Research Topics
| Michael J TaussigSummaryAffiliation: Institute for Animal Health Country: UK Publications
| Collaborators
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Detail Information
Publications
Eukaryotic ribosome display with in situ DNA recoveryMingyue He
Technology Research Group, The Babraham Institute, Cambridge CB22 4AT, UK
Nat Methods 4:281-8. 2007..We also introduce a recent, previously unpublished improvement to the procedure in which in situ reverse transcription is combined with sensitive single-primer PCR technology...- ProteomeBinders: planning a European resource of affinity reagents for analysis of the human proteomeMichael J Taussig
Technology Research Group, The Babraham Institute, Cambridge CB22 3AT, UK
Nat Methods 4:13-7. 2007..Given the huge diversity of the proteome, the scale of the project is potentially immense but nevertheless feasible in the context of a pan-European or even worldwide coordination... 
Eukaryotic ribosome display with in situ DNA recoveryMingyue He
The Inositide Laboratory, The Babraham Institute, Cambridge, UK
Methods Mol Biol 805:75-85. 2012....- Cell free expression put on the spot: advances in repeatable protein arraying from DNA (DAPA)Oda Stoevesandt
Protein Technology Group, Babraham Bioscience Technologies, Cambridge CB22 3AT, UK
N Biotechnol 28:282-90. 2011..The capabilities of DAPA technology in comparison with other protein array methods are discussed... - Protein microarrays: high-throughput tools for proteomicsOda Stoevesandt
Babraham Bioscience Technologies Ltd, Babraham Research Campus, Cambridge, CB22 3AT, UK
Expert Rev Proteomics 6:145-57. 2009..This article reviews recent developments in the generation of protein microarrays and their applications in proteomics and diagnostics... 
Effects of mutation at the D-JH junction on affinity, specificity, and idiotypy of anti-progesterone antibody DB3Mingyue He
Technology Research Group, The Babraham Institute, Cambridge, United Kingdom
Protein Sci 15:2141-8. 2006....- Affinity reagent resources for human proteome detection: initiatives and perspectivesOda Stoevesandt
Technology Research Group, The Babraham Institute, Cambridge, UK
Proteomics 7:2738-50. 2007..The benefits of such reagent resources will be seen in basic research, medicine and the biotechnology and pharmaceutical industries... - In situ synthesis of protein arraysMingyue He
Technology Research Group, The Babraham Institute, Cambridge CB22 3AT, UK
Curr Opin Biotechnol 19:4-9. 2008..Recently employed methods reviewed are PISA (protein in situ array) and NAPPA (nucleic acid programmable protein array) from DNA and puromycin-mediated immobilisation from mRNA... - Production of protein arrays by cell-free systemsMingyue He
Technology Research Group, The Babraham Institute, Cambridge, UK
Methods Mol Biol 484:207-15. 2008..Advantages of PISA include avoiding bacterial expression and protein purification and making functional protein arrays available as required from genetic information... - Detection of protein-protein interactions by ribosome display and protein in situ immobilisationMingyue He
The Babraham Institute, Babraham Research Campus, Cambridge CB22 3AT, UK
N Biotechnol 26:277-81. 2009..The method has promise for library screening of pairwise protein interactions, down to the analytical level of individual domain or motif mapping... - Ribosome display of antibodies: expression, specificity and recovery in a eukaryotic systemMingyue He
Protein Technologies Laboratory, The Babraham Institute, The Babraham Research Campus, Cambridge CB2 4AT, UK
J Immunol Methods 297:73-82. 2005..Our findings confirm the effectiveness of the eukaryotic ribosome display system and define conditions for efficient selection of single chain antibodies... - Selection of recombinant antibodies by eukaryotic ribosome displayMingyue He
Technology Research Group, The Babraham Institute, Cambridge, UK
Methods Mol Biol 484:193-205. 2008..We describe our eukaryotic system, in which rabbit reticulocyte extracts are used for cell free transcription/translation and cDNA is recovered by in situ RT-PCR performed on the selected complexes... - Enhanced cell-free protein expression by fusion with immunoglobulin Ckappa domainElizabeth Palmer
Technology Research Group, The Babraham Institute, Cambridge CB2 4AT, United Kingdom
Protein Sci 15:2842-6. 2006.... - Printing protein arrays from DNA arraysMingyue He
Technology Research Group, The Babraham Institute, Cambridge CB22 3AT, UK
Nat Methods 5:175-7. 2008..DAPA eliminates the need for separate protein expression, purification and spotting, and also overcomes the problem of long-term functional storage of surface-bound proteins... - Heavy chain-only antibodies are spontaneously produced in light chain-deficient miceXiangang Zou
The Babraham Institute, Babraham, Cambridge CB22 3AT, England, UK
J Exp Med 204:3271-83. 2007..Thus, naturally occurring H chain transcripts without C(H)1 (V(H)DJ(H)-hinge-C(H)2-C(H)3) are selected for and lead to the formation of fully functional and diverse H chain-only antibodies in L(-/-) animals... - Double-hexahistidine tag with high-affinity binding for protein immobilization, purification, and detection on ni-nitrilotriacetic acid surfacesFarid Khan
Protein Technologies Laboratory, The Babraham Institute, Babraham Research Campus, Cambridge CB2 4AT, UK
Anal Chem 78:3072-9. 2006..The double-His6 peptide has the potential to be a universal tag for protein immobilization and detection on arrays and single-step purification of proteins from crude mixtures... - DiscernArray technology: a cell-free method for the generation of protein arrays from PCR DNAMingyue He
Discerna Limited, Babraham Research Campus, Babraham, CB2 4AT, Cambridge, UK
J Immunol Methods 274:265-70. 2003..DiscernArray is particularly useful for arraying proteins and domains which cannot be functionally produced in heterologous expression systems or for which the cloned DNA is not available... - New Biotechnology and the European Federation of BiotechnologyMichael J Taussig
N Biotechnol 25:1-2. 2008 - Selection of antigenic markers on a GFP-Ckappa fusion scaffold with high sensitivity by eukaryotic ribosome displayYong Min Yang
The Institute of Genetics, San Diego, CA 92121 2233, USA
Biochem Biophys Res Commun 359:251-7. 2007..This platform is highly specific and sensitive for the antigen-antibody interaction and may permit selection and reshaping of high affinity antigenic variants of scaffold proteins... - Crystal structure of a human autoimmune complex between IgM rheumatoid factor RF61 and IgG1 Fc reveals a novel epitope and evidence for affinity maturationStephane Duquerroy
Virologie Moléculaire et Structurale, CNRS UMR 2472 INRA UMR 1157, 91198 Gif sur Yvette, France
J Mol Biol 368:1321-31. 2007..The antibody contacts also involve two somatically mutated V(H) residues, reinforcing the suggestion of a process of antigen-driven maturation and selection for IgG Fc during the generation of this RF autoantibody... - Improved affinity coupling for antibody microarrays: engineering of double-(His)6-tagged single framework recombinant antibody fragmentsCornelia Steinhauer
Department of Immunotechnology, Lund University, Lund, Sweden
Proteomics 6:4227-34. 2006..Taken together, the results demonstrate the generic potential of double-(His)(6)-tag recombinant antibodies for the facile fabrication of high-density antibody microarrays... - Ribosome display: cell-free protein display technologyMingyue He
Discerna Limited, Babraham, Cambridge, UK
Brief Funct Genomic Proteomic 1:204-12. 2002..Both prokaryotic and eukaryotic ribosome display systems have been developed and each has its own distinctive features. In this paper, ribosome display systems and their application in selection and evolution of proteins are reviewed... - Production of human single-chain antibodies by ribosome displayMingyue He
Discerna Ltd, Babraham Hall, Babraham, Cambridge, UK
Methods Mol Biol 248:177-89. 2004 - Scaffolds for protein crystallisationEnrico A Stura
CEA, Département d Ingénierie des Protéines DIEP, CE Saclay, 91191 Gif sur Yvette, France
Acta Crystallogr D Biol Crystallogr 58:1715-21. 2002..Hence it may be possible to generalize the method to include bacterial expression of fusion proteins with either protein A or protein G as the fusion partner... - Evidence for plasticity and structural mimicry at the immunoglobulin light chain-protein L interfaceMarc Graille
, , , Centre d'Etudes de Saclay, Gif-sur-Yvette, France
J Biol Chem 277:47500-6. 2002..These sites exhibit a remarkable structural mimicry of growth factors binding to their receptors. This could explain the protein L superantigenic activity on human B lymphocytes...