David Barford

Summary

Affiliation: Institute of Cancer Research
Country: UK

Publications

  1. pmc Insights into degron recognition by APC/C coactivators from the structure of an Acm1-Cdh1 complex
    Jun He
    Division of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK
    Mol Cell 50:649-60. 2013
  2. pmc Structures of APC/C(Cdh1) with substrates identify Cdh1 and Apc10 as the D-box co-receptor
    Paula C A da Fonseca
    Section of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK
    Nature 470:274-8. 2011
  3. pmc Structural insights into anaphase-promoting complex function and mechanism
    David Barford
    Division of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK
    Philos Trans R Soc Lond B Biol Sci 366:3605-24. 2011
  4. ncbi request reprint The role of cysteine residues as redox-sensitive regulatory switches
    David Barford
    Section of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK
    Curr Opin Struct Biol 14:679-86. 2004
  5. doi request reprint Structure, function and mechanism of the anaphase promoting complex (APC/C)
    David Barford
    Section of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London, SW3 6JB, UK
    Q Rev Biophys 44:153-90. 2011
  6. pmc Doc1 mediates the activity of the anaphase-promoting complex by contributing to substrate recognition
    Lori A Passmore
    Section of Structural Biology and Cancer Research UK Centre for Cell and Molecular Biology, Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK
    EMBO J 22:786-96. 2003
  7. ncbi request reprint Structural analysis of the anaphase-promoting complex reveals multiple active sites and insights into polyubiquitylation
    Lori A Passmore
    Section of Structural Biology, The Institute of Cancer Research, Chester Beatty Laboratories, London, UK
    Mol Cell 20:855-66. 2005
  8. ncbi request reprint Purification and assay of the budding yeast anaphase-promoting complex
    Lori A Passmore
    Section of Structural Biology, Institute for Cancer Research, London SW3 6JB, UK
    Methods Enzymol 398:195-219. 2005
  9. pmc AKAP18 contains a phosphoesterase domain that binds AMP
    Matthew G Gold
    Section of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK
    J Mol Biol 375:1329-43. 2008
  10. doi request reprint Recombinant expression, reconstitution and structure of human anaphase-promoting complex (APC/C)
    Ziguo Zhang
    Division of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK
    Biochem J 449:365-71. 2013

Detail Information

Publications63

  1. pmc Insights into degron recognition by APC/C coactivators from the structure of an Acm1-Cdh1 complex
    Jun He
    Division of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK
    Mol Cell 50:649-60. 2013
    ..We provide a structural rationalization for D box and KEN box recognition by coactivators and demonstrate that many noncanonical APC/C degrons bind APC/C coactivators at the D box coreceptor...
  2. pmc Structures of APC/C(Cdh1) with substrates identify Cdh1 and Apc10 as the D-box co-receptor
    Paula C A da Fonseca
    Section of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK
    Nature 470:274-8. 2011
    ..Our results rationalize the contribution of both co-activator and core APC/C subunits to D-box recognition and provide a structural framework for understanding mechanisms of substrate recognition and catalysis by the APC/C...
  3. pmc Structural insights into anaphase-promoting complex function and mechanism
    David Barford
    Division of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK
    Philos Trans R Soc Lond B Biol Sci 366:3605-24. 2011
    ..Mechanisms of regulating and modulating substrate recognition are discussed in the context of controlling the binding of the co-activator to the APC/C, and the accessibility and conformation of the co-activator when bound to the APC/C...
  4. ncbi request reprint The role of cysteine residues as redox-sensitive regulatory switches
    David Barford
    Section of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK
    Curr Opin Struct Biol 14:679-86. 2004
    ....
  5. doi request reprint Structure, function and mechanism of the anaphase promoting complex (APC/C)
    David Barford
    Section of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London, SW3 6JB, UK
    Q Rev Biophys 44:153-90. 2011
    ....
  6. pmc Doc1 mediates the activity of the anaphase-promoting complex by contributing to substrate recognition
    Lori A Passmore
    Section of Structural Biology and Cancer Research UK Centre for Cell and Molecular Biology, Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK
    EMBO J 22:786-96. 2003
    ..These results imply that Doc1p/Apc10 may play a role to regulate the binding of specific substrates, similar to that of the coactivators...
  7. ncbi request reprint Structural analysis of the anaphase-promoting complex reveals multiple active sites and insights into polyubiquitylation
    Lori A Passmore
    Section of Structural Biology, The Institute of Cancer Research, Chester Beatty Laboratories, London, UK
    Mol Cell 20:855-66. 2005
    ..Our data suggest that multimerisation and/or the presence of multiple active sites facilitates the APC/C's ability to elongate polyubiquitin chains...
  8. ncbi request reprint Purification and assay of the budding yeast anaphase-promoting complex
    Lori A Passmore
    Section of Structural Biology, Institute for Cancer Research, London SW3 6JB, UK
    Methods Enzymol 398:195-219. 2005
    ..Activity assays toward several endogenous substrates, including Clb2 and Pds1, are described. In addition, methods for observation of APC-coactivator and APC-substrate complexes by native gel electrophoresis are described...
  9. pmc AKAP18 contains a phosphoesterase domain that binds AMP
    Matthew G Gold
    Section of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK
    J Mol Biol 375:1329-43. 2008
    ..It may act as a phosphoesterase, although we did not identify a substrate, or as an AMP sensor with the potential to couple intracellular AMP levels to PKA signalling events...
  10. doi request reprint Recombinant expression, reconstitution and structure of human anaphase-promoting complex (APC/C)
    Ziguo Zhang
    Division of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK
    Biochem J 449:365-71. 2013
    ..Additional density present in the human APC/C structure, proximal to Apc3/Cdc27 of the TPR lobe, is assigned to the TPR subunit Apc7, a subunit specific to vertebrate APC/C...
  11. doi request reprint Structural basis for the subunit assembly of the anaphase-promoting complex
    Anne Schreiber
    Section of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London, SW3 6JB, UK
    Nature 470:227-32. 2011
    ....
  12. ncbi request reprint Crystal structure of an activated Akt/protein kinase B ternary complex with GSK3-peptide and AMP-PNP
    Jing Yang
    Section of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London, SW3 6JB, UK
    Nat Struct Biol 9:940-4. 2002
    ..Analysis of the interactions between PKB and the GSK3beta-peptide explains how PKB selects for protein substrates distinct from those of PKA...
  13. ncbi request reprint The structure of Tap42/alpha4 reveals a tetratricopeptide repeat-like fold and provides insights into PP2A regulation
    Jing Yang
    Section of Structural Biology, Chester Beatty Laboratories, Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK
    Biochemistry 46:8807-15. 2007
    ..We propose a scaffolding function for Tap42/alpha4 whereby the interaction of PP2Ac at its N-terminus promotes the dephosphorylation of substrates recruited to the C-terminal region of the molecule...
  14. doi request reprint Structure of the mitotic checkpoint complex
    William C H Chao
    Division of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London, SW3 6JB, UK
    Nature 484:208-13. 2012
    ..This study shows how APC/C inhibition is coupled to degron recognition by co-activators...
  15. pmc The structure of the cell cycle protein Cdc14 reveals a proline-directed protein phosphatase
    Christopher H Gray
    Section of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK
    EMBO J 22:3524-35. 2003
    ..Our structural and kinetic data support a role for Cdc14 in the preferential dephosphorylation of proteins modified by proline-directed kinases...
  16. pmc Mechanism of farnesylated CAAX protein processing by the intramembrane protease Rce1
    Ioannis Manolaridis
    1 Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK 2
    Nature 504:301-5. 2013
    ..This study suggests that Rce1 is the founding member of a novel IMP family, the glutamate IMPs. ..
  17. ncbi request reprint Reversible oxidation of the membrane distal domain of receptor PTPalpha is mediated by a cyclic sulfenamide
    Jing Yang
    Section of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, United Kingdom
    Biochemistry 46:709-19. 2007
    ..Conformational changes associated with RPTPalpha D2 oxidation have implications for RPTPalpha quaternary structure and allosteric regulation of D1 phosphatase activity...
  18. ncbi request reprint Wild-type and mutant B-RAF activate C-RAF through distinct mechanisms involving heterodimerization
    Mathew J Garnett
    Signal Transduction Team, The Institute of Cancer Research, Cancer Research UK Centre of Cell and Molecular Biology, London
    Mol Cell 20:963-9. 2005
    ..Thus, we have identified a B-RAF-C-RAF-MEK-ERK cascade that signals not only in cancer but also in normal cells...
  19. ncbi request reprint Molecular basis of AKAP specificity for PKA regulatory subunits
    Matthew G Gold
    Section of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, United Kingdom
    Mol Cell 24:383-95. 2006
    ....
  20. pmc An alpha-helical extension of the ELMO1 pleckstrin homology domain mediates direct interaction to DOCK180 and is critical in Rac signaling
    David Komander
    Section of Structural Biology, Chester Beatty Laboratories, Institute of Cancer Research, London SW3 6JB, United Kingdom
    Mol Biol Cell 19:4837-51. 2008
    ..Significantly, although this does not affect DOCK180 GEF activity toward Rac in vivo, Rac signaling is impaired, implying additional roles for ELMO in mediating intracellular Rac signaling...
  21. pmc The four canonical tpr subunits of human APC/C form related homo-dimeric structures and stack in parallel to form a TPR suprahelix
    Ziguo Zhang
    Division of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK
    J Mol Biol 425:4236-48. 2013
    ..Remarkably, the uniform relative packing of neighboring TPR proteins generates a novel left-handed suprahelical TPR assembly. This finding has implications for understanding the assembly of other TPR-containing multimeric complexes. ..
  22. ncbi request reprint Lining the pockets of kinases and phosphatases
    Matthew G Gold
    Section of Structural Biology, Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK
    Curr Opin Struct Biol 16:693-701. 2006
    ..The crystal structure of the PP1 catalytic subunit in complex with the targeting subunit MYPT1 indicates that there is also scope for intimate phosphatase regulation by scaffold proteins...
  23. doi request reprint Activation of Rho GTPases by DOCK exchange factors is mediated by a nucleotide sensor
    Jing Yang
    Section of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK
    Science 325:1398-402. 2009
    ..These studies identify an unusual mechanism of GDP release and define the complete GEF catalytic cycle from GDP dissociation followed by GTP binding and discharge of the activated GTPase...
  24. pmc Enhancement of the seed-target recognition step in RNA silencing by a PIWI/MID domain protein
    James S Parker
    Institute of Cancer Research, Chester Beatty Laboratories, London, UK
    Mol Cell 33:204-14. 2009
    ..Thus, association of the guide strand with the PIWI/MID domain generates an enhanced affinity anchor site over the seed that can promote fidelity in target recognition and stabilize and guide the assembly of the active silencing complex...
  25. doi request reprint The structure of the CYLD USP domain explains its specificity for Lys63-linked polyubiquitin and reveals a B box module
    David Komander
    Section of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK
    Mol Cell 29:451-64. 2008
    ..Biochemical and functional characterization of the B box suggests a role as a protein-interaction module that contributes to determining the subcellular localization of CYLD...
  26. doi request reprint Molecular structure of the N-terminal domain of the APC/C subunit Cdc27 reveals a homo-dimeric tetratricopeptide repeat architecture
    Ziguo Zhang
    Section of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK
    J Mol Biol 397:1316-28. 2010
    ..These results establish the existence of functional APC/C genes in E. cuniculi, the evolutionarily conserved dimeric properties of Cdc27, and provide a framework for understanding the architecture of full-length Cdc27...
  27. pmc Mechanism of multi-site phosphorylation from a ROCK-I:RhoE complex structure
    David Komander
    Section of Structural Biology, The Institute of Cancer Research, Chester Beatty Laboratories, London, UK
    EMBO J 27:3175-85. 2008
    ..We propose that ROCK-I phosphorylation of RhoE forms part of a feedback loop to regulate RhoA signalling...
  28. doi request reprint A Raf-induced allosteric transition of KSR stimulates phosphorylation of MEK
    Damian F Brennan
    Section of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK
    Nature 472:366-9. 2011
    ..We propose that KSR interacts with a regulatory Raf molecule in cis to induce a conformational switch of MEK, facilitating MEK's phosphorylation by a separate catalytic Raf molecule in trans...
  29. pmc Molecular basis for TPR domain-mediated regulation of protein phosphatase 5
    Jing Yang
    Section of Structural Biology, The Institute of Cancer Research, Chester Beatty Laboratories, London, UK
    EMBO J 24:1-10. 2005
    ....
  30. ncbi request reprint Structure of the A20 OTU domain and mechanistic insights into deubiquitination
    David Komander
    Section of Structural Biology, The Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK
    Biochem J 409:77-85. 2008
    ..Biochemical analysis shows a preference of the isolated A20 OTU domain for Lys48-linked tetraubiquitin in vitro suggesting that additional specificity factors might be required for the physiological function of A20 in cells...
  31. pmc Structural insights into mRNA recognition from a PIWI domain-siRNA guide complex
    James S Parker
    Section of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK
    Nature 434:663-6. 2005
    ..This study provides insights into mechanisms of target mRNA recognition and cleavage by an Argonaute-siRNA guide complex...
  32. pmc The APC/C subunit Cdc16/Cut9 is a contiguous tetratricopeptide repeat superhelix with a homo-dimer interface similar to Cdc27
    Ziguo Zhang
    Section of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, London, UK
    EMBO J 29:3733-44. 2010
    ..This finding may provide a structural explanation for a mechanism to control the stoichiometry of proteins participating in multisubunit complexes...
  33. pmc Crystal structure of a PIWI protein suggests mechanisms for siRNA recognition and slicer activity
    James S Parker
    Section of Structural Biology, The Institute of Cancer Research, Chester Beatty Laboratories, London, UK
    EMBO J 23:4727-37. 2004
    ....
  34. pmc Coactivator functions in a stoichiometric complex with anaphase-promoting complex/cyclosome to mediate substrate recognition
    Lori A Passmore
    Section of Structural Biology, The Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK
    EMBO Rep 6:873-8. 2005
    ..A direct and stoichiometric function for the coactivators could explain how specific substrates are recognized by APC/C in a cell-cycle-specific manner, and how coactivator stimulates APC/C ubiquitylation activity...
  35. ncbi request reprint Molecular mechanism for the regulation of protein kinase B/Akt by hydrophobic motif phosphorylation
    Jing Yang
    Section of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK
    Mol Cell 9:1227-40. 2002
    ....
  36. pmc Building a pseudo-atomic model of the anaphase-promoting complex
    Kiran Kulkarni
    Division of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, England
    Acta Crystallogr D Biol Crystallogr 69:2236-43. 2013
    ....
  37. doi request reprint The structure of the 26S proteasome subunit Rpn2 reveals its PC repeat domain as a closed toroid of two concentric α-helical rings
    Jun He
    Division of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, United Kingdom
    Structure 20:513-21. 2012
    ..These findings have implications for understanding how 19S-RPs recognize, unfold, and deliver ubiquitylated substrates to the 20S core...
  38. ncbi request reprint Redox regulation of protein tyrosine phosphatase 1B involves a sulphenyl-amide intermediate
    Annette Salmeen
    Section of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK
    Nature 423:769-73. 2003
    ....
  39. ncbi request reprint Protein tyrosine phosphatases: X-ray crystallographic observation of cysteinyl-phosphate reaction intermediate
    David Barford
    Section of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, London SW3 6JB, United Kingdom
    Methods Enzymol 354:237-51. 2002
  40. ncbi request reprint Getting in the ring: proline-directed substrate specificity in the cell cycle proteins Cdc14 and CDK2-cyclinA3
    Christopher H Gray
    Section of Structural Biology, Chester Beatty Laboratories, Institute of Cancer Research, 237 Fulham Road, London SW3 6JB UK
    Cell Cycle 2:500-2. 2003
  41. ncbi request reprint Regulation of protein kinases in insulin, growth factor and Wnt signalling
    Laurence H Pearl
    Section of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK
    Curr Opin Struct Biol 12:761-7. 2002
    ..Structural studies of GSK3beta complexes are contributing to our understanding of the phosphorylation-independent mechanism that insulates the Wnt and insulin/growth factor pathways...
  42. doi request reprint Baculovirus expression: tackling the complexity challenge
    David Barford
    Division of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK
    Curr Opin Struct Biol 23:357-64. 2013
    ..Here we highlight recent major achievements in multiprotein complex structure research that were catalyzed by this versatile recombinant complex expression tool...
  43. doi request reprint Mechanism of isoprenylcysteine carboxyl methylation from the crystal structure of the integral membrane methyltransferase ICMT
    Jing Yang
    Division of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, London SW3 6JB, UK
    Mol Cell 44:997-1004. 2011
    ..This study explains how an integral membrane methyltransferase achieves recognition of both a hydrophilic cofactor and a lipophilic prenyl group attached to a polar protein substrate...
  44. pmc Multiple factors confer specific Cdc42 and Rac protein activation by dedicator of cytokinesis (DOCK) nucleotide exchange factors
    Kiran Kulkarni
    Section of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, United Kingdom
    J Biol Chem 286:25341-51. 2011
    ..DOCK proteins, therefore, differ from DH-PH GEFs that select their cognate GTPases through recognition of structural differences within the β2/β3 strands...
  45. ncbi request reprint Argonaute: A scaffold for the function of short regulatory RNAs
    James S Parker
    Section of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London, SW3 6JB, UK
    Trends Biochem Sci 31:622-30. 2006
    ....
  46. pmc Getting into position: the catalytic mechanisms of protein ubiquitylation
    Lori A Passmore
    Section of Structural Biology, The Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK
    Biochem J 379:513-25. 2004
    ..In particular, we discuss the complexity of molecular recognition required to impose selectivity on substrate selection and topology of poly-ubiquitin chains...
  47. ncbi request reprint Structure of TOR and its complex with KOG1
    Alessandra Adami
    Section of Structural Biology, Chester Beatty Laboratories, Institute of Cancer Research, 237 Fulham Road, London, UK
    Mol Cell 27:509-16. 2007
    ..A model is proposed for the molecular architecture of the TOR-KOG1 complex explaining its sensitivity to rapamycin...
  48. ncbi request reprint Mechanism of activation of the RAF-ERK signaling pathway by oncogenic mutations of B-RAF
    Paul T C Wan
    Section of Structural Biology, The Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK
    Cell 116:855-67. 2004
    ..The high activity mutants signal to ERK by directly phosphorylating MEK, whereas the impaired activity mutants stimulate MEK by activating endogenous C-RAF, possibly via an allosteric or transphosphorylation mechanism...
  49. ncbi request reprint Implications for the ubiquitination reaction of the anaphase-promoting complex from the crystal structure of the Doc1/Apc10 subunit
    Shannon W N Au
    Section of Structural Biology, Chester Beatty Laboratories, Institute of Cancer Research, 237 Fulham Road, London, SW3 6JB, UK
    J Mol Biol 316:955-68. 2002
    ....
  50. doi request reprint Eliminylation: a post-translational modification catalyzed by phosphothreonine lyases
    Damian F Brennan
    Section of Structural Biology, The Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London, SW3 6JB, UK
    Trends Biochem Sci 34:108-14. 2009
    ..We postulate that eliminylation might be a widespread regulatory modification, and we propose the use of phosphothreonine lyases as in vivo MAPK inhibitors both therapeutically and to investigate MAPK signalling regulation...
  51. ncbi request reprint Crystal structure of the PP2A phosphatase activator: implications for its PP2A-specific PPIase activity
    Nicolas Leulliot
    Institut de Biochimie et de Biophysique Moleculaire et Cellulaire, UMR8619, Bat 430, Universite de Paris Sud, 91405 Orsay Cedex, France
    Mol Cell 23:413-24. 2006
    ..Structure-guided mutational studies showed that in vivo PTPA activity is influenced by mutations on the surface of the peptide binding pocket, the same mutations that also influenced the in vitro activation of PP2Ai and PPIase activity...
  52. ncbi request reprint PTPN11 (Shp2) mutations in LEOPARD syndrome have dominant negative, not activating, effects
    Maria I Kontaridis
    Cancer Biology Program, Division of Hematology Oncology, Department of Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02115, USA
    J Biol Chem 281:6785-92. 2006
    ..Our results establish that the pathogenesis of LS and NS is distinct and suggest that these disorders should be distinguished by mutational analysis rather than clinical presentation...
  53. ncbi request reprint Conformational diversity in the TPR domain-mediated interaction of protein phosphatase 5 with Hsp90
    Matthew J Cliff
    Department of Biochemistry and Molecular Biology, University College London, Darwin Building, Gower Street, London WC1E 6BT, United Kingdom
    Structure 14:415-26. 2006
    ..We consider the possible implications of this dynamic structure for the mechanism of relief of autoinhibition in Ppp5 and for the mechanisms of TPR-mediated recognition of Hsp90 by other proteins...
  54. ncbi request reprint Molecular recognition via coupled folding and binding in a TPR domain
    Matthew J Cliff
    Department of Biochemistry and Molecular Biology, University College London, Darwin Building, Gower Street, London WC1E 6BT, UK
    J Mol Biol 346:717-32. 2005
    ..We hypothesise that a coupled folding-binding mechanism is common in this class of domains...
  55. ncbi request reprint Differential oxidation of protein-tyrosine phosphatases
    Arnoud Groen
    Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
    J Biol Chem 280:10298-304. 2005
    ..In conclusion, PTPs are differentially oxidized at physiological pH and H(2)O(2) concentrations, and the PTP loop arginine is an important determinant for susceptibility to oxidation...
  56. ncbi request reprint Crystal structure of a complex between protein tyrosine phosphatase 1B and the insulin receptor tyrosine kinase
    Shiqing Li
    Structural Biology Program, Skirball Institute of Biomolecular Medicine and Department of Pharmacology, New York University School of Medicine, New York, New York 10016, USA
    Structure 13:1643-51. 2005
    ..The crystal structure provides evidence for a noncatalytic mode of interaction between PTP1B and IRK, which could be important for the selective recruitment of PTP1B to the insulin receptor...
  57. ncbi request reprint Structural studies with inhibitors of the cell cycle regulatory kinase cyclin-dependent protein kinase 2
    Louise N Johnson
    Laboratory of Molecular Biophysics, Department of Biochemistry, University of Oxford, Rex Richards Building, South Parks Road, OX1 3QU, Oxford, UK
    Pharmacol Ther 93:113-24. 2002
    ..We describe the structure of phospho-CDK2 in complex with kinase-associated phosphatase, and discuss the substrate recognition promoted by interactions that are remote from the catalytic site...
  58. pmc A family with severe insulin resistance and diabetes due to a mutation in AKT2
    Stella George
    Department of Clinical Biochemistry, University of Cambridge, Addenbrooke s Hospital, Hills Road, Cambridge CB2 2QQ, UK
    Science 304:1325-8. 2004
    ..These findings demonstrate the central importance of AKT signaling to insulin sensitivity in humans...
  59. ncbi request reprint Identification of inhibitors of protein kinase B using fragment-based lead discovery
    Gordon Saxty
    J Med Chem 50:2293-6. 2007
    ..Iterative structure-based design was supported by protein-ligand structure determinations using a PKA-PKB "chimera" and a final protein-ligand structure of a lead compound in PKBbeta itself...
  60. ncbi request reprint Sorafenib functions to potently suppress RET tyrosine kinase activity by direct enzymatic inhibition and promoting RET lysosomal degradation independent of proteasomal targeting
    Ivan Plaza-Menacho
    Breakthrough Breast Cancer Research Centre, Institute of Cancer Research, SW3 6JB London, United Kingdom
    J Biol Chem 282:29230-40. 2007
    ..In addition, because inhibition of RET is not impaired by mutation of the Val(804) gatekeeper residue, MEN2 tumors may be less susceptible to acquired Sorafenib resistance...
  61. ncbi request reprint Crystal structure and functional analysis of the eukaryotic class II release factor eRF3 from S. pombe
    Chunguang Kong
    Laboratory of Macromolecular Structure, Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609, Japan
    Mol Cell 14:233-45. 2004
    ..The N-terminal extension, rich in acidic amino acids, blocks the proposed eRF1 binding site, potentially regulating eRF1 binding to eRF3 in a competitive manner...
  62. ncbi request reprint Functions and mechanisms of redox regulation of cysteine-based phosphatases
    Annette Salmeen
    Department of Molecular Pharmacology, Stanford University Medical School, Stanford, CA, USA
    Antioxid Redox Signal 7:560-77. 2005
    ..Formation of either type of covalent bond may be accompanied by dramatic structural rearrangements that can affect downstream signaling events and allow for multitiered enzyme regulation...
  63. ncbi request reprint A structural comparison of inhibitor binding to PKB, PKA and PKA-PKB chimera
    Thomas G Davies
    Astex Therapeutics Ltd, 436 Cambridge Science Park, Milton Road, Cambridge, CB4 0QA, UK
    J Mol Biol 367:882-94. 2007
    ..We provide a structural explanation for this difference, and highlight the ability of PKA-PKB to mimic the true PKB binding mode in this case...