Research Topics
| M HeSummaryAffiliation: Institute for Animal Health Country: UK Publications
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Detail Information
Publications
Antibody-ribosome-mRNA (ARM) complexes as efficient selection particles for in vitro display and evolution of antibody combining sitesM He
Laboratory of Molecular Recognition and Translocus Therapeutics Ltd, The Babraham Institute, Babraham, Cambridge CB2 4AT, UK
Nucleic Acids Res 25:5132-4. 1997..While demonstrated here for antibodies, the method has the potential to be applied equally for selection of receptors or peptides from libraries...
Rapid discovery of protein interactions by cell-free protein technologiesM He
Technology Research Group, The Babraham Institute, Cambridge CB22 3AT, U K
Biochem Soc Trans 35:962-5. 2007..These technologies can be combined for rapid detection of protein interactions...
In situ synthesis of protein arraysMingyue He
Technology Research Group, The Babraham Institute, Cambridge CB22 3AT, UK
Curr Opin Biotechnol 19:4-9. 2008..Recently employed methods reviewed are PISA (protein in situ array) and NAPPA (nucleic acid programmable protein array) from DNA and puromycin-mediated immobilisation from mRNA...- Cell-free protein synthesis: applications in proteomics and biotechnologyMingyue He
The Babraham Institute, Babraham Research Campus, Cambridge CB22 3AT, UK
N Biotechnol 25:126-32. 2008..This article reviews the recent development in cell-free systems and their application in the large scale protein analysis... - In vitro protein expression: an emerging alternative to cell-based approachesMingyue He
Protein Expression Facility, The Babraham Institute, Babraham Research Campus, Cambridge CB22 3AT, UK
N Biotechnol 28:209-10. 2011..This special issue of New Biotechnology describes recent advances in cell-free protein expression systems and their applications... - Detection of protein-protein interactions by ribosome display and protein in situ immobilisationMingyue He
The Babraham Institute, Babraham Research Campus, Cambridge CB22 3AT, UK
N Biotechnol 26:277-81. 2009..The method has promise for library screening of pairwise protein interactions, down to the analytical level of individual domain or motif mapping... - Arraying proteins by cell-free synthesisMingyue He
Technology Research Group, The Babraham Institute, Cambridge CB22 3AT, United Kingdom
Biomol Eng 24:375-80. 2007..This article reviews current cell-free protein array technologies and their proteomic applications... - Eukaryotic ribosome display with in situ DNA recoveryMingyue He
The Inositide Laboratory, The Babraham Institute, Cambridge, UK
Methods Mol Biol 805:75-85. 2012.... - Effects of mutation at the D-JH junction on affinity, specificity, and idiotypy of anti-progesterone antibody DB3Mingyue He
Technology Research Group, The Babraham Institute, Cambridge, United Kingdom
Protein Sci 15:2141-8. 2006.... - Advancing protein therapeutics: current challenges in protein therapeutics -- CHI's Second Annual ConferenceMingyue He
The Babraham Institute, Babraham Research Campus, Cambridge CB2 4AT, UK
IDrugs 9:177-8. 2006 - Single step generation of protein arrays from DNA by cell-free expression and in situ immobilisation (PISA method)M He
Technology Research Group, The Babraham Institute, Babraham, Cambridge CB2 4AT, UK
Nucleic Acids Res 29:E73-3. 2001..Here we show that human single-chain antibody fragments (three domain, V(H)/K form) and an enzyme (luciferase) can be functionally arrayed by the PISA method... - Ribosome display: next-generation display technologies for production of antibodies in vitroMingyue He
Protein Technologies Laboratory, Babraham Research Campus, Cambridge, CB2 4AT, UK
Expert Rev Proteomics 2:421-30. 2005..In the near future, cell-free protein synthesis may be developed as an alternative for large-scale generation of antibodies... - DiscernArray technology: a cell-free method for the generation of protein arrays from PCR DNAMingyue He
Discerna Limited, Babraham Research Campus, Babraham, CB2 4AT, Cambridge, UK
J Immunol Methods 274:265-70. 2003..DiscernArray is particularly useful for arraying proteins and domains which cannot be functionally produced in heterologous expression systems or for which the cloned DNA is not available... - Ribosome display of antibodies: expression, specificity and recovery in a eukaryotic systemMingyue He
Protein Technologies Laboratory, The Babraham Institute, The Babraham Research Campus, Cambridge CB2 4AT, UK
J Immunol Methods 297:73-82. 2005..Our findings confirm the effectiveness of the eukaryotic ribosome display system and define conditions for efficient selection of single chain antibodies... - Generation of protein in situ arrays by DiscernArray technologyMingyue He
Discerna Limited, Cambridge, UK
Methods Mol Biol 264:25-31. 2004..Protein arrays of single-chain antibodies, ligand-binding domains, and enzymes have been successfully generated by this method... - Cell-free protein synthesis for proteomicsAlison M Jackson
Discerna Ltd, Babraham Research Campus, Babraham, Cambridge CB2 4AT, UK
Brief Funct Genomic Proteomic 2:308-19. 2004..This paper reviews the most commonly used cell-free systems and their applications in proteomics... - Protein microarrays: high-throughput tools for proteomicsOda Stoevesandt
Babraham Bioscience Technologies Ltd, Babraham Research Campus, Cambridge, CB22 3AT, UK
Expert Rev Proteomics 6:145-57. 2009..This article reviews recent developments in the generation of protein microarrays and their applications in proteomics and diagnostics... - Selection of recombinant antibodies by eukaryotic ribosome displayMingyue He
Technology Research Group, The Babraham Institute, Cambridge, UK
Methods Mol Biol 484:193-205. 2008..We describe our eukaryotic system, in which rabbit reticulocyte extracts are used for cell free transcription/translation and cDNA is recovered by in situ RT-PCR performed on the selected complexes... - Eukaryotic ribosome display with in situ DNA recoveryMingyue He
Technology Research Group, The Babraham Institute, Cambridge CB22 4AT, UK
Nat Methods 4:281-8. 2007..We also introduce a recent, previously unpublished improvement to the procedure in which in situ reverse transcription is combined with sensitive single-primer PCR technology... - Enhanced cell-free protein expression by fusion with immunoglobulin Ckappa domainElizabeth Palmer
Technology Research Group, The Babraham Institute, Cambridge CB2 4AT, United Kingdom
Protein Sci 15:2842-6. 2006.... - In situ biosynthesis of peptide arraysMingyue He
The Babraham Institute, Cambridge, UK
Methods Mol Biol 615:345-56. 2010..Our methods allow making polypeptide arrays using amplified linear DNA fragments... - Production of protein arrays by cell-free systemsMingyue He
Technology Research Group, The Babraham Institute, Cambridge, UK
Methods Mol Biol 484:207-15. 2008..Advantages of PISA include avoiding bacterial expression and protein purification and making functional protein arrays available as required from genetic information... - Production of human single-chain antibodies by ribosome displayMingyue He
Discerna Ltd, Babraham Hall, Babraham, Cambridge, UK
Methods Mol Biol 248:177-89. 2004 - Printing protein arrays from DNA arraysMingyue He
Technology Research Group, The Babraham Institute, Cambridge CB22 3AT, UK
Nat Methods 5:175-7. 2008..DAPA eliminates the need for separate protein expression, purification and spotting, and also overcomes the problem of long-term functional storage of surface-bound proteins... - Ribosome display: cell-free protein display technologyMingyue He
Discerna Limited, Babraham, Cambridge, UK
Brief Funct Genomic Proteomic 1:204-12. 2002..Both prokaryotic and eukaryotic ribosome display systems have been developed and each has its own distinctive features. In this paper, ribosome display systems and their application in selection and evolution of proteins are reviewed... - Double-hexahistidine tag with high-affinity binding for protein immobilization, purification, and detection on ni-nitrilotriacetic acid surfacesFarid Khan
Protein Technologies Laboratory, The Babraham Institute, Babraham Research Campus, Cambridge CB2 4AT, UK
Anal Chem 78:3072-9. 2006..The double-His6 peptide has the potential to be a universal tag for protein immobilization and detection on arrays and single-step purification of proteins from crude mixtures... - The rapid discovery of engineered antibodiesMingrong Wang
Centre for Protein Therapeutics, Sichuan Industrial Institute of Antibiotics, Sichuan 610051, China
IDrugs 10:562-5. 2007..The combination of ribosome display, selection and cell-free screening provides a rapid discovery tool for the generation, optimization and modification of antibody-combining sites... - Selection of antigenic markers on a GFP-Ckappa fusion scaffold with high sensitivity by eukaryotic ribosome displayYong Min Yang
The Institute of Genetics, San Diego, CA 92121 2233, USA
Biochem Biophys Res Commun 359:251-7. 2007..This platform is highly specific and sensitive for the antigen-antibody interaction and may permit selection and reshaping of high affinity antigenic variants of scaffold proteins... - Improved affinity coupling for antibody microarrays: engineering of double-(His)6-tagged single framework recombinant antibody fragmentsCornelia Steinhauer
Department of Immunotechnology, Lund University, Lund, Sweden
Proteomics 6:4227-34. 2006..Taken together, the results demonstrate the generic potential of double-(His)(6)-tag recombinant antibodies for the facile fabrication of high-density antibody microarrays...