Genomes and Genes
Lori A Passmore
Affiliation: Institute of Cancer Research
- Getting into position: the catalytic mechanisms of protein ubiquitylationLori A Passmore
Section of Structural Biology, The Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK
Biochem J 379:513-25. 2004..In particular, we discuss the complexity of molecular recognition required to impose selectivity on substrate selection and topology of poly-ubiquitin chains...
- The anaphase-promoting complex (APC): the sum of its parts?L A Passmore
Section of Structural Biology, Chester Beatty Laboratories, The Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK
Biochem Soc Trans 32:724-7. 2004..In the present study, the structures and functions of individual APC subunits are discussed. By dissecting the roles of its parts, we hope to gain insight into the mechanism of the intact APC...
- Structural analysis of the anaphase-promoting complex reveals multiple active sites and insights into polyubiquitylationLori A Passmore
Section of Structural Biology, The Institute of Cancer Research, Chester Beatty Laboratories, London, UK
Mol Cell 20:855-66. 2005..Our data suggest that multimerisation and/or the presence of multiple active sites facilitates the APC/C's ability to elongate polyubiquitin chains...
- Purification and assay of the budding yeast anaphase-promoting complexLori A Passmore
Section of Structural Biology, Institute for Cancer Research, London SW3 6JB, UK
Methods Enzymol 398:195-219. 2005..Activity assays toward several endogenous substrates, including Clb2 and Pds1, are described. In addition, methods for observation of APC-coactivator and APC-substrate complexes by native gel electrophoresis are described...
- Coactivator functions in a stoichiometric complex with anaphase-promoting complex/cyclosome to mediate substrate recognitionLori A Passmore
Section of Structural Biology, The Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK
EMBO Rep 6:873-8. 2005..A direct and stoichiometric function for the coactivators could explain how specific substrates are recognized by APC/C in a cell-cycle-specific manner, and how coactivator stimulates APC/C ubiquitylation activity...
- Doc1 mediates the activity of the anaphase-promoting complex by contributing to substrate recognitionLori A Passmore
Section of Structural Biology and Cancer Research UK Centre for Cell and Molecular Biology, Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK
EMBO J 22:786-96. 2003..These results imply that Doc1p/Apc10 may play a role to regulate the binding of specific substrates, similar to that of the coactivators...
- The eukaryotic translation initiation factors eIF1 and eIF1A induce an open conformation of the 40S ribosomeLori A Passmore
MRC Laboratory of Molecular Biology, Hills Road, Cambridge, UK
Mol Cell 26:41-50. 2007..Our results suggest that eIF1 and eIF1A promote an open, scanning-competent preinitiation complex that closes upon start codon recognition and eIF1 release to stabilize ternary complex binding and clamp down on mRNA...