Michael J Taussig

Summary

Affiliation: Institute for Animal Health
Country: UK

Publications

  1. ncbi request reprint Eukaryotic ribosome display with in situ DNA recovery
    Mingyue He
    Technology Research Group, The Babraham Institute, Cambridge CB22 4AT, UK
    Nat Methods 4:281-8. 2007
  2. ncbi request reprint ProteomeBinders: planning a European resource of affinity reagents for analysis of the human proteome
    Michael J Taussig
    Technology Research Group, The Babraham Institute, Cambridge CB22 3AT, UK
    Nat Methods 4:13-7. 2007
  3. doi request reprint Eukaryotic ribosome display with in situ DNA recovery
    Mingyue He
    The Inositide Laboratory, The Babraham Institute, Cambridge, UK
    Methods Mol Biol 805:75-85. 2012
  4. doi request reprint Cell free expression put on the spot: advances in repeatable protein arraying from DNA (DAPA)
    Oda Stoevesandt
    Protein Technology Group, Babraham Bioscience Technologies, Cambridge CB22 3AT, UK
    N Biotechnol 28:282-90. 2011
  5. pmc Effects of mutation at the D-JH junction on affinity, specificity, and idiotypy of anti-progesterone antibody DB3
    Mingyue He
    Technology Research Group, The Babraham Institute, Cambridge, United Kingdom
    Protein Sci 15:2141-8. 2006
  6. doi request reprint Protein microarrays: high-throughput tools for proteomics
    Oda Stoevesandt
    Babraham Bioscience Technologies Ltd, Babraham Research Campus, Cambridge, CB22 3AT, UK
    Expert Rev Proteomics 6:145-57. 2009
  7. doi request reprint Production of protein arrays by cell-free systems
    Mingyue He
    Technology Research Group, The Babraham Institute, Cambridge, UK
    Methods Mol Biol 484:207-15. 2008
  8. doi request reprint In situ synthesis of protein arrays
    Mingyue He
    Technology Research Group, The Babraham Institute, Cambridge CB22 3AT, UK
    Curr Opin Biotechnol 19:4-9. 2008
  9. doi request reprint Detection of protein-protein interactions by ribosome display and protein in situ immobilisation
    Mingyue He
    The Babraham Institute, Babraham Research Campus, Cambridge CB22 3AT, UK
    N Biotechnol 26:277-81. 2009
  10. ncbi request reprint Affinity reagent resources for human proteome detection: initiatives and perspectives
    Oda Stoevesandt
    Technology Research Group, The Babraham Institute, Cambridge, UK
    Proteomics 7:2738-50. 2007

Collaborators

Detail Information

Publications26

  1. ncbi request reprint Eukaryotic ribosome display with in situ DNA recovery
    Mingyue He
    Technology Research Group, The Babraham Institute, Cambridge CB22 4AT, UK
    Nat Methods 4:281-8. 2007
    ..We also introduce a recent, previously unpublished improvement to the procedure in which in situ reverse transcription is combined with sensitive single-primer PCR technology...
  2. ncbi request reprint ProteomeBinders: planning a European resource of affinity reagents for analysis of the human proteome
    Michael J Taussig
    Technology Research Group, The Babraham Institute, Cambridge CB22 3AT, UK
    Nat Methods 4:13-7. 2007
    ..Given the huge diversity of the proteome, the scale of the project is potentially immense but nevertheless feasible in the context of a pan-European or even worldwide coordination...
  3. doi request reprint Eukaryotic ribosome display with in situ DNA recovery
    Mingyue He
    The Inositide Laboratory, The Babraham Institute, Cambridge, UK
    Methods Mol Biol 805:75-85. 2012
    ....
  4. doi request reprint Cell free expression put on the spot: advances in repeatable protein arraying from DNA (DAPA)
    Oda Stoevesandt
    Protein Technology Group, Babraham Bioscience Technologies, Cambridge CB22 3AT, UK
    N Biotechnol 28:282-90. 2011
    ..The capabilities of DAPA technology in comparison with other protein array methods are discussed...
  5. pmc Effects of mutation at the D-JH junction on affinity, specificity, and idiotypy of anti-progesterone antibody DB3
    Mingyue He
    Technology Research Group, The Babraham Institute, Cambridge, United Kingdom
    Protein Sci 15:2141-8. 2006
    ....
  6. doi request reprint Protein microarrays: high-throughput tools for proteomics
    Oda Stoevesandt
    Babraham Bioscience Technologies Ltd, Babraham Research Campus, Cambridge, CB22 3AT, UK
    Expert Rev Proteomics 6:145-57. 2009
    ..This article reviews recent developments in the generation of protein microarrays and their applications in proteomics and diagnostics...
  7. doi request reprint Production of protein arrays by cell-free systems
    Mingyue He
    Technology Research Group, The Babraham Institute, Cambridge, UK
    Methods Mol Biol 484:207-15. 2008
    ..Advantages of PISA include avoiding bacterial expression and protein purification and making functional protein arrays available as required from genetic information...
  8. doi request reprint In situ synthesis of protein arrays
    Mingyue He
    Technology Research Group, The Babraham Institute, Cambridge CB22 3AT, UK
    Curr Opin Biotechnol 19:4-9. 2008
    ..Recently employed methods reviewed are PISA (protein in situ array) and NAPPA (nucleic acid programmable protein array) from DNA and puromycin-mediated immobilisation from mRNA...
  9. doi request reprint Detection of protein-protein interactions by ribosome display and protein in situ immobilisation
    Mingyue He
    The Babraham Institute, Babraham Research Campus, Cambridge CB22 3AT, UK
    N Biotechnol 26:277-81. 2009
    ..The method has promise for library screening of pairwise protein interactions, down to the analytical level of individual domain or motif mapping...
  10. ncbi request reprint Affinity reagent resources for human proteome detection: initiatives and perspectives
    Oda Stoevesandt
    Technology Research Group, The Babraham Institute, Cambridge, UK
    Proteomics 7:2738-50. 2007
    ..The benefits of such reagent resources will be seen in basic research, medicine and the biotechnology and pharmaceutical industries...
  11. ncbi request reprint Ribosome display of antibodies: expression, specificity and recovery in a eukaryotic system
    Mingyue He
    Protein Technologies Laboratory, The Babraham Institute, The Babraham Research Campus, Cambridge CB2 4AT, UK
    J Immunol Methods 297:73-82. 2005
    ..Our findings confirm the effectiveness of the eukaryotic ribosome display system and define conditions for efficient selection of single chain antibodies...
  12. doi request reprint Optimised 'on demand' protein arraying from DNA by cell free expression with the 'DNA to Protein Array' (DAPA) technology
    Ronny Schmidt
    Protein Technology Group, Babraham Bioscience Technologies Ltd, Babraham Research Campus, Cambridge CB22 3AT, UK
    J Proteomics 88:141-8. 2013
    ..The amount of expressed protein in DAPA was comparable to those of classically spotted protein arrays. Reaction conditions can be tailored to suit the application of interest...
  13. doi request reprint Selection of recombinant antibodies by eukaryotic ribosome display
    Mingyue He
    Technology Research Group, The Babraham Institute, Cambridge, UK
    Methods Mol Biol 484:193-205. 2008
    ..We describe our eukaryotic system, in which rabbit reticulocyte extracts are used for cell free transcription/translation and cDNA is recovered by in situ RT-PCR performed on the selected complexes...
  14. pmc Enhanced cell-free protein expression by fusion with immunoglobulin Ckappa domain
    Elizabeth Palmer
    Technology Research Group, The Babraham Institute, Cambridge CB2 4AT, United Kingdom
    Protein Sci 15:2842-6. 2006
    ....
  15. doi request reprint Printing protein arrays from DNA arrays
    Mingyue He
    Technology Research Group, The Babraham Institute, Cambridge CB22 3AT, UK
    Nat Methods 5:175-7. 2008
    ..DAPA eliminates the need for separate protein expression, purification and spotting, and also overcomes the problem of long-term functional storage of surface-bound proteins...
  16. pmc Heavy chain-only antibodies are spontaneously produced in light chain-deficient mice
    Xiangang Zou
    The Babraham Institute, Babraham, Cambridge CB22 3AT, England, UK
    J Exp Med 204:3271-83. 2007
    ..Thus, naturally occurring H chain transcripts without C(H)1 (V(H)DJ(H)-hinge-C(H)2-C(H)3) are selected for and lead to the formation of fully functional and diverse H chain-only antibodies in L(-/-) animals...
  17. ncbi request reprint Double-hexahistidine tag with high-affinity binding for protein immobilization, purification, and detection on ni-nitrilotriacetic acid surfaces
    Farid Khan
    Protein Technologies Laboratory, The Babraham Institute, Babraham Research Campus, Cambridge CB2 4AT, UK
    Anal Chem 78:3072-9. 2006
    ..The double-His6 peptide has the potential to be a universal tag for protein immobilization and detection on arrays and single-step purification of proteins from crude mixtures...
  18. ncbi request reprint DiscernArray technology: a cell-free method for the generation of protein arrays from PCR DNA
    Mingyue He
    Discerna Limited, Babraham Research Campus, Babraham, CB2 4AT, Cambridge, UK
    J Immunol Methods 274:265-70. 2003
    ..DiscernArray is particularly useful for arraying proteins and domains which cannot be functionally produced in heterologous expression systems or for which the cloned DNA is not available...
  19. doi request reprint New Biotechnology and the European Federation of Biotechnology
    Michael J Taussig
    N Biotechnol 25:1-2. 2008
  20. ncbi request reprint Selection of antigenic markers on a GFP-Ckappa fusion scaffold with high sensitivity by eukaryotic ribosome display
    Yong Min Yang
    The Institute of Genetics, San Diego, CA 92121 2233, USA
    Biochem Biophys Res Commun 359:251-7. 2007
    ..This platform is highly specific and sensitive for the antigen-antibody interaction and may permit selection and reshaping of high affinity antigenic variants of scaffold proteins...
  21. ncbi request reprint Crystal structure of a human autoimmune complex between IgM rheumatoid factor RF61 and IgG1 Fc reveals a novel epitope and evidence for affinity maturation
    Stephane Duquerroy
    Virologie Moléculaire et Structurale, CNRS UMR 2472 INRA UMR 1157, 91198 Gif sur Yvette, France
    J Mol Biol 368:1321-31. 2007
    ..The antibody contacts also involve two somatically mutated V(H) residues, reinforcing the suggestion of a process of antigen-driven maturation and selection for IgG Fc during the generation of this RF autoantibody...
  22. ncbi request reprint Improved affinity coupling for antibody microarrays: engineering of double-(His)6-tagged single framework recombinant antibody fragments
    Cornelia Steinhauer
    Department of Immunotechnology, Lund University, Lund, Sweden
    Proteomics 6:4227-34. 2006
    ..Taken together, the results demonstrate the generic potential of double-(His)(6)-tag recombinant antibodies for the facile fabrication of high-density antibody microarrays...
  23. ncbi request reprint Ribosome display: cell-free protein display technology
    Mingyue He
    Discerna Limited, Babraham, Cambridge, UK
    Brief Funct Genomic Proteomic 1:204-12. 2002
    ..Both prokaryotic and eukaryotic ribosome display systems have been developed and each has its own distinctive features. In this paper, ribosome display systems and their application in selection and evolution of proteins are reviewed...
  24. ncbi request reprint Production of human single-chain antibodies by ribosome display
    Mingyue He
    Discerna Ltd, Babraham Hall, Babraham, Cambridge, UK
    Methods Mol Biol 248:177-89. 2004
  25. ncbi request reprint Scaffolds for protein crystallisation
    Enrico A Stura
    CEA, Département d Ingénierie des Protéines DIEP, CE Saclay, 91191 Gif sur Yvette, France
    Acta Crystallogr D Biol Crystallogr 58:1715-21. 2002
    ..Hence it may be possible to generalize the method to include bacterial expression of fusion proteins with either protein A or protein G as the fusion partner...
  26. ncbi request reprint Evidence for plasticity and structural mimicry at the immunoglobulin light chain-protein L interface
    Marc Graille
    Laboratoire de Structure des Protéines, Département d Ingénierie et d Etudes des Protéines DIEP, Commissariat a l Energie Atomique, Centre d Etudes de Saclay, Gif sur Yvette, France
    J Biol Chem 277:47500-6. 2002
    ..These sites exhibit a remarkable structural mimicry of growth factors binding to their receptors. This could explain the protein L superantigenic activity on human B lymphocytes...