P A Kroon
Affiliation: Institute for Animal Health
- A modular esterase from Penicillium funiculosum which releases ferulic acid from plant cell walls and binds crystalline cellulose contains a carbohydrate binding moduleP A Kroon
Institute of Food Research, Norwich Research Park, Colney, Norwich, UK
Eur J Biochem 267:6740-52. 2000..and plants. The C-terminal domain comprised 39 amino acids and closely resembled the family 1 cellulose binding carbohydrate-binding modules (CBM) of fungal glycosyl hydrolases. This is the first report of a fungal CE with a CBM...
- How should we assess the effects of exposure to dietary polyphenols in vitro?Paul A Kroon
Nutrition Division, Institute of Food Research, Norwich, United Kingdom
Am J Clin Nutr 80:15-21. 2004..1-10 micromol/L. For correct interpretation of results, future efforts to define biological activities of polyphenols must make use of the available data concerning bioavailability and metabolism in humans...
- A cinnamoyl esterase from Aspergillus niger can break plant cell wall cross-links without release of free diferulic acidsM T Garcia-Conesa
Institute of Food Research, Norwich Research Park, Colney, UK
Eur J Biochem 266:644-52. 1999....
- Dietary flavonoid and isoflavone glycosides are hydrolysed by the lactase site of lactase phlorizin hydrolaseA J Day
Diet, Health and Consumer Science Division, Institute of Food Research, Norwich Research Park, Colney, Norwich, UK
FEBS Lett 468:166-70. 2000..The ability of LPH to deglycosylate dietary (iso)flavonoid glycosides suggests a possible role for this enzyme in the metabolism of these biologically active compounds...
- Influence of ferulic acid on the production of feruloyl esterases by Aspergillus nigerC B Faulds
Department of Biochemistry, Institute of Food Research, Colney, Norwich, UK
FEMS Microbiol Lett 157:239-44. 1997..However, production is stimulated by the presence of free ferulic acid through increased expression, and is reduced by the removal of esterified ferulic acid from the growth substrate...
- Human O-sulfated metabolites of (-)-epicatechin and methyl-(-)-epicatechin are poor substrates for commercial aryl-sulfatases: implications for studies concerned with quantifying epicatechin bioavailabilityS Saha
Institute of Food Research, Norwich Research Park, Norwich NR4 7UA, UK
Pharmacol Res 65:592-602. 2012..Methods for quantifying epicatechin concentrations in blood and urine need to take account of the lack of hydrolysis of (methyl)epicatechin-sulfates, for example by quantifying these directly using LC-MS/MS...
- Activity of an Aspergillus terreus alpha-arabinofuranosidase on phenolic-substituted oligosaccharidesE Luonteri
Biochemistry Department, Norwich Research Park, UK
J Biotechnol 67:41-8. 1999..Somewhat surprisingly, the LCC-model compound, in which the arabinose residue is substituted with a bulky dilignol group, was degraded by the enzyme. This indicated that the enzyme is able to approach this linkage from the xylose side...
- Esterase activity able to hydrolyze dietary antioxidant hydroxycinnamates is distributed along the intestine of mammalsM F Andreasen
Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, United Kingdom
J Agric Food Chem 49:5679-84. 2001..Hydrolysis by intestinal esterase(s) is very likely the major route for release of antioxidant hydroxycinnamic acids in vivo...
- An Aspergillus niger esterase (ferulic acid esterase III) and a recombinant Pseudomonas fluorescens subsp. cellulosa esterase (Xy1D) release a 5-5' ferulic dehydrodimer (diferulic acid) from barley and wheat cell wallsB Bartolome
Department of Biochemistry, Institute of Food Research, Colney, Norwich, United Kingdom
Appl Environ Microbiol 63:208-12. 1997..It is also concluded that the esterified dimer content of the xylanase-solubilized fraction depends on the source of the xylanase...
- Absorption of kaempferol from endive, a source of kaempferol-3-glucuronide, in humansM S DuPont
Institute of Food Research, Norwich Research Park, Colney Lane, Norwich, UK
Eur J Clin Nutr 58:947-54. 2004..The predominant form in plasma is a 3-glucuronide conjugate, and interindividual variation in absorption and excretion is low, suggesting that urinary kaempferol could be used as a biomarker for exposure...
- The procyanidin-mediated induction of apoptosis and cell-cycle arrest in esophageal adenocarcinoma cells is not dependent on p21(Cip1/WAF1)R Pierini
Institute of Food Research, Norwich Research Park, Colney Lane, Norwich NR4 7UA, UK
Cancer Lett 270:234-41. 2008..However, blocking the PA-induced up-regulation of p21 expression with siRNA did not alter PA-mediated changes in apoptosis and cell cycle, demonstrating that p21 is not responsible for the PA-induced effects...
- Intestinal release and uptake of phenolic antioxidant diferulic acidsM F Andreasen
Department of Horticulture, Danish Institute of Agricultural Sciences, Aarslev, Denmark
Free Radic Biol Med 31:304-14. 2001..Our results suggest that the phenolic antioxidant diferulic acids are bioavailable...