Biology and Ludwig Institute for Cancer Research, London, United Kingdom
    PLoS ONE 5:e8774. 2010
    ..Microtubules (MTs) are required for persistent migration and chemotaxis, but how they affect cell polarity is not known...
  4. pmc Coordinated RhoA signaling at the leading edge and uropod is required for T cell transendothelial migration
    Sarah J Heasman
    Randall Division of Cell and Molecular Biophysics, King s College London, London, England, UK
    J Cell Biol 190:553-63. 2010
    ..Our data indicate that RhoA activity is dynamically regulated at the front and back of T cells to coordinate TEM...
  5. pmc RhoA and RhoC have distinct roles in migration and invasion by acting through different targets
    Francisco M Vega
    Randall Division of Cell and Molecular Biophysics, King s College London, London SE1 1UL, England, UK
    J Cell Biol 193:655-65. 2011
    ..We propose that RhoA contributes to migratory cell polarity through ROCK2-mediated suppression of Rac1 activity in lamellipodia, whereas RhoC promotes polarized migration through FMNL3 by restricting lamellipodial broadening...
  6. pmc Analysis of Rho GTPase expression in T-ALL identifies RhoU as a target for Notch involved in T-ALL cell migration
    P J Bhavsar
    Randall Division of Cell and Molecular Biophysics, King s College London, London, UK
    Oncogene 32:198-208. 2013
    ..These results indicate that NOTCH1 mutation stimulates T-ALL cell migration through RhoU upregulation that could contribute to the leukaemia cell dissemination...
  7. pmc Rac activation by the T-cell receptor inhibits T cell migration
    Eva Cernuda-Morollón
    Histocompatibility and Transplantation Unit, Hospital Universitario Central de Asturias, Oviedo, Spain
    PLoS ONE 5:e12393. 2010
    ..Activation of the T-cell receptor (TCR) triggers a migration stop signal to facilitate interaction with antigen-presenting cells and cell retention at inflammatory sites, but the mechanisms responsible for this effect are not known...
  8. ncbi request reprint RhoA, RhoB and RhoC have different roles in cancer cell migration
    A J Ridley
    Randall Division of Cell and Molecular Biophysics, King s College London, New Hunt s House, Guy s Campus, London, UK
    J Microsc 251:242-9. 2013
    ..This suggests that they each make unique contributions to cancer by participating in different protein complexes. ..
  9. doi request reprint Life at the leading edge
    Anne J Ridley
    Randall Division of Cell and Molecular Biophysics, King s College London, New Hunt s House, Guy s Campus, London SE1 1UL, United Kingdom
    Cell 145:1012-22. 2011
    ..In addition, these different protrusion methods likely act in concert to move cells through complex environments in vivo...
  10. doi request reprint Historical overview of Rho GTPases
    Anne J Ridley
    Randall Division of Cell and Molecular Biophysics, King s College London, London, UK
    Methods Mol Biol 827:3-12. 2012
    ..More recent work has delineated where Rho GTPases act in cells, the molecular pathways linking some of them to specific cellular responses, and their functions in the development of multicellular organisms...
  11. doi request reprint Regulation of macrophage adhesion and migration by Rho GTP-binding proteins
    A J Ridley
    Randall Division of Cell and Molecular Biophysics, New Hunt s House, King s College London, Guy s Campus, London, UK
    J Microsc 231:518-23. 2008
    ..However, recent studies in macrophages indicate that they are not always essential for migration, although they do affect cell shape and adhesion...
  12. pmc CD73 represses pro-inflammatory responses in human endothelial cells
    Jana Kg Grünewald
    King s College London, Randall Division of Cell and Molecular Biophysics, New Hunt s House, Guy s Campus, London SE1 1UL, UK
    J Inflamm (Lond) 7:10. 2010
    ..CD73 has been reported to regulate expression of pro-inflammatory molecules in mouse endothelium. Our aim is to determine the function of CD73 in human endothelial cells...
  13. pmc RhoE inhibits 4E-BP1 phosphorylation and eIF4E function impairing cap-dependent translation
    Priam Villalonga
    Ludwig Institute for Cancer Research, University College London, W1W7BS London, United Kingdom
    J Biol Chem 284:35287-96. 2009
    ....
  14. pmc RhoE function is regulated by ROCK I-mediated phosphorylation
    Kirsi Riento
    Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, London, UK
    EMBO J 24:1170-80. 2005
    ..This is the first demonstration of an endogenous Rho family member being phosphorylated in vivo and indicates that phosphorylation is an important mechanism to control the stability and function of this GTPase-deficient Rho protein...
  15. pmc RhoB regulates cell migration through altered focal adhesion dynamics
    Francisco M Vega
    Randall Division of Cell and Molecular Biophysics, King s College London, New Hunt s House, Guy s Campus, London SE1 1UL, UK
    Open Biol 2:120076. 2012
    ..We propose that RhoB contributes to directional cell migration by regulating β1 integrin surface levels and activity, thereby stabilizing lamellipodial protrusions...
  16. pmc Cdc42 promotes transendothelial migration of cancer cells through β1 integrin
    Nicolas Reymond
    Randall Division of Cell and Molecular Biophysics, King s College London, London SE1 1UL, England, UK
    J Cell Biol 199:653-68. 2012
    ..By identifying β1 integrin as a transcriptional target of Cdc42, our results provide new insight into Cdc42 function...
  17. ncbi request reprint RhoB affects macrophage adhesion, integrin expression and migration
    Ann P Wheeler
    Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, London, UK
    Exp Cell Res 313:3505-16. 2007
    ..Since macrophages do not express RhoC, these effects of C3 transferase are due to inhibition of RhoA rather than RhoB. Our results suggest that RhoB affects cell shape and migration by regulating surface integrin levels...
  18. pmc The p110delta isoform of PI 3-kinase negatively controls RhoA and PTEN
    Evangelia A Papakonstanti
    Ludwig Institute for Cancer Research, London, UK
    EMBO J 26:3050-61. 2007
    ..This work identifies the RhoA/ROCK pathway as a major target of p110delta-mediated PI3K signalling, and establishes for the first time that PI3K controls itself, via a feedback loop involving PTEN...
  19. doi request reprint T-cell receptor- and CD28-induced Vav1 activity is required for the accumulation of primed T cells into antigenic tissue
    Rachel David
    Department of Immunology, Division of Medicine, Imperial College London, Hammersmith Campus, London, United Kingdom
    Blood 113:3696-705. 2009
    ..This novel function of APC-induced, and TCR- and CD28-mediated Vav1 activity in the regulation of effector T-cell immunity highlights its potential as a therapeutic target in T cell-mediated tissue damage...
  20. pmc Adherens junctions connect stress fibres between adjacent endothelial cells
    Jaime Millan
    University College London, Ludwig Institute for Cancer Research and Department of Biochemistry and Molecular Biology, London WC1E6BT, UK
    BMC Biol 8:11. 2010
    ..However, in the endothelium, cell-cell junctions are highly dynamic and morphologically heterogeneous...
  21. ncbi request reprint Rac1 and Rac2 regulate macrophage morphology but are not essential for migration
    Ann P Wheeler
    Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, 91 Riding House Street, London, W1W 7BS, UK
    J Cell Sci 119:2749-57. 2006
    ..Together, these data show that Rac1 and Rac2 have distinct roles in regulating cell morphology, migration and invasion, but are not essential for macrophage migration or chemotaxis...
  22. pmc 14-3-3 proteins interact with a hybrid prenyl-phosphorylation motif to inhibit G proteins
    Philippe Riou
    Randall Division of Cell and Molecular Biophysics, New Hunt s House, King s College London, London, UK
    Cell 153:640-53. 2013
    ..In contrast to the canonical GTP/GDP switch that regulates most Ras superfamily members, our results reveal an unprecedented mechanism for G protein inhibition by 14-3-3 proteins...
  23. ncbi request reprint Roles of Rho/ROCK and MLCK in TNF-alpha-induced changes in endothelial morphology and permeability
    Jenny A G McKenzie
    Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, London, United Kingdom
    J Cell Physiol 213:221-8. 2007
    ..These results suggest that the gradual increase in permeability induced by TNF-alpha does not reflect contractile mechanisms mediated by Rho, ROCK, and MLCK, but involves long-term reorganization of tight junction proteins...
  24. pmc Mechanism of multi-site phosphorylation from a ROCK-I:RhoE complex structure
    David Komander
    Section of Structural Biology, The Institute of Cancer Research, Chester Beatty Laboratories, London, UK
    EMBO J 27:3175-85. 2008
    ..We propose that ROCK-I phosphorylation of RhoE forms part of a feedback loop to regulate RhoA signalling...
  25. doi request reprint Different PI 3-kinase inhibitors have distinct effects on endothelial permeability and leukocyte transmigration
    Robert J Cain
    Randall Division of Cell and Molecular Biophysics, King s College London, New Hunt s House, Guy s Campus, London SE1 1UL, UK
    Int J Biochem Cell Biol 44:1929-36. 2012
    ..PI-103-related compounds are therefore likely to be good candidates for treating chronic inflammatory responses involving TNFα...
  26. ncbi request reprint Inhibition of ROCK by RhoE
    Kirsi Riento
    Ludwig Institute for Cancer Research, University College London, UK
    Methods Enzymol 406:533-41. 2006
    ..RhoE itself is phosphorylated by ROCK I, and this may enhance RhoE regulation of ROCK I function. This chapter describes the method used for studying ROCK inhibition by RhoE...
  27. ncbi request reprint Rho GTPases and leukocyte adhesion receptor expression and function in endothelial cells
    Eva Cernuda-Morollón
    Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, London, UK
    Circ Res 98:757-67. 2006
    ..Rho GTPases are therefore candidate targets for inhibiting leukocyte transendothelial migration in heart disease and chronic inflammatory disorders...
  28. ncbi request reprint beta1-Integrins determine the dendritic morphology which enhances DC-SIGN-mediated particle capture by dendritic cells
    Claire A Swetman Andersen
    Department of Immunology and Molecular Pathology, University College London, Windeyer Building, 46 Cleveland Street, London W1T 4JF, UK
    Int Immunol 18:1295-303. 2006
    ..The unusual combination of a dendritic morphology and high motility is likely to play a major role in the efficient function of DCs as sentinels of the immune system...
  29. pmc RhoE binds to ROCK I and inhibits downstream signaling
    Kirsi Riento
    Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, London, United Kingdom
    Mol Cell Biol 23:4219-29. 2003
    ..These data suggest that RhoE induces stress fiber disassembly by directly binding ROCK I and inhibiting it from phosphorylating downstream targets...
  30. doi request reprint Vav GEFs regulate macrophage morphology and adhesion-induced Rac and Rho activation
    Parag J Bhavsar
    King s College London, Randall Division of Cell and Molecular Biophysics, Guy s Campus, London, UK
    Exp Cell Res 315:3345-58. 2009
    ....
  31. pmc p120ctn and P-cadherin but not E-cadherin regulate cell motility and invasion of DU145 prostate cancer cells
    Sandra Kümper
    Randall Division of Cell and Molecular Biophysics, King s College London, London, United Kingdom
    PLoS ONE 5:e11801. 2010
    ..p120ctn is known to regulate cell-cell adhesion by increasing cadherin stability, but the effects of other adherens junction components on cell-cell adhesion have not been compared with that of p120ctn...
  32. ncbi request reprint Requirement for PI 3-kinase gamma in macrophage migration to MCP-1 and CSF-1
    Gareth E Jones
    Randall Centre, King s College London, New Hunt s House, Guy s Campus, London SE1 1UL, UK
    Exp Cell Res 290:120-31. 2003
    ..These results indicate that the initial actin reorganization induced by either a GPCR or tyrosine kinase receptor agonist is not dependent on PI3Kgamma, whereas PI3Kgamma is needed for optimal migration of macrophages to either agonist...
  33. pmc T cell receptor-induced phosphoinositide-3-kinase p110delta activity is required for T cell localization to antigenic tissue in mice
    Sarah J Jarmin
    Department of Immunology, Division of Medicine, Imperial College London, Hammersmith Campus, London, United Kingdom
    J Clin Invest 118:1154-64. 2008
    ..These observations suggest that pharmacological targeting of p110delta activity is a viable strategy for the therapy of T cell-mediated pathology...
  34. ncbi request reprint An in vitro model to study the role of endothelial rho GTPases during leukocyte transendothelial migration
    Jaime Millan
    Ludwig Institute for Cancer Research, University College London, UK
    Methods Enzymol 406:643-55. 2006
    ..Finally, we describe how to image and quantitate leukocyte TEM by digital time-lapse microscopy...
  35. doi request reprint Crossing the endothelial barrier during metastasis
    Nicolas Reymond
    1 Randall Division of Cell and Molecular Biophysics, King s College London, New Hunt s House, Guy s Campus, London SE1 1UL, UK 2 Centre de Recherche de Biochimie Macromoléculaire, Centre National de la Recherche Scientifique CNRS UMR5237, 1919 Route de Mende, 34293 Montpellier, Cedex 5, France 3
    Nat Rev Cancer 13:858-70. 2013
    ..Identification of the mechanisms that underlie cancer cell extravasation could lead to the development of new therapies to reduce metastasis. ..
  36. ncbi request reprint Radixin regulates cell migration and cell-cell adhesion through Rac1
    Ferran Valderrama
    Randall Division of Cell and Molecular Biophysics, King s College London, London, UK
    J Cell Sci 125:3310-9. 2012
    ..Our results indicate that radixin plays an important role in promoting cell migration by regulating Rac1-mediated epithelial polarity and formation of adherens junctions through Vav GEFs...
  37. pmc PAK1-mediated activation of ERK1/2 regulates lamellipodial dynamics
    Stephen D Smith
    Ludwig Institute for Cancer Research, University College London, 91 Riding House Street, London W1W 7BS, UK
    J Cell Sci 121:3729-36. 2008
    ..Together, these data indicate that PAK1 signals via ERK1/2 to regulate lamellipodial stability...
  38. pmc Identification and characterization of a set of conserved and new regulators of cytoskeletal organization, cell morphology and migration
    Siau Wei Baï
    Randall Division of Cell and Molecular Biophysics, King s College London, New Hunt s House, Guy s Campus, London SE11UL, UK
    BMC Biol 9:54. 2011
    ..Cell migration is essential during development and in human disease progression including cancer. Most cell migration studies concentrate on known or predicted components of migration pathways...
  39. ncbi request reprint Extension, retraction and contraction in the formation of a dendritic cell dendrite: distinct roles for Rho GTPases
    Claire A Swetman
    Department of Immunology and Molecular Pathology, University College London, London, GB
    Eur J Immunol 32:2074-83. 2002
    ..DC therefore coordinate adhesion and protrusion to perform a specialized process of cellular morphogenesis, which differentiates these cells from all other cells of the immune system and may contribute to their distinctive function...
  40. ncbi request reprint Rho proteins and cancer
    Anne J Ridley
    Department of Biochemistry and Molecular Biology, Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, University College London, London, UK
    Breast Cancer Res Treat 84:13-9. 2004
    ..However, there is little evidence so far from animal studies to define if and how Rho proteins contribute to cancer cell proliferation, survival, invasion and metastasis...
  41. pmc Ezrin/radixin/moesin proteins and Rho GTPase signalling in leucocytes
    Aleksandar Ivetic
    Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, London, UK
    Immunology 112:165-76. 2004
    ..This review discusses recent findings on ERM protein function in leucocytes and how these may be integrated with Rho GTPase signalling...
  42. doi request reprint Rnd proteins: multifunctional regulators of the cytoskeleton and cell cycle progression
    Philippe Riou
    Randall Division of Cell and Molecular Biophysics, King s College London, London, UK
    Bioessays 32:986-92. 2010
    ..We propose that Rnd3 could act as a tumour suppressor to limit proliferation, but when mutations bypass this activity of Rnd3, it can promote cancer invasion through its effects in the actin cytoskeleton...
  43. doi request reprint Rho GTPases and cancer cell transendothelial migration
    Nicolas Reymond
    Randall Division of Cell and Molecular Biophysics, King s College London, London, UK
    Methods Mol Biol 827:123-42. 2012
    ..In this chapter we describe methods to assess how Rho GTPases affect the different steps of cancer cell transendothelial cell migration in vitro...
  44. doi request reprint Statins inhibit T-acute lymphoblastic leukemia cell adhesion and migration through Rap1b
    Elvira Infante
    Randall Division of Cell and Molecular Biophysics, New Hunt s House, Guy s Campus, King s College London, London SE1 1UL, UK
    J Leukoc Biol 89:577-86. 2011
    ..These results demonstrate for the first time that the closely related Rap1a and Rap1b isoforms have different functions and suggest that statins or Rap1b depletion could be used to reduce tissue invasion in T-ALL...
  45. ncbi request reprint RhoA activation by hypoxia in pulmonary arterial smooth muscle cells is age and site specific
    Karine Bailly
    Vascular Biology and Pharmacology Unit, Institute of Child Health, University College London, UK
    Circ Res 94:1383-91. 2004
    ..These data show that acute hypoxia induces RhoA activation only in PASMCs from young animals, whereas chronic hypoxia selectively downregulates RhoA activity in outer medial PASMCs leading to an altered phenotype...
  46. pmc Roles of Rho GTPases in leucocyte and leukaemia cell transendothelial migration
    Elvira Infante
    Randall Division of Cell and Molecular Biophysics, King s College London, New Hunt s House, Guy s Campus, London SE1 1UL, UK
    Philos Trans R Soc Lond B Biol Sci 368:20130013. 2013
    ..We discuss how inhibitors of Rho signalling could be used to reduce leucocyte or leukaemia cell entry into tissues. ..
  47. ncbi request reprint Intercellular adhesion molecule (ICAM)-1, but not ICAM-2, activates RhoA and stimulates c-fos and rhoA transcription in endothelial cells
    Paul W Thompson
    Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, London, United Kingdom
    J Immunol 169:1007-13. 2002
    ..These results indicate that in endothelial cells, ICAM-1, but not ICAM-2, rapidly stimulates signaling responses involving RhoA...
  48. ncbi request reprint Pulling back to move forward
    Anne J Ridley
    Ludwig Institute for Cancer Research and Department of Biochemistry and Molecular Biology, University College London, 91 Riding House Street, London W1W 7BS, United Kingdom
    Cell 116:357-8. 2004
    ..Giannone and coworkers (this issue of Cell) describe how cells periodically pull back on these protrusions in order to sense and respond to the rigidity of their environment...
  49. ncbi request reprint Mammalian cell microinjection assay to study the function of Rho family guanosine triphosphatases
    Ritu Garg
    Ludwig Institute for Cancer Research, University College London, UK
    Methods Mol Biol 332:257-67. 2006
    ..Here, we describe the procedure for microinjecting cells with purified recombinant proteins or with expression vectors encoding proteins, and for analyzing the cells after injection...
  50. doi request reprint Mammalian Rho GTPases: new insights into their functions from in vivo studies
    Sarah J Heasman
    Randall Division of Cell and Molecular Biophysics, King s College London, Guy s Campus, London, UK
    Nat Rev Mol Cell Biol 9:690-701. 2008
    ..The recent availability of knockout mice for several members of the Rho family reveals new information about their roles in signalling to the cytoskeleton and in development...
  51. doi request reprint Phosphoinositide 3-kinases in cell migration
    Robert J Cain
    Randall Division of Cell and Molecular Biophysics, School of Biomedical and Health Sciences, King s College London, Guy s Campus, London SE1 1UL, U K
    Biol Cell 101:13-29. 2009
    ..This review describes how PI3K signalling is regulated by pro-migratory stimuli, and the diverse ways in which PI3K-mediated signal transduction contributes to different aspects of cell migration...
  52. doi request reprint PAK1 and PAK2 have different roles in HGF-induced morphological responses
    Michael D Bright
    King s College London, Randall Division of Cell and Molecular Biophysics, Guy s Campus, London, SE1 1UL, UK
    Cell Signal 21:1738-47. 2009
    ..PAK2 knockdown increases phosphorylation of PAK1, indicating that PAK2 provides a negative feedback on PAK1. We hypothesise that PAK2 acts in part via PAK1 to regulate HGF-induced scattering...
  53. doi request reprint RhoB regulates uPAR signalling
    Daniela Alfano
    Randall Division of Cell and Molecular Biophysics, King s College London, New Hunt s House, Guy s Campus, London, UK
    J Cell Sci 125:2369-80. 2012
    ..This correlated with decreased association between integrins and uPAR and reduced integrin β1 activity. Our results indicate that RhoB is a key regulator of uPAR signalling in cell adhesion, migration and invasion...
  54. doi request reprint Rho GTPases in cancer cell biology
    Francisco M Vega
    Randall Division of Cell and Molecular Biophysics, King s College London, New Hunt s House, Guy s Campus, London SE1 1UL, UK
    FEBS Lett 582:2093-101. 2008
    ..Here, we discuss how Rho GTPases could contribute to different steps of cancer progression, including proliferation, survival, invasion and metastasis...
  55. ncbi request reprint Rho proteins, PI 3-kinases, and monocyte/macrophage motility
    A J Ridley
    Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, London, UK
    FEBS Lett 498:168-71. 2001
    ..Studies of monocytes and macrophages have revealed that different Rho family members and PI 3-kinases are not functionally redundant but play unique and distinct roles in motile responses...
  56. ncbi request reprint The Rho GTPases in macrophage motility and chemotaxis
    G E Jones
    Muscle and Motility Research Centre, Randall Institute, King s College, London, UK
    Cell Adhes Commun 6:237-45. 1998
    ..This implies that Cdc42, and thus probably filopodia, are required for gradient sensing and cell polarisation in macrophages...
  57. doi request reprint Determining Rho GTPase activity by an affinity-precipitation assay
    Narendra Suryavanshi
    Randall Division of Cell and Molecular Biophysics, King s College London, London, UK
    Methods Mol Biol 1046:191-202. 2013
    ..Here we describe a biochemical technique to assess this based on affinity-precipitation of the GTP-bound form from whole cell lysates. ..
  58. pmc Effects of microinjected small GTPases on the actin cytoskeleton of human neutrophils
    Margaret M Bird
    Department of Medicine, The Rayne Institute, Royal Free and University College Medical School, London, UK
    J Anat 203:379-89. 2003
    ..Our findings demonstrate that microinjection is a valuable technique for studying the role of individual proteins in neutrophils...
  59. ncbi request reprint SnapShot: Rho family GTPases
    Francisco M Vega
    Randall Division of Cell and Molecular Biophysics, King s College London, London, UK
    Cell 129:1430. 2007
  60. ncbi request reprint Rho GTPases and cell cycle control
    Priam Villalonga
    Ludwig Institute for Cancer Research and Department of Biochemistry and Molecular Biology, University College London, London, UK
    Growth Factors 24:159-64. 2006
    ....
  61. doi request reprint Quantification of transendothelial migration using three-dimensional confocal microscopy
    Robert J Cain
    Cell Motility and Cytoskeleton Group, Randall Division of Cell and Molecular Biophysics, King s College London, London, UK
    Methods Mol Biol 769:167-90. 2011
    ....
  62. doi request reprint Using the Dunn chemotaxis chamber to analyze primary cell migration in real time
    Sanjay Chaubey
    Division of Cancer Studies, King s College London, London, UK
    Methods Mol Biol 769:41-51. 2011
    ..This is a particularly useful protocol as it can be adapted to study bone marrow-derived macrophages isolated from genetically modified mice and thus study the requirement of a specific protein in cell migration and chemotaxis...
  63. ncbi request reprint Cell migration in development and disease
    Clemens M Franz
    Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, London, United Kingdom
    Dev Cell 2:153-8. 2002
    ..A recent meeting at the Max Delbrück Center in Berlin, Germany provided a forum to discuss the molecular mechanisms of cell migration in a broad range of contexts including chemotaxis, development, immunity, and cancer...
  64. ncbi request reprint Analysis of cell migration using the Dunn chemotaxis chamber and time-lapse microscopy
    Claire M Wells
    Randall Centre, King s College London, London, UK
    Methods Mol Biol 294:31-41. 2005
    ..This is a particularly useful protocol as it can be adapted to study bone marrow-derived macrophages isolated from genetically modified mice and thus study the requirement for a specific protein in cell migration and chemotaxis...
  65. doi request reprint Analyzing the roles of Rho GTPases in cancer cell migration with a live cell imaging 3D-morphology-based assay
    Audrey Colomba
    Randall Division of Cell and Molecular Biophysics, King s College London, London, UK
    Methods Mol Biol 1120:327-37. 2014
    ..We use this method coupled to RNAi to investigate how Rho GTPases affect prostate cancer cell morphology in 3D Matrigel. ..
  66. ncbi request reprint Rho family proteins: coordinating cell responses
    A J Ridley
    Ludwig Institute for Cancer Research, Royal Free and University College Medical School, 91 Riding House Street, W1W 7BS, London, UK
    Trends Cell Biol 11:471-7. 2001
    ....
  67. ncbi request reprint Differential effects of immunosuppressive drugs on T-cell motility
    A Datta
    Department of Immunology, Division of Medicine, Imperial College London, Hammersmith Hospital Campus, London, UK
    Am J Transplant 6:2871-83. 2006
    ..Our data suggest a prominent antiinflammatory role for CsA in T-cell-mediated tissue damage, by inhibiting T-cell trafficking into tissues in addition to containing clonal expansion...
  68. pmc A role for Cdc42 in macrophage chemotaxis
    W E Allen
    Muscle and Motility Research Centre, Randall Institute, King s College London WC2B 5RL, UK
    J Cell Biol 141:1147-57. 1998
    ..We conclude that Rho and Rac are required for the process of cell migration, whereas Cdc42 is required for cells to respond to a gradient of CSF-1 but is not essential for cell locomotion...
  69. ncbi request reprint The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors
    A J Ridley
    Institute for Cancer Research, Chester Beatty Laboratories, London, England
    Cell 70:389-99. 1992
    ..These data imply that rho is essential specifically for the coordinated assembly of focal adhesions and stress fibers induced by growth factors...
  70. ncbi request reprint The small GTP-binding protein rac regulates growth factor-induced membrane ruffling
    A J Ridley
    Institute for Cancer Research, Chester Beatty Laboratories, London, England
    Cell 70:401-10. 1992
    ..We propose that rac and rho are essential components of signal transduction pathways linking growth factors to the organization of polymerized actin...
  71. ncbi request reprint Requirement for Rho GTPases and PI 3-kinases during apoptotic cell phagocytosis by macrophages
    Y Leverrier
    Ludwig Institute for Cancer Research, 91 Riding House Street, W1P 8BT, and the Department of Biochemistry and Molecular Biology, University College London, WC1E 6BT, London, United Kingdom
    Curr Biol 11:195-9. 2001
    ..These results indicate that both Rho GTPases and PI 3-kinases are involved in apoptotic cell phagocytosis but that they play distinct roles in this process...
  72. ncbi request reprint Rho and Rac but not Cdc42 regulate endothelial cell permeability
    B Wojciak-Stothard
    Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, London W1W 7BS, UK
    J Cell Sci 114:1343-55. 2001
    ..These results demonstrate that Rho and Rac act in different ways to alter endothelial barrier function, whereas Cdc42 does not affect barrier function...
  73. ncbi request reprint Rho proteins: linking signaling with membrane trafficking
    A J Ridley
    Ludwig Institute for Cancer Research, Royal Free and University College Medical School, 91 Riding House Street, London W1W 7BS, UK
    Traffic 2:303-10. 2001
    ..Rho proteins may also affect membrane trafficking by altering phosphatidylinositide composition of membrane compartments, or through interactions with microtubules...
  74. pmc 3BP-1, an SH3 domain binding protein, has GAP activity for Rac and inhibits growth factor-induced membrane ruffling in fibroblasts
    P Cicchetti
    Rockefeller University, New York, NY 10021, USA
    EMBO J 14:3127-35. 1995
    ..Thus 3BP-1 is a new and specific Rac GAP that can act in cells to counter Rac-mediated membrane ruffling. How its SH3 binding site interacts with its GAP activity remains to be understood...