Research Topics
Genomes and Genes | L M FieldSummaryAffiliation: Institute for Animal Health Country: UK Publications
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Detail Information
Publications
Temporal synergism can enhance carbamate and neonicotinoid insecticidal activity against resistant crop pestsGeorgina Bingham
Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK
Pest Manag Sci 64:81-5. 2008..This concept is now applied to carbamates and neonicotinoids...
Identification of the main malaria vectors in the Anopheles gambiae species complex using a TaqMan real-time PCR assayChris Bass
Department of Biological Chemistry, Rothamsted Research, Harpenden, AL5 2JQ, UK
Malar J 6:155. 2007..Recently, a high-throughput assay based on TaqMan single nucleotide polymorphism genotyping that detects and discriminates An. gambiae s.s and An. arabiensis has been reported...
PCR-based detection of Plasmodium in Anopheles mosquitoes: a comparison of a new high-throughput assay with existing methodsChris Bass
Department of Biological Chemistry, Center for Sustainable Pest and Disease Management, Rothamsted Research, Harpenden, UK
Malar J 7:177. 2008..In this study a new high-throughput assay was developed and compared with three previously described PCR techniques...
Development of multiplex real-time PCR assays for identification of members of the Anopheles funestus species groupSamuel B Vezenegho
Vector Control Reference Unit, National Institute for Communicable Diseases of the NHLS, Private Bag X4, Sandringham, Johannesburg 2131, South Africa
Malar J 8:282. 2009..In this study, three new high-throughput 'closed-tube' assays were developed and compared with the previously described AS-PCR technique...
Detection of knockdown resistance (kdr) mutations in Anopheles gambiae: a comparison of two new high-throughput assays with existing methodsChris Bass
Department of Biological Chemistry, Rothamsted Research, Harpenden, UK
Malar J 6:111. 2007..However, there are few reports comparing the performance of these different assays. In this study, two new high-throughput assays were developed and compared with four established techniques...
Relationship between amount of esterase and gene copy number in insecticide-resistant Myzus persicae (Sulzer)L M Field
IACR Rothamsted, Harpenden, Herts AL5 2JQ, UK
Biochem J 339:737-42. 1999..For E4 genes, the loss of 5-methylcytosine is correlated with a loss of expression, greatly decreasing the amount of enzyme relative to the copy number...
Amplified esterase genes and their relationship with other insecticide resistance mechanisms in English field populations of the aphid, Myzus persicae (Sulzer)Linda M Field
Biological Chemistry Division, IACR Rothamsted, Harpenden, Hertfordshire AL5 2JQ, UK
Pest Manag Sci 58:889-94. 2002..The selection pressures and other factors leading to these changes in field populations of M persicae are discussed...
Methylation and expression of amplified esterase genes in the aphid Myzus persicae (Sulzer)L M Field
IACR Rothamsted, Harpenden, Herts AL5 2JQ, U K
Biochem J 349:863-8. 2000....
Identification of mutations conferring insecticide-insensitive AChE in the cotton-melon aphid, Aphis gossypii GloverM C Andrews
Biological Chemistry, Rothamsted Research, Harpenden, Herts, UK
Insect Mol Biol 13:555-61. 2004..A less specific insensitivity to a wide range of carbamates and organophosphates is associated with a second mutation, A302S (A201 in T. californica)...
Genome annotation and comparative analyses of the odorant-binding proteins and chemosensory proteins in the pea aphid Acyrthosiphon pisumJ J Zhou
Department of Biological Chemistry, Harpenden, Hertfordshire, UK
Insect Mol Biol 19:113-22. 2010..Our results support the 'birth-and-death' model as the major mechanism explaining aphid OBP sequence evolution, with the main force acting on the evolution being purifying selection...
DDT, pyrethrins, pyrethroids and insect sodium channelsT G E Davies
Biological Chemistry Division, Rothamsted Research, Hertfordshire, UK
IUBMB Life 59:151-62. 2007..Here we review some of the work (done at Rothamsted Research and elsewhere) that has led to the identification of specific residues on the sodium channel that may constitute the DDT and pyrethroid binding sites...
A comparative study of voltage-gated sodium channels in the Insecta: implications for pyrethroid resistance in Anopheline and other Neopteran speciesT G E Davies
Biological Chemistry Division, Rothamsted Research, Harpenden, Hertfordshire, UK
Insect Mol Biol 16:361-75. 2007....
Overexpression of a cytochrome P450 monooxygenase, CYP6ER1, is associated with resistance to imidacloprid in the brown planthopper, Nilaparvata lugensC Bass
Centre for Sustainable Pest and Disease Management, Rothamsted Research, Harpenden, UK
Insect Mol Biol 20:763-73. 2011..lugens strains was significantly correlated with the resistance phenotype. These results provide strong evidence for a role of CYP6ER1 in the resistance of N. lugens to imidacloprid...
The re-emergence of the bed bug as a nuisance pest: implications of resistance to the pyrethroid insecticidesT G E Davies
Department of Biological Chemistry, Rothamsted Research, Harpenden, UK
Med Vet Entomol 26:241-54. 2012..Here we review the literature on this topic, highlighting the mechanisms thought to be involved and the problems currently faced by pest control professionals in dealing with a developing pandemic...
OS-D-like genes and their expression in aphids (Hemiptera: Aphididae)S P Jacobs
Biological Chemistry Division, Rothamsted Research, Harpenden, Herts, UK
Insect Mol Biol 14:423-32. 2005..This supports the view that the proteins could be involved in chemoreception but our preliminary binding studies failed to detect binding to a range of compounds which are known to elicit an electrophysiological response by aphids...
Characterization of acetylcholinesterases, and their genes, from the hemipteran species Myzus persicae (Sulzer), Aphis gossypii (Glover), Bemisia tabaci (Gennadius) and Trialeurodes vaporariorum (Westwood)N Javed
Rothamsted Research, Harpenden, Herts, UK
Insect Mol Biol 12:613-20. 2003....
Identification of odorant-binding proteins of the yellow fever mosquito Aedes aegypti: genome annotation and comparative analysesJ J Zhou
Biological Chemistry Department, Rothamsted Research, Harpenden, UK
Insect Mol Biol 17:147-63. 2008..aegypti OBPs suggests that a rapid expansion of OBPs has occurred, probably by gene duplication. The analyses of OBP-containing regions for microsynteny indicate a very high synteny between Ae. aegypti and An. gambiae...
Cloning and characterisation of a prenyltransferase from the aphid Myzus persicae with potential involvement in alarm pheromone biosynthesisM J Lewis
Rothamsted Research, Harpenden, Herts, AL5 2JQ, UK
Insect Mol Biol 17:437-43. 2008..In addition, the enzyme can utilise geranyl pyrophosphate to produce farnesyl pyrophosphate showing that the synthesis of the latter involves the sequential condensation of isoprenyl pyrophosphate units...
Expression of chemosensory proteins in the tsetse fly Glossina morsitans morsitans is related to female host-seeking behaviourR Liu
Department of Biological Chemistry, Rothamsted Research, Harpenden, UK
Insect Mol Biol 21:41-8. 2012..Genome annotation and phylogenetic analyses comparing G. morsitans morsitans CSPs with those of other Diptera showed rapid evolution after speciation of mosquitoes...
DNA methylation in insectsL M Field
BCH Division, Rothamsted Research, Harpenden, Herts, UK
Insect Mol Biol 13:109-15. 2004....
Characterisation of target-site resistance to ACCase-inhibiting herbicides in the weed Alopecurus myosuroides (black-grass)Stephen R Moss
Division of Plant and Invertebrate Ecology, IACR Rothamsted, Harpenden, Hertfordshire AL5 2JQ, UK
Pest Manag Sci 59:190-201. 2003....
"Plus-C" odorant-binding protein genes in two Drosophila species and the malaria mosquito Anopheles gambiaeJing Jiang Zhou
Biological Chemistry Division, Rothamsted Research, Harpenden, Herts AL5 2JQ, UK
Gene 327:117-29. 2004..melanogaster. Related genes were found in all of the other species and the relationships of these with the D. melanogaster genes and their possible biological functions are discussed...
Development of a multiplex real-time PCR assay for identification of members of the Anopheles gambiae species complexChris Bass
Centre for Sustainable Pest and Disease Management, Biological Chemistry Department, Rothamsted Research, Harpenden, Hertfordshire, AL5 2JQ, UK
Acta Trop 107:50-3. 2008....
Molecular characterisation of nicotinic acetylcholine receptor subunits from the cat flea, Ctenocephalides felis (Siphonaptera: Pulicidae)Chris Bass
Department of Biological and Ecological Chemistry, Rothamsted Research, Harpenden, AL5 2JQ, UK
Insect Biochem Mol Biol 36:86-96. 2006....
Identification of the Rdl mutation in laboratory and field strains of the cat flea, Ctenocephalides felis (Siphonaptera: Pulicidae)Chris Bass
Biological Chemistry Division, Rothamsted Research, Harpenden, AL5 2JQ, United Kingdom
Pest Manag Sci 60:1157-62. 2004..The A302S mutation was present at a high frequency in these domestic pet populations...
Identification and expression of odorant-binding proteins of the malaria-carrying mosquitoes Anopheles gambiae and Anopheles arabiensisZheng-Xi Li
Biological Chemistry Division, Rothamsted Research, Harpenden, Hertfordshire, United Kingdom
Arch Insect Biochem Physiol 58:175-89. 2005..gambiae. The expression profiles of the genes in the two members of the Anopheles complex provides the first step towards further molecular analysis of the mosquito olfactory apparatus...
Temporal synergism by microencapsulation of piperonyl butoxide and alpha-cypermethrin overcomes insecticide resistance in crop pestsGeorgina Bingham
Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK
Pest Manag Sci 63:276-81. 2007..tabaci on cotton, eliminating the need for two treatments. The ratio between the active insecticide and the synergist was found to be crucial in reducing resistance factors...
Genome and EST analyses and expression of a gene family with putative functions in insect chemoreceptionJing Jiang Zhou
Biological Chemistry Division, Rothamsted Research, Harpenden, AL5 2JQ, UK
Chem Senses 31:453-65. 2006..mori compound eyes. The different frequencies of CSP transcripts were observed between solitary and gregarious EST libraries of Locusta migratoria...
Modelling insecticide-binding sites in the voltage-gated sodium channelBhupinder P S Khambay
Department of Crystallography, Birkbeck College, University of London, London WC1E 7HX, United Kingdom
Biochem J 396:255-63. 2006..Additional binding contacts on the II S4-S5 linker can explain the higher potency of pyrethroid insecticides compared with DDT...
Cloning and functional characterisation of a cis-muuroladiene synthase from black peppermint (Menthaxpiperita) and direct evidence for a chemotype unable to synthesise farneseneIan M Prosser
Rothamsted Research, Harpenden, West Common, Herts, AL5 2JQ, UK
Phytochemistry 67:1564-71. 2006..Analysis, by GC-MS, of the sesquiterpene profile of the plant used for the cloning, revealed that EbetaF was not present, confirming that this particular mint chemotype had lost EbetaF synthase activity due to the observed mutations...
Revisiting the odorant-binding protein LUSH of Drosophila melanogaster: evidence for odour recognition and discriminationJing Jiang Zhou
Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK
FEBS Lett 558:23-6. 2004....
Aphid alarm pheromone produced by transgenic plants affects aphid and parasitoid behaviorMichael H Beale
Rothamsted Research, Harpenden AL5 2JQ, United Kingdom
Proc Natl Acad Sci U S A 103:10509-13. 2006..Here, we report the transformation of a plant to produce an insect pheromone and demonstrate that the resulting emission affects behavioral responses at two trophic levels...
