S Krugmann

Summary

Affiliation: Institute for Animal Health
Country: UK

Publications

  1. ncbi request reprint Characterizing the interactions between the two subunits of the p101/p110gamma phosphoinositide 3-kinase and their role in the activation of this enzyme by G beta gamma subunits
    S Krugmann
    Signalling Programme, The Babraham Institute, Babraham, Cambridge CB2 4AT, United Kingdom
    J Biol Chem 274:17152-8. 1999
  2. ncbi request reprint ARAP3 is a PI3K- and rap-regulated GAP for RhoA
    Sonja Krugmann
    Inositide Laboratory, The Babraham Institute, Cambridge CB2 4AT, United Kingdom
    Curr Biol 14:1380-4. 2004
  3. ncbi request reprint ARAP3 is essential for formation of lamellipodia after growth factor stimulation
    Sonja Krugmann
    The Inositide Laboratory, The Babraham Institute, Cambridge, CB2 4AT, UK
    J Cell Sci 119:425-32. 2006
  4. ncbi request reprint Identification of ARAP3, a novel PI3K effector regulating both Arf and Rho GTPases, by selective capture on phosphoinositide affinity matrices
    S Krugmann
    Inositide Laboratory, The Babraham Institute, Cambridge, CB2 4AT, United Kingdom
    Mol Cell 9:95-108. 2002
  5. ncbi request reprint Purification of ARAP3 and characterization of GAP activities
    Sonja Krugmann
    Inositide Laboratory, The Babraham Institute, Cambridge, UK
    Methods Enzymol 406:91-103. 2006
  6. ncbi request reprint Regulation of P-Rex1 by phosphatidylinositol (3,4,5)-trisphosphate and Gbetagamma subunits
    Kirsti Hill
    Inositide Laboratory, The Babraham Institute, Babraham Research Campus, Cambridge CB2 4AT, United Kingdom
    J Biol Chem 280:4166-73. 2005
  7. ncbi request reprint The PI3K effector Arap3 interacts with the PI(3,4,5)P3 phosphatase SHIP2 in a SAM domain-dependent manner
    Judith H Raaijmakers
    Department of Physiological Chemistry and Centre of Biomedical Genetics, UMC Utrecht, Universiteitsweg 100, 3584 CG Utrecht, The Netherlands
    Cell Signal 19:1249-57. 2007
  8. pmc Mechanism of the regulation of type IB phosphoinositide 3OH-kinase byG-protein betagamma subunits
    Sonja Krugmann
    Signalling Programme, The Babraham Institute, Babraham, Cambridge CB2 4AT, U K
    Biochem J 362:725-31. 2002
  9. ncbi request reprint P-Rex2, a new guanine-nucleotide exchange factor for Rac
    Sarah Donald
    Signalling Programme, Inositide Laboratory, The Babraham Institute, Babraham, Cambridge CB2 4AT, UK
    FEBS Lett 572:172-6. 2004
  10. ncbi request reprint Sphingosine kinase 1 is an intracellular effector of phosphatidic acid
    Christine Delon
    Department of Signalling, Babraham Institute, Cambridge CB2 4AT, United Kingdom
    J Biol Chem 279:44763-74. 2004

Collaborators

Detail Information

Publications10

  1. ncbi request reprint Characterizing the interactions between the two subunits of the p101/p110gamma phosphoinositide 3-kinase and their role in the activation of this enzyme by G beta gamma subunits
    S Krugmann
    Signalling Programme, The Babraham Institute, Babraham, Cambridge CB2 4AT, United Kingdom
    J Biol Chem 274:17152-8. 1999
    ..While modifications of the N terminus of p110gamma could modulate its intrinsic catalytic activity, binding to the N-terminal region of p101 was found to be indispensable for activation of heterodimers with Gbetagamma...
  2. ncbi request reprint ARAP3 is a PI3K- and rap-regulated GAP for RhoA
    Sonja Krugmann
    Inositide Laboratory, The Babraham Institute, Cambridge CB2 4AT, United Kingdom
    Curr Biol 14:1380-4. 2004
    ..Our results indicate that ARAP3 is a Rap-effector that plays an important role in mediating PI3K-dependent crosstalk between Ras, Rho, and Arf family small GTPases...
  3. ncbi request reprint ARAP3 is essential for formation of lamellipodia after growth factor stimulation
    Sonja Krugmann
    The Inositide Laboratory, The Babraham Institute, Cambridge, CB2 4AT, UK
    J Cell Sci 119:425-32. 2006
    ..We conclude that ARAP3 recruitment to sites of elevated PtdIns(3,4,5)P(3) is crucial to allow localised inactivation of RhoA and cycling of Arf6, both of which are necessary to allow growth factor-stimulated formation of lamellipodia...
  4. ncbi request reprint Identification of ARAP3, a novel PI3K effector regulating both Arf and Rho GTPases, by selective capture on phosphoinositide affinity matrices
    S Krugmann
    Inositide Laboratory, The Babraham Institute, Cambridge, CB2 4AT, United Kingdom
    Mol Cell 9:95-108. 2002
    ....
  5. ncbi request reprint Purification of ARAP3 and characterization of GAP activities
    Sonja Krugmann
    Inositide Laboratory, The Babraham Institute, Cambridge, UK
    Methods Enzymol 406:91-103. 2006
    ..This overexpression phenotype depends on ARAP3's intact abilities to bind PtdIns(3,4,5)P3, to interact with Rap-GTP, and to be a catalytically active RhoA and Arf6 GAP...
  6. ncbi request reprint Regulation of P-Rex1 by phosphatidylinositol (3,4,5)-trisphosphate and Gbetagamma subunits
    Kirsti Hill
    Inositide Laboratory, The Babraham Institute, Babraham Research Campus, Cambridge CB2 4AT, United Kingdom
    J Biol Chem 280:4166-73. 2005
    ....
  7. ncbi request reprint The PI3K effector Arap3 interacts with the PI(3,4,5)P3 phosphatase SHIP2 in a SAM domain-dependent manner
    Judith H Raaijmakers
    Department of Physiological Chemistry and Centre of Biomedical Genetics, UMC Utrecht, Universiteitsweg 100, 3584 CG Utrecht, The Netherlands
    Cell Signal 19:1249-57. 2007
    ..Since it was shown previously that Arap3 has a higher affinity for PI(3,4,5,)P3 than for PI(3,4)P2, we propose that the SAM domain of Arap3 can function to recruit a negative regulator of PI3K signaling into the effector complex...
  8. pmc Mechanism of the regulation of type IB phosphoinositide 3OH-kinase byG-protein betagamma subunits
    Sonja Krugmann
    Signalling Programme, The Babraham Institute, Babraham, Cambridge CB2 4AT, U K
    Biochem J 362:725-31. 2002
    ..We conclude that Gbetagammas activate type IB PI3K by a mechanism other than translocation to the plasma membrane...
  9. ncbi request reprint P-Rex2, a new guanine-nucleotide exchange factor for Rac
    Sarah Donald
    Signalling Programme, Inositide Laboratory, The Babraham Institute, Babraham, Cambridge CB2 4AT, UK
    FEBS Lett 572:172-6. 2004
    ..Together, they form an enzyme family capable of mediating Rac signalling downstream of G protein-coupled receptors and phosphoinositide 3-kinase...
  10. ncbi request reprint Sphingosine kinase 1 is an intracellular effector of phosphatidic acid
    Christine Delon
    Department of Signalling, Babraham Institute, Cambridge CB2 4AT, United Kingdom
    J Biol Chem 279:44763-74. 2004
    ..The SK1-PA interaction maps to the C terminus of SK1 and is independent of catalytic activity or of the diacylglycerol kinase-like domain of the enzyme. Thus SK1 constitutes a novel, physiologically relevant PA effector...