Research Topics
| D A MacKenzieSummaryAffiliation: Institute for Animal Health Country: UK Publications
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Detail Information
Publications
Strain-specific diversity of mucus-binding proteins in the adhesion and aggregation properties of Lactobacillus reuteriDonald A MacKenzie
Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, UK
Microbiology 156:3368-78. 2010..reuteri, and further demonstrated that MUB and MUB-like proteins, although present in many L. reuteri isolates, show a high genetic heterogeneity among strains...
Isolation and use of a homologous histone H4 promoter and a ribosomal DNA region in a transformation vector for the oil-producing fungus Mortierella alpinaD A MacKenzie
Institute of Food Research, Norwich Research Park, Colney, Norwich, Norfolk, NR4 7UA, United Kingdom
Appl Environ Microbiol 66:4655-61. 2000..The generation of stable, hygromycin B-resistant transformants required the incorporation of a homologous ribosomal DNA region into the transformation vector to promote chromosomal integration...
A third fatty acid delta9-desaturase from Mortierella alpina with a different substrate specificity to ole1p and ole2pDonald A MacKenzie
Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, UK
Microbiology 148:1725-35. 2002..Conversely, high levels of desaturase in the endoplasmic reticulum membrane of these yeast transformants may increase the availability of suitable monounsaturated substrates for fatty acid elongation...- Relatedness of medically important strains of Saccharomyces cerevisiae as revealed by phylogenetics and metabolomicsDonald A MacKenzie
National Collection of Yeast Cultures, Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, UK
Yeast 25:501-12. 2008..This study confirmed the link between a subset of clinical isolates and baking or probiotic strains but also highlighted that in general the clinical strains were more diverse at both the genomic and metabolic levels... - UPR-independent dithiothreitol stress-induced genes in Aspergillus nigerD A MacKenzie
Department of Food Safety Science, Institute of Food Research, Norwich Research Park, Colney, Norwich, NR4 7UA, UK
Mol Genet Genomics 274:410-8. 2005..We conclude that DTT alone does not provide for specific induction of UPR genes and that other stress conditions must also be examined... - Metabolic footprinting as a tool for discriminating between brewing yeastsGeorgina A Pope
National Collection of Yeast Cultures, Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, UK
Yeast 24:667-79. 2007..For some strains discrimination not achieved genomically was observed metabolomically... - Species-specific variation in efficacy of yeast genomic DNA isolation techniques assessed using amplified fragment length polymorphismLinda J Fuller
National Collection of Yeast Cultures, Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, UK
Anal Biochem 381:154-6. 2008..Two alternative yeast DNA purification kits, MasterPure and Y-DER, were effective with 6 of these and 2 additional species, leaving only 9 species that remained recalcitrant to yielding sufficient amounts of DNA with the required purity... - Isolation and characterisation of the acetyl-CoA carboxylase gene from Aspergillus nidulansJ Morrice
Institute of Food Research, Norwich Research Park, Colney Lane, Norwich, Norfolk, NR4 7UA, UK
Curr Genet 34:379-85. 1998..Deletion of accA in A. nidulans was unsuccessful. The failure of A. nidulans to grow in the presence of the ACC-specific inhibitor, soraphen A, supplemented with C16-18 fatty acids suggested that ACC is an essential enzyme... - Crystal structure of a mucus-binding protein repeat reveals an unexpected functional immunoglobulin binding activityDonald A MacKenzie
Institute of Food Research, Colney Lane, Norwich NR4 7UA, United Kingdom
J Biol Chem 284:32444-53. 2009..This hitherto undetected activity is consistent with the current model that antibody responses against commensal flora are of broad specificity and low affinity... - Endoplasmic reticulum stress leads to the selective transcriptional downregulation of the glucoamylase gene in Aspergillus nigerHashem Al-Sheikh
School of Biology, University of Nottingham, University Park, Nottingham NG7 2RD, UK
Mol Microbiol 53:1731-42. 2004..niger. Although the transcription of glaA was attenuated in that strain of A. niger, UPR was not evident, suggesting that the transcriptional downregulation mechanism is controlled differently from the UPR... - HacA-independent induction of chaperone-encoding gene bipA in Aspergillus niger strains overproducing membrane proteinsAnoushka Davé
School of Biology, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom
Appl Environ Microbiol 72:953-5. 2006..Despite elevated bipA mRNA levels, no 5'-truncated hacA transcript was detected, raising the possibility of a hacA-independent induction of bipA mRNA under the stress of membrane protein overproduction in A. niger... - Filamentous fungi for production of food additives and processing aidsDavid B Archer
School of Biology, University of Nottingham, University Park, NG7 2RD, Nottingham, UK
Adv Biochem Eng Biotechnol 111:99-147. 2008..In this review chapter we focus on the use of fungi in the production of food additives but take a largely pre-genomic, albeit a mainly molecular, view of the topic... - The effect of variation within inhibitory domains on the activity of pea protease inhibitors from the Bowman-Birk classAlfonso Clemente
Department of Metabolic Biology, John Innes Centre, Norwich Research Park, NR4 7UH, UK
Protein Expr Purif 36:106-14. 2004..Differences in trypsin and chymotrypsin specific inhibitory activities, and in inhibition constants, were observed in studies of the two recombinant variants and BBI...