Research Topics
Genomes and Genes
| Anne J RidleySummaryAffiliation: King's College London Country: UK Publications
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Detail Information
Publications
Adherens junctions connect stress fibres between adjacent endothelial cellsJaime Millan
University College London, Ludwig Institute for Cancer Research and Department of Biochemistry and Molecular Biology, London WC1E6BT, UK
BMC Biol 8:11. 2010..However, in the endothelium, cell-cell junctions are highly dynamic and morphologically heterogeneous...
Regulation of macrophage adhesion and migration by Rho GTP-binding proteinsA J Ridley
Randall Division of Cell and Molecular Biophysics, New Hunt s House, King s College London, Guy s Campus, London, UK
J Microsc 231:518-23. 2008..However, recent studies in macrophages indicate that they are not always essential for migration, although they do affect cell shape and adhesion...- Historical overview of Rho GTPasesAnne J Ridley
Randall Division of Cell and Molecular Biophysics, King s College London, London, UK
Methods Mol Biol 827:3-12. 2012..More recent work has delineated where Rho GTPases act in cells, the molecular pathways linking some of them to specific cellular responses, and their functions in the development of multicellular organisms... - Life at the leading edgeAnne J Ridley
Randall Division of Cell and Molecular Biophysics, King s College London, New Hunt s House, Guy s Campus, London SE1 1UL, United Kingdom
Cell 145:1012-22. 2011..In addition, these different protrusion methods likely act in concert to move cells through complex environments in vivo... - CD73 represses pro-inflammatory responses in human endothelial cellsJana Kg Grünewald
King s College London, Randall Division of Cell and Molecular Biophysics, New Hunt s House, Guy s Campus, London SE1 1UL, UK
J Inflamm (Lond) 7:10. 2010..CD73 has been reported to regulate expression of pro-inflammatory molecules in mouse endothelium. Our aim is to determine the function of CD73 in human endothelial cells... - T-cell receptor- and CD28-induced Vav1 activity is required for the accumulation of primed T cells into antigenic tissueRachel David
Department of Immunology, Division of Medicine, Imperial College London, Hammersmith Campus, London, United Kingdom
Blood 113:3696-705. 2009..This novel function of APC-induced, and TCR- and CD28-mediated Vav1 activity in the regulation of effector T-cell immunity highlights its potential as a therapeutic target in T cell-mediated tissue damage... - RhoE function is regulated by ROCK I-mediated phosphorylationKirsi Riento
Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, London, UK
EMBO J 24:1170-80. 2005..This is the first demonstration of an endogenous Rho family member being phosphorylated in vivo and indicates that phosphorylation is an important mechanism to control the stability and function of this GTPase-deficient Rho protein... - RhoA and RhoC have distinct roles in migration and invasion by acting through different targetsFrancisco M Vega
Randall Division of Cell and Molecular Biophysics, King s College London, London SE1 1UL, England, UK
J Cell Biol 193:655-65. 2011..We propose that RhoA contributes to migratory cell polarity through ROCK2-mediated suppression of Rac1 activity in lamellipodia, whereas RhoC promotes polarized migration through FMNL3 by restricting lamellipodial broadening... - Cdc42 promotes transendothelial migration of cancer cells through β1 integrinNicolas Reymond
Randall Division of Cell and Molecular Biophysics, King s College London, London SE1 1UL, England, UK
J Cell Biol 199:653-68. 2012..By identifying β1 integrin as a transcriptional target of Cdc42, our results provide new insight into Cdc42 function... - Different PI 3-kinase inhibitors have distinct effects on endothelial permeability and leukocyte transmigrationRobert J Cain
Randall Division of Cell and Molecular Biophysics, King s College London, New Hunt s House, Guy s Campus, London SE1 1UL, UK
Int J Biochem Cell Biol 44:1929-36. 2012..PI-103-related compounds are therefore likely to be good candidates for treating chronic inflammatory responses involving TNFα... - The p110delta isoform of PI 3-kinase negatively controls RhoA and PTENEvangelia A Papakonstanti
Ludwig Institute for Cancer Research, London, UK
EMBO J 26:3050-61. 2007..This work identifies the RhoA/ROCK pathway as a major target of p110delta-mediated PI3K signalling, and establishes for the first time that PI3K controls itself, via a feedback loop involving PTEN... - Vav GEFs regulate macrophage morphology and adhesion-induced Rac and Rho activationParag J Bhavsar
King s College London, Randall Division of Cell and Molecular Biophysics, Guy s Campus, London, UK
Exp Cell Res 315:3345-58. 2009.... - Radixin regulates cell migration and cell-cell adhesion through Rac1Ferran Valderrama
Randall Division of Cell and Molecular Biophysics, King s College London, London, UK
J Cell Sci 125:3310-9. 2012..Our results indicate that radixin plays an important role in promoting cell migration by regulating Rac1-mediated epithelial polarity and formation of adherens junctions through Vav GEFs... - Mechanism of multi-site phosphorylation from a ROCK-I:RhoE complex structureDavid Komander
Section of Structural Biology, The Institute of Cancer Research, Chester Beatty Laboratories, London, UK
EMBO J 27:3175-85. 2008..We propose that ROCK-I phosphorylation of RhoE forms part of a feedback loop to regulate RhoA signalling... 
RhoB affects macrophage adhesion, integrin expression and migrationAnn P Wheeler
Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, London, UK
Exp Cell Res 313:3505-16. 2007..Since macrophages do not express RhoC, these effects of C3 transferase are due to inhibition of RhoA rather than RhoB. Our results suggest that RhoB affects cell shape and migration by regulating surface integrin levels...- Coordinated RhoA signaling at the leading edge and uropod is required for T cell transendothelial migrationSarah J Heasman
Randall Division of Cell and Molecular Biophysics, King s College London, London, England, UK
J Cell Biol 190:553-63. 2010..Our data indicate that RhoA activity is dynamically regulated at the front and back of T cells to coordinate TEM... - p120ctn and P-cadherin but not E-cadherin regulate cell motility and invasion of DU145 prostate cancer cellsSandra Kümper
Randall Division of Cell and Molecular Biophysics, King s College London, London, United Kingdom
PLoS ONE 5:e11801. 2010..p120ctn is known to regulate cell-cell adhesion by increasing cadherin stability, but the effects of other adherens junction components on cell-cell adhesion have not been compared with that of p120ctn... - Rac1 and Rac2 regulate macrophage morphology but are not essential for migrationAnn P Wheeler
Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, 91 Riding House Street, London, W1W 7BS, UK
J Cell Sci 119:2749-57. 2006..Together, these data show that Rac1 and Rac2 have distinct roles in regulating cell morphology, migration and invasion, but are not essential for macrophage migration or chemotaxis... - Microtubules regulate migratory polarity through Rho/ROCK signaling in T cellsAya Takesono
University College London, Department of Biochemistry and Molecular Biology and Ludwig Institute for Cancer Research, London, United Kingdom
PLoS ONE 5:e8774. 2010..Microtubules (MTs) are required for persistent migration and chemotaxis, but how they affect cell polarity is not known... - Roles of Rho/ROCK and MLCK in TNF-alpha-induced changes in endothelial morphology and permeabilityJenny A G McKenzie
Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, London, United Kingdom
J Cell Physiol 213:221-8. 2007..These results suggest that the gradual increase in permeability induced by TNF-alpha does not reflect contractile mechanisms mediated by Rho, ROCK, and MLCK, but involves long-term reorganization of tight junction proteins... - The PI3K p110alpha isoform regulates endothelial adherens junctions via Pyk2 and Rac1Robert J Cain
Randall Division of Cell and Molecular Biophysics, King s College London, London SE1 1UL, England, UK
J Cell Biol 188:863-76. 2010..We propose that p110alpha regulates endothelial barrier function by inducing the formation of a VE-cadherin-associated protein complex that coordinates changes to adherens junctions with the actin cytoskeleton... - Identification and characterization of a set of conserved and new regulators of cytoskeletal organization, cell morphology and migrationSiau Wei Baï
Randall Division of Cell and Molecular Biophysics, King s College London, New Hunt s House, Guy s Campus, London SE11UL, UK
BMC Biol 9:54. 2011..Cell migration is essential during development and in human disease progression including cancer. Most cell migration studies concentrate on known or predicted components of migration pathways... - Rho GTPases and cancer cell transendothelial migrationNicolas Reymond
Randall Division of Cell and Molecular Biophysics, King s College London, London, UK
Methods Mol Biol 827:123-42. 2012..In this chapter we describe methods to assess how Rho GTPases affect the different steps of cancer cell transendothelial cell migration in vitro... - 14-3-3 proteins interact with a hybrid prenyl-phosphorylation motif to inhibit g proteinsPhilippe Riou
Randall Division of Cell and Molecular Biophysics, New Hunt s House, Guy s Campus, King s College London, London SE1 1UL, UK Protein Phosphorylation Laboratory, Cancer Research UK London Research Institute, Lincoln s Inn Fields, London WC2A 3LY, UK
Cell 153:640-53. 2013..In contrast to the canonical GTP/GDP switch that regulates most Ras superfamily members, our results reveal an unprecedented mechanism for G protein inhibition by 14-3-3 proteins... - Rho GTPases in cancer cell biologyFrancisco M Vega
Randall Division of Cell and Molecular Biophysics, King s College London, New Hunt s House, Guy s Campus, London SE1 1UL, UK
FEBS Lett 582:2093-101. 2008..Here, we discuss how Rho GTPases could contribute to different steps of cancer progression, including proliferation, survival, invasion and metastasis... - T cell receptor-induced phosphoinositide-3-kinase p110delta activity is required for T cell localization to antigenic tissue in miceSarah J Jarmin
Department of Immunology, Division of Medicine, Imperial College London, Hammersmith Campus, London, United Kingdom
J Clin Invest 118:1154-64. 2008..These observations suggest that pharmacological targeting of p110delta activity is a viable strategy for the therapy of T cell-mediated pathology... - Rnd proteins: multifunctional regulators of the cytoskeleton and cell cycle progressionPhilippe Riou
Randall Division of Cell and Molecular Biophysics, King s College London, London, UK
Bioessays 32:986-92. 2010..We propose that Rnd3 could act as a tumour suppressor to limit proliferation, but when mutations bypass this activity of Rnd3, it can promote cancer invasion through its effects in the actin cytoskeleton... - Rho GTPases and leukocyte adhesion receptor expression and function in endothelial cellsEva Cernuda-Morollón
Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, London, UK
Circ Res 98:757-67. 2006..Rho GTPases are therefore candidate targets for inhibiting leukocyte transendothelial migration in heart disease and chronic inflammatory disorders... - Statins inhibit T-acute lymphoblastic leukemia cell adhesion and migration through Rap1bElvira Infante
Randall Division of Cell and Molecular Biophysics, New Hunt s House, Guy s Campus, King s College London, London SE1 1UL, UK
J Leukoc Biol 89:577-86. 2011..These results demonstrate for the first time that the closely related Rap1a and Rap1b isoforms have different functions and suggest that statins or Rap1b depletion could be used to reduce tissue invasion in T-ALL... - The Rho GTPases in macrophage motility and chemotaxisG E Jones
Muscle and Motility Research Centre, Randall Institute, King s College, London, UK
Cell Adhes Commun 6:237-45. 1998..This implies that Cdc42, and thus probably filopodia, are required for gradient sensing and cell polarisation in macrophages... - Rho proteins, PI 3-kinases, and monocyte/macrophage motilityA J Ridley
Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, London, UK
FEBS Lett 498:168-71. 2001..Studies of monocytes and macrophages have revealed that different Rho family members and PI 3-kinases are not functionally redundant but play unique and distinct roles in motile responses... - RhoB regulates uPAR signallingDaniela Alfano
Randall Division of Cell and Molecular Biophysics, King s College London, New Hunt s House, Guy s Campus, London, UK
J Cell Sci 125:2369-80. 2012..This correlated with decreased association between integrins and uPAR and reduced integrin β1 activity. Our results indicate that RhoB is a key regulator of uPAR signalling in cell adhesion, migration and invasion... - Phosphoinositide 3-kinases in cell migrationRobert J Cain
Randall Division of Cell and Molecular Biophysics, School of Biomedical and Health Sciences, King s College London, Guy s Campus, London SE1 1UL, U K
Biol Cell 101:13-29. 2009..This review describes how PI3K signalling is regulated by pro-migratory stimuli, and the diverse ways in which PI3K-mediated signal transduction contributes to different aspects of cell migration... - Effects of microinjected small GTPases on the actin cytoskeleton of human neutrophilsMargaret M Bird
Department of Medicine, The Rayne Institute, Royal Free and University College Medical School, London, UK
J Anat 203:379-89. 2003..Our findings demonstrate that microinjection is a valuable technique for studying the role of individual proteins in neutrophils... - Rho proteins and cancerAnne J Ridley
Department of Biochemistry and Molecular Biology, Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, University College London, London, UK
Breast Cancer Res Treat 84:13-9. 2004..However, there is little evidence so far from animal studies to define if and how Rho proteins contribute to cancer cell proliferation, survival, invasion and metastasis... - Quantification of transendothelial migration using three-dimensional confocal microscopyRobert J Cain
Cell Motility and Cytoskeleton Group, Randall Division of Cell and Molecular Biophysics, King s College London, London, UK
Methods Mol Biol 769:167-90. 2011.... - Using the Dunn chemotaxis chamber to analyze primary cell migration in real timeSanjay Chaubey
Division of Cancer Studies, King s College London, London, UK
Methods Mol Biol 769:41-51. 2011..This is a particularly useful protocol as it can be adapted to study bone marrow-derived macrophages isolated from genetically modified mice and thus study the requirement of a specific protein in cell migration and chemotaxis... - Rho GTPases and cell cycle controlPriam Villalonga
Ludwig Institute for Cancer Research and Department of Biochemistry and Molecular Biology, University College London, London, UK
Growth Factors 24:159-64. 2006.... - SnapShot: Rho family GTPasesFrancisco M Vega
Randall Division of Cell and Molecular Biophysics, King's College London, London, UK
Cell 129:1430. 2007 - Rnd3 induces stress fibres in endothelial cells through RhoBUndine Gottesbühren
Randall Division of Cell and Molecular Biophysics, King s College London, New Hunt s House, Guy s Campus, London SE1 1UL, UK
Biol Open 2:210-6. 2013..These data indicate that the cytoskeletal response to Rnd3 expression is dependent on cell type and context, and identify regulation of RhoB as a new mechanism for Rnd proteins to affect the actin cytoskeleton... - RhoB regulates cell migration through altered focal adhesion dynamicsFrancisco M Vega
Randall Division of Cell and Molecular Biophysics, King s College London, New Hunt s House, Guy s Campus, London SE1 1UL, UK
Open Biol 2:120076. 2012..We propose that RhoB contributes to directional cell migration by regulating β1 integrin surface levels and activity, thereby stabilizing lamellipodial protrusions... - Analysis of cell migration using the Dunn chemotaxis chamber and time-lapse microscopyClaire M Wells
Randall Centre, King's College London, London, UK
Methods Mol Biol 294:31-41. 2005..This is a particularly useful protocol as it can be adapted to study bone marrow-derived macrophages isolated from genetically modified mice and thus study the requirement for a specific protein in cell migration and chemotaxis... - Ezrin/radixin/moesin proteins and Rho GTPase signalling in leucocytesAleksandar Ivetic
Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, London, UK
Immunology 112:165-76. 2004..This review discusses recent findings on ERM protein function in leucocytes and how these may be integrated with Rho GTPase signalling... - Mammalian Rho GTPases: new insights into their functions from in vivo studiesSarah J Heasman
Randall Division of Cell and Molecular Biophysics, King s College London, Guy s Campus, London, UK
Nat Rev Mol Cell Biol 9:690-701. 2008..The recent availability of knockout mice for several members of the Rho family reveals new information about their roles in signalling to the cytoskeleton and in development... - PAK1 and PAK2 have different roles in HGF-induced morphological responsesMichael D Bright
King s College London, Randall Division of Cell and Molecular Biophysics, Guy s Campus, London, SE1 1UL, UK
Cell Signal 21:1738-47. 2009..PAK2 knockdown increases phosphorylation of PAK1, indicating that PAK2 provides a negative feedback on PAK1. We hypothesise that PAK2 acts in part via PAK1 to regulate HGF-induced scattering... - Requirement for PI 3-kinase gamma in macrophage migration to MCP-1 and CSF-1Gareth E Jones
Randall Centre, King s College London, New Hunt s House, Guy s Campus, London SE1 1UL, UK
Exp Cell Res 290:120-31. 2003..These results indicate that the initial actin reorganization induced by either a GPCR or tyrosine kinase receptor agonist is not dependent on PI3Kgamma, whereas PI3Kgamma is needed for optimal migration of macrophages to either agonist... - RhoE binds to ROCK I and inhibits downstream signalingKirsi Riento
Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, London, United Kingdom
Mol Cell Biol 23:4219-29. 2003..These data suggest that RhoE induces stress fiber disassembly by directly binding ROCK I and inhibiting it from phosphorylating downstream targets... - Rho family proteins: coordinating cell responsesA J Ridley
Ludwig Institute for Cancer Research, Royal Free and University College Medical School, 91 Riding House Street, W1W 7BS, London, UK
Trends Cell Biol 11:471-7. 2001.... - Cell migration in development and diseaseClemens M Franz
Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, London, United Kingdom
Dev Cell 2:153-8. 2002..A recent meeting at the Max Delbrück Center in Berlin, Germany provided a forum to discuss the molecular mechanisms of cell migration in a broad range of contexts including chemotaxis, development, immunity, and cancer... - Differential effects of immunosuppressive drugs on T-cell motilityA Datta
Department of Immunology, Division of Medicine, Imperial College London, Hammersmith Hospital Campus, London, UK
Am J Transplant 6:2871-83. 2006..Our data suggest a prominent antiinflammatory role for CsA in T-cell-mediated tissue damage, by inhibiting T-cell trafficking into tissues in addition to containing clonal expansion... - Rho proteins: linking signaling with membrane traffickingA J Ridley
Ludwig Institute for Cancer Research, Royal Free and University College Medical School, 91 Riding House Street, London W1W 7BS, UK
Traffic 2:303-10. 2001..Rho proteins may also affect membrane trafficking by altering phosphatidylinositide composition of membrane compartments, or through interactions with microtubules... - Rho and Rac but not Cdc42 regulate endothelial cell permeabilityB Wojciak-Stothard
Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, London W1W 7BS, UK
J Cell Sci 114:1343-55. 2001..These results demonstrate that Rho and Rac act in different ways to alter endothelial barrier function, whereas Cdc42 does not affect barrier function... - Requirement for Rho GTPases and PI 3-kinases during apoptotic cell phagocytosis by macrophagesY Leverrier
Ludwig Institute for Cancer Research, 91 Riding House Street, W1P 8BT, and the Department of Biochemistry and Molecular Biology, University College London, WC1E 6BT, London, United Kingdom
Curr Biol 11:195-9. 2001..These results indicate that both Rho GTPases and PI 3-kinases are involved in apoptotic cell phagocytosis but that they play distinct roles in this process... - The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factorsA J Ridley
Institute for Cancer Research, Chester Beatty Laboratories, London, England
Cell 70:389-99. 1992..These data imply that rho is essential specifically for the coordinated assembly of focal adhesions and stress fibers induced by growth factors... - A role for Cdc42 in macrophage chemotaxisW E Allen
Muscle and Motility Research Centre, Randall Institute, King s College London WC2B 5RL, UK
J Cell Biol 141:1147-57. 1998..We conclude that Rho and Rac are required for the process of cell migration, whereas Cdc42 is required for cells to respond to a gradient of CSF-1 but is not essential for cell locomotion... - 3BP-1, an SH3 domain binding protein, has GAP activity for Rac and inhibits growth factor-induced membrane ruffling in fibroblastsP Cicchetti
Rockefeller University, New York, NY 10021, USA
EMBO J 14:3127-35. 1995..Thus 3BP-1 is a new and specific Rac GAP that can act in cells to counter Rac-mediated membrane ruffling. How its SH3 binding site interacts with its GAP activity remains to be understood... - The small GTP-binding protein rac regulates growth factor-induced membrane rufflingA J Ridley
Institute for Cancer Research, Chester Beatty Laboratories, London, England
Cell 70:401-10. 1992..We propose that rac and rho are essential components of signal transduction pathways linking growth factors to the organization of polymerized actin...