Volker M Arlt

Summary

Affiliation: King's College London
Country: UK

Publications

  1. Reed L, Indra R, Mrízová I, Moserova M, Schmeiser H, Wolf C, et al. Application of hepatic cytochrome b5/P450 reductase null (HBRN) mice to study the role of cytochrome b5 in the cytochrome P450-mediated bioactivation of the anticancer drug ellipticine. Toxicol Appl Pharmacol. 2019;: pubmed publisher
    ..No difference in adduct formation was observed in the presence of NADH. Our study demonstrates that Cyb5 contributes to the P450-mediated bioactivation of ellipticine in vitro, but not in vivo. ..
  2. Stiborova M, Frei E, Schmeiser H, Arlt V, Martinek V. Mechanisms of enzyme-catalyzed reduction of two carcinogenic nitro-aromatics, 3-nitrobenzanthrone and aristolochic acid I: Experimental and theoretical approaches. Int J Mol Sci. 2014;15:10271-95 pubmed publisher
    ..The results summarized in this review demonstrate that a combination of experimental and theoretical approaches is a useful tool to study the enzyme-mediated reaction mechanisms of 3-NBA and AAI reduction. ..
  3. Stiborová M, Černá V, Moserová M, Mrízová I, Arlt V, Frei E. The anticancer drug ellipticine activated with cytochrome P450 mediates DNA damage determining its pharmacological efficiencies: studies with rats, Hepatic Cytochrome P450 Reductase Null (HRN™) mice and pure enzymes. Int J Mol Sci. 2014;16:284-306 pubmed publisher
  4. Imanikia S, Galea F, Nagy E, Phillips D, Sturzenbaum S, Arlt V. The application of the comet assay to assess the genotoxicity of environmental pollutants in the nematode Caenorhabditis elegans. Environ Toxicol Pharmacol. 2016;45:356-61 pubmed publisher
    ..In summary, our study provides a protocol to evaluate DNA damage of environmental pollutants in whole nematodes using the comet assay. ..
  5. Willis A, Indra R, Wohak L, Sozeri O, Feser K, Mrízová I, et al. The impact of chemotherapeutic drugs on the CYP1A1-catalysed metabolism of the environmental carcinogen benzo[a]pyrene: Effects in human colorectal HCT116 TP53(+/+), TP53(+/-) and TP53(-/-) cells. Toxicology. 2018;398-399:1-12 pubmed publisher
    ..Beside gene-environment interactions, more considerations should be given to potential drug-environment interactions during chemotherapy. ..
  6. Stiborová M, Bárta F, Levová K, Hodek P, Schmeiser H, Arlt V, et al. A Mechanism of O-Demethylation of Aristolochic Acid I by Cytochromes P450 and Their Contributions to This Reaction in Human and Rat Livers: Experimental and Theoretical Approaches. Int J Mol Sci. 2015;16:27561-75 pubmed publisher
    ..Our results indicate that utilization of experimental and theoretical methods is an appropriate study design to examine the CYP-catalyzed reaction mechanisms of AAI oxidation and contributions of human hepatic CYPs to this metabolism. ..
  7. Milichovský J, Bárta F, Schmeiser H, Arlt V, Frei E, Stiborová M, et al. Active Site Mutations as a Suitable Tool Contributing to Explain a Mechanism of Aristolochic Acid I Nitroreduction by Cytochromes P450 1A1, 1A2 and 1B1. Int J Mol Sci. 2016;17:213 pubmed publisher
    ..Our experimental model confirms the importance of the hydroxyl group possessing amino acids in the active center of CYP1A1 and 1A2 for AAI nitroreduction. ..
  8. Cerná T, Hrabeta J, Eckschlager T, Frei E, Schmeiser H, Arlt V, et al. The Histone Deacetylase Inhibitor Valproic Acid Exerts a Synergistic Cytotoxicity with the DNA-Damaging Drug Ellipticine in Neuroblastoma Cells. Int J Mol Sci. 2018;19: pubmed publisher
    ..The results are promising for in vivo studies and perhaps later for clinical studies of combined treatment of children suffering from high-risk NBL. ..
  9. Arlt V, Poirier M, Sykes S, John K, Moserova M, Stiborova M, et al. Exposure to benzo[a]pyrene of Hepatic Cytochrome P450 Reductase Null (HRN) and P450 Reductase Conditional Null (RCN) mice: Detection of benzo[a]pyrene diol epoxide-DNA adducts by immunohistochemistry and 32P-postlabelling. Toxicol Lett. 2012;213:160-6 pubmed publisher

More Information

Publications15

  1. Wohak L, Krais A, Kucab J, Stertmann J, Øvrebø S, Seidel A, et al. Carcinogenic polycyclic aromatic hydrocarbons induce CYP1A1 in human cells via a p53-dependent mechanism. Arch Toxicol. 2016;90:291-304 pubmed publisher
    ..This study demonstrates a new pathway for CYP1A1 induction by environmental PAHs and reveals an emerging role for p53 in xenobiotic metabolism. ..
  2. Arlt V, Krais A, Godschalk R, Riffo Vasquez Y, Mrízová I, Roufosse C, et al. Pulmonary Inflammation Impacts on CYP1A1-Mediated Respiratory Tract DNA Damage Induced by the Carcinogenic Air Pollutant Benzo[a]pyrene. Toxicol Sci. 2015;146:213-25 pubmed publisher
    ..Cyp1a1 appears to be involved in both BaP bioactivation and detoxification although the contribution of other enzymes to BaP-DNA adduct formation in lung and liver under inflammatory conditions remains to be explored. ..
  3. Krais A, Speksnijder E, Melis J, Indra R, Moserova M, Godschalk R, et al. The impact of p53 on DNA damage and metabolic activation of the environmental carcinogen benzo[a]pyrene: effects in Trp53(+/+), Trp53(+/-) and Trp53(-/-) mice. Arch Toxicol. 2016;90:839-51 pubmed publisher
    ..However, our results also suggest that the mechanisms involved in the altered expression and activity of the CYP1A1 enzyme by p53 in vitro and in vivo are different. ..
  4. Arlt V, Meinl W, Florian S, Nagy E, Bárta F, Thomann M, et al. Impact of genetic modulation of SULT1A enzymes on DNA adduct formation by aristolochic acids and 3-nitrobenzanthrone. Arch Toxicol. 2017;91:1957-1975 pubmed publisher
    ..Our results indicate that sulfo-conjugation catalysed by human SULT1A1 does not play a role in the activation pathways of AAI and AAII in vivo, but is important in 3-NBA bioactivation. ..
  5. Stiborova M, Arlt V, Schmeiser H. DNA Adducts Formed by Aristolochic Acid Are Unique Biomarkers of Exposure and Explain the Initiation Phase of Upper Urothelial Cancer. Int J Mol Sci. 2017;18: pubmed publisher
  6. Reed L, Mrízová I, Bárta F, Indra R, Moserova M, Kopka K, et al. Cytochrome b 5 impacts on cytochrome P450-mediated metabolism of benzo[a]pyrene and its DNA adduct formation: studies in hepatic cytochrome b 5 /P450 reductase null (HBRN) mice. Arch Toxicol. 2018;92:1625-1638 pubmed publisher
    ..Our results demonstrate that POR and cytochrome b 5 both modulate P450-mediated activation of BaP in vitro. However, hepatic P450 enzymes in vivo appear to be more important for BaP detoxification than its activation. ..