R E Sinden

Summary

Affiliation: Imperial College
Country: UK

Publications

  1. ncbi request reprint A biologist's perspective on malaria vaccine development
    Robert E Sinden
    Department of Life Sciences, Imperial College London, UK
    Hum Vaccin 6:3-11. 2010
  2. pmc Proteomic analysis of Plasmodium in the mosquito: progress and pitfalls
    M N Wass
    The Centre for Bioinformatics, Department of Life Sciences, Imperial College, London SW7 2AZ
    Parasitology 139:1131-45. 2012
  3. doi request reprint The design and interpretation of laboratory assays measuring mosquito transmission of Plasmodium
    Robert E Sinden
    The Malaria Centre, Imperial College London, South Kensington, London SW7 2AZ, UK
    Trends Parasitol 28:457-65. 2012
  4. pmc Reality check for malaria proteomics
    Robert E Sinden
    The Malaria Centre, Department of Life Sciences, Imperial College London, SW7 2AZ, UK
    Genome Biol 10:211. 2009
  5. pmc The biology of sexual development of Plasmodium: the design and implementation of transmission-blocking strategies
    Robert E Sinden
    The Jenner Institute, University of Oxford, Old Road Campus Research Building, Roosevelt Drive, Oxford OX3 7DQ, UK
    Malar J 11:70. 2012
  6. pmc Population biology of malaria within the mosquito: density-dependent processes and potential implications for transmission-blocking interventions
    Thomas S Churcher
    Department of Infectious Disease Epidemiology, School of Public Health, Faculty of Medicine, Imperial College London, UK
    Malar J 9:311. 2010
  7. pmc A semi-automated method for counting fluorescent malaria oocysts increases the throughput of transmission blocking studies
    Michael J Delves
    Division of Cell and Molecular Biology, Imperial College London, London, UK
    Malar J 9:35. 2010
  8. pmc Anopheles mortality is both age- and Plasmodium-density dependent: implications for malaria transmission
    Emma J Dawes
    Department of Infectious Disease Epidemiology, Faculty of Medicine, Imperial College London, London, UK
    Malar J 8:228. 2009
  9. pmc PCR-based detection of Plasmodium in Anopheles mosquitoes: a comparison of a new high-throughput assay with existing methods
    Chris Bass
    Department of Biological Chemistry, Center for Sustainable Pest and Disease Management, Rothamsted Research, Harpenden, UK
    Malar J 7:177. 2008
  10. pmc The proteome of Toxoplasma gondii: integration with the genome provides novel insights into gene expression and annotation
    Dong Xia
    Department of Pre clinical Veterinary Science, Faculty of Veterinary Science, University of Liverpool, Liverpool L69 7ZJ, UK
    Genome Biol 9:R116. 2008

Detail Information

Publications77

  1. ncbi request reprint A biologist's perspective on malaria vaccine development
    Robert E Sinden
    Department of Life Sciences, Imperial College London, UK
    Hum Vaccin 6:3-11. 2010
    ..The biological rational for approaches to limit parasite transmission are highlighted and their place in future efforts to improve the lives of the 40% of the world's population at risk of the disease is discussed...
  2. pmc Proteomic analysis of Plasmodium in the mosquito: progress and pitfalls
    M N Wass
    The Centre for Bioinformatics, Department of Life Sciences, Imperial College, London SW7 2AZ
    Parasitology 139:1131-45. 2012
    ..Some of the in silico predictions made have now been supported by ongoing protein tagging and genetic knockout studies. We hope this discussion may assist future studies...
  3. doi request reprint The design and interpretation of laboratory assays measuring mosquito transmission of Plasmodium
    Robert E Sinden
    The Malaria Centre, Imperial College London, South Kensington, London SW7 2AZ, UK
    Trends Parasitol 28:457-65. 2012
    ..However, the diverse readouts of these assays are not directly comparable. Here we attempt to identify the utility of each assay and provide rational frameworks by which to compare the impacts recorded by the diverse methodologies...
  4. pmc Reality check for malaria proteomics
    Robert E Sinden
    The Malaria Centre, Department of Life Sciences, Imperial College London, SW7 2AZ, UK
    Genome Biol 10:211. 2009
    ..New studies highlight the wide diversity of post-translational protein modifications in the intra-erythrocytic stages of the malaria parasite, raising new avenues for inquiry...
  5. pmc The biology of sexual development of Plasmodium: the design and implementation of transmission-blocking strategies
    Robert E Sinden
    The Jenner Institute, University of Oxford, Old Road Campus Research Building, Roosevelt Drive, Oxford OX3 7DQ, UK
    Malar J 11:70. 2012
    ....
  6. pmc Population biology of malaria within the mosquito: density-dependent processes and potential implications for transmission-blocking interventions
    Thomas S Churcher
    Department of Infectious Disease Epidemiology, School of Public Health, Faculty of Medicine, Imperial College London, UK
    Malar J 9:311. 2010
    ..These processes regulating the development of the malaria parasite within the mosquito may influence the success of transmission-blocking interventions (TBIs) currently under development...
  7. pmc A semi-automated method for counting fluorescent malaria oocysts increases the throughput of transmission blocking studies
    Michael J Delves
    Division of Cell and Molecular Biology, Imperial College London, London, UK
    Malar J 9:35. 2010
    ..is important for many of assays investigating transmission. However, current assays are very time-consuming, manually demanding and patently subject to observer-observer variation...
  8. pmc Anopheles mortality is both age- and Plasmodium-density dependent: implications for malaria transmission
    Emma J Dawes
    Department of Infectious Disease Epidemiology, Faculty of Medicine, Imperial College London, London, UK
    Malar J 8:228. 2009
    ..Previous studies illustrate conflicting evidence as to the importance of Plasmodium-induced vector mortality, but very few studies to date have considered the effect of infection density on mosquito survival...
  9. pmc PCR-based detection of Plasmodium in Anopheles mosquitoes: a comparison of a new high-throughput assay with existing methods
    Chris Bass
    Department of Biological Chemistry, Center for Sustainable Pest and Disease Management, Rothamsted Research, Harpenden, UK
    Malar J 7:177. 2008
    ..In this study a new high-throughput assay was developed and compared with three previously described PCR techniques...
  10. pmc The proteome of Toxoplasma gondii: integration with the genome provides novel insights into gene expression and annotation
    Dong Xia
    Department of Pre clinical Veterinary Science, Faculty of Veterinary Science, University of Liverpool, Liverpool L69 7ZJ, UK
    Genome Biol 9:R116. 2008
    ....
  11. ncbi request reprint A proteomic analysis of malaria biology: integration of old literature and new technologies
    R E Sinden
    Department of Biological Sciences, Sir Alexander Fleming Building, Imperial College London, Imperial College Road, London SW7 2AZ, UK
    Int J Parasitol 34:1441-50. 2004
    ..When viewed together, the old and new data can give rapid and penetrating insights into what some might now term the 'Systems-Biology' of Plasmodium...
  12. pmc Progression of Plasmodium berghei through Anopheles stephensi is density-dependent
    Robert E Sinden
    Division of Cell and Molecular Biology, Faculty of Life Sciences, Imperial College London, London, United Kingdom
    PLoS Pathog 3:e195. 2007
    ....
  13. pmc Malaria, mosquitoes and the legacy of Ronald Ross
    Robert E Sinden
    Division of Cell and Molecular Biology, Faculty of Natural Sciences, Imperial College, London, England
    Bull World Health Organ 85:894-6. 2007
  14. doi request reprint The flagellum in malarial parasites
    R E Sinden
    The Malaria Centre, The Department of Life Sciences, Imperial College London, London SW7 2AZ, United Kingdom
    Curr Opin Microbiol 13:491-500. 2010
    ....
  15. pmc Transgenic expression of a mosquito-stage malarial protein, Pbs21, in blood stages of transformed Plasmodium berghei and induction of an immune response upon infection
    G Margos
    Department of Biology, Imperial College of Science, Technology and Medicine, London, United Kingdom
    Infect Immun 66:3884-91. 1998
    ..The ability to express mosquito midgut-stage antigens in blood-stage parasites will facilitate further investigations of molecular and immunological properties of these proteins...
  16. ncbi request reprint Determination of mosquito bloodmeal pH in situ by ion-selective microelectrode measurement: implications for the regulation of malarial gametogenesis
    O Billker
    Department of Biology, Imperial College of Science, Technology and Medicine, London, UK
    Parasitology 120:547-51. 2000
    ....
  17. ncbi request reprint The dynamics of interactions between Plasmodium and the mosquito: a study of the infectivity of Plasmodium berghei and Plasmodium gallinaceum, and their transmission by Anopheles stephensi, Anopheles gambiae and Aedes aegypti
    Y Alavi
    Department of Biological Sciences, Imperial College London, London, SW7 2AZ, UK
    Int J Parasitol 33:933-43. 2003
    ..Understanding these at the molecular level may contribute to the development of rational strategies to reduce the vector competence of malarial vectors...
  18. ncbi request reprint Structure and expression of a post-transcriptionally regulated malaria gene encoding a surface protein from the sexual stages of Plasmodium berghei
    M G Paton
    Department of Biology, Imperial College of Science, Technology and Medicine, London, UK
    Mol Biochem Parasitol 59:263-75. 1993
    ..The Pbs21 gene and the P. berghei parasite system provide an excellent vehicle for the study of stage-specific transcriptional and post-transcriptional control in malaria...
  19. ncbi request reprint The SCID mouse as a laboratory model for development of the exoerythrocytic stages of human and rodent malaria
    G A Butcher
    Department of Biology, Imperial College of Science Technology and Medicine, London, United Kingdom
    Exp Parasitol 77:257-60. 1993
  20. ncbi request reprint Malaria parasites and the anopheles mosquito
    G Dimopoulos
    Department of Biological Sciences, Imperial College of Science, Technology and Medicine, London, UK
    Chem Immunol 80:27-49. 2002
  21. ncbi request reprint Mosquito--malaria interactions: a reappraisal of the concepts of susceptibility and refractoriness
    R E Sinden
    Department of Biological Sciences, Imperial College London, London SW7 2AZ, UK
    Insect Biochem Mol Biol 34:625-9. 2004
    ....
  22. ncbi request reprint The use of transgenic Plasmodium berghei expressing the Plasmodium vivax antigen P25 to determine the transmission-blocking activity of sera from malaria vaccine trials
    Souraya Ramjanee
    Division of Cell and Molecular Biology, Sir Alexander Fleming Building, Imperial College London, Imperial College Road, London SW7 2AZ, UK
    Vaccine 25:886-94. 2007
    ..To this end the cloned lines have been deposited with, and are freely available from, MR4...
  23. ncbi request reprint Identification of xanthurenic acid as the putative inducer of malaria development in the mosquito
    O Billker
    Department of Biology, Imperial College, London, UK
    Nature 392:289-92. 1998
    ..These data could form the basis of the rational development of new methods of interrupting the transmission of malaria using drugs or new refractory mosquito genotypes to block parasite gametogenesis...
  24. ncbi request reprint Both mosquito-derived xanthurenic acid and a host blood-derived factor regulate gametogenesis of Plasmodium in the midgut of the mosquito
    M Arai
    Department of Biology, Imperial College of Science, Technology and Medicine, London SW7 2AZ, UK
    Mol Biochem Parasitol 116:17-24. 2001
    ..Studies are underway to determine whether vertebrate-derived GAF is XA. These data may form the basis of further studies of the development of new methods of interrupting malarial transmission...
  25. pmc Female inheritance of malarial lap genes is essential for mosquito transmission
    J Dale Raine
    Division of Cell and Molecular Biology, Faculty of Natural Sciences, Imperial College London, London, United Kingdom
    PLoS Pathog 3:e30. 2007
    ..This phenotype is not seen in the other dividing forms of the mutant parasite lines in the liver and blood stages...
  26. pmc Transmission-blocking interventions eliminate malaria from laboratory populations
    A M Blagborough
    Department of Life Sciences, Imperial College London, South Kensington, London SW7 2AZ, UK
    Nat Commun 4:1812. 2013
    ....
  27. ncbi request reprint Plasmodium berghei: routine production of pure gametocytes, extracellular gametes, zygotes, and ookinetes
    M C Rodriguez
    Infection and Immunity Section, Imperial College of Science Technology and Medicine, Department of Biology, Alexander Fleming Building, Imperial College Road, London SW7 2AZ, UK
    Exp Parasitol 101:73-6. 2002
  28. ncbi request reprint Identification of differentially regulated genes of Plasmodium by suppression subtractive hybridization
    J T Dessens
    Imperial College of Science, Technology and Medicine, Department of Biology, Sir Alexander Fleming Building, Imperial College Road, London, UK SW7 2AZ
    Parasitol Today 16:354-6. 2000
    ....
  29. ncbi request reprint Molecular interactions between Plasmodium and its insect vectors
    R E Sinden
    Biological Sciences Department, Imperial College of Science, Technology and Medicine, London SW7 2AZ, UK
    Cell Microbiol 4:713-24. 2002
    ..It will conclude by trying to explain why in the opinion of this reviewer understanding malaria-mosquito interactions may be critical to our future attempts to limit a disease of growing global importance...
  30. pmc Paternal effect of the nuclear formin-like protein MISFIT on Plasmodium development in the mosquito vector
    Ellen S C Bushell
    Department of Life Sciences, Imperial College London, London, UK
    PLoS Pathog 5:e1000539. 2009
    ..This study offers a new perspective for understanding the male contribution to malaria parasite development in the mosquito vector...
  31. pmc The development and routine application of high-density exoerythrocytic-stage cultures of Plasmodium berghei
    R E Sinden
    Department of Pure and Applied Biology, Imperial College, London, England
    Bull World Health Organ 68:115-25. 1990
    ..Some of the new avenues of research made feasible by the high-density cultures, e.g., direct immunization to produce monoclonal antibodies and biochemical studies are discussed...
  32. ncbi request reprint Factors regulating natural transmission of Plasmodium berghei to the mosquito vector, and the cloning of a transmission-blocking immunogen
    R E Sinden
    Department of Biology, Imperial College, London, U K
    Parassitologia 35:107-12. 1993
    ..5%), 4 EGF-like domains and hydrophobic amino-'signal'--and carboxyl-'anchor' sequences. The encoding gene is on chromosome 5 and is found also in P. chabaudi, P. yoelii and P. vinckei...
  33. ncbi request reprint Plasmodium differentiation in the mosquito
    R E Sinden
    Department of Biology, Imperial College of Science, Technology and Medicine, London, UK
    Parassitologia 41:139-48. 1999
    ..The potential exploitation of these interactions as possible mechanisms for intervention will be discussed...
  34. pmc Plasmodium berghei calcium-dependent protein kinase 3 is required for ookinete gliding motility and mosquito midgut invasion
    Inga Siden-Kiamos
    Division of Cell and Molecular Biology, Sir Alexander Fleming Building, Imperial College London, London SW7 2AZ, UK
    Mol Microbiol 60:1355-63. 2006
    ....
  35. ncbi request reprint Transcriptome analysis of Anopheles stephensi-Plasmodium berghei interactions
    Xiaojin Xu
    Department of Biological Sciences, Imperial College, London SW7 2AZ, UK
    Mol Biochem Parasitol 142:76-87. 2005
    ..Intriguing transcription patterns for highly variable Plasmodium surface antigens may indicate parasite strategies to avoid recognition by the mosquito's immune surveillance system...
  36. pmc A Plasmodium falciparum strain expressing GFP throughout the parasite's life-cycle
    Arthur M Talman
    Division of Cell and Molecular Biology, Imperial College London, London, United Kingdom
    PLoS ONE 5:e9156. 2010
    ....
  37. ncbi request reprint Functional characterization of an LCCL-lectin domain containing protein family in Plasmodium berghei
    Holly E Trueman
    Immunology and Infection Section, Department of Biological Sciences, Imperial College London, South Kensington, London SW7 2AZ, UK
    J Parasitol 90:1062-71. 2004
    ..The mutant is not rescued by coparasitization of mosquitoes with a mixture Pblap1+ and Pblap1- homokaryotic oocysts...
  38. pmc A DNA prime-modified vaccinia virus ankara boost vaccine encoding thrombospondin-related adhesion protein but not circumsporozoite protein partially protects healthy malaria-naive adults against Plasmodium falciparum sporozoite challenge
    S J Dunachie
    University of Oxford, Nuffield Department of Clinical Medicine, Centre for Clinical Vaccinology and Tropical Medicine, Churchill Hospital, Old Rd, Headington, Oxford OX3 7LJ, United Kingdom
    Infect Immun 74:5933-42. 2006
    ..033). No protection was observed in the DDM-CS group. Prime-boost vaccination with DNA and MVA encoding ME-TRAP but not CS resulted in partial protection against P. falciparum sporozoite challenge in the present study...
  39. doi request reprint Malaria, sexual development and transmission: retrospect and prospect
    R E Sinden
    Department of Life Sciences, Imperial College, London, UK
    Parasitology 136:1427-34. 2009
    ..This personal view looks back at the significant progress made, and forward to the challenges of the future, focusing on work on sexual development...
  40. pmc Knockout of the rodent malaria parasite chitinase pbCHT1 reduces infectivity to mosquitoes
    J T Dessens
    Department of Biology, Imperial College of Science, Technology, and Medicine, London SW7 2AZ, United Kingdom
    Infect Immun 69:4041-7. 2001
    ..Taken together, these findings suggest a fundamental divergence among rodent, avian, and human malaria parasite chitinases, with implications for the evolution of Plasmodium-mosquito interactions...
  41. pmc A liver-stage specific antigen of P. berghei identified by a monoclonal antibody
    L Winger
    Department of Pure and Applied Biology, Imperial College, London, England
    Bull World Health Organ 68:172-7. 1990
    ..Passive immunization studies using this antibody suggest that it may influence the course of sporozoite-induced infections...
  42. pmc Proteomic comparison of four Eimeria tenella life-cycle stages: unsporulated oocyst, sporulated oocyst, sporozoite and second-generation merozoite
    Kalpana Lal
    The Division of Cell and Molecular Biology, Imperial College London, London, UK
    Proteomics 9:4566-76. 2009
    ..We find a greater abundance of protein linked to transcription, protein synthesis and cell cycle in merozoites than in sporozoites, which may be residual protein from the preceding massive replication during schizogony...
  43. ncbi request reprint SOAP, a novel malaria ookinete protein involved in mosquito midgut invasion and oocyst development
    Johannes T Dessens
    Department of Biological Sciences, Sir Alexander Fleming Building, Imperial College London, South Kensington Campus, SW7 2AZ, UK
    Mol Microbiol 49:319-29. 2003
    ..These results identify SOAP as a key molecule for ookinete-to-oocyst differentiation in mosquitoes...
  44. pmc Characterisation of Plasmodium invasive organelles; an ookinete microneme proteome
    Kalpana Lal
    Division of Cell and Molecular Biology, Imperial College London, UK
    Proteomics 9:1142-51. 2009
    ..Previously uncharacterised proteins comprise the largest functional group of the microneme proteome and will include secreted proteins important to invasion...
  45. pmc Reverse genetics screen identifies six proteins important for malaria development in the mosquito
    Andrea Ecker
    Division of Cell and Molecular Biology, Imperial College London, London SW7 2AZ, UK
    Mol Microbiol 70:209-20. 2008
    ....
  46. ncbi request reprint Persistence of atovaquone in human sera following treatment: inhibition of Plasmodium falciparum development in vivo and in vitro
    Geoff A Butcher
    Department of Biologic Sciences, Imperial College of Science Technology and Medicine, London, UK
    Am J Trop Med Hyg 68:111-4. 2003
    ..Also, persistence of atovaquone in plasma at low concentrations for long periods may increase the risk of resistant parasites arising...
  47. doi request reprint Plasmodium male development gene-1 (mdv-1) is important for female sexual development and identifies a polarised plasma membrane during zygote development
    Kalpana Lal
    The Division of Cell and Molecular Biology, Imperial College London, London, UK
    Int J Parasitol 39:755-61. 2009
    ..In the fully developed ookinete MDV-1 is localised to the posterior pole. In vivo, the knock-out parasites demonstrate a phenotype in which there is a 90% reduction of parasite transmission to oocysts in mosquitoes...
  48. doi request reprint Plasmodium berghei HAP2 induces strong malaria transmission-blocking immunity in vivo and in vitro
    Andrew M Blagborough
    Division of Cell and Molecular Biology, Sir Alexander Fleming Building, Imperial College London, South Kensington, London SW7 2AZ, UK
    Vaccine 27:5187-94. 2009
    ..1%, and prevalence of in vivo infection by up to 34%. Inhibition is dose dependent. These results indicate that HAP2 should be considered as a potential target for any future anti-malarial transmission-blocking vaccine...
  49. ncbi request reprint A malaria scavenger receptor-like protein essential for parasite development
    Charles Claudianos
    Department of Biological Sciences, Imperial College of Science Technology and Medicine, London, UK
    Mol Microbiol 45:1473-84. 2002
    ..berghei results in parasites that form normal numbers of oocysts, but fail to produce any sporozoites. We suggest that, in addition to a role in sporogonic development, PxSR may have a multiplicity of functions...
  50. ncbi request reprint Isonicotinic acid hydrazide: an anti-tuberculosis drug inhibits malarial transmission in the mosquito gut
    Meiji Arai
    Department of Biological Sciences, Imperial College of Science, Technology, and Medicine, London SW7 2AZ, UK
    Exp Parasitol 106:30-6. 2004
    ..Further elucidation of the mechanism how INH inhibits parasite development specifically on mosquito stages may allow us to identify new targets for malaria control strategy...
  51. pmc Plasmodium berghei: plasmodium perforin-like protein 5 is required for mosquito midgut invasion in Anopheles stephensi
    Andrea Ecker
    Division of Cell and Molecular Biology, Faculty of Natural Sciences, Imperial College London, Sir Alexander Fleming Building, Imperial College Road, London, UK
    Exp Parasitol 116:504-8. 2007
    ..The indistinguishable phenotypes of Deltapplp5 and Deltapplp3 ookinetes strongly suggest that these two proteins contribute to a common process...
  52. doi request reprint Intranasal and intramuscular immunization with Baculovirus Dual Expression System-based Pvs25 vaccine substantially blocks Plasmodium vivax transmission
    Andrew M Blagborough
    Division of Cell and Molecular Biology, Department of Life Sciences, Sir Alexander Fleming Building, Imperial College London, Imperial College Road, London SW7 2AZ, UK
    Vaccine 28:6014-20. 2010
    ....
  53. doi request reprint The temporal dynamics of Plasmodium density through the sporogonic cycle within Anopheles mosquitoes
    Emma J Dawes
    Department of Infectious Disease Epidemiology, Faculty of Medicine, Imperial College London, St Mary s Campus, Norfolk Place, London W2 1PG, UK
    Trans R Soc Trop Med Hyg 103:1197-8. 2009
    ..This has implications for the design of parasite-mosquito interface studies and the development of transmission-blocking strategies...
  54. ncbi request reprint Maintenance of the Plasmodium berghei life cycle
    Robert E Sinden
    Department of Biology, Imperial College of Science, Technology and Medicine, London, UK
    Methods Mol Med 72:25-40. 2002
  55. pmc A malaria membrane skeletal protein is essential for normal morphogenesis, motility, and infectivity of sporozoites
    Emad I Khater
    Department of Biological Sciences, Imperail College London, London SW7 2AZ, England, UK
    J Cell Biol 167:425-32. 2004
    ..We identify a family of proteins related to PbIMC1a in Plasmodium and other apicomplexan parasites. These results provide new functional insight in the role of membrane skeletons in apicomplexan parasite biology...
  56. doi request reprint Localisation and timing of expression of putative Plasmodium berghei rhoptry proteins in merozoites and sporozoites
    Marta Tufet-Bayona
    The Malaria Centre, and the Division of Cell and Molecular Biology, Imperial College London, London, UK
    Mol Biochem Parasitol 166:22-31. 2009
    ....
  57. ncbi request reprint LCCL proteins of apicomplexan parasites
    Johannes T Dessens
    Department of Biological Sciences, Imperial College London, Sir Alexander Fleming Building, South Kensington Campus, Imperial College Road, London, SW7 2AZ, UK
    Trends Parasitol 20:102-8. 2004
    ..Here, the current data and literature on the members of this protein family are reviewed, with a discussion on their possible roles in host-parasite interaction...
  58. ncbi request reprint Generation of gene targeting constructs for Plasmodium berghei by a PCR-based method amenable to high throughput applications
    Andrea Ecker
    Division of Cell and Molecular Biology, Imperial College London, Exhibition Road, London SW7 2AZ, UK
    Mol Biochem Parasitol 145:265-8. 2006
  59. ncbi request reprint Genome sequence and comparative analysis of the model rodent malaria parasite Plasmodium yoelii yoelii
    Jane M Carlton
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, Maryland 20850, USA
    Nature 419:512-9. 2002
    ..This is the first genome sequence of a model eukaryotic parasite, and it provides insight into the use of such systems in the modelling of Plasmodium biology and disease...
  60. ncbi request reprint Close association of invading Plasmodium berghei and beta integrin in the Anopheles gambiae midgut
    Vasso Mahairaki
    Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, Heraklion, Crete, Greece
    Arch Insect Biochem Physiol 60:13-9. 2005
    ..This intimate association suggests a specific role of beta integrin in the invasion process...
  61. ncbi request reprint Complete development of mosquito phases of the malaria parasite in vitro
    Ebtesam M Al-Olayan
    Centre for Applied Entomology and Parasitology, School of Life Sciences, Keele University, Staffordshire ST5 5BG, UK
    Science 295:677-9. 2002
    ..Sporozoite production required the presence of para-aminobenzoic acid. The entire life cycle of P. berghei, a useful model malaria parasite, can now be achieved in vitro...
  62. ncbi request reprint Safety, immunogenicity and efficacy of a pre-erythrocytic malaria candidate vaccine, ICC-1132 formulated in Seppic ISA 720
    Michael Walther
    Nuffield Department of Clinical Medicine, Centre for Clinical Vaccinology and Tropical Medicine, Oxford University, Old Road, Headington, Oxford OX3 7LJ, UK
    Vaccine 23:857-64. 2005
    ..There was no evidence of protection from experimental challenge with P. falciparum sporozoites. Other formulations and/or multi-dose regimens will be required to enhance the immunogenicity and efficacy of ICC-1132...
  63. ncbi request reprint A comprehensive survey of the Plasmodium life cycle by genomic, transcriptomic, and proteomic analyses
    Neil Hall
    Pathogen Sequencing Unit, Wellcome Trust Sanger Institute, Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Science 307:82-6. 2005
    ..We observed posttranscriptional gene silencing through translational repression of messenger RNA during sexual development, and a 47-base 3' untranslated region motif is implicated in this process...
  64. ncbi request reprint Direct processing and presentation of antigen from malaria sporozoites by professional antigen-presenting cells in the induction of CD8 T-cell responses
    Magdalena Plebanski
    Nuffield Department of Medicine, University of Oxford, John Radcliffe Hospital, UK
    Immunol Cell Biol 83:307-12. 2005
    ..The induction of sporozoite-specific CD8 T-cell responses without the need for liver stage infection identifies a potentially important mechanism in the development of pre-erythrocytic immunity...
  65. ncbi request reprint Plasmodium berghei ookinetes bind to Anopheles gambiae and Drosophila melanogaster annexins
    Michalis Kotsyfakis
    Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, Vassilika Vouton, PO Box 1527, 71110 Heraklion, Crete, Greece
    Mol Microbiol 57:171-9. 2005
    ..Finally, inclusion of antiannexin antisera in a mosquito blood meal impaired parasite development, suggesting a facilitating role for annexins in the infection of the mosquito by Plasmodium...
  66. ncbi request reprint A Plasmodium berghei reference line that constitutively expresses GFP at a high level throughout the complete life cycle
    Blandine Franke-Fayard
    Department of Parasitology, Leiden University Medical Centre, Postbus 9600 RC, The Netherlands
    Mol Biochem Parasitol 137:23-33. 2004
    ..With the recent development of a second, independent selectable marker in P. berghei, PbGFPCON is a useful tool to investigate the effect of further genetic modifications on host-parasite interactions...
  67. pmc The role of metacaspase 1 in Plasmodium berghei development and apoptosis
    Ludovic Le Chat
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, United Kingdom
    Mol Biochem Parasitol 153:41-7. 2007
    ..We failed to detect an apparent loss-of-function phenotype, suggesting that PbMC1 constitutes a functionally redundant gene. We discuss these findings in the context of two other putative Plasmodium metacaspases that we describe here...
  68. pmc Minimum requirements for ookinete to oocyst transformation in Plasmodium
    Victoria Carter
    Centre for Applied Entomology and Parasitology, Institute for Science and Technology in Medicine, Huxley Building, Keele University, Staffordshire ST5 5BG, UK
    Int J Parasitol 37:1221-32. 2007
    ..8 to 11.4microm by day 5 with 84% viability. We conclude that ookinete transformation is mediated by bicarbonate and occurs in a similar manner to the differentiation of sporozoite to the hepatic stage...
  69. pmc Hemolytic C-type lectin CEL-III from sea cucumber expressed in transgenic mosquitoes impairs malaria parasite development
    Shigeto Yoshida
    Division of Medical Zoology, Department of Infection and Immunity, Jichi Medical University, Tochigi, Japan
    PLoS Pathog 3:e192. 2007
    ....
  70. pmc Determining the protein repertoire of Cryptosporidium parvum sporozoites
    Sanya J Sanderson
    Departments of Pre clinical Veterinary Science and Veterinary Pathology, Faculty of Veterinary Science, University of Liverpool, Liverpool, UK
    Proteomics 8:1398-414. 2008
    ..For approximately half the proteome there was limited functional and structural information, highlighting the limitations in the current understanding of Cryptosporidium biology...
  71. ncbi request reprint Enhanced T-cell immunogenicity of plasmid DNA vaccines boosted by recombinant modified vaccinia virus Ankara in humans
    Samuel J McConkey
    Nuffield Department of Medicine, University of Oxford, John Radcliffe Hospital, Oxford, OX3 9DU, UK
    Nat Med 9:729-35. 2003
    ..Such heterologous prime-boost immunization approaches may provide a basis for preventative and therapeutic vaccination in humans...
  72. pmc Safety, immunogenicity, and efficacy of prime-boost immunization with recombinant poxvirus FP9 and modified vaccinia virus Ankara encoding the full-length Plasmodium falciparum circumsporozoite protein
    Michael Walther
    Centre for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Clinical Medicine, Oxford University, United Kingdom
    Infect Immun 74:2706-16. 2006
    ..Vaccines containing this antigen proved safe and induced modest immune responses but showed no evidence of efficacy in a sporozoite challenge...
  73. ncbi request reprint A clinical trial of prime-boost immunisation with the candidate malaria vaccines RTS,S/AS02A and MVA-CS
    Susanna J Dunachie
    Centre for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Medicine, University of Oxford, Churchill Hospital, Oxford OX3 7LJ, UK
    Vaccine 24:2850-9. 2006
    ..Protection against 3D7 Plasmodium falciparum sporozoite challenge 4 weeks after the final vaccination was equal for both regimens at 33% (95% C.I. 4.3-77.7%), with one subject remaining fully protected on rechallenge at 5 months...
  74. ncbi request reprint Upregulation of TGF-beta, FOXP3, and CD4+CD25+ regulatory T cells correlates with more rapid parasite growth in human malaria infection
    Michael Walther
    Center for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Clinical Medicine, Oxford University, Churchill Hospital, Oxford OX3 7LJ, United Kingdom
    Immunity 23:287-96. 2005
    ..P. falciparum-mediated induction of regulatory T cells may represent a parasite-specific virulence factor...
  75. pmc The conserved plant sterility gene HAP2 functions after attachment of fusogenic membranes in Chlamydomonas and Plasmodium gametes
    Yanjie Liu
    Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Genes Dev 22:1051-68. 2008
    ..Membrane dye experiments show that HAP2 is essential for membrane merger. Thus, in two distantly related eukaryotes, species-limited proteins govern access to a conserved protein essential for membrane fusion...
  76. ncbi request reprint Azadirachtin disrupts formation of organised microtubule arrays during microgametogenesis of Plasmodium berghei
    Oliver Billker
    Department of Biological Sciences, Imperial College of Science, Technology and Medicine, London SW7 2AZ UK
    J Eukaryot Microbiol 49:489-97. 2002
    ..Our observations suggest that azadarachtin specifically disrupts the patterning of microtubules into more complex structures, such as mitotic spindles and axonemes...
  77. ncbi request reprint A proteomic view of the Plasmodium falciparum life cycle
    Laurence Florens
    Department of Cell Biology, The Scripps Research Institute, SR 11, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Nature 419:520-6. 2002
    ..The detection of chromosomal clusters encoding co-expressed proteins suggested a potential mechanism for controlling gene expression...