J Wain

Summary

Affiliation: Imperial College
Country: UK

Publications

  1. ncbi request reprint The emergence of multidrug resistance to antimicrobial agents for the treatment of typhoid fever
    John Wain
    Centre for Molecular Microbiology and Infection, Level 3, Flowers Building, Imperial College, South Kensington, London SW7 2AZ, UK
    Trans R Soc Trop Med Hyg 98:423-30. 2004
  2. pmc Molecular analysis of incHI1 antimicrobial resistance plasmids from Salmonella serovar Typhi strains associated with typhoid fever
    John Wain
    Wellcome Trust Clinical Research Unit, Department of Biological Sciences, Imperial College, London
    Antimicrob Agents Chemother 47:2732-9. 2003
  3. pmc Quantitation of bacteria in bone marrow from patients with typhoid fever: relationship between counts and clinical features
    J Wain
    Wellcome Trust Clinical Research Unit, Centre for Tropical Diseases, Ho Chi Minh City, Vietnam
    J Clin Microbiol 39:1571-6. 2001
  4. pmc Value of a single-tube widal test in diagnosis of typhoid fever in Vietnam
    C M Parry
    Wellcome Trust Clinical Research Unit, Cho Quan Hospital, Ho Chi Minh City, Vietnam
    J Clin Microbiol 37:2882-6. 1999
  5. pmc Nasal carriage in Vietnamese children of Streptococcus pneumoniae resistant to multiple antimicrobial agents
    C M Parry
    Wellcome Trust Clinical Research Unit, Cho Quan Hospital, District 5, Ho Chi Minh City, Vietnam
    Antimicrob Agents Chemother 44:484-8. 2000
  6. ncbi request reprint Melioidosis in Southern Vietnam: clinical surveillance and environmental sampling
    C M Parry
    Wellcome Trust Clinical Research Unit, Centre for Tropical Diseases, Ho Chi Minh City, Vietnam
    Clin Infect Dis 29:1323-6. 1999
  7. pmc Serology of typhoid fever in an area of endemicity and its relevance to diagnosis
    D House
    Department of Biochemistry, Imperial College of Science, Technology and Medicine, Exhibition Road, South Kensington, London SW7 2AZ, United Kingdom
    J Clin Microbiol 39:1002-7. 2001
  8. pmc Acquisition of virulence-associated factors by the enteric pathogens Escherichia coli and Salmonella enterica
    J Wain
    Centre for Molecular Microbiology and Infection, Imperial College of Science, Technology and Medicine, South Kensington, London W7 2AZ, UK
    Philos Trans R Soc Lond B Biol Sci 356:1027-34. 2001
  9. ncbi request reprint The Escherichia coli gene pool
    G Dougan
    Centre for Molecular Microbiology and Infection, Department of Biochemistry, Imperial College of Science, Technology and Medicine, Exhibition Road, SW7 2AZ, London, UK
    Curr Opin Microbiol 4:90-4. 2001
  10. ncbi request reprint Genes of the class II and class III major histocompatibility complex are associated with typhoid fever in Vietnam
    S J Dunstan
    Department of Biochemistry, Imperial College of Science, Technology and Medicine, London, SW7 2AZ, United Kingdom
    J Infect Dis 183:261-268. 2001

Collaborators

Detail Information

Publications47

  1. ncbi request reprint The emergence of multidrug resistance to antimicrobial agents for the treatment of typhoid fever
    John Wain
    Centre for Molecular Microbiology and Infection, Level 3, Flowers Building, Imperial College, South Kensington, London SW7 2AZ, UK
    Trans R Soc Trop Med Hyg 98:423-30. 2004
    ..These regions appear to be hot spots for DNA acquisition in IncHI1 plasmids...
  2. pmc Molecular analysis of incHI1 antimicrobial resistance plasmids from Salmonella serovar Typhi strains associated with typhoid fever
    John Wain
    Wellcome Trust Clinical Research Unit, Department of Biological Sciences, Imperial College, London
    Antimicrob Agents Chemother 47:2732-9. 2003
    ..In 1996 a single RFLP type, RFLP7, was found to carry the dfrA7 and sul-1 genes, which were not present on R27 or pHCM1. This plasmid type appears to have a selective advantage over other plasmids with the same resistance phenotype...
  3. pmc Quantitation of bacteria in bone marrow from patients with typhoid fever: relationship between counts and clinical features
    J Wain
    Wellcome Trust Clinical Research Unit, Centre for Tropical Diseases, Ho Chi Minh City, Vietnam
    J Clin Microbiol 39:1571-6. 2001
    ..01) and positively with fever clearance time after treatment (R = 0.4, P < 0.001). The bacterial load in bone marrow therefore may reflect the clinical course of the infection, and high levels may suppress neutrophil proliferation...
  4. pmc Value of a single-tube widal test in diagnosis of typhoid fever in Vietnam
    C M Parry
    Wellcome Trust Clinical Research Unit, Cho Quan Hospital, Ho Chi Minh City, Vietnam
    J Clin Microbiol 37:2882-6. 1999
    ..However, 14% of the positive results would be false-positive, and 10% of the negative results would be false-negative. The Widal test can be helpful in the laboratory diagnosis of typhoid fever in Vietnam if interpreted with care...
  5. pmc Nasal carriage in Vietnamese children of Streptococcus pneumoniae resistant to multiple antimicrobial agents
    C M Parry
    Wellcome Trust Clinical Research Unit, Cho Quan Hospital, District 5, Ho Chi Minh City, Vietnam
    Antimicrob Agents Chemother 44:484-8. 2000
    ..This study suggests that resistance to penicillin and other antimicrobial agents is common in carriage isolates of S. pneumoniae from children in Vietnam...
  6. ncbi request reprint Melioidosis in Southern Vietnam: clinical surveillance and environmental sampling
    C M Parry
    Wellcome Trust Clinical Research Unit, Centre for Tropical Diseases, Ho Chi Minh City, Vietnam
    Clin Infect Dis 29:1323-6. 1999
    ..The low incidence of melioidosis in the provinces around Ho Chi Minh City may be explained by the restricted distribution of ara-negative B. pseudomallei in the soil in this area...
  7. pmc Serology of typhoid fever in an area of endemicity and its relevance to diagnosis
    D House
    Department of Biochemistry, Imperial College of Science, Technology and Medicine, Exhibition Road, South Kensington, London SW7 2AZ, United Kingdom
    J Clin Microbiol 39:1002-7. 2001
    ..007). These tests could be of use for the diagnosis of typhoid fever in patients who have clinical typhoid fever but are culture negative or in regions where bacterial culturing facilities are not available...
  8. pmc Acquisition of virulence-associated factors by the enteric pathogens Escherichia coli and Salmonella enterica
    J Wain
    Centre for Molecular Microbiology and Infection, Imperial College of Science, Technology and Medicine, South Kensington, London W7 2AZ, UK
    Philos Trans R Soc Lond B Biol Sci 356:1027-34. 2001
    ..We show how acquisition of DNA at specific sites on the chromosome has contributed to increased genetic variation and virulence of these two genera of the Enterobacteriaceae...
  9. ncbi request reprint The Escherichia coli gene pool
    G Dougan
    Centre for Molecular Microbiology and Infection, Department of Biochemistry, Imperial College of Science, Technology and Medicine, Exhibition Road, SW7 2AZ, London, UK
    Curr Opin Microbiol 4:90-4. 2001
    ..coli to adapt to new environments. These additional genes are often clustered, have related functions (for example, virulence-associated genes in pathogenicity islands) and may be integrated at specific sites on the E. coli chromosome...
  10. ncbi request reprint Genes of the class II and class III major histocompatibility complex are associated with typhoid fever in Vietnam
    S J Dunstan
    Department of Biochemistry, Imperial College of Science, Technology and Medicine, London, SW7 2AZ, United Kingdom
    J Infect Dis 183:261-268. 2001
    ..DRB1*04) or predisposed individuals to typhoid fever (TNFA*2 [-308].DRB1*0301) were determined. This report identifies a genetic association in humans between typhoid fever and MHC class II and III genes...
  11. pmc Typhoid fever and genetic polymorphisms at the natural resistance-associated macrophage protein 1
    S J Dunstan
    Department of Biochemistry, Imperial College of Science, Technology and Medicine, London SW7 2AZ, United Kingdom
    J Infect Dis 183:1156-60. 2001
    ..No allelic association was identified between the NRAMP1 alleles and typhoid fever susceptibility. In addition, neither homozygotes nor heterozygotes for any NRAMP1 variants were at increased risk of typhoid fever...
  12. pmc Use of paired serum samples for serodiagnosis of typhoid fever
    Deborah House
    Centre for Molecular Microbiology and Infection, Department of Biological Sciences, Imperial College London, South Kensington, UK
    J Clin Microbiol 43:4889-90. 2005
    ....
  13. ncbi request reprint Cytokine release by lipopolysaccharide-stimulated whole blood from patients with typhoid fever
    Deborah House
    Centre for Molecular Microbiology and Infection, Imperial College of Science, Technology, and Medicine, London, United Kingdom
    J Infect Dis 186:240-5. 2002
    ..In acute typhoid fever, the ability of peripheral blood leukocytes to release proinflammatory cytokines in response to an inflammatory stimulus is depressed, and this may contribute to delayed recovery following antibiotic treatment...
  14. ncbi request reprint Unlocking the genome of the human typhoid bacillus
    John Wain
    Centre for Molecular Microbiology and Infection, Department of Biological Sciences, Imperial College of Science, Technology, and Medicine, London SW7 2AZ, UK
    Lancet Infect Dis 2:163-70. 2002
    ..We have new insights into the mechanisms underpinning the human host specificity of S typhi, and have exploitable new routes to improved diagnostics and a better understanding of the epidemiology of the disease...
  15. ncbi request reprint A clinical, microbiological, and pathological study of intestinal perforation associated with typhoid fever
    Quoc Chanh Nguyen
    Dong Thap Provincial Hospital, Dong Thap, Ho Chi Minh City, Vietnam
    Clin Infect Dis 39:61-7. 2004
    ..Immunohistochemical results showed that the predominant infiltrating cell types at the site of perforation were CD68+ leukocytes (macrophages) or CD3+ leukocytes (T lymphocytes)...
  16. pmc Randomized controlled comparison of ofloxacin, azithromycin, and an ofloxacin-azithromycin combination for treatment of multidrug-resistant and nalidixic acid-resistant typhoid fever
    Christopher M Parry
    Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam
    Antimicrob Agents Chemother 51:819-25. 2007
    ..Both antibiotics were well tolerated. Uncomplicated typhoid fever due to isolates of MDR S. enterica serovar Typhi with reduced susceptibility to fluoroquinolones (Na(r)) can be successfully treated with a 7-day course of azithromycin...
  17. pmc Antimicrobial drug resistance of Salmonella enterica serovar typhi in asia and molecular mechanism of reduced susceptibility to the fluoroquinolones
    Tran Thuy Chau
    Oxford University Clinical Research Unit, Hospital for Tropical Diseases, 190 Ben Ham Tu, Ho Chi Minh City, Vietnam
    Antimicrob Agents Chemother 51:4315-23. 2007
    ..The 8-methoxy fluoroquinolone gatifloxacin showed rapid killing of serovar Typhi harboring both the single- and double-amino-acid substitutions...
  18. ncbi request reprint Risk factors for typhoid fever in the Mekong delta, southern Viet Nam: a case-control study
    C Luxemburger
    Wellcome Trust Clinical Research Unit, Centre for Tropical Diseases, Ho Chi Minh City, Viet Nam
    Trans R Soc Trop Med Hyg 95:19-23. 2001
    ..These findings suggest that strategies directed towards the persons in contact with a patient might reduce the incidence of secondary cases of typhoid fever...
  19. ncbi request reprint Characterisation and distribution of a cryptic Salmonella typhi plasmid pHCM2
    Claire Kidgell
    Centre for Molecular Microbiology and Infection, Department of Biological Sciences, Imperial College of Science, Technology and Medicine, London SW7 2AY, UK
    Plasmid 47:159-71. 2002
    ..typhi isolated before, but not after, 1994. Similar pHCM2-related sequences were also detected in S. typhi isolated from other regions of South East Asia and Pakistan but not elsewhere in the world...
  20. pmc Vi antigen expression in Salmonella enterica serovar Typhi clinical isolates from Pakistan
    John Wain
    Investigative Sciences, Centre for Molecular Microbiology and Infection, Imperial College, London, United Kingdom
    J Clin Microbiol 43:1158-65. 2005
    ....
  21. ncbi request reprint Cases of typhoid fever imported into England, Scotland and Wales (2000-2003)
    Fiona J Cooke
    Health Protection Agency, Centre for Infections, 61 Colindale Avenue, London, UK
    Trans R Soc Trop Med Hyg 101:398-404. 2007
    ..This study helps to describe the epidemiology of antimicrobial drug resistance in typhoid fever...
  22. ncbi request reprint Comparative trial of short-course ofloxacin for uncomplicated typhoid fever in Vietnamese children
    Ha Vinh
    Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam
    Ann Trop Paediatr 25:17-22. 2005
    ..typhi. No adverse events were attributable to the ofloxacin. These results extend previous observations on the efficacy of short courses of ofloxacin for children with uncomplicated multi-drug-resistant typhoid fever...
  23. ncbi request reprint Typhoid fever: pathogenesis and disease
    D House
    Centre for Molecular Microbiology and Infection, Imperial College of Science Technology and Medicine, London, UK
    Curr Opin Infect Dis 14:573-8. 2001
    ..In addition, we attempt to put current salmonella research into the context of typhoid fever...
  24. ncbi request reprint Salmonella typhi, the causative agent of typhoid fever, is approximately 50,000 years old
    Claire Kidgell
    Centre for Molecular Microbiology and Infection, Imperial College of Science, Technology and Medicine, The Flowers Building, Exhibition Road, South Kensington, London SW7 2AY, UK
    Infect Genet Evol 2:39-45. 2002
    ..typhi existed 15,000-150,000 years ago, during the human hunter-gatherer phase and prior to the development of agriculture and the domestication of animals...
  25. ncbi request reprint Host susceptibility and clinical outcomes in toll-like receptor 5-deficient patients with typhoid fever in Vietnam
    Sarah J Dunstan
    Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam
    J Infect Dis 191:1068-71. 2005
    ..TLR5 may not play an important role in TLR-stimulated innate immune responses to human infection with Salmonella enterica serovar Typhi. Initiation of these responses may rely on other TLRs that recognize different bacterial ligands...
  26. pmc Composition, acquisition, and distribution of the Vi exopolysaccharide-encoding Salmonella enterica pathogenicity island SPI-7
    Derek Pickard
    Centre for Molecular Microbiology and Infection, Department of Biological Sciences, Imperial College of Science, Technology and Medicine, Armstrong Road, London SW7 2AZ, UK
    J Bacteriol 185:5055-65. 2003
    ..citri. This analysis suggested that SPI-7 may be a mobile element, such as a conjugative transposon or an integrated plasmid remnant...
  27. pmc Emergence in Vietnam of Streptococcus pneumoniae resistant to multiple antimicrobial agents as a result of dissemination of the multiresistant Spain(23F)-1 clone
    Christopher M Parry
    Wellcome Trust Clinical Research Unit, Ho Chi Minh City, Vietnam
    Antimicrob Agents Chemother 46:3512-7. 2002
    ..Simple clinical predictors can be used to guide empirical antibiotic therapy of meningitis. Pneumococcal vaccination may help to control this problem...
  28. ncbi request reprint Serodiagnosis of Salmonella enterica serovar Typhi and S. enterica serovars Paratyphi A, B and C human infections
    Henrik Chart
    Laboratory of Enteric Pathogens, Department of Gastrointestinal Infections, Centre for Infections, Health Protection Agency, 61 Colindale Avenue, London NW9 5EQ, UK
    J Med Microbiol 56:1161-6. 2007
    ..The Salmonella serodiagnosis protocols described here are the new standard operating procedures used by the Health Protection Agency's National Salmonella Reference Centre based in the Laboratory of Enteric Pathogens, Colindale, UK...
  29. pmc Prophage sequences defining hot spots of genome variation in Salmonella enterica serovar Typhimurium can be used to discriminate between field isolates
    Fiona J Cooke
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
    J Clin Microbiol 45:2590-8. 2007
    ..These multiplex PCR assays, based on prophage-like elements and Salmonella genomic island 1, provide a simple method for identifying new variants of S. enterica serovar Typhimurium in the field...
  30. pmc Multidrug-resistant Salmonella enterica serovar paratyphi A harbors IncHI1 plasmids similar to those found in serovar typhi
    Kathryn E Holt
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    J Bacteriol 189:4257-64. 2007
    ..Our data show that these serovar Typhi and Paratyphi A IncHI1 plasmids share highly conserved core DNA and have acquired similar mobile elements encoding antibiotic resistance genes in past decades...
  31. doi request reprint Emergence of highly fluoroquinolone-resistant Salmonella enterica serovar Typhi in a community-based fever surveillance from Kolkata, India
    Shanta Dutta
    Int J Antimicrob Agents 31:387-9. 2008
  32. ncbi request reprint Evaluation of new-generation serologic tests for the diagnosis of typhoid fever: data from a community-based surveillance in Calcutta, India
    Shanta Dutta
    National Institute of Cholera and Enteric Diseases, P 33 CIT Road, Scheme XM, Beliaghata, P O Box 177, Calcutta 700010, India
    Diagn Microbiol Infect Dis 56:359-65. 2006
    ..There is a need for more efficient rapid diagnostic test for typhoid fever especially during the acute stage of the disease. Until then, culture remains the method of choice...
  33. pmc Fluoroquinolone resistance in Salmonella Typhi
    Fiona J Cooke
    BMJ 333:353-4. 2006
  34. ncbi request reprint The acquisition of full fluoroquinolone resistance in Salmonella Typhi by accumulation of point mutations in the topoisomerase targets
    Arthur K Turner
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus Hinxton, Cambridge CB10 1SA, UK
    J Antimicrob Chemother 58:733-40. 2006
    ..To determine the contribution to fluoroquinolone resistance of point mutations in the gyrA and parC genes of Salmonella Typhi...
  35. pmc Molecular analysis of fluoroquinolone-resistant Salmonella Paratyphi A isolate, India
    Satheesh Nair
    The Wellcome Trust Sanger Institute, Cambridgeshire, United Kingdom
    Emerg Infect Dis 12:489-91. 2006
    ....
  36. pmc Detection of Vi-negative Salmonella enterica serovar typhi in the peripheral blood of patients with typhoid fever in the Faisalabad region of Pakistan
    Stephen Baker
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, UK
    J Clin Microbiol 43:4418-25. 2005
    ..The method described here can be used to monitor the incidence of Vi-negative serovar Typhi in regions where the Vi vaccine is used...
  37. pmc High-throughput sequencing provides insights into genome variation and evolution in Salmonella Typhi
    Kathryn E Holt
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Nat Genet 40:987-93. 2008
    ..The observed patterns of genetic isolation and drift are consistent with the proposed key role of asymptomatic carriers of Typhi as the main reservoir of this pathogen, highlighting the need for identification and treatment of carriers...
  38. pmc A study of typhoid fever in five Asian countries: disease burden and implications for controls
    R Leon Ochiai
    International Vaccine Institute, Seoul, Republic of Korea
    Bull World Health Organ 86:260-8. 2008
    ..To inform policy-makers about introduction of preventive interventions against typhoid, including vaccination...
  39. ncbi request reprint The role of prophage-like elements in the diversity of Salmonella enterica serovars
    Nicholas Thomson
    The Pathogen Sequencing Unit, The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
    J Mol Biol 339:279-300. 2004
    ..Lysogenic bacteriophages therefore play a crucial role in the generation of genetic diversity within S.enterica...
  40. pmc Molecular characterization of the Salmonella enterica serovar Typhi Vi-typing bacteriophage E1
    Derek Pickard
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
    J Bacteriol 190:2580-7. 2008
    ..The genome of the Vi phage E1 is significantly related to other bacteriophages belonging to the same serovar Typhi phage-typing set, and we demonstrate a role for phage DNA modification in determining host specificity...
  41. pmc Multilocus sequence typing analysis of Shigella flexneri isolates collected in Asian countries
    Seon Young Choi
    1International Vaccine Institute, San 4 8 Bongcheon 7 Dong, Kwanak gu, Seoul 151 818, Republic of Korea
    J Med Microbiol 56:1460-6. 2007
    ..S. flexneri serotypes 1-5, X and Y were clustered together in a group containing many allelic variants while serotype 6 formed a distinct group, as previously established...
  42. ncbi request reprint Double blind comparison of ibuprofen and paracetamol for adjunctive treatment of uncomplicated typhoid fever
    Ha Vinh
    Hospital for Tropical Diseases and University of Oxford Wellcome Trust Clinical Research Unit, Chi Minh City, Vietnam
    Pediatr Infect Dis J 23:226-30. 2004
    ..Antipyretics reduce the prolonged, high fever characteristic of typhoid fever. The benefits of nonsteroidal drugs in this role have not been quantified. There have been concerns about the safety of antipyretics in typhoid...
  43. pmc Enteroaggregative Escherichia coli related to uropathogenic clonal group A
    Faith Wallace-Gadsden
    Department of Biology, Haverford College, Haverford, Pennsylvania 19041, USA
    Emerg Infect Dis 13:757-60. 2007
    ..coli clonal group A belongs. This finding suggests a recent common ancestor for these distinct groups of pathogenic E. coli...
  44. pmc Salmonella paratyphi A rates, Asia
    R Leon Ochiai
    International Vaccine Institute, Kwanak PO Box 14, Seoul, South Korea 151 600
    Emerg Infect Dis 11:1764-6. 2005
    ..Paratyphi A. We compared their incidences by using standardized methods from population-based studies in China, Indonesia, India, and Pakistan...
  45. ncbi request reprint Deep-seated resistance in relapsed paratyphoid fever
    Graham S Cooke
    Department of Infectious Diseases, Hammersmith Hospital, London, United Kingdom
    Clin Infect Dis 42:e92-4. 2006
    ..18fluorodeoxyglucose positron emission tomography scanning identified deep-seated infection including unsuspected aortitis and highlights the utility of novel imaging techniques to improve our understanding and treatment of this disease...
  46. ncbi request reprint Isolation of Salmonella enterica subspecies enterica serovar Paratyphi B dT+, or Salmonella Java, from Indonesia and alteration of the d-tartrate fermentation phenotype by disrupting the ORF STM 3356
    Kyung Ho Han
    International Vaccine Institute, San 4 8 Bongcheon 7 Dong, Kwanak gu, Seoul, 151 818, Republic of Korea
    J Med Microbiol 55:1661-5. 2006
    ..The results show that the dT+ phenotype requires a functional product encoded by STM 3356, and support the use of the PCR-based discrimination method for S. Paratyphi B and S. Java as the standard differentiation method...
  47. ncbi request reprint An H-NS-like stealth protein aids horizontal DNA transmission in bacteria
    Marie Doyle
    Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College Dublin, Dublin 2, Ireland
    Science 315:251-2. 2007
    ..This stealth strategy seems to be used widely to aid horizontal DNA transmission and has important implications for bacterial evolution...