Richard K Hughes

Summary

Affiliation: Institute for Animal Health
Country: UK

Publications

  1. ncbi Subcellular localisation of Medicago truncatula 9/13-hydroperoxide lyase reveals a new localisation pattern and activation mechanism for CYP74C enzymes
    Stefania De Domenico
    Institute of Sciences of Food Production C N R Section of Lecce, Via Monteroni, 73100, Lecce, Italy
    BMC Plant Biol 7:58. 2007
  2. ncbi Plant cytochrome CYP74 family: biochemical features, endocellular localisation, activation mechanism in plant defence and improvements for industrial applications
    Richard K Hughes
    John Innes Centre, Norwich Research Park, Norwich, Norfolk NR4 7UH, UK
    Chembiochem 10:1122-33. 2009
  3. ncbi Evidence for communality in the primary determinants of CYP74 catalysis and of structural similarities between CYP74 and classical mammalian P450 enzymes
    Richard K Hughes
    John Innes Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom
    Proteins 72:1199-211. 2008
  4. ncbi Mutagenesis and modelling of linoleate-binding to pea seed lipoxygenase
    R K Hughes
    John Innes Centre, Norwich Research Park, Norwich, UK
    Eur J Biochem 268:1030-40. 2001
  5. ncbi CYP74C3 and CYP74A1, plant cytochrome P450 enzymes whose activity is regulated by detergent micelle association, and proposed new rules for the classification of CYP74 enzymes
    R K Hughes
    John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK
    Biochem Soc Trans 34:1223-7. 2006
  6. ncbi Allene oxide synthase from Arabidopsis thaliana (CYP74A1) exhibits dual specificity that is regulated by monomer-micelle association
    Richard K Hughes
    John Innes Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom
    FEBS Lett 580:4188-94. 2006
  7. ncbi Characterization of Medicago truncatula (barrel medic) hydroperoxide lyase (CYP74C3), a water-soluble detergent-free cytochrome P450 monomer whose biological activity is defined by monomer-micelle association
    Richard K Hughes
    John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK
    Biochem J 395:641-52. 2006
  8. ncbi The gateway pDEST17 expression vector encodes a -1 ribosomal frameshifting sequence
    Eric J Belfield
    John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK
    Nucleic Acids Res 35:1322-32. 2007
  9. ncbi Distinct regions of the Pseudomonas syringae coiled-coil effector AvrRps4 are required for activation of immunity
    Kee Hoon Sohn
    The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, United Kingdom
    Proc Natl Acad Sci U S A 109:16371-6. 2012
  10. ncbi Glycosyltransferases from oat (Avena) implicated in the acylation of avenacins
    Amorn Owatworakit
    From the Department of Metabolic Biology and
    J Biol Chem 288:3696-704. 2013

Collaborators

Detail Information

Publications13

  1. ncbi Subcellular localisation of Medicago truncatula 9/13-hydroperoxide lyase reveals a new localisation pattern and activation mechanism for CYP74C enzymes
    Stefania De Domenico
    Institute of Sciences of Food Production C N R Section of Lecce, Via Monteroni, 73100, Lecce, Italy
    BMC Plant Biol 7:58. 2007
    ....
  2. ncbi Plant cytochrome CYP74 family: biochemical features, endocellular localisation, activation mechanism in plant defence and improvements for industrial applications
    Richard K Hughes
    John Innes Centre, Norwich Research Park, Norwich, Norfolk NR4 7UH, UK
    Chembiochem 10:1122-33. 2009
    ..In this review we focus on new data relating to the physiological significance of different phyto-oxylipins and related enzymes. Moreover, recent advances in the biotechnological production of oxylipins are also discussed...
  3. ncbi Evidence for communality in the primary determinants of CYP74 catalysis and of structural similarities between CYP74 and classical mammalian P450 enzymes
    Richard K Hughes
    John Innes Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom
    Proteins 72:1199-211. 2008
    ..We suggest that the overall structure of CYP74 enzymes is likely to be very similar to those described for classical P450 monooxygenase enzymes...
  4. ncbi Mutagenesis and modelling of linoleate-binding to pea seed lipoxygenase
    R K Hughes
    John Innes Centre, Norwich Research Park, Norwich, UK
    Eur J Biochem 268:1030-40. 2001
    ..A single model for positional specificity, that has proved to be inappropriate for arachidonate-binding to mammalian 5-, 12- and 15-lipoxygenases, would appear to be true also for linoleate-binding to plant 9- and 13-lipoxygenases...
  5. ncbi CYP74C3 and CYP74A1, plant cytochrome P450 enzymes whose activity is regulated by detergent micelle association, and proposed new rules for the classification of CYP74 enzymes
    R K Hughes
    John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK
    Biochem Soc Trans 34:1223-7. 2006
    ....
  6. ncbi Allene oxide synthase from Arabidopsis thaliana (CYP74A1) exhibits dual specificity that is regulated by monomer-micelle association
    Richard K Hughes
    John Innes Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom
    FEBS Lett 580:4188-94. 2006
    ..CYP74A1 may be suited for structural resolution of the first plant cytochrome P450 and its 9-AOS activity and behaviour in vitro has implications for its role in planta...
  7. ncbi Characterization of Medicago truncatula (barrel medic) hydroperoxide lyase (CYP74C3), a water-soluble detergent-free cytochrome P450 monomer whose biological activity is defined by monomer-micelle association
    Richard K Hughes
    John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK
    Biochem J 395:641-52. 2006
    ..Highly concentrated and monodispersed samples of detergent-free CYP74C3 protein may be well suited for the purposes of crystallization and structural resolution of the first plant cytochrome P450 enzyme...
  8. ncbi The gateway pDEST17 expression vector encodes a -1 ribosomal frameshifting sequence
    Eric J Belfield
    John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK
    Nucleic Acids Res 35:1322-32. 2007
    ..These findings suggest that caution should be exercised when employing pDEST17 for high-level protein expression and that the attB1 site has some potential as a tool for studying -1 frameshifting...
  9. ncbi Distinct regions of the Pseudomonas syringae coiled-coil effector AvrRps4 are required for activation of immunity
    Kee Hoon Sohn
    The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, United Kingdom
    Proc Natl Acad Sci U S A 109:16371-6. 2012
    ..The structure of AvrRps4(C), and identification of functionally important residues for its activation of plant immunity, advances our understanding of these processes in a well-defined model pathosystem...
  10. ncbi Glycosyltransferases from oat (Avena) implicated in the acylation of avenacins
    Amorn Owatworakit
    From the Department of Metabolic Biology and
    J Biol Chem 288:3696-704. 2013
    ....
  11. ncbi Functional identification of the cDNA coding for a wheat endo-1,4-beta-D-xylanase inhibitor
    Giles O Elliott
    Institute of Food Research, Norwich Research Park, Norwich, UK
    FEBS Lett 519:66-70. 2002
    ..This is the first full-length cDNA sequence that encodes a novel class of protein which inhibits the activity of endo-1,4-beta-D-xylanases...
  12. ncbi A serine carboxypeptidase-like acyltransferase is required for synthesis of antimicrobial compounds and disease resistance in oats
    Sam T Mugford
    John Ines Centre, Norwich NR4 7UH, United Kingdom
    Plant Cell 21:2473-84. 2009
    ..Oat SCPL1 (SAD7) is the founder member of a subfamily of monocot-specific SCPL proteins that includes predicted proteins from rice (Oryza sativa) and other grasses with potential roles in secondary metabolism and plant defense...
  13. ncbi The molecular basis of ubiquitin-like protein NEDD8 deamidation by the bacterial effector protein Cif
    Allister Crow
    Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom
    Proc Natl Acad Sci U S A 109:E1830-8. 2012
    ..We used native PAGE to follow the activity of Cif from the human pathogen Yersinia pseudotuberculosis and selected variants, and the position of Gln40 in the active site has allowed us to propose a catalytic mechanism for these enzymes...