S C West

Summary

Affiliation: Imperial Cancer Research Fund
Country: UK

Publications

  1. ncbi request reprint Processing of recombination intermediates by the RuvABC proteins
    S C West
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire, United Kingdom
    Annu Rev Genet 31:213-44. 1997
  2. pmc RuvA and RuvB proteins of Escherichia coli exhibit DNA helicase activity in vitro
    I R Tsaneva
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire, United Kingdom
    Proc Natl Acad Sci U S A 90:1315-9. 1993
  3. ncbi request reprint Cloning, overexpression, purification, and characterization of the Escherichia coli RuvC Holliday junction resolvase
    H J Dunderdale
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire, U K
    J Biol Chem 269:5187-94. 1994
  4. pmc Identification and purification of two distinct complexes containing the five RAD51 paralogs
    J Y Masson
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, UK
    Genes Dev 15:3296-307. 2001
  5. ncbi request reprint Reconstitution of the strand invasion step of double-strand break repair using human Rad51 Rad52 and RPA proteins
    M J McIlwraith
    Clare Hall Laboratories, Imperial Cancer Research Fund, South Mimms, Hertfordshire, EN6 3LD, UK
    J Mol Biol 304:151-64. 2000
  6. ncbi request reprint Late steps in genetic recombination: branch migration and Holliday junction resolution by RuvA, RuvB, and RuvC proteins
    S C West
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts, United Kingdom
    Cold Spring Harb Symp Quant Biol 58:525-31. 1993
  7. ncbi request reprint Role of BRCA2 in control of the RAD51 recombination and DNA repair protein
    A A Davies
    Imperial Cancer Research Fund, Clare Hall Laboratories, Hertfordshire EN6 3LD, South Mimms, United Kingdom
    Mol Cell 7:273-82. 2001
  8. pmc The meiosis-specific recombinase hDmc1 forms ring structures and interacts with hRad51
    J Y Masson
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, UK
    EMBO J 18:6552-60. 1999
  9. ncbi request reprint Branch migration and Holliday junction resolution catalyzed by activities from mammalian cells
    A Constantinou
    Imperial Cancer Research Fund, Clare Hall Laboratories, Blanche Lane, South Mimms, EN6 3LD, Hertfordshire, United Kingdom
    Cell 104:259-68. 2001
  10. ncbi request reprint Bypass of DNA heterologies during RuvAB-mediated three- and four-strand branch migration
    D E Adams
    Imperial Cancer Research Fund, South Mimms, Herts, UK
    J Mol Biol 263:582-96. 1996

Collaborators

Detail Information

Publications47

  1. ncbi request reprint Processing of recombination intermediates by the RuvABC proteins
    S C West
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire, United Kingdom
    Annu Rev Genet 31:213-44. 1997
    ..Genetic and biochemical studies indicate that branch migration and resolution are coupled by direct interactions between the three proteins, possibly by the formation of a RuvABC complex...
  2. pmc RuvA and RuvB proteins of Escherichia coli exhibit DNA helicase activity in vitro
    I R Tsaneva
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire, United Kingdom
    Proc Natl Acad Sci U S A 90:1315-9. 1993
    ..These results show that the ruvA and ruvB genes encode a DNA helicase that specifically recognizes Holliday junctions and promotes branch migration...
  3. ncbi request reprint Cloning, overexpression, purification, and characterization of the Escherichia coli RuvC Holliday junction resolvase
    H J Dunderdale
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire, U K
    J Biol Chem 269:5187-94. 1994
    ..0). No energy cofactors are needed. When RuvC was analyzed by gel filtration and polyacrylamide gel electrophoresis, monomeric and dimeric forms of the protein were observed...
  4. pmc Identification and purification of two distinct complexes containing the five RAD51 paralogs
    J Y Masson
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, UK
    Genes Dev 15:3296-307. 2001
    ..We suggest that the extreme sensitivity of paralog-defective cell lines to cross-linking agents is owing to defects in the processing of incised cross links and the consequential failure to initiate recombinational repair at these sites...
  5. ncbi request reprint Reconstitution of the strand invasion step of double-strand break repair using human Rad51 Rad52 and RPA proteins
    M J McIlwraith
    Clare Hall Laboratories, Imperial Cancer Research Fund, South Mimms, Hertfordshire, EN6 3LD, UK
    J Mol Biol 304:151-64. 2000
    ....
  6. ncbi request reprint Late steps in genetic recombination: branch migration and Holliday junction resolution by RuvA, RuvB, and RuvC proteins
    S C West
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts, United Kingdom
    Cold Spring Harb Symp Quant Biol 58:525-31. 1993
  7. ncbi request reprint Role of BRCA2 in control of the RAD51 recombination and DNA repair protein
    A A Davies
    Imperial Cancer Research Fund, Clare Hall Laboratories, Hertfordshire EN6 3LD, South Mimms, United Kingdom
    Mol Cell 7:273-82. 2001
    ..Thus, BRCA2 regulates both the intracellular localization and DNA binding ability of RAD51. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis...
  8. pmc The meiosis-specific recombinase hDmc1 forms ring structures and interacts with hRad51
    J Y Masson
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, UK
    EMBO J 18:6552-60. 1999
    ..Consistent with the requirement for two recombinases in meiotic recombination, hDmc1 interacts directly with hRad51...
  9. ncbi request reprint Branch migration and Holliday junction resolution catalyzed by activities from mammalian cells
    A Constantinou
    Imperial Cancer Research Fund, Clare Hall Laboratories, Blanche Lane, South Mimms, EN6 3LD, Hertfordshire, United Kingdom
    Cell 104:259-68. 2001
    ..coli RuvABC, highlighting the preservation of this essential pathway in recombination and DNA repair from prokaryotes to mammals...
  10. ncbi request reprint Bypass of DNA heterologies during RuvAB-mediated three- and four-strand branch migration
    D E Adams
    Imperial Cancer Research Fund, South Mimms, Herts, UK
    J Mol Biol 263:582-96. 1996
    ..Under certain conditions, the bypass of heterology is stimulated by the single-strand binding protein SSB...
  11. pmc The E.coli RuvAB proteins branch migrate Holliday junctions through heterologous DNA sequences in a reaction facilitated by SSB
    C A Parsons
    Genetic Recombination Laboratory, Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts, UK
    EMBO J 14:5736-44. 1995
    ..These results show that the RuvAB proteins, which are induced as part of the SOS response to DNA damage, allow genetic recombination and the recombinational repair of DNA to occur in the presence of extensive lengths of heterology...
  12. ncbi request reprint Substrate specificity of the Escherichia coli RuvC protein. Resolution of three- and four-stranded recombination intermediates
    F E Benson
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire, United Kingdom
    J Biol Chem 269:5195-201. 1994
    ..We interpret these differences in terms of the contiguity of the RecA nucleoprotein filament which may, under certain conditions, limit access to the Holliday junction resolvase...
  13. ncbi request reprint Formation and resolution of recombination intermediates by E. coli RecA and RuvC proteins
    H J Dunderdale
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts, UK
    Nature 354:506-10. 1991
    ..The newly identified RuvC protein resolves the intermediates by specific endonucleolytic cleavage to produce recombinant DNA molecules...
  14. pmc Resolution of Holliday junctions in vitro requires the Escherichia coli ruvC gene product
    B Connolly
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts, United Kingdom
    Proc Natl Acad Sci U S A 88:6063-7. 1991
    ..The UV sensitivity and deficiency in recombinational repair of DNA exhibited by ruv mutants lead us to suggest that RuvC resolves Holliday junctions in vivo...
  15. pmc Interaction of Escherichia coli RuvA and RuvB proteins with synthetic Holliday junctions
    C A Parsons
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts, United Kingdom
    Proc Natl Acad Sci U S A 89:5452-6. 1992
    ..We suggest that the RuvA and RuvB proteins play a specific role in the branch migration of Holliday junctions during postreplication repair of DNA damage in E. coli...
  16. pmc Visualization of recombination intermediates produced by RAD52-mediated single-strand annealing
    E Van Dyck
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, UK
    EMBO Rep 2:905-9. 2001
    ....
  17. ncbi request reprint The processing of recombination intermediates: mechanistic insights from studies of bacterial proteins
    S C West
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire, England
    Cell 76:9-15. 1994
  18. ncbi request reprint Human Rad51 protein promotes ATP-dependent homologous pairing and strand transfer reactions in vitro
    P Baumann
    Imperial Cancer Research Fund, South Mimms, Herts, United Kingdom
    Cell 87:757-66. 1996
    ..It is proposed that hRad51 mediates homology recognition and initiates strand exchange, but that extensive heteroduplex formation in higher organisms requires the actions of additional proteins...
  19. ncbi request reprint Synergistic actions of Rad51 and Rad52 in recombination and DNA repair
    F E Benson
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire, UK
    Nature 391:401-4. 1998
    ..Here we show that hRad52 stimulates homologous pairing by hRad51. The DNA-binding properties of hRad52 indicate that Rad52 is involved in an early stage of Rad51-mediated recombination...
  20. pmc RadA protein from Archaeoglobus fulgidus forms rings, nucleoprotein filaments and catalyses homologous recombination
    M J McIlwraith
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, UK
    Nucleic Acids Res 29:4509-17. 2001
    ..Finally, we have used electron microscopy to visualise RadA-mediated joint molecules, the intermediates of homologous recombination. Intriguingly, RadA shares properties of both the bacterial RecA and eukaryotic Rad51 recombinases...
  21. ncbi request reprint Resolution of Holliday junctions by RuvC resolvase: cleavage specificity and DNA distortion
    R J Bennett
    Imperial Cancer Research Fund, South Mimms, Hertfordshire, England
    Cell 74:1021-31. 1993
    ....
  22. pmc The RuvABC proteins and Holliday junction processing in Escherichia coli
    S C West
    Genetic Recombination Laboratory, Imperial Cancer Research Fund, South Mimms, United Kingdom
    J Bacteriol 178:1237-41. 1996
  23. pmc Precise binding of single-stranded DNA termini by human RAD52 protein
    C A Parsons
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, UK
    EMBO J 19:4175-81. 2000
    ..This protein-DNA arrangement may facilitate the DNA-DNA interactions necessary for RAD52-mediated annealing of complementary DNA strands...
  24. ncbi request reprint Effect of DNA topology on Holliday junction resolution by Escherichia coli RuvC and bacteriophage T7 endonuclease I
    D Zerbib
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts, EN6 3LD, U K
    J Mol Biol 270:663-73. 1997
    ..These results show that topological constraints affecting Holliday junction structure influence the orientation of resolution by cellular resolvases...
  25. pmc Complex formation by the human RAD51C and XRCC3 recombination repair proteins
    J Y Masson
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, United Kingdom
    Proc Natl Acad Sci U S A 98:8440-6. 2001
    ..Purified RAD51C--XRCC3 complex binds single-stranded, but not duplex DNA, to form protein--DNA networks that have been visualized by electron microscopy...
  26. pmc Activation of RuvC Holliday junction resolvase in vitro
    R Shah
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts, UK
    Nucleic Acids Res 22:2490-7. 1994
    ..These observations may indicate that other proteins are required for efficient RuvC-mediated resolution...
  27. ncbi request reprint XPG endonuclease makes the 3' incision in human DNA nucleotide excision repair
    A O'Donovan
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts, UK
    Nature 371:432-5. 1994
    ..These data show that XPG has a direct role in making one of the incisions required to excise a damaged oligonucleotide, by cleaving 3' to DNA damage during nucleotide excision repair...
  28. pmc Unusual stability of recombination intermediates made by Escherichia coli RecA protein
    B Muller
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts, London, UK
    EMBO J 11:2685-93. 1992
    ..We show that heteroduplex DNA may be formed in vitro by ATP-dependent strand exchange catalysed by RecA protein or by the RuvA and RuvB proteins of E. coli...
  29. ncbi request reprint ATP-dependent branch migration of Holliday junctions promoted by the RuvA and RuvB proteins of E. coli
    I R Tsaneva
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts, England
    Cell 69:1171-80. 1992
    ..The newly discovered biochemical properties of RuvA, RuvB, and RuvC are incorporated into a model for the postreplicational repair of DNA following UV irradiation...
  30. pmc Resolution of Holliday junctions in genetic recombination: RuvC protein nicks DNA at the point of strand exchange
    R J Bennett
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts, United Kingdom
    Proc Natl Acad Sci U S A 93:12217-22. 1996
    ..In these studies, junction targeting was achieved by incorporating uncharged methyl phosphonates into the DNA backbone, providing further evidence for the importance of charge-charge repulsions in determining DNA structure...
  31. ncbi request reprint The Rad51 and Dmc1 recombinases: a non-identical twin relationship
    J Y Masson
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, EN6 3LD, Herts, UK
    Trends Biochem Sci 26:131-6. 2001
    ..This review summarizes the key roles played by the two recombinases, Dmc1 and Rad51, in the faithful repair of DNA breaks...
  32. ncbi request reprint Purification and properties of the RuvA and RuvB proteins of Escherichia coli
    I R Tsaneva
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts, UK
    Mol Gen Genet 235:1-10. 1992
    ..Addition of purified RuvA and RuvB to a RecA-mediated recombination reaction was found to stimulate the rate of strand exchange leading to the rapid formation of heteroduplex DNA...
  33. pmc DNA end-joining catalyzed by human cell-free extracts
    P Baumann
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts EN6 3LD, United Kingdom
    Proc Natl Acad Sci U S A 95:14066-70. 1998
    ....
  34. ncbi request reprint Formation of RuvABC-Holliday junction complexes in vitro
    A A Davies
    Imperial Cancer Research Fund, Clare Hall Laboratories, Hertfordshire, UK
    Curr Biol 8:725-7. 1998
    ..Complex formation was not facilitated by duplex DNA. The identification of a RuvABC-junction complex provides direct evidence that the RuvABC proteins interact at the Holliday junction...
  35. ncbi request reprint Biological roles of the Escherichia coli RuvA, RuvB and RuvC proteins revealed
    S C West
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire, UK
    Mol Microbiol 6:2755-9. 1992
    ..The third protein, RuvC, which acts independently of RuvA and RuvB, resolves recombination intermediates by specific endonucleolytic cleavage of the Holliday junction...
  36. ncbi request reprint Genetic recombination in E. coli: RuvC protein cleaves Holliday junctions at resolution hotspots in vitro
    R Shah
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire, England
    Cell 79:853-64. 1994
    ..We propose that efficient RuvC resolution requires the translocation of Holliday junctions to specific cleavage sites, thus providing a biochemical basis for the similar genetic defects observed in ruvA, ruvB, and ruvC mutants...
  37. ncbi request reprint Binding of inositol phosphate to DNA-PK and stimulation of double-strand break repair
    L A Hanakahi
    Imperial Cancer Research Fund, South Mimms, Hertfordshire, United Kingdom
    Cell 102:721-9. 2000
    ..The involvement of inositol phosphate in DNA-PK-dependent NHEJ is of particular interest since the catalytic domain of DNA-PKcs is similar to that found in the phosphatidylinositol 3 (PI 3)-kinase family...
  38. ncbi request reprint Heteroduplex formation by human Rad51 protein: effects of DNA end-structure, hRP-A and hRad52
    P Baumann
    Clare Hall Laboratories, Imperial Cancer Research Fund, South Mimms, Hertfordshire, EN6 3LD, UK
    J Mol Biol 291:363-74. 1999
    ..Joint molecule formation by hRad51 was stimulated or inhibited by hRad52, dependent upon the reaction conditions. The inhibitory effect could be overcome by the presence of hRP-A or excess heterologous DNA...
  39. pmc Purification and characterization of the human Rad51 protein, an analogue of E. coli RecA
    F E Benson
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts, UK
    EMBO J 13:5764-71. 1994
    ..In contrast to yeast Rad51 protein, human Rad51 forms filaments with single-stranded DNA in the presence of ATP/ATP gamma S. These resemble the inactive form of the RecA filament which is observed in the absence of a nucleotide cofactor...
  40. ncbi request reprint BRCA2: a universal recombinase regulator
    T Thorslund
    London Research Institute, Cancer Research UK, Clare Hall Laboratories, South Mimms, Herts, UK
    Oncogene 26:7720-30. 2007
    ..The interactions of BRCA2 with RAD51 and DMC1 lead us to suggest that the BRCA2 tumour suppressor is a universal regulator of recombinase actions...
  41. ncbi request reprint Role of the Rad1 and Rad10 proteins in nucleotide excision repair and recombination
    A A Davies
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire, United Kingdom
    J Biol Chem 270:24638-41. 1995
    ..The role that Rad1-Rad10 plays in recombination is likely to resemble its activity in NER by acting upon partially unpaired DNA intermediates such as those formed by recombination mechanisms involving single-strand DNA annealing...
  42. ncbi request reprint Exchanging partners: recombination in E. coli
    A K Eggleston
    Clare Hall Laboratories, Herts, UK
    Trends Genet 12:20-6. 1996
    ..This finding holds promise that the development of in vitro systems for recombination by eukaryotic proteins lies in the not-too-distant future...
  43. pmc Domain structure and dynamics in the helical filaments formed by RecA and Rad51 on DNA
    X Yu
    Department of Biochemistry and Molecular Genetics, University of Virginia Health Sciences Center, Box 800733, Charlottesville, VA 22908, USA
    Proc Natl Acad Sci U S A 98:8419-24. 2001
    ..The N-terminal domain of Rad51 and the C-terminal domain of RecA may have arisen from convergent evolution to play similar roles in the filaments...
  44. pmc The Bloom's syndrome gene product promotes branch migration of holliday junctions
    J K Karow
    Imperial Cancer Research Fund Laboratories, Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, OX3 9DS, United Kingdom
    Proc Natl Acad Sci U S A 97:6504-8. 2000
    ..We present a model in which BLM disrupts potentially recombinogenic molecules that arise at sites of stalled replication forks. Our results have implications for the role of BLM as an anti-recombinase in the suppression of tumorigenesis...
  45. pmc Distribution of the Rad51 recombinase in human and mouse spermatocytes
    A L Barlow
    LSF Research Unit, Regional Genetics Services, Heartlands Hospital, Yardley Green Road, Birmingham B9 5PX, UK
    EMBO J 16:5207-15. 1997
    ..These interactions are spatially and temporally associated with synapsis during meiotic prophase I...
  46. pmc Nonhomologous end joining and V(D)J recombination require an additional factor
    Y Dai
    Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA
    Proc Natl Acad Sci U S A 100:2462-7. 2003
    ..These results provide strong evidence that additional activities are crucial for NHEJ and V(D)J recombination in mammals...
  47. ncbi request reprint The human Rad52 protein exists as a heptameric ring
    A Z Stasiak
    Laboratory of Ultrastructural Analysis, University of Lausanne, Lausanne, CH 1015, Switzerland
    Curr Biol 10:337-40. 2000
    ..Thus, the structures formed by the hexameric helicases may be a more general property of other proteins involved in DNA metabolism, including those, such as Rad52, that do not bind and hydrolyze ATP...