Genomes and Genes
Affiliation: King's College London
- A novel PKC-regulated mechanism controls CD44 ezrin association and directional cell motilityJames W Legg
Department of Biological Sciences, Imperial College of Science, Technology and Medicine, Sir Alexander Fleming Building, Imperial College Road, London SW7 2AZ, UK
Nat Cell Biol 4:399-407. 2002....
- Rab25 associates with alpha5beta1 integrin to promote invasive migration in 3D microenvironmentsPatrick T Caswell
Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, UK
Dev Cell 13:496-510. 2007..These data indicate that Rab25 contributes to tumor progression by directing the localization of integrin-recycling vesicles and thereby enhancing the ability of tumor cells to invade the extracellular matrix...
- Rac regulates the interaction of fascin with protein kinase C in cell migrationMaddy Parsons
Randall Division of Cell and Molecular Biophysics, King s College London, Guy s Campus, London SE1 1UL, UK
J Cell Sci 121:2805-13. 2008..Elucidation of this novel pathway for regulation of the fascin/PKCgamma complex in migrating carcinoma cells suggests novel targets for therapeutic intervention in metastasis...
- Alpha v beta3 integrin spatially regulates VASP and RIAM to control adhesion dynamics and migrationDaniel C Worth
Randall Division of Cell and Molecular Biophysics, King s College London, London SE1 1UL, England, UK
J Cell Biol 189:369-83. 2010..These data demonstrate a novel mechanism by which alphavbeta3 integrin acts to locally suppress beta1 integrin activation and regulate protrusion, adhesion dynamics, and persistent migration...
- CAR regulates epithelial cell junction stability through control of E-cadherin traffickingPenny E Morton
1 Division of Asthma, Allergy and Lung Biology, King s College London, 5th Floor Tower Wing, Guy s Hospital Campus, London, United Kingdom 2 Randall Division of Cell and Molecular Biophysics, King s College London, New Hunt s House, Guys Campus, London, United Kingdom
Sci Rep 3:2889. 2013..This data demonstrates a novel role for CAR in regulating epithelial homeostasis. ..
- Integrin-specific control of focal adhesion kinase and RhoA regulates membrane protrusion and invasionPatricia Costa
Randall Division of Cell and Molecular Biophysics, King s College London, London, United Kingdom
PLoS ONE 8:e74659. 2013..This data demonstrates a specific role for β1 integrins in the modulation of a FAK-RhoA-actomyosin signaling axis to regulate cell invasion through complex ECM environments...
- A novel Rho-dependent pathway that drives interaction of fascin-1 with p-Lin-11/Isl-1/Mec-3 kinase (LIMK) 1/2 to promote fascin-1/actin binding and filopodia stabilityAsier Jayo
Randall Division of Cell and Molecular Biophysics, King s College London, Guy s Campus, London SE1 1UL, UK
BMC Biol 10:72. 2012....
- R-Ras regulates beta1-integrin trafficking via effects on membrane ruffling and endocytosisMatthew W Conklin
Dept of Pharmacology, Laboratory for Molecular Biology and University of Wisconsin Carbone Cancer Center, University of Wisconsin, 1525 Linden Dr, Madison, WI 53706, USA
BMC Cell Biol 11:14. 2010..Nor is much known about the spatiotemporal relationship between these two molecules, an understanding of which may provide insight into R-Ras regulation of integrins...
- Quantification of integrin receptor agonism by fluorescence lifetime imagingMaddy Parsons
Randall Division of Cell and Molecular Biophysics, King s College London, New Hunt s House, Guys Campus, London, SE1 1UL, UK
J Cell Sci 121:265-71. 2008..This study provides insight into the dependence of the activity of small molecule anti-integrin compounds upon receptor conformation, and provides a novel quantitative assay for the validation of potential integrin antagonists...
- Imaging protein-protein interactions in cell motility using fluorescence resonance energy transfer (FRET)M Parsons
Randall Centre, New Hunts House, Kings College London, Guys Campus, London SE1 1UL, UK
Biochem Soc Trans 32:431-3. 2004....
- Spatially distinct binding of Cdc42 to PAK1 and N-WASP in breast carcinoma cellsMaddy Parsons
Randall Centre, King s College London, 3rd Floor, New Hunt s House, Guy s Medical School Campus, London SE1 1UL, United Kingdom
Mol Cell Biol 25:1680-95. 2005..These data reveal for the first time distinct spatial association patterns between Cdc42 and its key effector proteins controlling cytoskeletal remodeling...
- α3β1 integrins regulate CD151 complex assembly and membrane dynamics in carcinoma cells within 3D environmentsT M E Scales
Randall Division of Cell and Molecular Biophysics, King s College London, New Hunts House, Guys Campus, London, UK
Oncogene 32:3965-79. 2013..These novel findings shed light on the complex crosstalk and switching between receptor complexes in response to different extracellular cues during cell invasion in 3D environments...
- Activated ezrin promotes cell migration through recruitment of the GEF Dbl to lipid rafts and preferential downstream activation of Cdc42Soren Prag
Richard Dimbleby Department of Cancer Research, King s College London, Guy s Medical School Campus, London SE1 1UL, United Kingdom
Mol Biol Cell 18:2935-48. 2007..Together these data suggest a mechanism whereby precise spatial guanine nucleotide exchange of Cdc42 by Dbl is dependent on functional ERM proteins and is important for directional cell migration...
- Using FRET to analyse signals controlling cell adhesion and migrationK Kemp-O'Brien
Randall Division of Cell and Molecular Biophysics, King s College London, London SE1 1UL, UK
J Microsc 251:270-8. 2013..Here, we provide an overview of some of the recent studies that have developed such probes and examples of how these biosensors have been used to further our understanding of cell migration signalling. ..
- HS1-associated protein X-1 regulates carcinoma cell migration and invasion via clathrin-mediated endocytosis of integrin alphavbeta6Alan G Ramsay
Centre for Tumour Biology, Institute of Cancer and Cancer Research UK Clinical Centre, London, United Kingdom
Cancer Res 67:5275-84. 2007..This report establishes that integrin endocytosis is required for alphavbeta6-dependent carcinoma cell motility and invasion and suggests that this process is an important mechanism in cancer progression...
- MyosinV controls PTEN function and neuronal cell sizeMichiel T van Diepen
MRC Centre for Developmental Neurobiology, New Hunt s House, King s College London, London SE1 1UL, UK
Nat Cell Biol 11:1191-6. 2009..Our data demonstrate a myosin-based transport mechanism that regulates PTEN function, providing new insights into the signalling networks regulating cell growth...
- PAK4: a pluripotent kinase that regulates prostate cancer cell adhesionClaire M Wells
Division of Cancer Studies, New Hunts House, Guy s Campus, King s College London, London SE1 1UL, UK
J Cell Sci 123:1663-73. 2010..Furthermore, we demonstrate that PAK4 can regulate RhoA activity via GEF-H1. Our results suggest that PAK4 is a pluripotent kinase that can regulate both actin cytoskeletal rearrangement and focal-adhesion dynamics...
- Coxsackie adenovirus receptor (CAR) regulates integrin function through activation of p44/42 MAPKCharlotte Farmer
Division of Asthma, Allergy and Lung Biology, King s College London School of Medicine, 5th Floor Tower Wing, Guy s Hospital Campus, London, UK
Exp Cell Res 315:2637-47. 2009..This data for the first time demonstrates that signalling downstream of CAR can have a dual effect on integrins and CAR itself in order to promote efficient viral binding to cell membranes...
- The role of kindlins in cell biology and relevance to human diseaseJ E Lai-Cheong
St John s Institute of Dermatology, King s College London Guy s Campus, London, United Kingdom
Int J Biochem Cell Biol 42:595-603. 2010..In this review, we focus on the cellular functions of the kindlins and their clinical relevance...
- Loss-of-function FERMT1 mutations in kindler syndrome implicate a role for fermitin family homolog-1 in integrin activationJoey E Lai-Cheong
St John s Institute of Dermatology, King s College London, London, United Kingdom
Am J Pathol 175:1431-41. 2009..These findings collectively provide novel data on the role of fermitin family homolog-1 in skin and further insight into the pathophysiology of Kindler syndrome...
- Defects in cell spreading and ERK1/2 activation in fibroblasts with lamin A/C mutationsLindsay J Emerson
The Randall Division of Cell and Molecular Biophysics, King s College, New Hunts House, Guy s Campus, London, SE1 1UL, UK
Biochim Biophys Acta 1792:810-21. 2009..We conclude that a functional emerin-lamin A/C complex is required for cell spreading and proliferation, possibly acting through ERK1/2 signalling...
- Adhesion dynamics: mechanisms and measurementsDaniel C Worth
Randall Division of Cell and Molecular Biophysics, Kings College London, New Hunts House, Guys Campus, London SE1 1UL, UK
Int J Biochem Cell Biol 40:2397-409. 2008..The caveats of employing these imaging techniques, as well as future directions will also be discussed...
- Targeting of the F-actin-binding protein drebrin by the microtubule plus-tip protein EB3 is required for neuritogenesisSara Geraldo
The MRC Centre for Developmental Neurobiology, New Hunt s House, Guy s Campus, King s College London, London SE11UL, UK
Nat Cell Biol 10:1181-9. 2008..When this interaction is disrupted, the formation of growth cones and the extension of neurites are impaired. We conclude that drebrin targets EB3 to coordinate F-actin-microtubule interactions that underlie neuritogenesis...
- Live cell imaging analysis of receptor functionDaniel C Worth
Randall Division of Cell and Molecular Biophysics, Kings College London, London, UK
Methods Mol Biol 591:311-23. 2010..Here we describe several common currently used techniques to study receptor behaviour in living cells...
- Site-directed perturbation of protein kinase C- integrin interaction blocks carcinoma cell chemotaxisMaddy Parsons
Richard Dimbleby Cancer Research UK Department of Cancer Research, GKT School of Medicine, St Thomas Hospital, London SE1 7EH, United Kingdom
Mol Cell Biol 22:5897-911. 2002..Importantly, our findings outline a new concept as to how carcinoma cell chemotaxis is enhanced and provide a conceptual basis for interfering with tumor cell dissemination...
- Intracellular coupling of adhesion receptors: molecular proximity measurementsMaddy Parsons
Richard Dimbleby Department of Cancer Research Cancer Research UK Labs, Rayne Institute, St Thomas Hospital, London SE1 7EH, United Kingdom
Methods Cell Biol 69:261-78. 2002
- Interaction of fascin and protein kinase Calpha: a novel intersection in cell adhesion and motilityN Anilkumar
MRC Laboratory for Molecular Cell Biology and Department of Biochemistry and Molecular Biology, University College London, London WC1E 6BT, UK
EMBO J 22:5390-402. 2003..These results identify the fascin-PKCalpha interaction as an important novel intersection in the regulation and networking of cell-matrix contacts...
- Differential activation of the PI 3-kinase effectors AKT/PKB and p70 S6 kinase by compound 48/80 is mediated by PKCalphaRichard D Byrne
Division of Cell and Molecular Biology, Imperial College, London SW7 2AZ, United Kingdom
Cell Signal 19:321-9. 2007..The latter is only activated by higher doses of c48/80 resulting in an inhibition of the c48/80 induced PKB phosphorylation, thus explaining the observed biphasic activation profile for PKB in response to this secretagogue...
- Directional migration of neural crest cells in vivo is regulated by Syndecan-4/Rac1 and non-canonical Wnt signaling/RhoAHelen K Matthews
Department of Anatomy and Development Biology, University College London, Gower Street, London WC1E 6BT, UK
Development 135:1771-80. 2008..In addition, we show that RhoA inhibits Rac in NC cells. We present a model in which Syn4 and PCP control directional NC migration by, at least in part, regulating membrane protrusions through the regulation of small GTPase activities...
- Analysis of the interaction between RGD-expressing adenovirus type 5 fiber knob domains and alphavbeta3 integrin reveals distinct binding profiles and intracellular traffickingRosie Lord
Division of Asthma Allergy and Lung Biology, King s College London School of Medicine at Guy s Kings and St Thomas Hospitals, Fifth Floor Thomas Guy House, Guy s Hospital, St Thomas Street, London SE1 9RT, UK
J Gen Virol 87:2497-505. 2006....
- Dual actin-bundling and protein kinase C-binding activities of fascin regulate carcinoma cell migration downstream of Rac and contribute to metastasisYosuke Hashimoto
Department of Cell Biology, Lerner Research Institute, The Cleveland Clinic, Cleveland, OH 44195, USA
Mol Biol Cell 18:4591-602. 2007..Thus, fascin contributes to carcinoma migration and metastasis through dual pathways that impact on multiple subcellular structures needed for cell migration...