Research Topics
| R Gary SawersSummaryAffiliation: Institute for Animal Health Country: UK Publications
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Detail Information
Publications
A glycyl radical solution: oxygen-dependent interconversion of pyruvate formate-lyaseG Sawers
Nitrogen Fixation Laboratory, John Innes Centre, Norwich, UK
Mol Microbiol 29:945-54. 1998..It is becoming increasingly apparent that glycyl radical enzymes are more prevalent than previously surmised. They represent a class of enzymes with unusual biochemistry and probably predate the appearance of molecular oxygen...- Formate and its role in hydrogen production in Escherichia coliR G Sawers
Department of Molecular Microbiology, John Innes Centre, Norwich NR4 7UH, UK
Biochem Soc Trans 33:42-6. 2005..Formate, therefore, represents a signature molecule in the fermenting E. coli cell and factors that determine formate metabolism control FHL synthesis and consequently dihydrogen evolution... - Transcript analysis of Escherichia coli K-12 insertion element IS5R Gary Sawers
Department of Molecular Microbiology, John Innes Centre, Norwich NR4 7UH, United Kingdom
FEMS Microbiol Lett 244:397-401. 2005..This suggests that IS5-specific transcription can be influenced by the specific location of the element in the chromosome, the orientation it adopts and the gene it interrupts... - Expression of fnr is constrained by an upstream IS5 insertion in certain Escherichia coli K-12 strainsR Gary Sawers
Department of Molecular Microbiology, John Innes Centre, Norwich NR4 7UH, UK
J Bacteriol 187:2609-17. 2005..Expression of some operons, e.g., focA-pfl and fdnGHJI, was unaffected by reduction in the level of FNR, while the expression of other genes such as ndh and nikA was clearly affected... - A novel mechanism controls anaerobic and catabolite regulation of the Escherichia coli tdc operonG Sawers
Department of Molecular Microbiology, John Innes Centre, Norwich NR4 7UH, UK
Mol Microbiol 39:1285-98. 2001..Remarkably, however, anaerobic regulation of the mutant promoter was retained. This indicates that the other tdc regulators, TdcA and TdcR, govern anaerobic transcription activation by CRP... - The aerobic/anaerobic interfaceG Sawers
Nitrogen Fixation Laboratory, John Innes Centre, Norwich, NR4 7UH, UK
Curr Opin Microbiol 2:181-7. 1999..The factors involved in these diverse sensory systems are proving to have a pervasive impact on controlling gene expression and cellular physiology. Similar signal transduction systems are prevalent in a diverse range of microorganisms... - The anaerobic degradation of L-serine and L-threonine in enterobacteria: networks of pathways and regulatory signalsG Sawers
Nitrogen Fixation Laboratory, John Innes Centre, Norwich NR4 7UH, UK
Arch Microbiol 171:1-5. 1998..Studies on this pathway are beginning to provide insights into how an organism adapts its genetic makeup to meet the physiological demands of the cell... - The glycyl radical enzyme TdcE can replace pyruvate formate-lyase in glucose fermentationG Sawers
Nitrogen Fixation Laboratory, John Innes Centre, Norwich, United Kingdom
J Bacteriol 180:3509-16. 1998..Hesslinger, S. A. Fairhurst, and G. Sawers, Mol. Microbiol. 27:477-492, 1998) that can accept pyruvate as an enzyme substrate. Here we show that TdcE is directly responsible for the restoration of fermentative growth to pfl mutants... - Aerobic activation of transcription of the anaerobically inducible Escherichia coli focA-pfl operon by fumarate nitrate regulatorFrancisca Reyes-Ramirez
Department of Molecular Microbiology, John Innes Centre, Norwich, UK
FEMS Microbiol Lett 255:262-7. 2006.... - Coordinate synthesis of azurin I and copper nitrite reductase in Alcaligenes xylosoxidans during denitrificationRoger L Harris
Department of Molecular Microbiology, John Innes Centre, Norwich, UK
Arch Microbiol 186:241-9. 2006..Taken together, these findings indicate that in A. xylosoxidans azuA expression is coordinated with denitrification and recombinant Az I is processed and matured in the periplasm of E. coli in the same way it is in A. xylosoxidans... - Evidence for novel processing of the anaerobically inducible dicistronic focA-pfl mRNA transcript in Escherichia coliR Gary Sawers
Department of Molecular Microbiology, John Innes Centre, Norwich NR4 7UH, UK
Mol Microbiol 58:1441-53. 2005..Moreover, processing was independent of focA translation. Taken together, these data suggest that processing of the focA-pfl transcripts occurs by a novel mechanism that might require the action of E. coli core RNA polymerase... - Differential turnover of the multiple processed transcripts of the Escherichia coli focA-pflB operonR Gary Sawers
Department of Molecular Microbiology, John Innes Centre, Norwich, UK
Microbiology 152:2197-205. 2006.... - Extreme arsenic resistance by the acidophilic archaeon 'Ferroplasma acidarmanus' Fer1Craig Baker Austin
School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, UK
Extremophiles 11:425-34. 2007..This report represents the first molecular and proteomic study of arsenic resistance in an acidophilic archaeon... - Characterization of cycP gene expression in Achromobacter xylosoxidans NCIMB 11015 and high-level heterologous synthesis of cytochrome c' in Escherichia coliRoger L Harris
John Innes Centre, Norwich, UK
J Mol Microbiol Biotechnol 18:102-8. 2010..xylosoxidans. This system provides an excellent platform to facilitate biochemical and structural dissection of the mechanism underlying the novel specificity of NO binding to the proximal face of the haem... - Transcriptional activation by FNR and CRP: reciprocity of binding-site recognitionG Sawers
Nitrogen Fixation Laboratory, John Innes Centre, Norwich, UK
Mol Microbiol 23:835-45. 1997..FNR also activated transcription from the CF-site in vitro, giving further support to the idea that a single functional DNA half-site is sufficient to direct binding and transcription activation by a dimeric transcription factor... - dpp genes of Rhizobium leguminosarum specify uptake of delta-aminolevulinic acidR A Carter
School of Biological Sciences, University of East Anglia, Norwich, UK
Mol Plant Microbe Interact 15:69-74. 2002..The dppABCDF promoter was mapped and is most likely recognized by sigma70... - The obligate aerobic actinomycete Streptomyces coelicolor A3(2) survives extended periods of anaerobic stressGeertje van Keulen
Department of Molecular Microbiology, John Innes Centre, Norwich NR4 7UH, UK
Environ Microbiol 9:3143-9. 2007.... - The crystal structure of Escherichia coli TdcF, a member of the highly conserved YjgF/YER057c/UK114 familyJulia D Burman
Department of Biological Chemistry, John Innes Centre, Norwich, UK
BMC Struct Biol 7:30. 2007..The gene encoding the TdcF protein of E. coli, a YjgF/YER057c/UK114 family member, resides in an operon that strongly suggests a role in the metabolism of 2-ketobutyrate for this protein... - Heterologous metalloprotein biosynthesis in Escherichia coli: conditions for the overproduction of functional copper-containing nitrite reductase and azurin from Alcaligenes xylosoxidansRoger L Harris
Department of Molecular Microbiology, John Innes Centre, Norwich NR4 7UH, UK
J Synchrotron Radiat 12:13-8. 2005..xylosoxidans. The findings of this work have important implications for the overproduction of recombinant metalloproteins in heterologous hosts... - The iron-sulfur cluster in the L-serine dehydratase TdcG from Escherichia coli is required for enzyme activityJulia D Burman
Department of Molecular Microbiology, John Innes Centre, Norwich NR4 7UH, UK
FEBS Lett 576:442-4. 2004..A concomitant loss of dehydratase activity was demonstrated, indicating that integrity of the [4Fe-4S]2+ cluster is essential for enzyme activity... - Quorum-sensing-regulated transcriptional initiation of plasmid transfer and replication genes in Rhizobium leguminosarum biovar viciaeCraig McAnulla
Department of Molecular Microbiology, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK
Microbiology 153:2074-82. 2007.... - Gas vesicles in actinomycetes: old buoys in novel habitats?Geertje van Keulen
Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, Norwich, UK
Trends Microbiol 13:350-4. 2005..Here, we discuss whether putative gas vesicle proteins in these actinomycetes might actually be involved in flotation or whether they might fulfil other cellular functions... - Molecular insight into extreme copper resistance in the extremophilic archaeon 'Ferroplasma acidarmanus' Fer1Craig Baker-Austin
School of Biological Sciences, University of East Anglia, Norwich, UK
Microbiology 151:2637-46. 2005.... - Maturation of [NiFe]-hydrogenases in Escherichia coliLucia Forzi
Max Planck Institute for Terrestrial Microbiology, Karl von Frisch Strasse, 35043 Marburg, Germany
Biometals 20:565-78. 2007..What is becoming increasingly clear is that the metabolic origins of the carbonyl group may be different... - The CO and CN(-) ligands to the active site Fe in [NiFe]-hydrogenase of Escherichia coli have different metabolic originsLucia Forzi
Max Planck Institut fur terrestrische Mikrobiologie, Karl von Frisch Str, Marburg, Germany
FEBS Lett 581:3317-21. 2007..After growth of E. coli in the presence of (13)CO only the CO ligand was labelled with (13)C. Labelling did not result from an exchange of the intrinsic CO ligand with the exogenous CO... - Insight into catalysis of nitrous oxide reductase from high-resolution structures of resting and inhibitor-bound enzyme from Achromobacter cycloclastesKonstantinos Paraskevopoulos
School of Biomolecular Sciences, Liverpool John Moores University, Liverpool L3 5AF, UK
J Mol Biol 362:55-65. 2006..These structures, together with the published structural and spectroscopic data, give fresh insight into the mode of substrate binding, reduction and catalysis... - Modulation of NO binding to cytochrome c' by distal and proximal haem pocket residuesSonia Barbieri
Molecular Biophysics Group, STFC Daresbury Laboratory, Warrington, Cheshire WA4 4AD, UK
J Biol Inorg Chem 13:531-40. 2008..In contrast, the R124L substitution retained the property of the native protein in the initial formation of a six-coordinate NO adduct, a finding of functional importance since a Lys or an Arg residue is invariant in these proteins... - The structure of the Met144Leu mutant of copper nitrite reductase from Alcaligenes xylosoxidans provides the first glimpse of a protein-protein complex with azurin IIKonstantinos Paraskevopoulos
Molecular Biophysics Group, STFC Daresbury Laboratory, Warrington, UK
J Biol Inorg Chem 12:789-96. 2007....