Jorg Schumacher

Summary

Affiliation: Imperial College
Country: UK

Publications

  1. ncbi request reprint Sensor I threonine of the AAA+ ATPase transcriptional activator PspF is involved in coupling nucleotide triphosphate hydrolysis to the restructuring of sigma 54-RNA polymerase
    Jorg Schumacher
    Division of Biology, Imperial College London, London SW7 2AZ, UK
    J Biol Chem 282:9825-33. 2007
  2. ncbi request reprint Structures and organisation of AAA+ enhancer binding proteins in transcriptional activation
    Jorg Schumacher
    Division of Biology, Imperial College London, London, SW7 2AZ, UK
    J Struct Biol 156:190-9. 2006
  3. doi request reprint Mechanism of homotropic control to coordinate hydrolysis in a hexameric AAA+ ring ATPase
    Jorg Schumacher
    Division of Biology, Imperial College London, London SW7 2AZ, UK
    J Mol Biol 381:1-12. 2008
  4. pmc The ATP hydrolyzing transcription activator phage shock protein F of Escherichia coli: identifying a surface that binds sigma 54
    Patricia Bordes
    Department of Biological Sciences, Sir Alexander Fleming Building, Imperial College London, South Kensington Campus, London SW7 2AZ, United Kingdom
    Proc Natl Acad Sci U S A 100:2278-83. 2003
  5. pmc Structural insights into the activity of enhancer-binding proteins
    Mathieu Rappas
    Department of Biological Sciences, Imperial College London, London, SW7 2AZ, UK
    Science 307:1972-5. 2005
  6. ncbi request reprint Structural basis of the nucleotide driven conformational changes in the AAA+ domain of transcription activator PspF
    Mathieu Rappas
    Division of Molecular Biosciences, Imperial College London, London SW7 2AZ, UK
    J Mol Biol 357:481-92. 2006
  7. ncbi request reprint ATP-dependent transcriptional activation by bacterial PspF AAA+protein
    Jorg Schumacher
    Department of Biological Sciences, Sir Alexander Fleming Building, Imperial College London, South Kensington Campus, London SW7 2AZ, UK
    J Mol Biol 338:863-75. 2004
  8. ncbi request reprint Heterogeneous nucleotide occupancy stimulates functionality of phage shock protein F, an AAA+ transcriptional activator
    Nicolas Joly
    Division of Biology, Sir Alexander Fleming Building, Imperial College London, London SW7 2AZ, United Kingdom
    J Biol Chem 281:34997-5007. 2006
  9. doi request reprint Dissecting the ATP hydrolysis pathway of bacterial enhancer-binding proteins
    Daniel Bose
    Division of Molecular Bioscience, Faculty of Natural Sciences, Imperial College London, South Kensington, London, SW7 2AZ, U K
    Biochem Soc Trans 36:83-8. 2008
  10. pmc Functional roles of the pre-sensor I insertion sequence in an AAA+ bacterial enhancer binding protein
    Patricia C Burrows
    Department of Life Sciences, Division of Biology, Imperial College London, London, UK
    Mol Microbiol 73:519-33. 2009

Collaborators

Detail Information

Publications14

  1. ncbi request reprint Sensor I threonine of the AAA+ ATPase transcriptional activator PspF is involved in coupling nucleotide triphosphate hydrolysis to the restructuring of sigma 54-RNA polymerase
    Jorg Schumacher
    Division of Biology, Imperial College London, London SW7 2AZ, UK
    J Biol Chem 282:9825-33. 2007
    ..We propose that hydrolysis is relayed via Thr(148) to loop 2 creating motions that provide mechanical force to the GAFTGA loop 1 that contacts sigma(54)...
  2. ncbi request reprint Structures and organisation of AAA+ enhancer binding proteins in transcriptional activation
    Jorg Schumacher
    Division of Biology, Imperial College London, London, SW7 2AZ, UK
    J Struct Biol 156:190-9. 2006
    ..Parallels with the substrate binding elements near the central pore of other AAA+ members are drawn. We propose a structural model of EBPs in complex with a sigma(54)-RNAP-promoter complex...
  3. doi request reprint Mechanism of homotropic control to coordinate hydrolysis in a hexameric AAA+ ring ATPase
    Jorg Schumacher
    Division of Biology, Imperial College London, London SW7 2AZ, UK
    J Mol Biol 381:1-12. 2008
    ..Homotropic coordination is functionally important to remodel the sigma(54) promoter. We propose a structural symmetry-based model for homotropic control in the AAA(+) characteristic ring architecture...
  4. pmc The ATP hydrolyzing transcription activator phage shock protein F of Escherichia coli: identifying a surface that binds sigma 54
    Patricia Bordes
    Department of Biological Sciences, Sir Alexander Fleming Building, Imperial College London, South Kensington Campus, London SW7 2AZ, United Kingdom
    Proc Natl Acad Sci U S A 100:2278-83. 2003
    ....
  5. pmc Structural insights into the activity of enhancer-binding proteins
    Mathieu Rappas
    Department of Biological Sciences, Imperial College London, London, SW7 2AZ, UK
    Science 307:1972-5. 2005
    ..Comparing enhancer-binding structures in different nucleotide states and mutational analysis led us to propose nucleotide-dependent conformational changes that free the loops for association with sigma54...
  6. ncbi request reprint Structural basis of the nucleotide driven conformational changes in the AAA+ domain of transcription activator PspF
    Mathieu Rappas
    Division of Molecular Biosciences, Imperial College London, London SW7 2AZ, UK
    J Mol Biol 357:481-92. 2006
    ..Striking similarities in nucleotide-specific conformational changes and atomic switch exist between PspF and the large T antigen helicase, suggesting conservation in the origin of those events amongst AAA(+) proteins...
  7. ncbi request reprint ATP-dependent transcriptional activation by bacterial PspF AAA+protein
    Jorg Schumacher
    Department of Biological Sciences, Sir Alexander Fleming Building, Imperial College London, South Kensington Campus, London SW7 2AZ, UK
    J Mol Biol 338:863-75. 2004
    ..Substitution of this arginine residue results in nucleotide-independent formation of hexameric rings, structurally linking the putative R-finger and, by inference, a specific nucleotide interaction to the control of PspF oligomerisation...
  8. ncbi request reprint Heterogeneous nucleotide occupancy stimulates functionality of phage shock protein F, an AAA+ transcriptional activator
    Nicolas Joly
    Division of Biology, Sir Alexander Fleming Building, Imperial College London, London SW7 2AZ, United Kingdom
    J Biol Chem 281:34997-5007. 2006
    ..Esigma54 complex provides clear biochemical evidence for heterogeneous nucleotide occupancy in this AAA+ protein. Based on our data, we propose a stochastic nucleotide binding and a coordinated hydrolysis mechanism in PspF1-275 hexamers...
  9. doi request reprint Dissecting the ATP hydrolysis pathway of bacterial enhancer-binding proteins
    Daniel Bose
    Division of Molecular Bioscience, Faculty of Natural Sciences, Imperial College London, South Kensington, London, SW7 2AZ, U K
    Biochem Soc Trans 36:83-8. 2008
    ..In the present article, we review some of the key nucleotides, mutations and techniques used and how they have contributed towards our understanding of the function of bEBPs...
  10. pmc Functional roles of the pre-sensor I insertion sequence in an AAA+ bacterial enhancer binding protein
    Patricia C Burrows
    Department of Life Sciences, Division of Biology, Imperial College London, London, UK
    Mol Microbiol 73:519-33. 2009
    ....
  11. doi request reprint Modus operandi of the bacterial RNA polymerase containing the sigma54 promoter-specificity factor
    Sivaramesh Wigneshweraraj
    Department of Microbiology, Division of Investigative Sciences, Faculty of Medicine and Centre for Molecular Microbiology and Infection, Imperial College London, SW7 2AZ, UK
    Mol Microbiol 68:538-46. 2008
    ..Here, we summarize our current understanding of the interactions the sigma(54) factor makes with the bacterial transcription machinery...
  12. doi request reprint Visualizing the organization and reorganization of transcription complexes for gene expression
    Patricia C Burrows
    Division of Molecular Biosciences, Department of Life Sciences, Imperial College London, London, UK
    Biochem Soc Trans 36:776-9. 2008
    ....
  13. ncbi request reprint Mechanism of action of the Escherichia coli phage shock protein PspA in repression of the AAA family transcription factor PspF
    Sarah Elderkin
    Department of Biological Sciences, Imperial College of Science Technology and Medicine, Biomedical Sciences Building, Imperial College Road, London SW7 2AZ, UK
    J Mol Biol 320:23-37. 2002
    ....
  14. pmc Regulation of the co-evolved HrpR and HrpS AAA+ proteins required for Pseudomonas syringae pathogenicity
    Milija Jovanovic
    Division of Biology, Faculty of Natural Sciences, Sir Alexander Fleming Building, Imperial College London, London SW7 2AZ, UK
    Nat Commun 2:177. 2011
    ..The distinct HrpR and HrpS functionalities suggest how partial paralogue degeneration has potentially led to a novel control mechanism for EBPs and indicate subunit-specific roles for EBPs in σ(54)-RNA polymerase activation...