Richard K Hughes

Summary

Affiliation: Institute for Animal Health
Country: UK

Publications

  1. pmc Subcellular localisation of Medicago truncatula 9/13-hydroperoxide lyase reveals a new localisation pattern and activation mechanism for CYP74C enzymes
    Stefania De Domenico
    Institute of Sciences of Food Production C N R Section of Lecce, Via Monteroni, 73100, Lecce, Italy
    BMC Plant Biol 7:58. 2007
  2. ncbi request reprint Mutagenesis and modelling of linoleate-binding to pea seed lipoxygenase
    R K Hughes
    John Innes Centre, Norwich Research Park, Norwich, UK
    Eur J Biochem 268:1030-40. 2001
  3. doi request reprint Plant cytochrome CYP74 family: biochemical features, endocellular localisation, activation mechanism in plant defence and improvements for industrial applications
    Richard K Hughes
    John Innes Centre, Norwich Research Park, Norwich, Norfolk NR4 7UH, UK
    Chembiochem 10:1122-33. 2009
  4. doi request reprint Evidence for communality in the primary determinants of CYP74 catalysis and of structural similarities between CYP74 and classical mammalian P450 enzymes
    Richard K Hughes
    John Innes Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom
    Proteins 72:1199-211. 2008
  5. ncbi request reprint CYP74C3 and CYP74A1, plant cytochrome P450 enzymes whose activity is regulated by detergent micelle association, and proposed new rules for the classification of CYP74 enzymes
    R K Hughes
    John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK
    Biochem Soc Trans 34:1223-7. 2006
  6. ncbi request reprint Allene oxide synthase from Arabidopsis thaliana (CYP74A1) exhibits dual specificity that is regulated by monomer-micelle association
    Richard K Hughes
    John Innes Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom
    FEBS Lett 580:4188-94. 2006
  7. pmc Characterization of Medicago truncatula (barrel medic) hydroperoxide lyase (CYP74C3), a water-soluble detergent-free cytochrome P450 monomer whose biological activity is defined by monomer-micelle association
    Richard K Hughes
    John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK
    Biochem J 395:641-52. 2006
  8. pmc The gateway pDEST17 expression vector encodes a -1 ribosomal frameshifting sequence
    Eric J Belfield
    John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK
    Nucleic Acids Res 35:1322-32. 2007
  9. pmc Distinct regions of the Pseudomonas syringae coiled-coil effector AvrRps4 are required for activation of immunity
    Kee Hoon Sohn
    The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, United Kingdom
    Proc Natl Acad Sci U S A 109:16371-6. 2012
  10. doi request reprint The X-ray crystal structure of APR-B, an atypical adenosine 5'-phosphosulfate reductase from Physcomitrella patens
    Clare E M Stevenson
    Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK
    FEBS Lett 587:3626-32. 2013

Collaborators

Detail Information

Publications16

  1. pmc Subcellular localisation of Medicago truncatula 9/13-hydroperoxide lyase reveals a new localisation pattern and activation mechanism for CYP74C enzymes
    Stefania De Domenico
    Institute of Sciences of Food Production C N R Section of Lecce, Via Monteroni, 73100, Lecce, Italy
    BMC Plant Biol 7:58. 2007
    ....
  2. ncbi request reprint Mutagenesis and modelling of linoleate-binding to pea seed lipoxygenase
    R K Hughes
    John Innes Centre, Norwich Research Park, Norwich, UK
    Eur J Biochem 268:1030-40. 2001
    ..A single model for positional specificity, that has proved to be inappropriate for arachidonate-binding to mammalian 5-, 12- and 15-lipoxygenases, would appear to be true also for linoleate-binding to plant 9- and 13-lipoxygenases...
  3. doi request reprint Plant cytochrome CYP74 family: biochemical features, endocellular localisation, activation mechanism in plant defence and improvements for industrial applications
    Richard K Hughes
    John Innes Centre, Norwich Research Park, Norwich, Norfolk NR4 7UH, UK
    Chembiochem 10:1122-33. 2009
    ..In this review we focus on new data relating to the physiological significance of different phyto-oxylipins and related enzymes. Moreover, recent advances in the biotechnological production of oxylipins are also discussed...
  4. doi request reprint Evidence for communality in the primary determinants of CYP74 catalysis and of structural similarities between CYP74 and classical mammalian P450 enzymes
    Richard K Hughes
    John Innes Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom
    Proteins 72:1199-211. 2008
    ..We suggest that the overall structure of CYP74 enzymes is likely to be very similar to those described for classical P450 monooxygenase enzymes...
  5. ncbi request reprint CYP74C3 and CYP74A1, plant cytochrome P450 enzymes whose activity is regulated by detergent micelle association, and proposed new rules for the classification of CYP74 enzymes
    R K Hughes
    John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK
    Biochem Soc Trans 34:1223-7. 2006
    ....
  6. ncbi request reprint Allene oxide synthase from Arabidopsis thaliana (CYP74A1) exhibits dual specificity that is regulated by monomer-micelle association
    Richard K Hughes
    John Innes Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom
    FEBS Lett 580:4188-94. 2006
    ..CYP74A1 may be suited for structural resolution of the first plant cytochrome P450 and its 9-AOS activity and behaviour in vitro has implications for its role in planta...
  7. pmc Characterization of Medicago truncatula (barrel medic) hydroperoxide lyase (CYP74C3), a water-soluble detergent-free cytochrome P450 monomer whose biological activity is defined by monomer-micelle association
    Richard K Hughes
    John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK
    Biochem J 395:641-52. 2006
    ..Highly concentrated and monodispersed samples of detergent-free CYP74C3 protein may be well suited for the purposes of crystallization and structural resolution of the first plant cytochrome P450 enzyme...
  8. pmc The gateway pDEST17 expression vector encodes a -1 ribosomal frameshifting sequence
    Eric J Belfield
    John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK
    Nucleic Acids Res 35:1322-32. 2007
    ..These findings suggest that caution should be exercised when employing pDEST17 for high-level protein expression and that the attB1 site has some potential as a tool for studying -1 frameshifting...
  9. pmc Distinct regions of the Pseudomonas syringae coiled-coil effector AvrRps4 are required for activation of immunity
    Kee Hoon Sohn
    The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, United Kingdom
    Proc Natl Acad Sci U S A 109:16371-6. 2012
    ..The structure of AvrRps4(C), and identification of functionally important residues for its activation of plant immunity, advances our understanding of these processes in a well-defined model pathosystem...
  10. doi request reprint The X-ray crystal structure of APR-B, an atypical adenosine 5'-phosphosulfate reductase from Physcomitrella patens
    Clare E M Stevenson
    Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK
    FEBS Lett 587:3626-32. 2013
    ..Structural conservation with bacterial APS reductase rules out a structural role for the cluster, but supports the contention that it enhances the activity of conventional APS reductases. ..
  11. pmc Biochemical analysis of a multifunctional cytochrome P450 (CYP51) enzyme required for synthesis of antimicrobial triterpenes in plants
    Katrin Geisler
    Department of Metabolic Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom
    Proc Natl Acad Sci U S A 110:E3360-7. 2013
    ....
  12. pmc Glycosyltransferases from oat (Avena) implicated in the acylation of avenacins
    Amorn Owatworakit
    Department of Metabolic Biology, The John Innes Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom
    J Biol Chem 288:3696-704. 2013
    ....
  13. ncbi request reprint Functional identification of the cDNA coding for a wheat endo-1,4-beta-D-xylanase inhibitor
    Giles O Elliott
    Institute of Food Research, Norwich Research Park, Norwich, UK
    FEBS Lett 519:66-70. 2002
    ..This is the first full-length cDNA sequence that encodes a novel class of protein which inhibits the activity of endo-1,4-beta-D-xylanases...
  14. pmc A serine carboxypeptidase-like acyltransferase is required for synthesis of antimicrobial compounds and disease resistance in oats
    Sam T Mugford
    John Ines Centre, Norwich NR4 7UH, United Kingdom
    Plant Cell 21:2473-84. 2009
    ..Oat SCPL1 (SAD7) is the founder member of a subfamily of monocot-specific SCPL proteins that includes predicted proteins from rice (Oryza sativa) and other grasses with potential roles in secondary metabolism and plant defense...
  15. doi request reprint Production of RXLR effector proteins for structural analysis by X-ray crystallography
    Richard K Hughes
    Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK
    Methods Mol Biol 1127:231-53. 2014
    ..coli has proven difficult, or where there is a desire to evaluate E. coli thoroughly as a host before considering alternative hosts for heterologous expression. ..
  16. pmc The molecular basis of ubiquitin-like protein NEDD8 deamidation by the bacterial effector protein Cif
    Allister Crow
    Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom
    Proc Natl Acad Sci U S A 109:E1830-8. 2012
    ..We used native PAGE to follow the activity of Cif from the human pathogen Yersinia pseudotuberculosis and selected variants, and the position of Gln40 in the active site has allowed us to propose a catalytic mechanism for these enzymes...