I Wilmut

Summary

Affiliation: Institute for Animal Health
Country: UK

Publications

  1. ncbi Somatic cell nuclear transfer
    Ian Wilmut
    Roslin Institute, Roslin, Midlothian, EH25 9PS, UK
    Oncol Res 13:303-7. 2003
  2. ncbi Viable offspring derived from fetal and adult mammalian cells
    I Wilmut
    Roslin Institute Edinburgh, Roslin, Midlothian, UK
    Nature 385:810-3. 1997
  3. ncbi Embryonic and somatic cell cloning
    I Wilmut
    Roslin Institute, Midlothian, UK
    Reprod Fertil Dev 10:639-43. 1998
  4. ncbi New opportunities in animal breeding and production - an introductory remark
    I Wilmut
    Roslin Institute, Roslin, Midlothian, UK
    Anim Reprod Sci 60:5-14. 2000
  5. ncbi Somatic cell nuclear transfer
    I Wilmut
    Roslin Institute, Roslin, Midlothian EH25 9PS, UK
    Nature 419:583-6. 2002
  6. ncbi Modifications to improve the efficiency of zona-free mouse nuclear transfer
    R Ribas
    Department of Gene Function and Development, Roslin Institute, Roslin, Midlothian, Scotland, United Kingdom
    Cloning Stem Cells 8:10-5. 2006
  7. ncbi Deletion of the alpha(1,3)galactosyl transferase (GGTA1) gene and the prion protein (PrP) gene in sheep
    C Denning
    Department of Gene Expression and Development, Roslin Institute, Roslin, Midlothian EH25 9PS, United Kingdom
    Nat Biotechnol 19:559-62. 2001
  8. ncbi Cloned mice derived from embryonic stem cell karyoplasts and activated cytoplasts prepared by induced enucleation
    B Gasparrini
    Division of Gene Expression and Development, Roslin Institute, Roslin, Midlothian EH25 9PS, United Kingdom
    Biol Reprod 68:1259-66. 2003
  9. ncbi Embryo development and establishment of pregnancy after embryo transfer in pigs: coping with limitations in the availability of viable embryos
    T J King
    Department of Gene Expression and Development, Roslin Institute Edinburgh, Midlothian, EH25 9PS, UK
    Reproduction 123:507-15. 2002
  10. ncbi Epigenetic change in IGF2R is associated with fetal overgrowth after sheep embryo culture
    L E Young
    Roslin Institute Edinburgh, Roslin, Midlothian, Scotland, UK
    Nat Genet 27:153-4. 2001

Collaborators

Detail Information

Publications60

  1. ncbi Somatic cell nuclear transfer
    Ian Wilmut
    Roslin Institute, Roslin, Midlothian, EH25 9PS, UK
    Oncol Res 13:303-7. 2003
    ..Improvements in the efficiency may depend upon understanding those mechanisms in the early embryo that establish the precise chromatin structure that governs development...
  2. ncbi Viable offspring derived from fetal and adult mammalian cells
    I Wilmut
    Roslin Institute Edinburgh, Roslin, Midlothian, UK
    Nature 385:810-3. 1997
    ....
  3. ncbi Embryonic and somatic cell cloning
    I Wilmut
    Roslin Institute, Midlothian, UK
    Reprod Fertil Dev 10:639-43. 1998
    ..Finally, cells capable of differentiation into any of the tissues of a patient will provide treatment for diseases reflecting damage to a specific cell population that neither repairs nor replaces itself...
  4. ncbi New opportunities in animal breeding and production - an introductory remark
    I Wilmut
    Roslin Institute, Roslin, Midlothian, UK
    Anim Reprod Sci 60:5-14. 2000
    ..When looking to the future, delegates to this meeting should take pride in what science continues to contribute...
  5. ncbi Somatic cell nuclear transfer
    I Wilmut
    Roslin Institute, Roslin, Midlothian EH25 9PS, UK
    Nature 419:583-6. 2002
    ..When a nucleus is placed in oocyte cytoplasm, the changes in chromatin structure that govern differentiation can be reversed, and the nucleus can be made to control development to term...
  6. ncbi Modifications to improve the efficiency of zona-free mouse nuclear transfer
    R Ribas
    Department of Gene Function and Development, Roslin Institute, Roslin, Midlothian, Scotland, United Kingdom
    Cloning Stem Cells 8:10-5. 2006
    ..5-fold increase) when compared with the results before the modifications. A 2.4-fold increase in overall efficiency was achieved from the oocyte to morula/blastocyst stages...
  7. ncbi Deletion of the alpha(1,3)galactosyl transferase (GGTA1) gene and the prion protein (PrP) gene in sheep
    C Denning
    Department of Gene Expression and Development, Roslin Institute, Roslin, Midlothian EH25 9PS, United Kingdom
    Nat Biotechnol 19:559-62. 2001
    ..Although three of these perished soon after birth, one survived for 12 days. These data show that lambs carrying targeted gene deletions can be generated by nuclear transfer...
  8. ncbi Cloned mice derived from embryonic stem cell karyoplasts and activated cytoplasts prepared by induced enucleation
    B Gasparrini
    Division of Gene Expression and Development, Roslin Institute, Roslin, Midlothian EH25 9PS, United Kingdom
    Biol Reprod 68:1259-66. 2003
    ..Live offspring were derived from both oocyte strains using the latter, whereas a single living mouse was cloned from activated B6CBAF1 cytoplasts...
  9. ncbi Embryo development and establishment of pregnancy after embryo transfer in pigs: coping with limitations in the availability of viable embryos
    T J King
    Department of Gene Expression and Development, Roslin Institute Edinburgh, Midlothian, EH25 9PS, UK
    Reproduction 123:507-15. 2002
    ..Lastly, the present study shows for the first time a comparably effective but novel alternative for pregnancy maintenance in the pig involving the co-transfer of parthenote embryos...
  10. ncbi Epigenetic change in IGF2R is associated with fetal overgrowth after sheep embryo culture
    L E Young
    Roslin Institute Edinburgh, Roslin, Midlothian, Scotland, UK
    Nat Genet 27:153-4. 2001
    ..This highlights the potential benefits of epigenetic diagnostic screening in developing embryo procedures...
  11. ncbi Growth and antrum formation of bovine preantral follicles in long-term culture in vitro
    C G Gutierrez
    Roslin Institute Edinburgh, Roslin, Midlothian EH25 9PS, United Kingdom
    Biol Reprod 62:1322-8. 2000
    ..This culture system may provide a valuable approach for studying the regulation of early follicular development and for production of oocytes for nuclear/embryo transfer, but further work is required...
  12. ncbi Evaluation of gestational deficiencies in cloned sheep fetuses and placentae
    P A De Sousa
    Division of Gene Expression and Development, Roslin Institute, Roslin EH25 9PS, United Kingdom
    Biol Reprod 65:23-30. 2001
    ..These deficiencies are not linked to gross defects in chromosome number...
  13. ncbi Telomerase-immortalized sheep fibroblasts can be reprogrammed by nuclear transfer to undergo early development
    Wei Cui
    Department of Gene Expression and Development, Roslin Institute, Roslin, Midlothian EH25 9PS, United Kingdom
    Biol Reprod 69:15-21. 2003
    ..No fetuses survived beyond 40 days of development, however, showing that although these cells could be substantially reprogrammed, they were not fully competent for nuclear transfer...
  14. ncbi Germinal vesicle material is essential for nucleus remodeling after nuclear transfer
    Shaorong Gao
    Department of Gene Expression and Development, Roslin Institute, Roslin, Midlothian EH25 9PS, Scotland, United Kingdom
    Biol Reprod 67:928-34. 2002
    ..3% of transferred embryos developed to term. These results indicate that GV material is essential for nucleus remodeling after nuclear transfer...
  15. ncbi Proliferative lifespan is conserved after nuclear transfer
    A John Clark
    Department of Gene Expression and Development, Roslin Institute, Roslin, Midlothian EH25 9PS, UK
    Nat Cell Biol 5:535-8. 2003
    ..The rederived lines had the same proliferative capacity and rate of telomere shortening as the donor cell lines, suggesting that these are innate, genetically determined, properties that are conserved by nuclear transfer...
  16. ncbi Live lambs born from zona-pellucida denuded embryos
    William A Ritchie
    Department of Gene Function and Development, Roslin Institute, Roslin, Midlothian, United Kingdom
    Cloning Stem Cells 7:178-82. 2005
    ..In this report, we demonstrate that live lambs can be produced from zona-free IVF embryos. We are pursuing this method as a prerequisite to developing viral vector co-culture delivery strategies...
  17. ncbi Development of a zona-free method of nuclear transfer in the mouse
    R Ribas
    Department of Gene Function and Development, Roslin Institute, Roslin, Midlothian EH25 9PS, Scotland, UK
    Cloning Stem Cells 7:126-38. 2005
    ..0% of transferred embryos developed to term). These observations establish that a zona-free cloning approach is possible in the mouse, although further research is required to increase the efficiency...
  18. ncbi Are there any normal clones?
    Ian Wilmut
    Department of Gene Function and Development, Roslin Institute, Roslin, Midlothian, UK
    Methods Mol Biol 348:307-18. 2006
    ..Because controlled hypothesis testing of clone fidelity or clone health may not be possible, meticulous documentation of such observational evidence is a valuable contribution to the field...
  19. doi Cloning and expression of sheep DNA methyltransferase 1 and its development-specific isoform
    Jane Taylor
    Division of Gene Function and Development, Roslin Institute, Roslin, Midlothian, Edinburgh, UK
    Mol Reprod Dev 76:501-13. 2009
    ..RNAi-mediated knockdown of this novel transcript resulted in embryonic developmental arrest at the late morula stage, suggesting an essential role for this isoform in sheep blastocyst formation...
  20. ncbi Somatic cell nuclear transfer in the pig: control of pronuclear formation and integration with improved methods for activation and maintenance of pregnancy
    Paul A De Sousa
    Department of Gene Expression and Development, Roslin Institute, Roslin, Midlothian EH25 9PS, United Kingdom
    Biol Reprod 66:642-50. 2002
    ..However, no improvement in blastocyst development was observed. Using fetal fibroblasts as nuclear donor cells, a live cloned piglet was produced in a pregnancy that was maintained by cotransfer of parthenogenetic embryos...
  21. ncbi Effect of zona pellucida removal on DNA methylation in early mouse embryos
    Ricardo C Ribas
    Roslin Institute, Roslin, Midlothian EH25 9PS, United Kingdom
    Biol Reprod 74:307-13. 2006
    ..Similarly, no differences were found regarding the onset of transcription of Dnmt1s, Nanog, and Fgf4 genes...
  22. ncbi Somatic cell nuclear transfer: recent progress and challenges
    Andras Dinnyes
    Department of Gene Expression and Development, Roslin Institute, Midlothian, United Kingdom
    Cloning Stem Cells 4:81-90. 2002
    ..Key problems hindering further progress are addressed...
  23. ncbi Cloned lambs--lessons from pathology
    Susan M Rhind
    University of Edinburgh, Veterinary Pathology, Easter Bush Veterinary Centre, Roslin, Midlothian EH25 9RG, UK
    Nat Biotechnol 21:744-5. 2003
  24. ncbi Immunohistochemical characterization of cloned lamb nephropathy
    Andrew J Dawson
    Dept of Veterinary Pathology, Easter Bush Veterinary Centre, Roslin, Midlothian, Scotland, UK EH25 9RG
    J Histochem Cytochem 52:1657-64. 2004
    ..In addition, the study has confirmed that the changes in cloned lamb nephropathy are established by day 85 of development...
  25. ncbi Effect of cell confluence on production of cloned mice using an inbred embryonic stem cell line
    Shaorong Gao
    Department of Gene Expression and Development, The Roslin Institute, Roslin, Midlothian EH25 9PS, Scotland, United Kingdom
    Biol Reprod 68:595-603. 2003
    ..These results also demonstrate that the inbred ES cell line, HM-1, can be used to produce viable cloned mice, although less efficiently than most heterozygous ES cell lines...
  26. ncbi Improvement of an electrical activation protocol for porcine oocytes
    Jie Zhu
    Department of Gene Expression and Development, Roslin Institute, Roslin, Midlothian EH25 9PS, United Kingdom
    Biol Reprod 66:635-41. 2002
    ..0 kV/cm DC. Approximately 84% of parthenogenetic blastocysts yielded by this protocol were diploid, implying a potential for further in vivo development...
  27. ncbi The moral imperative for human cloning
    Ian Wilmut
    Roslin Institute, Edinburgh, UK
    New Sci 181:16-7. 2004
  28. ncbi Application of reproductive biotechnology in animals: implications and potentials. Applications of reproductive cloning
    Lesley Paterson
    Roslin Institute, Roslin, Midlothian, Scotland EH25 9PS, UK
    Anim Reprod Sci 79:137-43. 2003
    ..This has potential application in the production of organs for transplantation to humans, studies of human genetic disease and basic research in to the control of gene expression and function...
  29. ncbi Human cloning: can it be made safe?
    Susan M Rhind
    Department of Veterinary Pathology, Royal Dick School of Veterinary Studies, University of Edinburgh, Easter Bush Veterinary Centre, Roslin, Midlothian EH25 9RG, UK
    Nat Rev Genet 4:855-64. 2003
    ..Human reproductive cloning is unethical, but the production of cells from cloned embryos could offer many potential benefits. So, can human cloning be made safe?..
  30. ncbi A new era in methods of embryo manipulation?
    Ian Wilmut
    Cloning Stem Cells 5:1-2. 2003
  31. ncbi What regulates developmental plasticity?
    Ian Wilmut
    Cloning Stem Cells 4:177-8. 2002
  32. ncbi Effects of donor oocytes and culture conditions on development of cloned mice embryos
    Shaorong Gao
    Department of Gene Expression and Development, The Roslin Institute, Roslin, Scotland, United Kingdom
    Mol Reprod Dev 66:126-33. 2003
    ..M16 medium is superior for culture of nuclear transfer embryos and low oxygen concentration with CZB medium during culture shows no benefit on development of cloned embryos...
  33. pmc The effect of interspecific oocytes on demethylation of sperm DNA
    Nathalie Beaujean
    Division of Gene Expression and Development, Roslin Institute, Roslin EH25 9PS, United Kingdom
    Proc Natl Acad Sci U S A 101:7636-40. 2004
    ..Our results suggest that the murine demethylation process is facilitated either by a sperm-derived factor or by male pronuclear chromatin composition...
  34. ncbi In vitro and in vivo developmental competence of ovulated and in vitro matured porcine oocytes activated by electrical activation
    Jie Zhu
    Department of Gene Expression and Development, The Roslin Institute, Midlothian, EH 25 9PS, Scotland, United Kingdom
    Cloning Stem Cells 5:355-65. 2003
    ..Parthenote fetuses collected 21 and 30 days post estrus were morphologically normal but significantly smaller and lighter than fertilized controls (p < 0.01). IVM pig parthenotes stopped development around 31 days post estrus...
  35. ncbi Cloning of mice by nuclear transfer
    Shaorong Gao
    Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140
    Cloning Stem Cells 5:287-94. 2003
  36. ncbi Cell plasticity: flexible arrangement
    Neil D Theise
    Division of Digestive Diseases, Beth Israel Medical Center, New York 10003, USA
    Nature 425:21. 2003
  37. ncbi Recent stem cell research poses several challenges
    Ian Wilmut
    Cloning Stem Cells 8:67-8. 2006
  38. ncbi Cloning and stem cells
    Ian Wilmut
    Cloning Stem Cells 4:103-4. 2002
  39. ncbi An astonishing experiment
    Ian Wilmut
    Cloning Stem Cells 8:235-6. 2006
  40. ncbi Are there any normal cloned mammals?
    Ian Wilmut
    Nat Med 8:215-6. 2002
  41. ncbi Ethics. The ISSCR guidelines for human embryonic stem cell research
    George Q Daley
    Children s Hospital, Boston, Massachusetts, USA
    Science 315:603-4. 2007
  42. ncbi Time 100 scientists & thinkers. Shinya Yamanaka & James Thomson
    Ian Wilmut
    Time 171:96. 2008
  43. pmc Rapid induction of pluripotency genes after exposure of human somatic cells to mouse ES cell extracts
    Thierry Bru
    College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    Exp Cell Res 314:2634-42. 2008
    ..This provides a methodology for studying the de-differentiation of somatic cells that can potentially lead to an efficient way of reprogramming somatic cells to a pluripotent state without genetically altering them...
  44. ncbi Stem cells: primates join the club
    Ian Wilmut
    Nature 450:485-6. 2007
  45. ncbi [Ian Wilmut abandons his cloning research]
    Jean Yves Nau
    Rev Med Suisse 3:2868. 2007
  46. doi The first direct reprogramming of adult human fibroblasts
    Ian Wilmut
    Scottish Centre for Regenerative Medicine, University of Edinburgh, Edinburgh EH16 4SB, UK
    Cell Stem Cell 1:593-4. 2007
    ..Refinements of this procedure will make it possible to produce pluripotent human cell lines without use of an embryo. There are profound scientific and social implications of this research...
  47. ncbi Stem cell research. Cloning pioneer heads toward human frontier
    Gretchen Vogel
    Science 298:37-9. 2002
  48. ncbi Islet-like cells derived from murine ES cells: an important clarification
    Ian Wilmut
    Cloning Stem Cells 7:207-8. 2005
  49. ncbi Human embryonic stem cells
    Ian Wilmut
    Science 310:1903. 2005
  50. pmc Nuclear transfer in rodents
    Linda J Mullins
    Molecular Physiology Laboratory, University of Edinburgh Medical School, Edinburgh EH8 9AG, UK
    J Physiol 554:4-12. 2004
    ..Finally, we will summarize the emerging principles which appear to govern nuclear reprogramming and production of clones, and will consider the application of nuclear transfer to the rat...
  51. ncbi Special methods issues
    Ian Wilmut
    Cloning Stem Cells 5:221. 2003
  52. pmc Human cells from cloned embryos in research and therapy
    Ian Wilmut
    BMJ 328:415-6. 2004
  53. ncbi Effect of limited DNA methylation reprogramming in the normal sheep embryo on somatic cell nuclear transfer
    Nathalie Beaujean
    Division of Gene Expression and Development, Roslin Institute, Roslin EH25 9PS, United Kingdom
    Biol Reprod 71:185-93. 2004
    ..Thus, both remodeling of DNA and epigenetic reprogramming appear critical for development of both fertilized and nuclear transfer embryos...
  54. ncbi Conservation of IGF2-H19 and IGF2R imprinting in sheep: effects of somatic cell nuclear transfer
    Lorraine E Young
    School of Human Development, University of Nottingham, Queens Medical Centre, Nottingham, UK
    Mech Dev 120:1433-42. 2003
    ..Although the underlying mechanisms remain to be determined, our data indicate that somatic cell nuclear transfer procedures can lead to epigenetic deregulation at imprinted loci...
  55. ncbi The likely effect of being a clone
    Ian Wilmut
    Cloning Stem Cells 6:209-10. 2004
  56. ncbi Dolly-her life and legacy
    Ian Wilmut
    Cloning Stem Cells 5:99-100. 2003
  57. ncbi The search for cells that heal. The creator of Dolly the cloned sheep asks that society look past the controversies to the ultimate payoff
    Ian Wilmut
    University of Edinburgh, Scotland
    Sci Am 293:A35. 2005
  58. ncbi Cloned dogs: another Korean success!
    Ian Wilmut
    Cloning Stem Cells 7:139-40. 2005
  59. ncbi Human cells from cloned embryos in research and therapy
    Ian Wilmut
    Cloning Stem Cells 5:163-4. 2003
  60. ncbi Variations in humanized and defined culture conditions supporting derivation of new human embryonic stem cell lines
    Judy M Fletcher
    Division of Gene Function and Development, Roslin Institute, Scotland, United Kingdom
    Cloning Stem Cells 8:319-34. 2006
    ..This represents another sequential step in the generation of therapeutic grade stem cells with reduced risk of zoonotic pathogen transmission...