Nicholas J Severs

Summary

Affiliation: Imperial College
Country: UK

Publications

  1. ncbi request reprint Up-regulation of connexin43 correlates with increased synthetic activity and enhanced contractile differentiation in TGF-beta-treated human aortic smooth muscle cells
    Aisha Rama
    Cardiac Medicine, National Heart and Lung Institute Imperial College, Guy Scadding Building, Dovehouse Street, London SW3 6LY, UK
    Eur J Cell Biol 85:375-86. 2006
  2. doi request reprint Coexpression of connexin 45 with connexin 43 decreases gap junction size
    Katharina Grikscheit
    Department of Cardiac Medicine, National Heart and Lung Institute, London, United Kingdom
    Cell Commun Adhes 15:185-93. 2008
  3. ncbi request reprint Alterations in cardiac connexin expression in cardiomyopathies
    Nicholas J Severs
    National Heart and Lung Institute, Imperial College London, UK
    Adv Cardiol 42:228-42. 2006
  4. ncbi request reprint Freeze-fracture electron microscopy
    Nicholas J Severs
    Imperial College London, National Heart and Lung Institute, Dovehouse Street, London SW3 6LY, UK
    Nat Protoc 2:547-76. 2007
  5. ncbi request reprint Gap junction alterations in human cardiac disease
    Nicholas J Severs
    Cardiac Medicine, National Heart and Lung Institute, Imperial College Faculty of Medicine, Guy Scadding Building, Dovehouse Street, London SW3 6LY, UK
    Cardiovasc Res 62:368-77. 2004
  6. pmc Remodelling of gap junctions and connexin expression in diseased myocardium
    Nicholas J Severs
    National Heart and Lung Institute, Imperial College London, Dovehouse Street, London SW3 6LY, UK
    Cardiovasc Res 80:9-19. 2008
  7. ncbi request reprint Relationship of connexin43 expression to phenotypic modulation in cultured human aortic smooth muscle cells
    Tsutomu Matsushita
    Cardiac Medicine, National Heart and Lung Institute Imperial College London, Guy Scadding Building, Dovehouse Street, London SW3 6LY, UK
    Eur J Cell Biol 86:617-28. 2007
  8. ncbi request reprint Gap junction remodelling in human heart failure is associated with increased interaction of connexin43 with ZO-1
    Alexandra F Bruce
    National Heart and Lung Institute, Imperial College London, Guy Scadding Building, London, UK
    Cardiovasc Res 77:757-65. 2008
  9. ncbi request reprint Remodelling of gap junctions and connexin expression in heart disease
    Nicholas J Severs
    National Heart and Lung Institute, Faculty of Medicine, Imperial College, Royal Brompton Hospital, Sydney Street, London SW3 6NP, UK
    Biochim Biophys Acta 1662:138-48. 2004
  10. ncbi request reprint Development of a cell model for functional and structural analysis of connexin co-expression: achieving homogeneous and inducible expression of multiple connexins in stable transfectants
    Deborah Halliday
    National Heart and Lung Institute, Faculty of Medicine, Imperial College London, London, United Kingdom
    Cell Commun Adhes 10:311-7. 2003

Collaborators

Detail Information

Publications34

  1. ncbi request reprint Up-regulation of connexin43 correlates with increased synthetic activity and enhanced contractile differentiation in TGF-beta-treated human aortic smooth muscle cells
    Aisha Rama
    Cardiac Medicine, National Heart and Lung Institute Imperial College, Guy Scadding Building, Dovehouse Street, London SW3 6LY, UK
    Eur J Cell Biol 85:375-86. 2006
    ..We conclude that in human aortic SMCs, TGF-beta1 treatment leads to up-regulation of Cx43-mediated gap-junctional communication and increased synthetic activity yet, somewhat paradoxically, also enhanced contractile differentiation...
  2. doi request reprint Coexpression of connexin 45 with connexin 43 decreases gap junction size
    Katharina Grikscheit
    Department of Cardiac Medicine, National Heart and Lung Institute, London, United Kingdom
    Cell Commun Adhes 15:185-93. 2008
    ..In contrast, coexpression of Cx40 with Cx43 does not lead to any change in gap junction size. These results are consistent with the idea that increased Cx45 expression in the failing ventricle contributes to decreased gap junction size...
  3. ncbi request reprint Alterations in cardiac connexin expression in cardiomyopathies
    Nicholas J Severs
    National Heart and Lung Institute, Imperial College London, UK
    Adv Cardiol 42:228-42. 2006
    ....
  4. ncbi request reprint Freeze-fracture electron microscopy
    Nicholas J Severs
    Imperial College London, National Heart and Lung Institute, Dovehouse Street, London SW3 6LY, UK
    Nat Protoc 2:547-76. 2007
    ..Images provided by freeze fracture and related techniques have profoundly shaped our understanding of the functional morphology of the cell...
  5. ncbi request reprint Gap junction alterations in human cardiac disease
    Nicholas J Severs
    Cardiac Medicine, National Heart and Lung Institute, Imperial College Faculty of Medicine, Guy Scadding Building, Dovehouse Street, London SW3 6LY, UK
    Cardiovasc Res 62:368-77. 2004
    ....
  6. pmc Remodelling of gap junctions and connexin expression in diseased myocardium
    Nicholas J Severs
    National Heart and Lung Institute, Imperial College London, Dovehouse Street, London SW3 6LY, UK
    Cardiovasc Res 80:9-19. 2008
    ....
  7. ncbi request reprint Relationship of connexin43 expression to phenotypic modulation in cultured human aortic smooth muscle cells
    Tsutomu Matsushita
    Cardiac Medicine, National Heart and Lung Institute Imperial College London, Guy Scadding Building, Dovehouse Street, London SW3 6LY, UK
    Eur J Cell Biol 86:617-28. 2007
    ..We conclude that Cx43 levels, though having common correlates, are not exclusively linked to the cell phenotype or the state of growth...
  8. ncbi request reprint Gap junction remodelling in human heart failure is associated with increased interaction of connexin43 with ZO-1
    Alexandra F Bruce
    National Heart and Lung Institute, Imperial College London, Guy Scadding Building, London, UK
    Cardiovasc Res 77:757-65. 2008
    ..We therefore examined the hypothesis that ZO-1 contributes to reduced expression of Cx43 gap junctions in congestive heart failure...
  9. ncbi request reprint Remodelling of gap junctions and connexin expression in heart disease
    Nicholas J Severs
    National Heart and Lung Institute, Faculty of Medicine, Imperial College, Royal Brompton Hospital, Sydney Street, London SW3 6NP, UK
    Biochim Biophys Acta 1662:138-48. 2004
    ....
  10. ncbi request reprint Development of a cell model for functional and structural analysis of connexin co-expression: achieving homogeneous and inducible expression of multiple connexins in stable transfectants
    Deborah Halliday
    National Heart and Lung Institute, Faculty of Medicine, Imperial College London, London, United Kingdom
    Cell Commun Adhes 10:311-7. 2003
    ..The cell model is now at a suitable stage of development for investigation of the functional correlates of the distinctive connexin co-expression found in different regions of the heart...
  11. ncbi request reprint High-resolution en-face visualization of the cardiomyocyte plasma membrane reveals distinctive distributions of spectrin and dystrophin
    Shirley A Stevenson
    Faculty of Medicine, Imperial College London, National Heart and Lung Institute, Guy Scadding Building, UK
    Eur J Cell Biol 84:961-71. 2005
    ..g., in structural support to the plasma membrane) may exist between them, the two systems may each have additional distinctive roles...
  12. ncbi request reprint Comparison of connexin expression patterns in the developing mouse heart and human foetal heart
    Steven R Coppen
    Cardiac Medicine, National Heart and Lung Institute, Faculty of Medicine, Imperial College, Royal Brompton Hospital, London, UK
    Mol Cell Biochem 242:121-7. 2003
    ..We conclude that, at least in some key aspects, the pattern of connexin expression in the developing mouse heart parallels that found in the human embryonic heart...
  13. ncbi request reprint Human atrial conduction and arrhythmogenesis correlates with conformational exposure of specific epitopes on the connexin40 carboxyl tail
    Prapa Kanagaratnam
    National Heart and Lung Institute, London, UK
    J Mol Cell Cardiol 40:675-87. 2006
    ....
  14. ncbi request reprint Localisation of SCN10A gene product Na(v)1.8 and novel pain-related ion channels in human heart
    Paul Facer
    Department of Clinical Neuroscience, Hammersmith Hospital, Faculty of Medicine, Imperial College London, London, UK
    Int Heart J 52:146-52. 2011
    ..We conclude that sodium channel Na(v)1.8 is present in sensory nerves and cardiomyocytes of human heart. Na(v)1.8 and other pain channels provide new targets for the understanding and treatment of cardiac pain and dysrhythmia...
  15. ncbi request reprint Relative expression of immunolocalized connexins 40 and 43 correlates with human atrial conduction properties
    Prapa Kanagaratnam
    Heart and Lung Division of Imperial College School of Medicine, London, United Kingdom
    J Am Coll Cardiol 39:116-23. 2002
    ..The aim of this study was to determine the relationship between immunolocalized gap-junctional proteins and human atrial conduction...
  16. ncbi request reprint Remodeling of myocardial sleeve and gap junctions in canine superior vena cava after rapid pacing
    Hung I Yeh
    Department of Internal Medicine and Medical Research Mackay Memorial Hospital, Mackay Medicine Nursing and Management College, Taipei Medical University, 92, Sec 2, Chung San North Road, 10449, Taipei, Taiwan
    Basic Res Cardiol 101:269-80. 2006
    ..We studied the response of the superior vena cava (SVC) myocardial sleeve to atrial fibrillation (AF)...
  17. ncbi request reprint Gap junction channels and cardiac impulse propagation
    Thomas Desplantez
    Institute of Physiology, University of Bern, Buhlplatz 5, Bern, Switzerland
    J Membr Biol 218:13-28. 2007
    ..The data predict facilitation of orthodromic propagation...
  18. ncbi request reprint Upregulation of connexin43 gap junctions between neointimal smooth muscle cells
    Gabriele Plenz
    Institute for Arteriosclerosis Research, Munster, Germany
    Eur J Cell Biol 83:521-30. 2004
    ..We conclude that the enhanced gap junction formation may contribute to the coordination of the response of SMC after secondary injury, particularly in the early phase of restenosis...
  19. ncbi request reprint Alteration of gap junctions and connexins in the right atrial appendage during cardiopulmonary bypass
    Hung I Yeh
    Departments of Cardiac Medicine and Medical Research, Mackay Memorial Hospital, Taipei Medical University, Taipei, Taiwan
    J Thorac Cardiovasc Surg 124:1106-12. 2002
    ..We investigated the influence of cardiopulmonary bypass on cardiomyocyte gap junctions and connexins...
  20. ncbi request reprint Gap junction remodeling in heart failure
    Nicholas J Severs
    National Heart and Lung Institute, Faculty of Medicine, Imperial College, London, United Kingdom
    J Card Fail 8:S293-9. 2002
    ..Recent evidence from studies in experimental animals strengthens the case that gap junction remodeling is a key determinant of the proarrhythmic substrate in the diseased heart...
  21. pmc The carboxy terminal domain of connexin43: from molecular regulation of the gap junction channel to supramolecular organization of the intercalated disk
    Nicholas J Severs
    Circ Res 101:1213-5. 2007
  22. doi request reprint Freeze-fracture cytochemistry in cell biology
    Nicholas J Severs
    Imperial College London, National Heart and Lung Division, London, United Kingdom
    Methods Cell Biol 88:181-204. 2008
    ..Examples of how these techniques have contributed to our understanding of cardiovascular cell function in health and disease are discussed...
  23. ncbi request reprint Adipophilin-enriched domains in the ER membrane are sites of lipid droplet biogenesis
    Horst Robenek
    Leibniz Institute for Arteriosclerosis Research, University of Munster, Domagkstr 3, 48149 Munster, Germany
    J Cell Sci 119:4215-24. 2006
    ..We identify these structures as sites at which lipids and adipophilin are transferred from ER membranes to lipid droplets. These findings call for a re-evaluation of the prevailing hypothesis of lipid droplet biogenesis...
  24. pmc Butyrophilin controls milk fat globule secretion
    Horst Robenek
    Leibniz Institute for Arteriosclerosis Research, University of Munster, 48149 Munster, Germany
    Proc Natl Acad Sci U S A 103:10385-90. 2006
    ....
  25. ncbi request reprint Lipid droplets gain PAT family proteins by interaction with specialized plasma membrane domains
    Horst Robenek
    Department of Cell Biology and Ultrastructure Research, Leibniz Institute for Arteriosclerosis Research, University of Munster, Munster, Germany
    J Biol Chem 280:26330-8. 2005
    ..Lipid droplets appear to acquire their PAT proteins by interaction with plasma membrane domains enriched in these proteins...
  26. ncbi request reprint Expression and localization of tissue factor pathway inhibitor-2 in normal and atherosclerotic human vessels
    James T B Crawley
    Thrombosis Research Institute, London, England
    Arterioscler Thromb Vasc Biol 22:218-24. 2002
    ....
  27. ncbi request reprint Re: The sinoatrial node, connexin distribution patterns and specific immunodetection of connexin45
    Steven R Coppen
    Cardiovasc Res 53:1043-5; author reply 1046. 2002
  28. ncbi request reprint Diversity of connexin expression patterns in the atrioventricular node: vestigial consequence or functional specialization?
    Steven R Coppen
    National Heart and Lung Institute, Faculty of Medicine, Imperial College, London, United Kingdom
    J Cardiovasc Electrophysiol 13:625-6. 2002
  29. ncbi request reprint Reduced expression of endothelial connexin37 and connexin40 in hyperlipidemic mice: recovery of connexin37 after 7-day simvastatin treatment
    Hung I Yeh
    Mackay Memorial Hospital, Mackay Junior College of Nursing, Taipei Medical University, Taipei, Taiwan
    Arterioscler Thromb Vasc Biol 23:1391-7. 2003
    ..We sought to clarify the response of endothelial connexins to hyperlipidemia and lipid-lowering therapy...
  30. ncbi request reprint Three-dimensional reconstruction of the rabbit atrioventricular conduction axis by combining histological, desmin, and connexin mapping data
    Yu Shien Ko
    First Cardiovascular Division, Chang Gung Memorial Hospital, Taipei, Taiwan, Republic of China
    Circulation 109:1172-9. 2004
    ....
  31. ncbi request reprint Lipids partition caveolin-1 from ER membranes into lipid droplets: updating the model of lipid droplet biogenesis
    Mirko J Robenek
    University Children s Hospital, University of Munster, Munster, Germany
    FASEB J 18:866-8. 2004
    ....
  32. ncbi request reprint Spontaneous activity and electrical coupling in human detrusor smooth muscle: implications for detrusor overactivity?
    Christopher H Fry
    Institute of Urology and Nephrology, London, United Kingdom
    Urology 63:3-10. 2004
    ..The data do imply that myogenic defects can contribute to abnormal bladder function and, thus, suggest several new targeted drug models that should be explored...
  33. ncbi request reprint Relationship between connexins and atrial activation during human atrial fibrillation
    Prapa Kanagaratnam
    Heart and Lung Division of Imperial College School of Medicine, and St Mary s Hospital, London, United Kingdom
    J Cardiovasc Electrophysiol 15:206-16. 2004
    ..We hypothesized that atrial activation pattern during AF is related to connexin expression and that this relationship is altered by AF-induced remodeling in the fibrillating atria of chronic AF...
  34. ncbi request reprint Heterogeneous expression of connexins in rabbit sinoatrial node cells: correlation between connexin isotype and cell size
    Haruo Honjo
    Research Institute of Environmental Medicine, Nagoya University, Furo cho, Chikusa ku, Nagoya 464 8601, Japan
    Cardiovasc Res 53:89-96. 2002
    ..The purpose of this study was to identify the connexin isotypes expressed by sinoatrial node cells and to analyse the density of connexins in relation to cell size...