Research Topics
Genomes and Genes | Jonathan HouseleySummaryAffiliation: Institute for Animal Health Country: UK Publications
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Publications
Repeat expansion in the budding yeast ribosomal DNA can occur independently of the canonical homologous recombination machineryJonathan Houseley
Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, EH9 3JR, UK
Nucleic Acids Res 39:8778-91. 2011..Our data suggest that a novel mode of break-induced replication occurs in repetitive DNA that is dependent on high homology but does not require the canonical homologous recombination machinery...
Apparent non-canonical trans-splicing is generated by reverse transcriptase in vitroJonathan Houseley
Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, United Kingdom
PLoS ONE 5:e12271. 2010..Particularly, the native ability of reverse transcriptase enzymes to template switch during transcription could produce apparently trans-spliced sequences...
Trf4 targets ncRNAs from telomeric and rDNA spacer regions and functions in rDNA copy number controlJonathan Houseley
Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, UK
EMBO J 26:4996-5006. 2007..Co-transcriptional RNA binding by Trf4 may link RNA and DNA metabolism and direct immediate IGS1-R degradation by the exosome following transcription termination...
Yeast Trf5p is a nuclear poly(A) polymeraseJonathan Houseley
Wellcome Trust Centre for Cell Biology, University of Edinburgh, Swann Building, King s Buildings, Edinburgh EH9 3JR, UK
EMBO Rep 7:205-11. 2006..Trf5p co-purified with Mtr4p and Air1p, indicating that it forms a complex, designated TRAMP5, that has functions that partially overlap with the TRAMP complex...
Surveillance of nuclear-restricted pre-ribosomes within a subnucleolar region of Saccharomyces cerevisiaeChristophe Dez
Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, UK
EMBO J 25:1534-46. 2006..Localization of pre-ribosomes to this focus was lost in sda1-2 strains lacking Trf4p or Rrp6p. We designate this nucleolar focus the No-body and propose that it represents a site of pre-ribosome surveillance...
A ncRNA modulates histone modification and mRNA induction in the yeast GAL gene clusterJonathan Houseley
Wellcome Trust Centre for Cell Biology, Institute for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, UK
Mol Cell 32:685-95. 2008..These chromatin modifications act principally through the Rpd3S complex to aid glucose repression of GAL1-10 at physiologically relevant sugar concentrations...
The nuclear RNA surveillance machinery: the link between ncRNAs and genome structure in budding yeast?Jonathan Houseley
Wellcome Trust Centre for Cell Biology, University of Edinburgh, UK
Biochim Biophys Acta 1779:239-46. 2008..Less well-understood observations link both the TRAMP and exosome complexes to chromatin structure and DNA repair, and we will speculate on the potential significance of these activities...
RNA-quality control by the exosomeJonathan Houseley
Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, EH9 3JR, UK
Nat Rev Mol Cell Biol 7:529-39. 2006..Recent studies now provide insights into the regulation and structure of the exosome, and they reveal striking similarities between the process of RNA degradation in bacteria and eukaryotes...
The many pathways of RNA degradationJonathan Houseley
Wellcome Trust Centre for Cell Biology, University of Edinburgh, EH9 3JR, UK
Cell 136:763-76. 2009..Because RNA degradation is ubiquitous in all cells, it is clear that it must be carefully controlled to accurately recognize target RNAs. How this is achieved is perhaps the most pressing question in the field...
RNA degradation by the exosome is promoted by a nuclear polyadenylation complexJohn LaCava
Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, UK
Cell 121:713-24. 2005..We speculate that this function was maintained in eukaryotic nuclei, while cytoplasmic mRNA poly(A) tails acquired different roles in translation...
