M He

Summary

Affiliation: Institute for Animal Health
Country: UK

Publications

  1. pmc Antibody-ribosome-mRNA (ARM) complexes as efficient selection particles for in vitro display and evolution of antibody combining sites
    M He
    Laboratory of Molecular Recognition and Translocus Therapeutics Ltd, The Babraham Institute, Babraham, Cambridge CB2 4AT, UK
    Nucleic Acids Res 25:5132-4. 1997
  2. ncbi request reprint Rapid discovery of protein interactions by cell-free protein technologies
    M He
    Technology Research Group, The Babraham Institute, Cambridge CB22 3AT, U K
    Biochem Soc Trans 35:962-5. 2007
  3. doi request reprint In situ synthesis of protein arrays
    Mingyue He
    Technology Research Group, The Babraham Institute, Cambridge CB22 3AT, UK
    Curr Opin Biotechnol 19:4-9. 2008
  4. doi request reprint Cell-free protein synthesis: applications in proteomics and biotechnology
    Mingyue He
    The Babraham Institute, Babraham Research Campus, Cambridge CB22 3AT, UK
    N Biotechnol 25:126-32. 2008
  5. doi request reprint In vitro protein expression: an emerging alternative to cell-based approaches
    Mingyue He
    Protein Expression Facility, The Babraham Institute, Babraham Research Campus, Cambridge CB22 3AT, UK
    N Biotechnol 28:209-10. 2011
  6. doi request reprint Detection of protein-protein interactions by ribosome display and protein in situ immobilisation
    Mingyue He
    The Babraham Institute, Babraham Research Campus, Cambridge CB22 3AT, UK
    N Biotechnol 26:277-81. 2009
  7. ncbi request reprint Arraying proteins by cell-free synthesis
    Mingyue He
    Technology Research Group, The Babraham Institute, Cambridge CB22 3AT, United Kingdom
    Biomol Eng 24:375-80. 2007
  8. doi request reprint Eukaryotic ribosome display with in situ DNA recovery
    Mingyue He
    The Inositide Laboratory, The Babraham Institute, Cambridge, UK
    Methods Mol Biol 805:75-85. 2012
  9. pmc Effects of mutation at the D-JH junction on affinity, specificity, and idiotypy of anti-progesterone antibody DB3
    Mingyue He
    Technology Research Group, The Babraham Institute, Cambridge, United Kingdom
    Protein Sci 15:2141-8. 2006
  10. ncbi request reprint Advancing protein therapeutics: current challenges in protein therapeutics -- CHI's Second Annual Conference
    Mingyue He
    The Babraham Institute, Babraham Research Campus, Cambridge CB2 4AT, UK
    IDrugs 9:177-8. 2006

Collaborators

Detail Information

Publications29

  1. pmc Antibody-ribosome-mRNA (ARM) complexes as efficient selection particles for in vitro display and evolution of antibody combining sites
    M He
    Laboratory of Molecular Recognition and Translocus Therapeutics Ltd, The Babraham Institute, Babraham, Cambridge CB2 4AT, UK
    Nucleic Acids Res 25:5132-4. 1997
    ..While demonstrated here for antibodies, the method has the potential to be applied equally for selection of receptors or peptides from libraries...
  2. ncbi request reprint Rapid discovery of protein interactions by cell-free protein technologies
    M He
    Technology Research Group, The Babraham Institute, Cambridge CB22 3AT, U K
    Biochem Soc Trans 35:962-5. 2007
    ..These technologies can be combined for rapid detection of protein interactions...
  3. doi request reprint In situ synthesis of protein arrays
    Mingyue He
    Technology Research Group, The Babraham Institute, Cambridge CB22 3AT, UK
    Curr Opin Biotechnol 19:4-9. 2008
    ..Recently employed methods reviewed are PISA (protein in situ array) and NAPPA (nucleic acid programmable protein array) from DNA and puromycin-mediated immobilisation from mRNA...
  4. doi request reprint Cell-free protein synthesis: applications in proteomics and biotechnology
    Mingyue He
    The Babraham Institute, Babraham Research Campus, Cambridge CB22 3AT, UK
    N Biotechnol 25:126-32. 2008
    ..This article reviews the recent development in cell-free systems and their application in the large scale protein analysis...
  5. doi request reprint In vitro protein expression: an emerging alternative to cell-based approaches
    Mingyue He
    Protein Expression Facility, The Babraham Institute, Babraham Research Campus, Cambridge CB22 3AT, UK
    N Biotechnol 28:209-10. 2011
    ..This special issue of New Biotechnology describes recent advances in cell-free protein expression systems and their applications...
  6. doi request reprint Detection of protein-protein interactions by ribosome display and protein in situ immobilisation
    Mingyue He
    The Babraham Institute, Babraham Research Campus, Cambridge CB22 3AT, UK
    N Biotechnol 26:277-81. 2009
    ..The method has promise for library screening of pairwise protein interactions, down to the analytical level of individual domain or motif mapping...
  7. ncbi request reprint Arraying proteins by cell-free synthesis
    Mingyue He
    Technology Research Group, The Babraham Institute, Cambridge CB22 3AT, United Kingdom
    Biomol Eng 24:375-80. 2007
    ..This article reviews current cell-free protein array technologies and their proteomic applications...
  8. doi request reprint Eukaryotic ribosome display with in situ DNA recovery
    Mingyue He
    The Inositide Laboratory, The Babraham Institute, Cambridge, UK
    Methods Mol Biol 805:75-85. 2012
    ....
  9. pmc Effects of mutation at the D-JH junction on affinity, specificity, and idiotypy of anti-progesterone antibody DB3
    Mingyue He
    Technology Research Group, The Babraham Institute, Cambridge, United Kingdom
    Protein Sci 15:2141-8. 2006
    ....
  10. ncbi request reprint Advancing protein therapeutics: current challenges in protein therapeutics -- CHI's Second Annual Conference
    Mingyue He
    The Babraham Institute, Babraham Research Campus, Cambridge CB2 4AT, UK
    IDrugs 9:177-8. 2006
  11. pmc Single step generation of protein arrays from DNA by cell-free expression and in situ immobilisation (PISA method)
    M He
    Technology Research Group, The Babraham Institute, Babraham, Cambridge CB2 4AT, UK
    Nucleic Acids Res 29:E73-3. 2001
    ..Here we show that human single-chain antibody fragments (three domain, V(H)/K form) and an enzyme (luciferase) can be functionally arrayed by the PISA method...
  12. ncbi request reprint Ribosome display: next-generation display technologies for production of antibodies in vitro
    Mingyue He
    Protein Technologies Laboratory, Babraham Research Campus, Cambridge, CB2 4AT, UK
    Expert Rev Proteomics 2:421-30. 2005
    ..In the near future, cell-free protein synthesis may be developed as an alternative for large-scale generation of antibodies...
  13. ncbi request reprint DiscernArray technology: a cell-free method for the generation of protein arrays from PCR DNA
    Mingyue He
    Discerna Limited, Babraham Research Campus, Babraham, CB2 4AT, Cambridge, UK
    J Immunol Methods 274:265-70. 2003
    ..DiscernArray is particularly useful for arraying proteins and domains which cannot be functionally produced in heterologous expression systems or for which the cloned DNA is not available...
  14. ncbi request reprint Ribosome display of antibodies: expression, specificity and recovery in a eukaryotic system
    Mingyue He
    Protein Technologies Laboratory, The Babraham Institute, The Babraham Research Campus, Cambridge CB2 4AT, UK
    J Immunol Methods 297:73-82. 2005
    ..Our findings confirm the effectiveness of the eukaryotic ribosome display system and define conditions for efficient selection of single chain antibodies...
  15. ncbi request reprint Generation of protein in situ arrays by DiscernArray technology
    Mingyue He
    Discerna Limited, Cambridge, UK
    Methods Mol Biol 264:25-31. 2004
    ..Protein arrays of single-chain antibodies, ligand-binding domains, and enzymes have been successfully generated by this method...
  16. ncbi request reprint Cell-free protein synthesis for proteomics
    Alison M Jackson
    Discerna Ltd, Babraham Research Campus, Babraham, Cambridge CB2 4AT, UK
    Brief Funct Genomic Proteomic 2:308-19. 2004
    ..This paper reviews the most commonly used cell-free systems and their applications in proteomics...
  17. doi request reprint Protein microarrays: high-throughput tools for proteomics
    Oda Stoevesandt
    Babraham Bioscience Technologies Ltd, Babraham Research Campus, Cambridge, CB22 3AT, UK
    Expert Rev Proteomics 6:145-57. 2009
    ..This article reviews recent developments in the generation of protein microarrays and their applications in proteomics and diagnostics...
  18. doi request reprint Selection of recombinant antibodies by eukaryotic ribosome display
    Mingyue He
    Technology Research Group, The Babraham Institute, Cambridge, UK
    Methods Mol Biol 484:193-205. 2008
    ..We describe our eukaryotic system, in which rabbit reticulocyte extracts are used for cell free transcription/translation and cDNA is recovered by in situ RT-PCR performed on the selected complexes...
  19. ncbi request reprint Eukaryotic ribosome display with in situ DNA recovery
    Mingyue He
    Technology Research Group, The Babraham Institute, Cambridge CB22 4AT, UK
    Nat Methods 4:281-8. 2007
    ..We also introduce a recent, previously unpublished improvement to the procedure in which in situ reverse transcription is combined with sensitive single-primer PCR technology...
  20. pmc Enhanced cell-free protein expression by fusion with immunoglobulin Ckappa domain
    Elizabeth Palmer
    Technology Research Group, The Babraham Institute, Cambridge CB2 4AT, United Kingdom
    Protein Sci 15:2842-6. 2006
    ....
  21. doi request reprint In situ biosynthesis of peptide arrays
    Mingyue He
    The Babraham Institute, Cambridge, UK
    Methods Mol Biol 615:345-56. 2010
    ..Our methods allow making polypeptide arrays using amplified linear DNA fragments...
  22. doi request reprint Production of protein arrays by cell-free systems
    Mingyue He
    Technology Research Group, The Babraham Institute, Cambridge, UK
    Methods Mol Biol 484:207-15. 2008
    ..Advantages of PISA include avoiding bacterial expression and protein purification and making functional protein arrays available as required from genetic information...
  23. ncbi request reprint Production of human single-chain antibodies by ribosome display
    Mingyue He
    Discerna Ltd, Babraham Hall, Babraham, Cambridge, UK
    Methods Mol Biol 248:177-89. 2004
  24. doi request reprint Printing protein arrays from DNA arrays
    Mingyue He
    Technology Research Group, The Babraham Institute, Cambridge CB22 3AT, UK
    Nat Methods 5:175-7. 2008
    ..DAPA eliminates the need for separate protein expression, purification and spotting, and also overcomes the problem of long-term functional storage of surface-bound proteins...
  25. ncbi request reprint Ribosome display: cell-free protein display technology
    Mingyue He
    Discerna Limited, Babraham, Cambridge, UK
    Brief Funct Genomic Proteomic 1:204-12. 2002
    ..Both prokaryotic and eukaryotic ribosome display systems have been developed and each has its own distinctive features. In this paper, ribosome display systems and their application in selection and evolution of proteins are reviewed...
  26. ncbi request reprint Double-hexahistidine tag with high-affinity binding for protein immobilization, purification, and detection on ni-nitrilotriacetic acid surfaces
    Farid Khan
    Protein Technologies Laboratory, The Babraham Institute, Babraham Research Campus, Cambridge CB2 4AT, UK
    Anal Chem 78:3072-9. 2006
    ..The double-His6 peptide has the potential to be a universal tag for protein immobilization and detection on arrays and single-step purification of proteins from crude mixtures...
  27. ncbi request reprint The rapid discovery of engineered antibodies
    Mingrong Wang
    Centre for Protein Therapeutics, Sichuan Industrial Institute of Antibiotics, Sichuan 610051, China
    IDrugs 10:562-5. 2007
    ..The combination of ribosome display, selection and cell-free screening provides a rapid discovery tool for the generation, optimization and modification of antibody-combining sites...
  28. ncbi request reprint Selection of antigenic markers on a GFP-Ckappa fusion scaffold with high sensitivity by eukaryotic ribosome display
    Yong Min Yang
    The Institute of Genetics, San Diego, CA 92121 2233, USA
    Biochem Biophys Res Commun 359:251-7. 2007
    ..This platform is highly specific and sensitive for the antigen-antibody interaction and may permit selection and reshaping of high affinity antigenic variants of scaffold proteins...
  29. ncbi request reprint Improved affinity coupling for antibody microarrays: engineering of double-(His)6-tagged single framework recombinant antibody fragments
    Cornelia Steinhauer
    Department of Immunotechnology, Lund University, Lund, Sweden
    Proteomics 6:4227-34. 2006
    ..Taken together, the results demonstrate the generic potential of double-(His)(6)-tag recombinant antibodies for the facile fabrication of high-density antibody microarrays...