David Baulcombe

Summary

Affiliation: John Innes Centre
Country: UK

Publications

  1. ncbi David Baulcombe
    David Baulcombe
    The Sainsbury Laboratory, John Innes Centre, UK
    Curr Biol 17:R73-4. 2007
  2. ncbi Molecular biology. Amplified silencing
    David C Baulcombe
    Sainsbury Laboratory, John Innes Centre, Norwich NR4 7UH, UK
    Science 315:199-200. 2007
  3. ncbi RNA silencing in plants
    David Baulcombe
    The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK
    Nature 431:356-63. 2004
  4. ncbi DNA events. An RNA microcosm
    David Baulcombe
    Sainsbury Laboratory, John Innes Centre, Norwich NR4 7UH, UK
    Science 297:2002-3. 2002
  5. pmc Two classes of short interfering RNA in RNA silencing
    Andrew Hamilton
    The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK
    EMBO J 21:4671-9. 2002
  6. doi Isolation and cloning of small RNAs from virus-infected plants
    Louise Chappell
    The Sainsbury Laboratory, John Innes Centre, Colney, UK
    Curr Protoc Microbiol . 2006
  7. ncbi An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus
    Olivier Voinnet
    The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK
    Plant J 33:949-56. 2003
  8. ncbi RNA silencing
    David Baulcombe
    The Sainsbury Laboratory, John Innes Centre, Norwich NR4 7UH, UK
    Curr Biol 12:R82-4. 2002
  9. ncbi Viral suppression of systemic silencing
    David Baulcombe
    The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK
    Trends Microbiol 10:306-8. 2002
  10. pmc An RNA-dependent RNA polymerase prevents meristem invasion by potato virus X and is required for the activity but not the production of a systemic silencing signal
    Frank Schwach
    Sainsbury Laboratory, Norwich NR4 7UH, United Kingdom
    Plant Physiol 138:1842-52. 2005

Collaborators

  • Alan J Herr
  • Olivier Voinnet
  • Attila Molnar
  • Susana Rivas
  • Leif Schauser
  • Isabel Bäurle
  • Hailing Jin
  • David J Studholme
  • Tzahi Arazi
  • Louise Jones
  • Nicholas P Harberd
  • Ken Shirasu
  • Jane E Parker
  • J Maxwell Dow
  • Julia Kehr
  • Jack R Peart
  • Rui Lu
  • Pere Mestre
  • Louise Chappell
  • Peter Moffett
  • Garry Farnham
  • Rebecca A Mosher
  • Isabelle Malcuit
  • Ana M Martín-Hernández
  • Frank Schwach
  • Gianinna Brigneti
  • Fabian E Vaistij
  • Abdelhafid Bendahmane
  • Anja Buhtz
  • Iain R Searle
  • Wladimir I L Tameling
  • Nicolas Baumberger
  • Lisa M Smith
  • Mali Talmor-Neiman
  • Elizabeth H Bayne
  • Patrick Achard
  • Jonathan D G Jones
  • Jack Peart
  • Truus E M Abbink
  • Andrew Hamilton
  • Franziska Springer
  • Iain Searle
  • Ericka Havecker
  • Ching Hsui Tsai
  • Faridoon K Yousafzai
  • Charles W Melnyk
  • Nataliya Yelina
  • Craig S Pikaard
  • Olga Pontes
  • Miranda Lie
  • Ran Stav
  • Liron Klipcan
  • Kobi Buxdorf
  • Daria V Rakitina
  • Sergey Y Morozov
  • Barbara Baker
  • Judy Chen
  • Ai Jiuan Wu
  • Maria T Ruiz
  • Ana Montserrat Martin-Hernandez
  • Louise Day
  • Marcus C Durrant
  • John P Rathjen
  • Max Dow
  • Shunyuan Xiao
  • Graeme Cook
  • Bart J Feys
  • Thera N M Mos
  • Mark J Coleman
  • John F Bol
  • Huub J M Linthorst
  • Daniel A W Jaggard
  • Ari Sadanandom
  • David C Brice

Detail Information

Publications38

  1. ncbi David Baulcombe
    David Baulcombe
    The Sainsbury Laboratory, John Innes Centre, UK
    Curr Biol 17:R73-4. 2007
  2. ncbi Molecular biology. Amplified silencing
    David C Baulcombe
    Sainsbury Laboratory, John Innes Centre, Norwich NR4 7UH, UK
    Science 315:199-200. 2007
  3. ncbi RNA silencing in plants
    David Baulcombe
    The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK
    Nature 431:356-63. 2004
    ..We are now in a good position to investigate the full extent of this functional diversity in genetic and epigenetic mechanisms of genome control...
  4. ncbi DNA events. An RNA microcosm
    David Baulcombe
    Sainsbury Laboratory, John Innes Centre, Norwich NR4 7UH, UK
    Science 297:2002-3. 2002
  5. pmc Two classes of short interfering RNA in RNA silencing
    Andrew Hamilton
    The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK
    EMBO J 21:4671-9. 2002
    ..These findings reveal an unexpected level of complexity in the RNA silencing pathway in plants that may also apply in animals...
  6. doi Isolation and cloning of small RNAs from virus-infected plants
    Louise Chappell
    The Sainsbury Laboratory, John Innes Centre, Colney, UK
    Curr Protoc Microbiol . 2006
    ..This unit describes an isotope-free small-RNA cloning procedure that utilizes unmodified small RNAs and is routinely used to characterize small RNAs from various plant tissues...
  7. ncbi An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus
    Olivier Voinnet
    The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK
    Plant J 33:949-56. 2003
    ....
  8. ncbi RNA silencing
    David Baulcombe
    The Sainsbury Laboratory, John Innes Centre, Norwich NR4 7UH, UK
    Curr Biol 12:R82-4. 2002
  9. ncbi Viral suppression of systemic silencing
    David Baulcombe
    The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK
    Trends Microbiol 10:306-8. 2002
    ..The antiviral role of silencing might also apply in animals because a suppressor of silencing encoded by an insect virus was identified recently...
  10. pmc An RNA-dependent RNA polymerase prevents meristem invasion by potato virus X and is required for the activity but not the production of a systemic silencing signal
    Frank Schwach
    Sainsbury Laboratory, Norwich NR4 7UH, United Kingdom
    Plant Physiol 138:1842-52. 2005
    ..According to this idea, the secondary siRNAs mediate RNA silencing as an immediate response that slows down the systemic spreading of the virus into the growing point and newly emerging leaves...
  11. pmc Defective RNA processing enhances RNA silencing and influences flowering of Arabidopsis
    Alan J Herr
    Sainsbury Laboratory, John Innes Centre, Norwich NR4 7UH, United Kingdom
    Proc Natl Acad Sci U S A 103:14994-5001. 2006
    ..According to this proposal, in the absence of these ESP proteins, these RNAs have aberrant 3' termini. The aberrant RNAs would enter the RNA silencing pathways because they are converted into dsRNA by RNA-dependent RNA polymerases...
  12. pmc Spreading of RNA targeting and DNA methylation in RNA silencing requires transcription of the target gene and a putative RNA-dependent RNA polymerase
    Fabian E Vaistij
    Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, United Kingdom
    Plant Cell 14:857-67. 2002
    ..RNA silencing of ribulose-1,5-bisphosphate carboxylase/oxygenase and phytoene desaturase was not associated with the spreading of RNA targeting or DNA methylation, indicating that these endogenous RNAs are not templates for SDE1/SGS2...
  13. ncbi An EDS1 orthologue is required for N-mediated resistance against tobacco mosaic virus
    Jack R Peart
    The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK
    Plant J 29:569-79. 2002
    ..We further demonstrate that VIGS is a useful approach to dissect resistance signaling pathways in a genetically intractable plant species...
  14. ncbi Small RNAs hit the big time
    Iain R Searle
    The Sainsbury Laboratory, John Innes Centre, Norwich NR4 7UH, UK
    New Phytol 174:479-82. 2007
  15. pmc An SNF2 protein associated with nuclear RNA silencing and the spread of a silencing signal between cells in Arabidopsis
    Lisa M Smith
    Sainsbury Laboratory, John Ines Centre, Norwich NR4 7UH, United Kingdom
    Plant Cell 19:1507-21. 2007
    ..We interpret the effect of polymerase IVa and trans-acting siRNA pathway mutations in terms of a modular property of RNA silencing pathways...
  16. ncbi The Polerovirus silencing suppressor P0 targets ARGONAUTE proteins for degradation
    Nicolas Baumberger
    Sainsbury Laboratory, Norwich Research Park, NR4 7UH Norwich, United Kingdom
    Curr Biol 17:1609-14. 2007
    ..The ability of P0 to block silencing without affecting signal movement may contribute to the phloem restriction of viruses in the Polerovirus group...
  17. ncbi Widespread role for the flowering-time regulators FCA and FPA in RNA-mediated chromatin silencing
    Isabel Bäurle
    Department of Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, UK
    Science 318:109-12. 2007
    ..We propose that FCA and FPA regulate chromatin silencing of single and low-copy genes and interact in a locus-dependent manner with the canonical small interfering RNA-directed DNA methylation pathway to regulate common targets...
  18. ncbi Identification and characterization of small RNAs from the phloem of Brassica napus
    Anja Buhtz
    Department Lothar Willmitzer, Max Planck Institute of Molecular Plant Physiology, 14 476 Potsdam, Germany
    Plant J 53:739-49. 2008
    ..The strong responses in the phloem suggest a role of miRNAs in systemic information transfer via this long-distance transport system...
  19. pmc PolIVb influences RNA-directed DNA methylation independently of its role in siRNA biogenesis
    Rebecca A Mosher
    Sainsbury Laboratory, Norwich NR4 7UH, United Kingdom
    Proc Natl Acad Sci U S A 105:3145-50. 2008
    ..From these findings, we infer that the role of PolIVb in siRNA production is secondary to a role in chromatin modification and is influenced by chromatin context...
  20. ncbi Cell-to-cell movement of potato potexvirus X is dependent on suppression of RNA silencing
    Elizabeth H Bayne
    The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK
    Plant J 44:471-82. 2005
    ..It is possible, based on analyses of short interfering RNA accumulation, that P25 suppresses silencing by interfering with either assembly or function of the effector complexes of RNA silencing...
  21. ncbi Virus-induced gene silencing in plants
    Rui Lu
    The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK
    Methods 30:296-303. 2003
    ..These methods and the underlying general principles also apply when VIGS is used in the analysis of other aspects of plant biology...
  22. ncbi Modulation of floral development by a gibberellin-regulated microRNA
    Patrick Achard
    Department of Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, UK
    Development 131:3357-65. 2004
    ..We propose that miR159 is a phytohormonally regulated homeostatic modulator of GAMYB activity, and hence of GAMYB-dependent developmental processes...
  23. ncbi NRG1, a CC-NB-LRR protein, together with N, a TIR-NB-LRR protein, mediates resistance against tobacco mosaic virus
    Jack R Peart
    The Sainsbury Laboratory, John Innes Centre, Norwich, United Kingdom
    Curr Biol 15:968-73. 2005
    ..The results also indicate that, in addition to the previously recognized role in elicitor recognition, NB-LRR proteins may also function in downstream signaling pathways...
  24. ncbi Crystal structure of p19--a universal suppressor of RNA silencing
    David C Baulcombe
    The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich, NR4 7UH, UK
    Trends Biochem Sci 29:279-81. 2004
    ..They also suggest various ways that Tombusvirus suppressors can be used to investigate RNA silencing in plants and animals...
  25. pmc High throughput virus-induced gene silencing implicates heat shock protein 90 in plant disease resistance
    Rui Lu
    The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK
    EMBO J 22:5690-9. 2003
    ..This approach illustrates the potential benefits and limitations of RNA silencing in forward screens of gene function in plants...
  26. ncbi Cloning and characterization of micro-RNAs from moss
    Tzahi Arazi
    Department of Ornamental Horticulture, Agricultural Research Organization, The Volcani Center, PO Box 6, Bet Dagan 50250, Israel
    Plant J 43:837-48. 2005
    ..Our observations suggest that miRNAs play important regulatory roles in mosses...
  27. pmc Ubiquitin ligase-associated protein SGT1 is required for host and nonhost disease resistance in plants
    Jack R Peart
    The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, United Kingdom
    Proc Natl Acad Sci U S A 99:10865-9. 2002
    ..Our findings show that silencing SGT1 affects diverse types of disease resistance in plants and support the idea that R protein-mediated and nonhost resistance may involve similar mechanisms...
  28. pmc Tobacco rattle virus 16-kilodalton protein encodes a suppressor of RNA silencing that allows transient viral entry in meristems
    Ana M Martín-Hernández
    Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, United Kingdom
    J Virol 82:4064-71. 2008
    ..This novel mechanism of meristem exclusion may be associated with the phenomenon of recovery in virus-infected plants in which upper leaves have little or no virus and are immune to secondary infection by the same virus...
  29. ncbi Constitutive gain-of-function mutants in a nucleotide binding site-leucine rich repeat protein encoded at the Rx locus of potato
    Abdelhafid Bendahmane
    The Sainsbury Laboratory, John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK
    Plant J 32:195-204. 2002
    ..However activation of Rx is not simply release of negative regulation by the LRR and adjacent sequence because deleted forms of Rx that lack constitutive gain of function mutations are not active unless the protein is overexpressed...
  30. ncbi miRNAs control gene expression in the single-cell alga Chlamydomonas reinhardtii
    Attila Molnar
    Sainsbury Laboratory, John Innes Centre, Norwich NR4 7UH, UK
    Nature 447:1126-9. 2007
    ..The common features of miRNAs and siRNAs in an alga and in higher plants indicate that complex RNA-silencing systems evolved before multicellularity and were a feature of primitive eukaryotic cells...
  31. pmc Physical association of the NB-LRR resistance protein Rx with a Ran GTPase-activating protein is required for extreme resistance to Potato virus X
    Wladimir I L Tameling
    Sainsbury Laboratory, John Ines Centre, Norwich NR4 7UH, United Kingdom
    Plant Cell 19:1682-94. 2007
    ..These results implicate RanGAP-mediated cellular mechanisms, including nucleocytoplasmic trafficking, in the activation of disease resistance...
  32. ncbi Potato virus Y NIa protease activity is not sufficient for elicitation of Ry-mediated disease resistance in potato
    Pere Mestre
    The Sainsbury Laboratory, John Innes Centre, Norwich Research Park, Colney Lane, Norwich NR4 7UH, UK
    Plant J 36:755-61. 2003
    ..However, protease activity was not sufficient because we found three elicitor-defective mutants in which there was a high level of protease activity in this assay...
  33. ncbi Virus-induced gene silencing in Solanum species
    Gianinna Brigneti
    The Sainsbury Laboratory, John Innes Centre, Norwich Research Park, Colney Lane, Norwich NR4 7UH, UK
    Plant J 39:264-72. 2004
    ..Thus, the VIGS system is an effective method of rapidly assessing gene function in potato...
  34. ncbi Identification of trans-acting siRNAs in moss and an RNA-dependent RNA polymerase required for their biogenesis
    Mali Talmor-Neiman
    Department of Ornamental Horticulture, Agricultural Research Organization, The Volcani Center, PO Box 6, Bet Dagan 50250, Israel
    Plant J 48:511-21. 2006
    ..Taken together, our data indicate that contig13502 is a novel TAS locus and suggest a role for derived tasiRNAs in the regulation of gene expression in moss...
  35. pmc Artificial evolution extends the spectrum of viruses that are targeted by a disease-resistance gene from potato
    Garry Farnham
    The Sainsbury Laboratory, John Innes Centre, Norwich NR4 7UH, United Kingdom
    Proc Natl Acad Sci U S A 103:18828-33. 2006
    ..The breadth of the disease-resistance phenotype from a natural R gene may be influenced by the tradeoff between the costs and benefits of broad-spectrum disease resistance...
  36. pmc Elicitor-mediated oligomerization of the tobacco N disease resistance protein
    Pere Mestre
    Sainsbury Laboratory, John Ines Centre, Norwich NR4 7UH, United Kingdom
    Plant Cell 18:491-501. 2006
    ..However, loss-of-function mutations in the RNBS-A motif and in the TIR domain retain the ability to oligomerize. From these results, we conclude that oligomerization is an early event in the N-mediated resistance to TMV...
  37. ncbi Silencing of a gene encoding a protein component of the oxygen-evolving complex of photosystem II enhances virus replication in plants
    Truus E M Abbink
    Institute of Molecular Plant Sciences, Gorlaeus Laboratories, Leiden University, 2300 RA, The Netherlands
    Virology 295:307-19. 2002
    ..Silencing of gene #13 may reflect a novel strategy of TMV to suppress basal host defense mechanisms. The two-hybrid screenings did not identify tobacco proteins involved in helicase domain-induced N-mediated resistance...
  38. pmc Interaction between domains of a plant NBS-LRR protein in disease resistance-related cell death
    Peter Moffett
    The Sainsbury Laboratory, John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK
    EMBO J 21:4511-9. 2002
    ..We propose that activation of Rx entails sequential disruption of at least two intramolecular interactions...