Affiliation: Imperial College
- Imaging the environment of green fluorescent proteinKlaus Suhling
Department of Biological Sciences, Sir Alexander Fleming Building, Imperial College of Science, Technology and Medicine, London SW7 1AZ, United Kingdom
Biophys J 83:3589-95. 2002..This novel approach paves the way for imaging the biophysical environment of specific GFP-tagged proteins in live cells...
- Time-resolved fluorescence anisotropy imaging applied to live cellsKlaus Suhling
Departments of Physics, Biological Sciences and Chemistry, Imperial College London, London SW7 2BW, UK
Opt Lett 29:584-6. 2004..We demonstrate imaging of solvent viscosity variations through the rotational correlation time of fluorescein in a multiwell plate and apply this technique to probe the microviscosity in live cells...
- Time-domain fluorescence lifetime imaging applied to biological tissueDan Elson
Photonics Group, Physics Department, Imperial College London, London, UK
Photochem Photobiol Sci 3:795-801. 2004..This article aims to review our recent progress in developing time-domain FLIM technology for microscopy and endoscopy and applying it to biological tissue...
- Fluorescence probe techniques to monitor protein adsorption-induced conformation changes on biodegradable polymersJohan Benesch
3B s Research Group Biomaterials, Biodegradables and Biomimetics, Department of Polymer Engineering, University of Minho, 4710 057 Braga, Portugal
J Colloid Interface Sci 312:193-200. 2007....
- Molecular diffusion within sol-gel derived matrices viewed via fluorescence recovery after photobleachingGraham Hungerford
Departamento de Fisica, Universidade do Minho, 4710 057, Braga, Portugal
Photochem Photobiol Sci 6:825-8. 2007....
- Imaging intracellular viscosity of a single cell during photoinduced cell deathMarina K Kuimova
Chemistry Department, Imperial College London, Exhibition Road, London SW7 2AZ, UK
Nat Chem 1:69-73. 2009..Using these tools, we provide insight into the dynamics of diffusion in cells, which is pertinent to drug delivery, cell signalling and intracellular mass transport...
- Molecular rotor measures viscosity of live cells via fluorescence lifetime imagingMarina K Kuimova
Chemistry Department, Imperial College London, Exhibition Road, SW7 2AZ, UK
J Am Chem Soc 130:6672-3. 2008..The high viscosity of intracellular compartments is likely to play an important role in vital intracellular processes, including the rate of diffusion of reactive oxygen species, causing programmed cell destruction...
- Photophysical properties and intracellular imaging of water-soluble porphyrin dimers for two-photon excited photodynamic therapyMarina K Kuimova
Chemistry Department, Imperial College London, Exhibition Road, London SW72AZ, UK
Org Biomol Chem 7:889-96. 2009..Analysis of the photophysical parameters and intracellular imaging data indicates that these dimers are promising candidates for one-photon and two-photon excited PDT...
- Fluorescence characterisation of multiply-loaded anti-HER2 single chain Fv-photosensitizer conjugates suitable for photodynamic therapyMarina K Kuimova
Chemistry Department, Imperial College London, Exhibition Road, UK
Photochem Photobiol Sci 6:933-9. 2007..The present study highlights the usefulness of photosensitizer immunoconjugates with scFvs for targeted PDT...
- Imaging proteins in vivo using fluorescence lifetime microscopyFrederic Festy
Randall Division of Cell and Molecular Biophysics, King s College London, London, UK SE1 1UL
Mol Biosyst 3:381-91. 2007..We review the principles and recent advances in the application of the technique, instrumentation and molecular probe development...
- Diffusion in a sol-gel-derived medium with a view toward biosensor applicationsGraham Hungerford
Departamento de Fisica, Universidade do Minho, 4710 057 Braga, Portugal
J Phys Chem B 111:3558-62. 2007..Diffusion coefficients of between ca. 0.5 x 10(-7) and 4 x 10(-7) cm2 s(-1) were obtained from preliminary FRAP measurements of fluorescein and correlated to differences in the catalytic activity of HRP incorporated in the monolith...
- Time-resolved fluorescence microscopyKlaus Suhling
Department of Physics, King s College London, Strand, London, UK
Photochem Photobiol Sci 4:13-22. 2005..In addition, FLIM can also probe the local environment of fluorophores, reporting, for example, on the local pH, refractive index, ion or oxygen concentration without the need for ratiometric measurements...
- Effect of refractive index on the fluorescence lifetime of green fluorescent proteinCarolyn Tregidgo
King s College London, Department of Physics, Strand, London, WC2R 2LS, United Kingdom
J Biomed Opt 13:031218. 2008..Our findings show that the GFP fluorescence lifetime is shortened in TIRF microscopy in comparison to confocal microscopy...
- Imaging immune surveillance by T cells and NK cellsFiona E McCann
Department of Biological Sciences, Sir Alexander Fleming Building, Imperial College of Science, Technology and Medicine, London, UK
Immunol Rev 189:179-92. 2002..Imaging parameters other than fluorescence intensity, such as the lifetime of the fluorophore's excited state, could be used to report on protein environments...