Research Topics
| Klaus SuhlingSummaryAffiliation: Imperial College Country: UK Publications
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Detail Information
Publications
Imaging the environment of green fluorescent proteinKlaus Suhling
Department of Biological Sciences, Sir Alexander Fleming Building, Imperial College of Science, Technology and Medicine, London SW7 1AZ, United Kingdom
Biophys J 83:3589-95. 2002..This novel approach paves the way for imaging the biophysical environment of specific GFP-tagged proteins in live cells...
Time-resolved fluorescence anisotropy imaging applied to live cellsKlaus Suhling
Departments of Physics, Biological Sciences and Chemistry, Imperial College London, London SW7 2BW, UK
Opt Lett 29:584-6. 2004..We demonstrate imaging of solvent viscosity variations through the rotational correlation time of fluorescein in a multiwell plate and apply this technique to probe the microviscosity in live cells...- Time-domain fluorescence lifetime imaging applied to biological tissueDan Elson
Photonics Group, Physics Department, Imperial College London, London, UK
Photochem Photobiol Sci 3:795-801. 2004..This article aims to review our recent progress in developing time-domain FLIM technology for microscopy and endoscopy and applying it to biological tissue... - Fluorescence probe techniques to monitor protein adsorption-induced conformation changes on biodegradable polymersJohan Benesch
3B s Research Group Biomaterials, Biodegradables and Biomimetics, Department of Polymer Engineering, University of Minho, 4710 057 Braga, Portugal
J Colloid Interface Sci 312:193-200. 2007.... - Molecular diffusion within sol-gel derived matrices viewed via fluorescence recovery after photobleachingGraham Hungerford
Departamento de Fisica, Universidade do Minho, 4710 057, Braga, Portugal
Photochem Photobiol Sci 6:825-8. 2007.... 
Imaging intracellular viscosity of a single cell during photoinduced cell deathMarina K Kuimova
Chemistry Department, Imperial College London, Exhibition Road, London SW7 2AZ, UK
Nat Chem 1:69-73. 2009..Using these tools, we provide insight into the dynamics of diffusion in cells, which is pertinent to drug delivery, cell signalling and intracellular mass transport...- Molecular rotor measures viscosity of live cells via fluorescence lifetime imagingMarina K Kuimova
Chemistry Department, Imperial College London, Exhibition Road, SW7 2AZ, UK
J Am Chem Soc 130:6672-3. 2008..The high viscosity of intracellular compartments is likely to play an important role in vital intracellular processes, including the rate of diffusion of reactive oxygen species, causing programmed cell destruction... - Photophysical properties and intracellular imaging of water-soluble porphyrin dimers for two-photon excited photodynamic therapyMarina K Kuimova
Chemistry Department, Imperial College London, Exhibition Road, London SW72AZ, UK
Org Biomol Chem 7:889-96. 2009..Analysis of the photophysical parameters and intracellular imaging data indicates that these dimers are promising candidates for one-photon and two-photon excited PDT... - Fluorescence characterisation of multiply-loaded anti-HER2 single chain Fv-photosensitizer conjugates suitable for photodynamic therapyMarina K Kuimova
Chemistry Department, Imperial College London, Exhibition Road, UK
Photochem Photobiol Sci 6:933-9. 2007..The present study highlights the usefulness of photosensitizer immunoconjugates with scFvs for targeted PDT... - Imaging proteins in vivo using fluorescence lifetime microscopyFrederic Festy
Randall Division of Cell and Molecular Biophysics, King s College London, London, UK SE1 1UL
Mol Biosyst 3:381-91. 2007..We review the principles and recent advances in the application of the technique, instrumentation and molecular probe development... - Diffusion in a sol-gel-derived medium with a view toward biosensor applicationsGraham Hungerford
Departamento de Fisica, Universidade do Minho, 4710 057 Braga, Portugal
J Phys Chem B 111:3558-62. 2007..Diffusion coefficients of between ca. 0.5 x 10(-7) and 4 x 10(-7) cm2 s(-1) were obtained from preliminary FRAP measurements of fluorescein and correlated to differences in the catalytic activity of HRP incorporated in the monolith... - Time-resolved fluorescence microscopyKlaus Suhling
Department of Physics, King s College London, Strand, London, UK
Photochem Photobiol Sci 4:13-22. 2005..In addition, FLIM can also probe the local environment of fluorophores, reporting, for example, on the local pH, refractive index, ion or oxygen concentration without the need for ratiometric measurements... - Effect of refractive index on the fluorescence lifetime of green fluorescent proteinCarolyn Tregidgo
King s College London, Department of Physics, Strand, London, WC2R 2LS, United Kingdom
J Biomed Opt 13:031218. 2008..Our findings show that the GFP fluorescence lifetime is shortened in TIRF microscopy in comparison to confocal microscopy... - Imaging immune surveillance by T cells and NK cellsFiona E McCann
Department of Biological Sciences, Sir Alexander Fleming Building, Imperial College of Science, Technology and Medicine, London, UK
Immunol Rev 189:179-92. 2002..Imaging parameters other than fluorescence intensity, such as the lifetime of the fluorophore's excited state, could be used to report on protein environments...