Research Topics
Species | P WorkmanSummaryAffiliation: Institute of Cancer Research Country: UK Publications
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Publications
New approaches to molecular cancer therapeuticsIan Collins
Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, Haddow Laboratories, 15 Cotswold Road, Sutton, Surrey SM2 5NG, UK
Nat Chem Biol 2:689-700. 2006..We show how chemical biology approaches offer techniques for interconnecting elements of the traditional linear progression from gene to drug, thereby providing a basis for increasing speed and success in cancer drug discovery...
Personalized medicine: patient-predictive panel powerPaul Workman
Signal Transduction and Molecular Pharmacology Team, Cancer Research UK Cancer Therapeutics Unit, Division of Cancer Therapeutics, The Institute of Cancer Research, Haddow Laboratories, Sutton, UK
Cancer Cell 21:455-8. 2012..Known oncogene addictions are confirmed while surprising complexities and biomarker relationships with clinical potential are revealed...
Inhibiting the phosphoinositide 3-kinase pathway for cancer treatmentP Workman
Cancer Research UK Centre for Cancer Therapeutics, Institute of Cancer Research, Sutton, Surrey SM2 5NG, U K
Biochem Soc Trans 32:393-6. 2004..Prototype inhibitors show evidence of anticancer activity in vitro and in vivo animal models. The recent development of isoform-selective inhibitors shows considerable promise for cancer treatment...
Drug discovery strategies: technologies to accelerate translation from target to drugP Workman
Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, Sutton, Surrey SM2 5NG, UK
J Chemother 16:13-5. 2004..Similarly, the drug discovery process is being accelerated by a range of other valuable technologies. Using these approaches, we can expect to see a range of personalised molecular therapeutics becoming available for cancer treatment...
Genomics and the second golden era of cancer drug developmentPaul Workman
Cancer Research UK Centre for Cancer Therapeutics, Sutton, UK
Mol Biosyst 1:17-26. 2005..The second golden era is now underway in which cancer genomics will direct drug development...
Altered states: selectively drugging the Hsp90 cancer chaperonePaul Workman
Cancer Research UK Centre for Cancer Therapeutics, Institute of Cancer Research, Sutton, Surrey, UK
Trends Mol Med 10:47-51. 2004..However, the reason for therapeutic selectivity in cancer cells versus normal cells is unclear. New research now shows that Hsp90 exists in cancer cells in a heightened, activated state that is highly susceptible to inhibition by 17AAG...
Strategies for treating cancers caused by multiple genome abnormalities: from concepts to cures?Paul Workman
Cancer Research UK Centre for Cancer Therapeutics, Institute of Cancer Research, 15 Cotswold Road, Sutton, Surrey, SM2 5NG, UK
Curr Opin Investig Drugs 4:1410-5. 2003..g., imatinib in chronic myeloid leukemia and gastrointestinal stromal tumors. Other multigenic cancers will require drug cocktails or single drugs acting on multiple downstream targets...
Combinatorial attack on multistep oncogenesis by inhibiting the Hsp90 molecular chaperonePaul Workman
Cancer Research UK Centre for Cancer Therapeutics, Institute of Cancer Research, Cotswold Road, Belmont, Sutton, Surrey SM2 5NG, UK
Cancer Lett 206:149-57. 2004..This article reviews the current status and future prospects for the exploitation of Hsp90 as a new molecular target for cancer treatment...
Drugging the cancer chaperone HSP90: combinatorial therapeutic exploitation of oncogene addiction and tumor stressPaul Workman
Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, Haddow Laboratories, 15 Cotswold Road, Sutton, Surrey UK
Ann N Y Acad Sci 1113:202-16. 2007..We stress how basic and translational research has been mutually beneficial and indicate future directions to enhance our understanding of molecular chaperones and their exploitation in cancer and other diseases...
The opportunities and challenges of personalized genome-based molecular therapies for cancer: targets, technologies, and molecular chaperonesPaul Workman
Cancer Research UK Centre for Cancer Therapeutics, Institute of Cancer Research, Sutton, Surrey, SN2 5NG, UK
Cancer Chemother Pharmacol 52:S45-56. 2003....
Minimally invasive pharmacokinetic and pharmacodynamic technologies in hypothesis-testing clinical trials of innovative therapiesPaul Workman
Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, Sutton, Surrey, UK
J Natl Cancer Inst 98:580-98. 2006..Development, validation, and implementation of minimally invasive PK/PD methods are encouraged...
New cancer drugs on the horizonPaul Workman
The Institute of Cancer Research, Cancer Research UK Centre for Cancer Therapeutics, Haddow Laboratories, 15 Cotswold Road, Sutton, Surrey, SM2 5NG, UK
Future Oncol 1:315-8. 2005
Exploiting the cancer genome: strategies for the discovery and clinical development of targeted molecular therapeuticsTimothy A Yap
Cancer Research UK Cancer Therapeutics Unit, Division of Cancer Therapeutics, The Institute of Cancer Research, Haddow Laboratories, Sutton, Surrey SM2 5NG, United Kingdom
Annu Rev Pharmacol Toxicol 52:549-73. 2012....
Resisting targeted therapy: fifty ways to leave your EGFRPaul Workman
Cancer Research UK Cancer Therapeutics Unit, Division of Cancer Therapeutics, The Institute of Cancer Research, Haddow Laboratories, Sutton, UK
Cancer Cell 19:437-40. 2011..Two recent papers have identified novel ways by which non-small cell lung cancers can exhibit resistance to EGFR inhibitors and suggest new therapeutic workarounds...
Probing the probes: fitness factors for small molecule toolsPaul Workman
Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, Sutton, Surrey, SM2 5NG, UK
Chem Biol 17:561-77. 2010....
The clinical applications of heat shock protein inhibitors in cancer - present and futureUdai Banerji
Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, 15 Cotswold Road, Sutton, Surrey SM2 5NG, UK
Curr Cancer Drug Targets 3:385-90. 2003..Examples of likely disease targets include chronic myeloid leukaemia, melanoma, breast, ovarian, brain, thyroid, colorectal and prostate cancer...
Guidelines for the welfare and use of animals in cancer researchP Workman
Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, Cotswold Road, Sutton, Surrey SM2 5NG, UK
Br J Cancer 102:1555-77. 2010....
Unveiling the secrets of the ancestral PI3 kinase Vps34Paul Workman
Cancer Research UK Centre for Cancer Therapeutics and Section of Cancer Therapeutics, The Institute of Cancer Research, Haddow Laboratories, Sutton, Surrey, SM2 5NG, UK
Cancer Cell 17:421-3. 2010..A report in Science unveils the Vps34 structure, providing new insights into the catalytic mechanism, explaining why Vsp34 is so difficult to inhibit, and facilitating design of chemical tools and potential drugs...
Drugging the PI3 kinome: from chemical tools to drugs in the clinicPaul Workman
Cancer Research UK Centre for Cancer Therapeutics, Section of Cancer Therapeutics, The Institute of Cancer Research, Haddow Laboratories, Sutton, Surrey, United Kingdom
Cancer Res 70:2146-57. 2010..The challenges and outlook for drugging the PI3 kinome are discussed in the more general context of the role of structural biology and chemical biology in innovative drug discovery...
Overview: translating Hsp90 biology into Hsp90 drugsPaul Workman
Cancer Research UK Centre for Cancer Therapeutics, Institute of Cancer Research, Sutton, Surrey SM2 5NG UK
Curr Cancer Drug Targets 3:297-300. 2003..The opportunities and challenges involved in translating the fast moving biology of Hsp90 into patient benefit is discussed...
Inhibition of signal transduction by the Hsp90 inhibitor 17-allylamino-17-demethoxygeldanamycin results in cytostasis and apoptosisI Hostein
Cancer Research Campaign Centre for Cancer Therapeutics, Institute of Cancer Research, Sutton, Surrey SM2 5NG, United Kingdom
Cancer Res 61:4003-9. 2001....
Solid-phase immunoassays in mechanism-based drug discovery: their application in the development of inhibitors of the molecular chaperone heat-shock protein 90Anthea Hardcastle
Haddow Laboratories, Cancer Research UK Centre for Cancer Therapeutics at Institute of Cancer Research, Sutton, Surrey, UK
Assay Drug Dev Technol 3:273-85. 2005..Finally, comparison is made between the use and applicability of this type of immunoassay and other techniques such as western blotting, immunohistochemistry, and flow cytometry analysis...
Silencing the cochaperone CDC37 destabilizes kinase clients and sensitizes cancer cells to HSP90 inhibitorsJ R Smith
Signal Transduction and Molecular Pharmacology Team, Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, Haddow Laboratories, Surrey, UK
Oncogene 28:157-69. 2009..These results support an essential role for CDC37 in concert with HSP90 in maintaining oncogenic protein kinase clients and endorse the therapeutic potential of targeting CDC37 in cancer...
Preclinical antitumor activity and pharmacodynamic studies with the farnesyl protein transferase inhibitor R115777 in human breast cancerL R Kelland
CRC Centre for Cancer Therapeutics, The Institute of Cancer Research, Surrey SM2 5NG, United Kingdom
Clin Cancer Res 7:3544-50. 2001....
Inhibition of the heat shock protein 90 molecular chaperone in vitro and in vivo by novel, synthetic, potent resorcinylic pyrazole/isoxazole amide analoguesSwee Y Sharp
Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, Haddow Laboratories, Sutton, Surrey, United Kingdom
Mol Cancer Ther 6:1198-211. 2007..The results indicate the therapeutic potential of the resorcinylic pyrazole/isoxazole amide analogues as HSP90 inhibitors...
In vitro and in vivo pharmacokinetic-pharmacodynamic relationships for the trisubstituted aminopurine cyclin-dependent kinase inhibitors olomoucine, bohemine and CYC202Florence I Raynaud
Cancer Research UK Centre for Cancer Therapeutics at The Institute of Cancer Research, Haddow Laboratories, Belmont, Sutton, United Kingdom
Clin Cancer Res 11:4875-87. 2005..Such markers may be potentially useful in the clinical development of CYC202 and other cyclin-dependent kinase inhibitors...
Identification of 4-(4-aminopiperidin-1-yl)-7H-pyrrolo[2,3-d]pyrimidines as selective inhibitors of protein kinase B through fragment elaborationJohn J Caldwell
Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, 15 Cotswold Road, Sutton, Surrey, U K
J Med Chem 51:2147-57. 2008..Selectivity for PKB vs PKA was observed with 4-aminopiperidine derivatives, and the most PKB-selective inhibitor (30-fold) showed significantly different bound conformations between PKA and PKA-PKB chimera...
Molecular pharmacology of phosphatidylinositol 3-kinase inhibition in human gliomaSandrine Guillard
Signal Transduction and Molecular Pharmacology Team, Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, Sutton, Surrey, UK
Cell Cycle 8:443-53. 2009..Our results support the therapeutic potential for PI3 kinase inhibitors with a PI-103-like profile as therapeutic agents for the treatment of glioma...
The role of functional and molecular imaging in cancer drug discovery and developmentB M Seddon
Cancer Research UK Centre for Cancer Therapeutics, Institute of Cancer Research, Cotswold Road, Sutton, Surrey SM2 5NG, UK
Br J Radiol 76:S128-38. 2003....
A phase I trial of the selective oral cyclin-dependent kinase inhibitor seliciclib (CYC202; R-Roscovitine), administered twice daily for 7 days every 21 daysC Benson
Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, Sutton, Surrey, UK
Br J Cancer 96:29-37. 2007..No objective tumour responses were noted, but disease stabilisation was recorded in eight patients; this lasted for a total of six courses (18 weeks) in a patient with ovarian cancer...
Establishment and characterization of acquired resistance to the farnesyl protein transferase inhibitor R115777 in a human colon cancer cell lineVictoria Smith
CRC Centre for Cancer Therapeutics, The Institute of Cancer Research, Sutton SM2 5NG, UK
Clin Cancer Res 8:2002-9. 2002..The results suggest that the development of clinical resistance may occur with farnesyl protein transferase inhibitors...
Noninvasive magnetic resonance spectroscopic pharmacodynamic markers of the choline kinase inhibitor MN58b in human carcinoma modelsNada M S Al-Saffar
Cancer Research UK Clinical Magnetic Resonance Research Group, The Institute of Cancer Research and The Royal Marsden NHS Foundation Trust, Sutton, Surrey, United Kingdom
Cancer Res 66:427-34. 2006..The decrease in phosphocholine, total choline, and phosphomonoesters may have potential as noninvasive pharmacodynamic biomarkers for determining tumor response following treatment with choline kinase inhibitors...
Benzoquinone ansamycin heat shock protein 90 inhibitors modulate multiple functions required for tumor angiogenesisSharon Sanderson
Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, Surrey, United Kingdom
Mol Cancer Ther 5:522-32. 2006....
Finding the needle in the haystack: why high-throughput screening is good for your healthG Wynne Aherne
Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, 15 Cotswold Road, Sutton, Surrey SM2 5NG, UK
Breast Cancer Res 4:148-54. 2002..Examples of successful drug discovery programmes based on high-throughput screening are described, and these offer potential in the treatment of breast cancer and other malignancies...
ErbB2 overexpression in an ovarian cancer cell line confers sensitivity to the HSP90 inhibitor geldanamycinVicki Smith
CRC Center for Cancer Therapeutics, The Institute of Cancer Research, Sutton, UK
Anticancer Res 22:1993-9. 2002..These data suggest that erbB2 status in ovarian cancr may contribute to chemosensitivity, in some cases leading to increased sensitivity (as with geldanamycin) but in other cases leading to resistance (as with cisplatin)...
The Cyclin-dependent kinase inhibitor CYC202 (R-roscovitine) inhibits retinoblastoma protein phosphorylation, causes loss of Cyclin D1, and activates the mitogen-activated protein kinase pathwaySteven R Whittaker
Cancer Research UK Centre for Cancer Therapeutics, Institute of Cancer Research, Haddow Laboratories, Sutton, Surrey SM2 5NG, United Kingdom
Cancer Res 64:262-72. 2004..In addition to providing useful insights into the molecular pharmacology of CYC202 in human cancer cells, the results also suggest potential pharmacodynamic end points for use in clinical trials with the drug...
Isothiazolones as inhibitors of PCAF and p300 histone acetyltransferase activityLindsay Stimson
Cancer Research UK Centre for Cancer Therapeutics at The Institute of Cancer Research, Haddow Laboratories, 15 Cotswold Road, Sutton, Surrey SM2 5NG, United Kingdom
Mol Cancer Ther 4:1521-32. 2005..As one of the first series of small-molecule inhibitors of HAT activity, further analogue synthesis is being pursued to examine the potential scope for reducing chemical reactivity while maintaining HAT inhibition...
New drug targets for genomic cancer therapy: successes, limitations, opportunities and future challengesP Workman
CRC Centre for Cancer Therapeutics, Institute for Cancer Research, Sutton, Surrey, SN2 5NG, UK
Curr Cancer Drug Targets 1:33-47. 2001..However, many challenges remain as we test the vision of individualised combinatorial genome-based therapy, using drugs targeted to every significant molecular abnormality in cancer...
Establishment of an isogenic human colon tumor model for NQO1 gene expression: application to investigate the role of DT-diaphorase in bioreductive drug activation in vitro and in vivoS Y Sharp
CRC Centre for Cancer Therapeutics, The Institute of Cancer Research, Sutton, Surrey, United Kingdom
Mol Pharmacol 58:1146-55. 2000..This isogenic model should be valuable for mechanistic studies and bioreductive drug development...
Pharmacokinetic-pharmacodynamic relationships for the heat shock protein 90 molecular chaperone inhibitor 17-allylamino, 17-demethoxygeldanamycin in human ovarian cancer xenograft modelsUdai Banerji
Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, Haddow Laboratories, Sutton, United Kingdom
Clin Cancer Res 11:7023-32. 2005..CONCLUSION: Pharmacokinetic-pharmacodynamic relationships were established for 17-AAG. This information formed the basis of a pharmacokinetic-pharmacodynamic-driven phase I trial...
Mechanism of action of the Aurora kinase inhibitor CCT129202 and in vivo quantification of biological activityFlorence Chan
Centre for Cancer Therapeutics, The Institute of Cancer Research, Cancer Research UK, 15 Cotswold Road, Belmont, Surrey SM2 5NG, United Kingdom
Mol Cancer Ther 6:3147-57. 2007..This has facilitated the use of 3'-deoxy-3'[(18)F]fluorothymidine-positron emission tomography to measure noninvasively the biological activity of the Aurora kinase inhibitor CCT129202 in vivo...
An in vitro and in vivo study of the combination of the heat shock protein inhibitor 17-allylamino-17-demethoxygeldanamycin and carboplatin in human ovarian cancer modelsUdai Banerji
Cancer Research UK Centre for Cancer Therapeutics, Haddow Laboratories, The Institute of Cancer Research, 15 Cotswold Road, Sutton, Surrey SM2 5NG, UK
Cancer Chemother Pharmacol 62:769-78. 2008..To study the interactions of the heat shock protein 90 (HSP90) inhibitor 17-allylamino-17-demethoxygeldanamycin (17-AAG) and carboplatin in vitro and in vivo...
Histone modification enzymes: novel targets for cancer drugsRebecca Kristeleit
Cancer Research UK Centre for Cancer Therapeutics, Institute of Cancer Research, Sutton, Surrey, UK
Expert Opin Emerg Drugs 9:135-54. 2004..It is likely that many of the enzymes involved in the control of histone modification will provide therapeutic opportunities for the treatment of cancer, including histone methyltransferases and Aurora kinases...
Characterization of a human colorectal carcinoma cell line with acquired resistance to flavopiridolV Smith
CRC Centre for Cancer Therapeutics, The Institute of Cancer Research, Sutton, Surrey, United Kingdom
Mol Pharmacol 60:885-93. 2001..However, transfection of cyclin E to increase expression of this protein did not result in an increase in resistance to flavopiridol. Thus, up-regulation of cyclin E alone does not seem to cause resistance to this cdk inhibitor...
Drugging the cancer kinome: progress and challenges in developing personalized molecular cancer therapeuticsP Workman
Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, Haddow Laboratories, Sutton, Surrey
Cold Spring Harb Symp Quant Biol 70:499-515. 2005..Three projects in which the author's laboratory is involved are reviewed in detail. These involve the discovery and development of inhibitors of cyclin-dependent kinases, phosphoinositide 3-kinases, and the Hsp90 molecular chaperone...
Inhibitors of the HSP90 molecular chaperone: current statusSwee Sharp
Signal Transduction and Molecular Pharmacology Team, Cancer Research UK, Centre for Cancer Therapeutics, The Institute of Cancer Research, Haddow Laboratories, Sutton, Surrey, SM2 5NG, United Kingdom
Adv Cancer Res 95:323-48. 2006..Thus, it is not surprising that new HSP90 agents are under development against this novel target for cancer therapy and several show promise...
Silencing of HSP90 cochaperone AHA1 expression decreases client protein activation and increases cellular sensitivity to the HSP90 inhibitor 17-allylamino-17-demethoxygeldanamycinJoanna L Holmes
Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, Haddow Laboratories, Sutton, Surrey, United Kingdom
Cancer Res 68:1188-97. 2008..Furthermore, AHA1 knockdown could sensitize cancer cells to 17-AAG. We conclude that modulation of AHA1 might be a potential therapeutic strategy to increase sensitivity to HSP90 inhibitors...
A duplexed phenotypic screen for the simultaneous detection of inhibitors of the molecular chaperone heat shock protein 90 and modulators of cellular acetylationAnthea Hardcastle
Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, Haddow Laboratories, 15 Cotswold Road, Sutton, Surrey SM2 5NG, United Kingdom
Mol Cancer Ther 6:1112-22. 2007..In the acetylation arm, two compounds increased cellular acetylation, one of which inhibited histone deacetylase activity. A third compound decreased cellular histone acetylation, potentially through a novel mechanism of action...
Mechanistic evaluation of the novel HSP90 inhibitor NVP-AUY922 in adult and pediatric glioblastomaNathalie Gaspar
Signal Transduction and Molecular Pharmacology Team, Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, Sutton, Surrey, UK
Mol Cancer Ther 9:1219-33. 2010....
The identification, synthesis, protein crystal structure and in vitro biochemical evaluation of a new 3,4-diarylpyrazole class of Hsp90 inhibitorsKwai-Ming J Cheung
Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, Cancer Research UK and Haddow Laboratories, 15 Cotswold Road, Sutton, Surrey SM2 5NG, UK
Bioorg Med Chem Lett 15:3338-43. 2005..Some initial structure-activity relationships are discussed, as well as the crystal structure of CCT018159 bound to Hsp90...
Gene and protein expression profiling of human ovarian cancer cells treated with the heat shock protein 90 inhibitor 17-allylamino-17-demethoxygeldanamycinAlison Maloney
Haddow Laboratories, Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, Surrey, United Kingdom
Cancer Res 67:3239-53. 2007....
Inhibition of Kirsten-ras expression in human colorectal cancer using rationally selected Kirsten-ras antisense oligonucleotidesP J Ross
Cancer Research Campaign Centre for Cancer Therapeutics, Institute of Cancer Research, 15 Cotswold Road, Sutton, Surrey, SM2 5NG, United Kingdom
Mol Cancer Ther 1:29-41. 2001....
The impact of genomic and proteomic technologies on the development of new cancer drugsP Workman
Cancer Research UK, Centre for Cancer Therapeutics, Institute of Cancer Research, Sutton, UK
Ann Oncol 13:115-24. 2002
Challenges of PK/PD measurements in modern drug developmentP Workman
Cancer Research UK Centre for Cancer Therapeutics, Institute of Cancer Research, Sutton, Surrey SN2 5NG, UK
Eur J Cancer 38:2189-93. 2002
Preclinical pharmacokinetics and metabolism of a novel prototype DNA-PK inhibitor NU7026B P Nutley
Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, Haddow Laboratories, 15 Cotswold Road, Sutton SM2 5NG, UK
Br J Cancer 93:1011-8. 2005..The pharmacokinetic simulations performed predict that NU7026 will have to be administered four times per day at 100 mg kg(-1) i.p. in order to obtain the drug exposure required for radiosensitisation...
Gene expression microarray analysis in cancer biology, pharmacology, and drug development: progress and potentialP A Clarke
Cancer Research Campaign Centre for Cancer Therapeutics, E Block, Institute of Cancer Research, 15 Cotswold Road, SM2 5NG, Sutton, Surrey, UK
Biochem Pharmacol 62:1311-36. 2001..As a result of further technological improvements and decreasing costs, the use of microarrays will become an essential and potentially routine tool for cancer and biomedical research...
Dual targeting of HSC70 and HSP72 inhibits HSP90 function and induces tumor-specific apoptosisMarissa V Powers
Signal Transduction and Molecular Pharmacology Team, Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, Haddow Laboratories, Sutton, Surrey SM2 5NG, UK
Cancer Cell 14:250-62. 2008..Importantly, simultaneous silencing of HSP70 isoforms in nontumorigenic cell lines does not result in comparable growth arrest or induction of apoptosis, indicating a potential therapeutic window...
In vitro biological characterization of a novel, synthetic diaryl pyrazole resorcinol class of heat shock protein 90 inhibitorsSwee Y Sharp
Haddow Laboratories, Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, 15 Cotswold Road, Belmont, Sutton, Surrey, UK
Cancer Res 67:2206-16. 2007..g., aqueous solubility, independence from NQO1 and P-glycoprotein). These compounds form the basis for further structure-based optimization to identify more potent inhibitors suitable for clinical development...
The contemporary drug development process: advances and challenges in preclinical and clinical developmentMichelle D Garrett
Cancer Research UK Centre for Cancer Therapeutics, Institute of Cancer Research, 15 Cotswold Road, Sutton, Surrey, SM2 5NG, U.K
Prog Cell Cycle Res 5:145-58. 2003..However, many of the concepts, approaches and issues are generally common to other therapeutic areas...
Potentiation of paclitaxel activity by the HSP90 inhibitor 17-allylamino-17-demethoxygeldanamycin in human ovarian carcinoma cell lines with high levels of activated AKTNivedita Sain
The Haddow Laboratories, The Institute of Cancer Research, 15 Cotswold Road, Sutton, Surrey SM2 5NG, United Kingdom
Mol Cancer Ther 5:1197-208. 2006..In conclusion, 17-AAG may sensitize a subset of ovarian cancer to paclitaxel, particularly those tumors in which resistance is driven by ERBB2 and/or p-AKT...
The cyclin-dependent kinase inhibitor seliciclib (R-roscovitine; CYC202) decreases the expression of mitotic control genes and prevents entry into mitosisSteven R Whittaker
Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, Haddow Laboratories, Sutton, Surrey, UK
Cell Cycle 6:3114-31. 2007....
Application of meso scale technology for the measurement of phosphoproteins in human tumor xenograftsSharon M Gowan
Cancer Research UK Centre for Cancer Therapeutics, Institute of Cancer Research, Sutton, Surrey, UK
Assay Drug Dev Technol 5:391-401. 2007..This optimized procedure can be used for both in vitro and in vivo analysis, unlike an established fixed-cell ELISA with a time-resolved fluorescent end point...
Clinical anticancer drug development: targeting the cyclin-dependent kinasesC Benson
Section of Medicine and Cancer Research UK Centre for Cancer Therapeutics, Institute for Cancer Research and Royal Marsden Hospital, Downs Road, Sutton, Surrey SM2 5PT, UK
Br J Cancer 92:7-12. 2005..The critical nature of these proteins in cell cycling raises hope that targeting them may result in selective cytotoxicity and valuable anticancer activity...
Evaluation of the cassette dosing approach for assessing the pharmacokinetics of geldanamycin analogues in miceN F Smith
Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, 15 Cotswold Road, SM2 5NG, Sutton, UK
Cancer Chemother Pharmacol 54:475-86. 2004..The issues identified for this compound series should also be considered in assessing the suitability of cassette dosing for other chemotypes...
A phase I study of the nitroimidazole hypoxia marker SR4554 using 19F magnetic resonance spectroscopyC P Lee
Cancer Research UK Centre for Cancer Therapeutics, Cancer Research UK Clinical Magnetic Resonance Research Group and Section of Medicine, The Institute of Cancer Research and Drug Development Unit, The Royal Marsden Hospital, 15 Cotswold Road, Sutton, Surrey SM2 5NG, UK
Br J Cancer 101:1860-8. 2009..Preliminary MRS studies demonstrated some evidence of 19F retention in tumour. In this study we investigated higher doses of SR4554 and intratumoral localisation of the 19F MRS signal...
Molecular and phenotypic characterisation of paediatric glioma cell lines as models for preclinical drug developmentDorine A Bax
Paediatric Oncology, The Institute of Cancer Research, Sutton, United Kingdom
PLoS ONE 4:e5209. 2009..We have carried out a detailed molecular and phenotypic characterisation of a series of paediatric high grade glioma cell lines in comparison to routinely used adult lines...
EGFRvIII deletion mutations in pediatric high-grade glioma and response to targeted therapy in pediatric glioma cell linesDorine A Bax
Paediatric Oncology, The Institute of Cancer Research, Sutton, United Kingdom
Clin Cancer Res 15:5753-61. 2009..EGFR has thus far been considered to play a less important role in pediatric glioma, although extensive data are lacking. We have sought to clarify the role of EGFR in pediatric high-grade glioma (HGG)...
The identification of novel PLC-gamma inhibitors using virtual high throughput screeningJóhannes Reynisson
Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, 15 Cotswold Road, Sutton, Surrey SM2 5NG, UK
Bioorg Med Chem 17:3169-76. 2009..This translated into approximately 15 microM in a functional assay in cells. About 30% of the virtual hits showed activity against PLC-gamma (IC(50)<50 microM)...
Acquired resistance to 17-allylamino-17-demethoxygeldanamycin (17-AAG, tanespimycin) in glioblastoma cellsNathalie Gaspar
Signal Transduction and Molecular Pharmacology Team, Cancer Research UK Centre for Cancer The Institute of Cancer Research, Sutton, Surrey SM2 5NG, United Kingdom
Cancer Res 69:1966-75. 2009..In conclusion, low NQO1 activity is a likely mechanism of acquired resistance to 17-AAG in GB, melanoma, and, possibly, other tumor types. Such resistance can be overcome with novel HSP90 inhibitors...
Targeting the PI3K-AKT-mTOR pathway: progress, pitfalls, and promisesTimothy A Yap
Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, 15 Cotswold Road, Sutton, Surrey, UK
Curr Opin Pharmacol 8:393-412. 2008..Finally, we discuss the issues and limitations involved with targeting the PI3K-AKT-mTOR pathway, and predict what the future may hold for these novel anticancer therapeutics...
Validation of the fluorinated 2-nitroimidazole SR-4554 as a noninvasive hypoxia marker detected by magnetic resonance spectroscopyBeatrice M Seddon
Cancer Research UK Centre for Cancer Therapeutics, Institute of Cancer Research, Sutton, Surrey SM2 5NG, United Kingdom
Clin Cancer Res 8:2323-35. 2002..These findings support the use of SR 4554 as a noninvasive hypoxia marker...
Targeting of multiple signalling pathways by heat shock protein 90 molecular chaperone inhibitorsMarissa V Powers
Signal Transduction and Molecular Pharmacology Team, Cancer Research UK Centre for Cancer Therapeutics, Haddow Laboratories, The Institute of Cancer Research, Sutton, Surrey SM2 5NG, UK
Endocr Relat Cancer 13:S125-35. 2006..We also review the discovery and development of novel small-molecule inhibitors and discuss alternative approaches to inhibit HSP90 activity, both of which offer exciting prospects for the future...
Gene expression microarray technologies in the development of new therapeutic agentsPaul A Clarke
Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, Haddow Laboratories, Sutton, Surrey SM2 5NG, UK
Eur J Cancer 40:2560-91. 2004..The approach can identify on-target and off-target effects. It can be used to identify molecular biomarkers for proof of concept studies, pharmacodynamic endpoints and prognostic markers for predicting outcome and patient selection...
Phase I pharmacokinetic and pharmacodynamic study of 17-allylamino, 17-demethoxygeldanamycin in patients with advanced malignanciesUdai Banerji
Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, 15 Cotswold Rd, Sutton, Surrey SM2 5NG, UK
J Clin Oncol 23:4152-61. 2005..We recommend this dose for phase II clinical trials...
Identification of novel small molecule inhibitors of hypoxia-inducible factor-1 that differentially block hypoxia-inducible factor-1 activity and hypoxia-inducible factor-1alpha induction in response to hypoxic stress and growth factorsNoan-Minh Chau
Cell Growth Regulation and Angiogenesis Team, Cancer Research UK Centre for Cancer Therapeutics, Institute of Cancer Research, Sutton, Surrey, United Kingdom
Cancer Res 65:4918-28. 2005..Our cell-based assay approach has successfully identified novel compounds that differentially target hypoxia and/or growth factor-mediated induction of HIF-1alpha...
Selective FLT3 inhibition of FLT3-ITD+ acute myeloid leukaemia resulting in secondary D835Y mutation: a model for emerging clinical resistance patternsA S Moore
Cancer Research UK Cancer Therapeutics Unit, Division of Cancer Therapeutics, The Institute of Cancer Research, Sutton, UK
Leukemia 26:1462-70. 2012....
A chemical inhibitor of PPM1D that selectively kills cells overexpressing PPM1DS Rayter
Breakthrough Breast Cancer Research Centre, The Institute of Cancer Research, London, UK
Oncogene 27:1036-44. 2008..These results further validate PPM1D as a therapeutic target and identify a proof-of-principle small molecule inhibitor...
Magnetic resonance spectroscopy monitoring of mitogen-activated protein kinase signaling inhibitionMounia Beloueche-Babari
Cancer Research UK Clinical Magnetic Resonance Research Group, Institute of Cancer Research and Royal Marsden NHS Foundation Trust, Downs Road, Sutton, Surrey SM2 5PT, UK
Cancer Res 65:3356-63. 2005..Thus, phosphocholine has potential as a noninvasive pharmacodynamic marker for monitoring MAPK signaling blockade...
The application of cassette dosing for pharmacokinetic screening in small-molecule cancer drug discoveryNicola F Smith
Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, Haddow Laboratories, 15 Cotswold Road, Sutton, Surrey SM2 5NG, United Kingdom
Mol Cancer Ther 6:428-40. 2007..Subject to critical analysis and validation in each case, the use of cassette dosing is recommended in appropriate chemical series to enhance the efficiency of drug discovery and reduce animal usage...
Inhibitors of the HSP90 molecular chaperone: attacking the master regulator in cancerEdward McDonald
Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, Haddow Laboratories, 15 Cotswold Road, Belmont, Sutton, Surrey SM2 5NG, UK
Curr Top Med Chem 6:1091-107. 2006..Medicinal chemists have used this information to develop high affinity lead compounds. Recent research provides the platform for exciting developments in the area of HSP90 inhibition over the next few years...
Assays for the identification and evaluation of histone acetyltransferase inhibitorsG Wynne Aherne
Cancer Research UK Centre for Cancer Therapeutics, Institute of Cancer Research, 15 Cotswold Road, Sutton, SM2 5NG, UK
Methods 26:245-53. 2002..This latter method provides a medium-throughput alternative to the use of immunoblotting for mechanistic studies...
Structure-based design of molecular cancer therapeuticsRob L M Van Montfort
Section of Structural Biology, The Institute of Cancer Research, Chester Beatty Laboratories, Chelsea, London SW3 6JB, UK
Trends Biotechnol 27:315-28. 2009..Structure-based design approaches have also been successfully applied to the protein-protein interaction targets p53-MDM2 and the Bcl-2 family...
An evaluation of the ability of pifithrin-alpha and -beta to inhibit p53 function in two wild-type p53 human tumor cell linesMike I Walton
Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, Haddow Laboratories, Sutton Surrey
Mol Cancer Ther 4:1369-77. 2005..In conclusion, neither PFT-alpha nor -beta can be regarded as a ubiquitous inhibitor of p53 function, and caution should be exercised in the use of these agents as specific p53 inhibitors...
Pharmacologic characterization of a potent inhibitor of class I phosphatidylinositide 3-kinasesFlorence I Raynaud
Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, Haddow and McElwain Laboratories, Sutton, Surrey, United Kingdom
Cancer Res 67:5840-50. 2007....
A liquid chromatographic-tandem mass spectrometric method for the determination of two selective thymidylate synthase inhibitors, BGC945 and BGC638, in mouse plasmaNadya Wood
Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, 15 Cotswold Road, Sutton, Surrey SM2 5NG, UK
J Chromatogr B Analyt Technol Biomed Life Sci 824:181-8. 2005..This validated method has been used to define the first pharmacokinetic report of BGC945 and BGC638 in mice...
Hit generation and exploration: imidazo[4,5-b]pyridine derivatives as inhibitors of Aurora kinasesVassilios Bavetsias
Department of Chemistry, Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, Cancer Research UK Laboratory, 15 Cotswold Road, Sutton, Surrey SM2 5NG, UK
Bioorg Med Chem Lett 17:6567-71. 2007..042, 0.198 and 0.227microM, respectively. Compound 31 inhibits cell proliferation and has good microsomal stability...
AHR- and DNA-damage-mediated gene expression responses induced by benzo(a)pyrene in human cell linesSarah L Hockley
Section of Molecular Carcinogenesis, The Institute of Cancer Research, Surrey, UK
Chem Res Toxicol 20:1797-810. 2007..Promoter analysis identified candidate genes for direct transcriptional regulation by either AHR or p53. These analyses have further dissected and characterized the complex cellular response to BaP...
Modulation of melanoma cell phospholipid metabolism in response to heat shock protein 90 inhibitionMounia Beloueche-Babari
Cancer Research UK and EPSRC Cancer Imaging Centre, The Institute of Cancer Research and The Royal Marsden NHS Foundation Trust, Sutton, Surrey, United Kingdom
Oncotarget 1:185-97. 2010..Our findings provide a basis for using metabolic changes as non-invasive indicators of Hsp90 inhibition and potentially as biomarkers of anticancer activity with Hsp90 drugs in malignant melanoma and possibly in other cancers...
Scoring a bull's-eye against cancer genome targetsP Workman
CRC Centre for Cancer Therapeutics, Institute of Cancer Research, Sutton, Surrey, UK
Curr Opin Pharmacol 1:342-52. 2001..Drugs acting on a range of new genome-based molecular targets are now in preclinical and clinical development...
Characterisation of molecular events following cisplatin treatment of two curable ovarian cancer models: contrasting role for p53 induction and apoptosis in vivoP A Clarke
Cancer Research UK Centre for Cancer Therapeutics, Institute of Cancer Research, Haddow Laboratories, Sutton, Surrey SM2 5NG, UK
Br J Cancer 91:1614-23. 2004..Our observations show that a marked in vivo response to cisplatin can occur via p53-dependent apoptosis or independently of p53 status in human ovarian xenografts...
The assessment of antiangiogenic and antivascular therapies in early-stage clinical trials using magnetic resonance imaging: issues and recommendationsM O Leach
Cancer Research UK Clinical Magnetic Resonance Research Group, Institute of Cancer Research, Royal Marsden Hospital, Sutton, Surrey, UK
Br J Cancer 92:1599-610. 2005..This publication reports the outcome of a workshop that considered the methodology and design of magnetic resonance studies, recommending how this new tool might best be used...
From darkness to light with biomarkers in early clinical trials of cancer drugsC P Carden
The Royal Marsden National Health Service Foundation Trust, Sutton, Surrey, UK
Clin Pharmacol Ther 85:131-3. 2009....
Measurement of tumor oxygenation: in vivo comparison of a luminescence fiber-optic sensor and a polarographic electrode in the p22 tumorB M Seddon
CRC Centre for Cancer Therapeutics, Institute of Cancer Research, 15 Cotswold Road, Sutton, Surrey, SM2 5PT, United Kingdom
Radiat Res 155:837-46. 2001..6 to 28.1 mm hg...
A Phase I study of the angiogenesis inhibitor SU5416 (semaxanib) in solid tumours, incorporating dynamic contrast MR pharmacodynamic end pointsA O'DONNELL
Institute of Cancer Research, and Royal Marsden Hospital, Sutton SM2 5PT, UK
Br J Cancer 93:876-83. 2005..The 145 mg m-2 dose level is thus the recommended dose for future study...
High-throughput screening for identification of small molecule inhibitors of histone acetyltransferases using scintillating microplates (FlashPlate)F Turlais
CRC Centre for Cancer Therapeutics, Institute of Cancer Research, Sutton, Surrey, SM2 5NG, United Kingdom
Anal Biochem 298:62-8. 2001..This resulted in a simple "mix and measure" assay that is currently being used for the identification of HAT inhibitors...
Use of pharmacokinetic/pharmacodynamic biomarkers to support rational cancer drug developmentDebashis Sarker
Signal Transduction and Molecular Pharmacology Team, Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, Haddow Laboratories, Sutton, Surrey, SM2 5NG, UK
Biomark Med 1:399-417. 2007..The challenges of performing assays to satisfy regulatory requirements have been the subject of much debate. We recommend the implementation of appropriate, fit-for-purpose biomarkers in clinical trials of all new cancer drugs...
4-Amino derivatives of the Hsp90 inhibitor CCT018159Xavier Barril
Vernalis Ltd, Granta Park, Cambridge CB1 6GB, UK
Bioorg Med Chem Lett 16:2543-8. 2006....
Novel, potent small-molecule inhibitors of the molecular chaperone Hsp90 discovered through structure-based designBrian W Dymock
Vernalis Ltd, Granta Park, Great Abington, Cambridge, UK
J Med Chem 48:4212-5. 2005..These exhibit enhanced potency against the target in binding and functional assays with accompanying appropriate cellular pharmacodynamic changes. Compound 11 (VER-49009) compares favorably with the clinically evaluated 17-AAG...
Magnetic resonance spectroscopic pharmacodynamic markers of the heat shock protein 90 inhibitor 17-allylamino,17-demethoxygeldanamycin (17AAG) in human colon cancer modelsYuen Li Chung
Cancer Research UK Biomedical Magnetic Resonance Research Group, Department of Basic Medical Sciences, St George s Hospital Medical School, London, UK
J Natl Cancer Inst 95:1624-33. 2003..We used phosphorus magnetic resonance spectroscopy (31P-MRS) to determine whether 17AAG treatment leads to alterations in phospholipids that could serve as pharmacodynamic markers for tumor response to 17AAG...
Inhibition of Hsp90 with synthetic macrolactones: synthesis and structural and biological evaluation of ring and conformational analogs of radicicolNicolas Proisy
School of Chemistry, University of Nottingham, University Park, Nottingham, NG7 2RD, United Kingdom
Chem Biol 13:1203-15. 2006..e., depletion of client proteins with upregulation of Hsp70...
4,5-diarylisoxazole Hsp90 chaperone inhibitors: potential therapeutic agents for the treatment of cancerPaul A Brough
Vernalis Ltd, Granta Park, Great Abington, Cambridge CB21 6GB, U K p brough vernalis com
J Med Chem 51:196-218. 2008..Compound 40f is retained in tumors in vivo when administered i.p., as evaluated by cassette dosing in tumor-bearing mice. In a human colon cancer xenograft model, 40f inhibits tumor growth by approximately 50%...
