P J Parker

Summary

Affiliation: Imperial Cancer Research Fund
Country: UK

Publications

  1. ncbi request reprint AGC protein kinase phosphorylation and protein kinase C
    P J Parker
    Protein Phosphorylation Laboratory, Imperial Cancer Research Fund, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Biochem Soc Trans 29:860-3. 2001
  2. ncbi request reprint Rapamycin-sensitive phosphorylation of PKC on a carboxy-terminal site by an atypical PKC complex
    W H Ziegler
    Protein Phosphorylation Laboratory, Imperial Cancer Research Fund, 44 Lincoln s Inn Fields, London, WC2A 3PX, UK
    Curr Biol 9:522-9. 1999
  3. pmc Sac phosphatase domain proteins
    W E Hughes
    Protein Phosphorylation Laboratory, Imperial Cancer Research Fund, 44, Lincoln s Inn Fields, London WC2A 3PX, UK
    Biochem J 350:337-52. 2000
  4. ncbi request reprint Rho GTPase control of protein kinase C-related protein kinase activation by 3-phosphoinositide-dependent protein kinase
    P Flynn
    Imperial Cancer Research Fund, Protein Phosphorylation Laboratory, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
    J Biol Chem 275:11064-70. 2000
  5. ncbi request reprint Imaging protein kinase Calpha activation in cells
    T Ng
    Protein Phosphorylation Laboratory and Cell Biophysics Laboratory, Imperial Cancer Research Fund ICRF, 44 Lincoln s Inn Fields, London, WC2A 3PX, UK
    Science 283:2085-9. 1999
  6. ncbi request reprint p42 MAPK phosphorylates 80 kDa MARCKS at Ser-113
    D C Schönwasser
    Protein Phosphorylation Laboratory, Imperial Cancer Research Fund, London, UK
    FEBS Lett 395:1-5. 1996
  7. ncbi request reprint SAC1 encodes a regulated lipid phosphoinositide phosphatase, defects in which can be suppressed by the homologous Inp52p and Inp53p phosphatases
    W E Hughes
    Protein Phosphorylation, Imperial Cancer Research Fund, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
    J Biol Chem 275:801-8. 2000
  8. ncbi request reprint Protein kinase C isotypes controlled by phosphoinositide 3-kinase through the protein kinase PDK1
    J A Le Good
    Protein Phosphorylation Laboratory, Imperial Cancer Research Fund, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Science 281:2042-5. 1998
  9. ncbi request reprint Regulated binding of the protein kinase C substrate GAP-43 to the V0/C2 region of protein kinase C-delta
    L V Dekker
    Protein Phosphorylation Laboratory, Imperial Cancer Research Fund, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
    J Biol Chem 272:12747-53. 1997
  10. ncbi request reprint PRK1 is targeted to endosomes by the small GTPase, RhoB
    H Mellor
    Protein Phosphorylation Laboratory, Imperial Cancer Research Fund, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
    J Biol Chem 273:4811-4. 1998

Collaborators

Detail Information

Publications51

  1. ncbi request reprint AGC protein kinase phosphorylation and protein kinase C
    P J Parker
    Protein Phosphorylation Laboratory, Imperial Cancer Research Fund, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Biochem Soc Trans 29:860-3. 2001
    ..There are also distinctions relating to the turnover of these phosphorylations providing a further element of specificity...
  2. ncbi request reprint Rapamycin-sensitive phosphorylation of PKC on a carboxy-terminal site by an atypical PKC complex
    W H Ziegler
    Protein Phosphorylation Laboratory, Imperial Cancer Research Fund, 44 Lincoln s Inn Fields, London, WC2A 3PX, UK
    Curr Biol 9:522-9. 1999
    ..Although this site is crucial to the control of this class of enzymes, the upstream kinase(s) has not been identified...
  3. pmc Sac phosphatase domain proteins
    W E Hughes
    Protein Phosphorylation Laboratory, Imperial Cancer Research Fund, 44, Lincoln s Inn Fields, London WC2A 3PX, UK
    Biochem J 350:337-52. 2000
    ..This review describes the Sac phosphatase domain-containing proteins and their actions, with particular reference to the genetic and biochemical insights provided by study of the yeast Saccharomyces cerevisiae...
  4. ncbi request reprint Rho GTPase control of protein kinase C-related protein kinase activation by 3-phosphoinositide-dependent protein kinase
    P Flynn
    Imperial Cancer Research Fund, Protein Phosphorylation Laboratory, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
    J Biol Chem 275:11064-70. 2000
    ..Furthermore, this in vivo complex is maintained after phosphoinositide 3-kinase inhibition. The control of PRKs by PDK1 thus evidences a novel strategy of substrate-directed control involving GTPases...
  5. ncbi request reprint Imaging protein kinase Calpha activation in cells
    T Ng
    Protein Phosphorylation Laboratory and Cell Biophysics Laboratory, Imperial Cancer Research Fund ICRF, 44 Lincoln s Inn Fields, London, WC2A 3PX, UK
    Science 283:2085-9. 1999
    ..This approach enabled the imaging of PKCalpha activation in live and fixed cultured cells and was also applied to pathological samples...
  6. ncbi request reprint p42 MAPK phosphorylates 80 kDa MARCKS at Ser-113
    D C Schönwasser
    Protein Phosphorylation Laboratory, Imperial Cancer Research Fund, London, UK
    FEBS Lett 395:1-5. 1996
    ..1994) J. Biol. Chem. 269, 18299-18302], its phosphorylation is not subject to acute regulation by p42 MAPK in Swiss 3T3 cells...
  7. ncbi request reprint SAC1 encodes a regulated lipid phosphoinositide phosphatase, defects in which can be suppressed by the homologous Inp52p and Inp53p phosphatases
    W E Hughes
    Protein Phosphorylation, Imperial Cancer Research Fund, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
    J Biol Chem 275:801-8. 2000
    ..It is concluded that SAC1 encodes a novel lipid phosphoinositide phosphatase in which specific mutations can cause the sac1 phenotypes by altering the in vivo regulation of the protein rather than by destroying phosphatase activity...
  8. ncbi request reprint Protein kinase C isotypes controlled by phosphoinositide 3-kinase through the protein kinase PDK1
    J A Le Good
    Protein Phosphorylation Laboratory, Imperial Cancer Research Fund, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Science 281:2042-5. 1998
    ..The activation loop phosphorylation of PKCdelta in response to serum stimulation of cells was PI 3-kinase-dependent and was enhanced by PDK1 coexpression...
  9. ncbi request reprint Regulated binding of the protein kinase C substrate GAP-43 to the V0/C2 region of protein kinase C-delta
    L V Dekker
    Protein Phosphorylation Laboratory, Imperial Cancer Research Fund, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
    J Biol Chem 272:12747-53. 1997
    ..It is concluded that protein kinase C-delta interacts with GAP-43 through the V0/C2-like domain, outside the catalytic site, and that this interaction is modulated by intracellular Ca2+...
  10. ncbi request reprint PRK1 is targeted to endosomes by the small GTPase, RhoB
    H Mellor
    Protein Phosphorylation Laboratory, Imperial Cancer Research Fund, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
    J Biol Chem 273:4811-4. 1998
    ..Translocation of PRK1 to the endosomal compartment by RhoB is accompanied by a shift in the electrophoretic mobility of the kinase indicative of an accompanying activation...
  11. pmc The broad specificity of dominant inhibitory protein kinase C mutants infers a common step in phosphorylation
    P Garcia-Paramio
    Imperial Cancer Research Fund, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Biochem J 333:631-6. 1998
    ..In the case of the PKCalpha mutant, it was shown that inhibition required the full-length mutant protein. The results provide evidence for the involvement of a common step in the phosphorylation of all PKC isotypes...
  12. ncbi request reprint Multiple interactions of PRK1 with RhoA. Functional assignment of the Hr1 repeat motif
    P Flynn
    Protein Phosphorylation Laboratory, Imperial Cancer Research Fund, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
    J Biol Chem 273:2698-705. 1998
    ..Additionally, it is observed that the V14RhoA mutant binds HR1a but does not bind HR1b. This distinct binding behavior corroborates the conclusion that there are independent contacts on RhoA for the HR1aPRK1 and HR1bPRK1 motifs...
  13. ncbi request reprint c-Met signalling: spatio-temporal decisions
    S Kermorgant
    Protein Phosphorylation Laboratory, Cancer Research UK London Research Institute, London, UK
    Cell Cycle 4:352-5. 2005
    ..The mechanisms involved and the implications for signalling studies are discussed...
  14. ncbi request reprint Crystal structure of the C2 domain from protein kinase C-delta
    H Pappa
    Structural Biology, Imperial Cancer Research Fund, London, UK
    Structure 6:885-94. 1998
    ..Biochemical data suggest that this domain serves to translocate novel PKC family members to the plasma membrane and may influence binding of PKC activators...
  15. ncbi request reprint Identification of multiple, novel, protein kinase C-related gene products
    R H Palmer
    Protein Phosphorylation Laboratory, Imperial Cancer Research Fund, London, UK
    FEBS Lett 356:5-8. 1994
    ..The origin and relationships of these predicted proteins is discussed...
  16. pmc Compartmental signal modulation: Endosomal phosphatidylinositol 3-phosphate controls endosome morphology and selective cargo sorting
    N Fili
    Cell Biophysics Laboratory and Protein Phosphorylation Laboratory, London Research Institute, Cancer Research UK, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
    Proc Natl Acad Sci U S A 103:15473-8. 2006
    ..The ability to acutely and selectively influence compartmental behavior as exemplified here for endomsomes clearly illustrates the power of the approach used to dissect the role of localized signals and events...
  17. ncbi request reprint Protein kinases, from B to C
    A J Cameron
    Protein Phosphorylation Lab, London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, U K
    Biochem Soc Trans 35:1013-7. 2007
    ..It is concluded that the distinct behaviours of PKB and PKC proteins are defined by the typical ground states of these proteins...
  18. ncbi request reprint The stress-activated phosphatidylinositol 3-phosphate 5-kinase Fab1p is essential for vacuole function in S. cerevisiae
    F T Cooke
    Protein Phosphorylation Laboratory, Imperial Cancer Research Fund, London, UK
    Curr Biol 8:1219-22. 1998
    ..Deletion of the FAB1 gene produces a loss of vacuolar morphology [6]; it is therefore concluded that PI(3,5)P2, the lipid product of Fab1p, is required for normal vacuolar function...
  19. ncbi request reprint Rho-dependence of Schizosaccharomyces pombe Pck2
    L G Sayers
    Protein Phosphorylation Laboratory, Imperial Cancer Research Fund, London, UK
    Genes Cells 5:17-27. 2000
    ..The structural similarity between the Schizosaccaromyces pombe Pck proteins and the mammalian Rho-dependent protein kinase C-related family, has led us to investigate the relationship between the function of Rho and that of Pck1/2...
  20. pmc Expression of mammalian protein kinase C in Schizosaccharomyces pombe: isotype-specific induction of growth arrest, vesicle formation, and endocytosis
    N T Goode
    Protein Phosphorylation Laboratory, Imperial Cancer Research Fund, London, United Kingdom
    Mol Biol Cell 5:907-20. 1994
    ..Thus expression of specific mammalian PKC isotypes up-regulates endocytosis in S. pombe, providing a likely explanation for PKC-mediated receptor internalization in higher eukaryotes...
  21. ncbi request reprint PRK1 phosphorylates MARCKS at the PKC sites: serine 152, serine 156 and serine 163
    R H Palmer
    Protein Phosphorylation Laboratory, Imperial Cancer Research Fund, London, UK
    FEBS Lett 378:281-5. 1996
    ..The implications for MARCKS as a marker of PKC activation and as a point of signal convergence are discussed...
  22. ncbi request reprint Phosphorylation of protein kinase C-alpha on serine 657 controls the accumulation of active enzyme and contributes to its phosphatase-resistant state
    F Bornancin
    Imperial Cancer Research Fund, Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
    J Biol Chem 272:3544-9. 1997
    ..These results are discussed in the context of a working model of PKCalpha behavior, providing insight into the workings of other kinases with equivalent sites of phosphorylation...
  23. ncbi request reprint Identification and characterisation of a novel splice variant of synaptojanin1
    R Woscholski
    Protein Phosphorylation Laboratory, Imperial Cancer Research Fund, London, UK
    FEBS Lett 432:5-8. 1998
    ..However, the deletion of the SAC1 domain does not alter PtdInsP3 5-phosphatase activity demonstrating that the SAC1 domain is not necessary for catalytic function...
  24. pmc Endosomal localization of phospholipase D 1a and 1b is defined by the C-termini of the proteins, and is independent of activity
    W E Hughes
    Protein Phosphorylation Laboratory, Imperial Cancer Research Fund, 44, Lincoln s Inn Fields, London WC2A 3PX, UK
    Biochem J 356:727-36. 2001
    ....
  25. ncbi request reprint Integrin-protein kinase C relationships
    J Ivaska
    Cancer Research UK, London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Biochem Soc Trans 31:90-3. 2003
    ..In the present paper, the developing understanding of the bi-directional relationship between the protein kinase C family of signal transducers and integrins is discussed...
  26. ncbi request reprint Biochemical characterization of the free catalytic p110 alpha and the complexed heterodimeric p110 alpha.p85 alpha forms of the mammalian phosphatidylinositol 3-kinase
    R Woscholski
    Protein Phosphorylation Laboratory, Imperial Cancer Research Fund, London, United Kingdom
    J Biol Chem 269:25067-72. 1994
    ..p110 alpha) caused this effect. This mode of regulation is discussed in the context of lipid kinase activation in vivo...
  27. ncbi request reprint The ubiquitous phosphoinositides
    P J Parker
    Protein Phosphorylation Laboratory, London Research Institute CRUK, 44 Lincoln s Inn Fields, London EC2A 3PX, UK
    Biochem Soc Trans 32:893-8. 2004
    ..The metabolic map of this pathway and the nature of the binding partner interactions are reviewed...
  28. ncbi request reprint Cloning and expression patterns of two members of a novel protein-kinase-C-related kinase family
    R H Palmer
    Protein Phosphorylation Laboratory, Imperial Cancer Research Fund, London, England
    Eur J Biochem 227:344-51. 1995
    ..PRK1 and PRK2, as well as a third member of this family, PRK3, show distinct patterns of expression in adult tissues...
  29. pmc Conjugation of monoclonal antibodies to a synthetic peptide substrate for protein kinase: a method for labelling antibodies with 32P
    B M Foxwell
    Department of Cellular Pharmacology, Imperial Cancer Research Fund, London, UK
    Br J Cancer 57:489-93. 1988
    ..The application of this phosphorylation technique should allow the therapeutic potential of targeted 32P to be assessed...
  30. ncbi request reprint Regulation of epidermal growth factor receptor traffic by the small GTPase rhoB
    A Gampel
    Department of Biochemistry, School of Medical Sciences University of Bristol, Bristol, BS8 1TD, UK
    Curr Biol 9:955-8. 1999
    ..We have recently demonstrated that RhoB binds the RhoA effector, PRK1 and targets it to the endosomal compartment [7]. We show here that RhoB acts through PRK1 to regulate the kinetics of epidermal growth factor receptor traffic...
  31. pmc Ezrin is a downstream effector of trafficking PKC-integrin complexes involved in the control of cell motility
    T Ng
    Richard Dimbleby Department of Cancer Research, St Thomas Hospital, Lambeth Palace Road, London SE1 7EH, UK
    EMBO J 20:2723-41. 2001
    ..We provide the first evidence that PKCalpha or a PKCalpha-associated serine/threonine kinase can phosphorylate the ERM C-terminal threonine residue within a kinase-ezrin molecular complex in vivo...
  32. pmc Spatially selective sampling of single cells using optically trapped fusogenic emulsion droplets: a new single-cell proteomic tool
    Peter M P Lanigan
    The Single Cell Proteomics Group, Chemical Biology Centre CBC, Imperial College London, Exhibition Road, London SW7 2AZ, UK
    J R Soc Interface 5:S161-8. 2008
    ..Material transfer from the cell to the SDM across the membrane tether was monitored by tracking membrane-localized enhanced green fluorescent protein...
  33. ncbi request reprint The myristoylated alanine-rich C-kinase substrate (MARCKS) is sequentially phosphorylated by conventional, novel and atypical isotypes of protein kinase C
    T Herget
    Institute of Physiological Chemistry, University of Mainz, Germany
    Eur J Biochem 233:448-57. 1995
    ..There was no dramatic difference in the sequential phosphorylation of MARCKS detectable when comparing the four PKC isotypes. The results are discussed in the context of the functional significance of MARCKS phosphorylation...
  34. pmc Hormonal regulation of Gi2 alpha-subunit phosphorylation in intact hepatocytes
    M Bushfield
    Department of Biochemistry, University of Glasgow, Scotland, U K
    Biochem J 268:449-57. 1990
    ..We suggest that there are two possible sites for the phosphorylation of alpha-Gi2; one for C-kinase and the other for an unidentified kinase whose action is triggered by A-kinase activation...
  35. ncbi request reprint Differential regulation of glycogen synthase kinase-3 beta by protein kinase C isotypes
    N Goode
    Imperial Cancer Research Fund, Lincoln s Inn Fields, London, United Kingdom
    J Biol Chem 267:16878-82. 1992
    ..Phosphorylation of GSK-3 beta by PKC results in its specific inactivation. These results are consistent with a model in which activation of PKC stimulates c-Jun DNA binding by inhibiting its phosphorylation by GSK-3 beta...
  36. ncbi request reprint Identification of the phosphorylated region responsible for the permissive activation of protein kinase C
    S M Cazaubon
    Imperial Cancer Research Fund, London, United Kingdom
    J Biol Chem 268:17559-63. 1993
    ..Coexpression of this PKC alpha mutant and wild type PKC beta demonstrates that the mutant has a dominant effect upon PKC beta phosphorylation. The location of this region and its phosphorylation in relation to PKC function are discussed...
  37. pmc Phosphorylation of GAP-43 (growth-associated protein of 43 kDa) by conventional, novel and atypical isotypes of the protein kinase C gene family: differences between oligopeptide and polypeptide phosphorylation
    S A Oehrlein
    Institute of Physiological Chemistry, Johannes Gutenberg University, Mainz, Germany
    Biochem J 317:219-24. 1996
    ..These results suggest that there are multiple sites of interaction between GAP-43 and PKC...
  38. ncbi request reprint Gab1 phosphorylation: a novel mechanism for negative regulation of HGF receptor signaling
    P Gual
    Institute for Cancer Research and Treatment (IRCC, University of Torino Medical School, Str. Prov. 142, Km 3.95, 10060 Candiolo, Italy
    Oncogene 20:156-66. 2001
    ..These data provide a novel negative mechanism for the HGF receptor signaling pathways and highlight a potentially useful target for inhibitors of invasive growth...
  39. ncbi request reprint Expression and characterization of protein kinase C-delta
    A R Olivier
    Protein Phosphorylation Laboratory, Imperial Cancer Research Fund, London, England
    Eur J Biochem 200:805-10. 1991
    ..Like PKC-epsilon, PKC-delta displays no Ca2+ dependence for activation. The substrate specificity of PCK-delta is similar to that of PKC-epsilon but quite different from other PKCs...
  40. ncbi request reprint Biochemical properties of rat protein kinase C-eta expressed in COS cells
    L V Dekker
    Sandoz Institute for Medical Research, London, UK
    FEBS Lett 312:195-9. 1992
    ..Various PKC pseudosubstrate peptides are phosphorylated by PKC-eta in a phospholipid and TPA-dependent but calcium-independent manner. The polypeptide histone IIIS is a poor substrate...
  41. pmc Protein kinase Cepsilon is required for macrophage activation and defense against bacterial infection
    A Castrillo
    Instituto de Bioquímica Centro Mixto Consejo Superior de Investigaciones Cientificas UCM, Facultad de Farmacia, Universidad Complutense, 28040 Madrid, Spain
    J Exp Med 194:1231-42. 2001
    ..Furthermore, we demonstrate that in the absence of PKCepsilon, host defense against bacterial infection is severely compromised, resulting in an increased incidence of mortality...
  42. ncbi request reprint Unique substrate specificity and regulatory properties of PKC-epsilon: a rationale for diversity
    D Schaap
    Ludwig Institute for Cancer Research, London, England
    FEBS Lett 243:351-7. 1989
    ..Subsequent analysis demonstrated that PKC-epsilon, while showing certain properties characteristic of the PKC family, has a quite distinct substrate specificity and is independent of Ca2+...
  43. pmc Interferon alpha induces protein kinase C-epsilon (PKC-epsilon) gene expression and a 4.7-kb PKC-epsilon-related transcript
    C Wang
    Department of Pathology, University of Tennessee Health Science Center, Memphis 38163
    Proc Natl Acad Sci U S A 90:6944-8. 1993
    ....
  44. ncbi request reprint The activation of phosphatidylinositol 3-kinase by Ras
    T Kodaki
    Protein Phosphorylation Laboratory, Imperial Cancer Research Fund, London, UK
    Curr Biol 4:798-806. 1994
    ..Recent evidence indicates that Ras can bind to the p85 alpha/p110 alpha complex. We describe here the functional regulation of the mammalian phosphatidylinositol 3-kinase complex by Ras...
  45. ncbi request reprint Specific proteolysis of the kinase protein kinase C-related kinase 2 by caspase-3 during apoptosis. Identification by a novel, small pool expression cloning strategy
    V L Cryns
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 272:29449-53. 1997
    ..Both of the major apoptotic cleavage sites of PRK2 in vivo lie within its regulatory domain, suggesting that its activity may be deregulated by proteolysis...
  46. ncbi request reprint Multiple, distinct forms of bovine and human protein kinase C suggest diversity in cellular signaling pathways
    L Coussens
    Science 233:859-66. 1986
    ..The corresponding genes are located on distinct human chromosomes; the possibility of even greater genetic complexity of this gene family is suggested by Northern and Southern hybridization analyses...
  47. ncbi request reprint The complete primary structure of protein kinase C--the major phorbol ester receptor
    P J Parker
    Science 233:853-9. 1986
    ....
  48. ncbi request reprint Alternative splicing increases the diversity of the human protein kinase C family
    L Coussens
    Department of Developmental Biology, Genentech, Inc, South San Francisco, CA 94080
    DNA 6:389-94. 1987
    ..PKC beta 1 and beta 2 mRNAs are expressed in a selective manner in both human hematopoietic cells and bovine brain tissues...
  49. ncbi request reprint Protein kinases in cellular signal transduction: tyrosine kinase growth factor receptors and protein kinase C
    A Ullrich
    Cold Spring Harb Symp Quant Biol 51:713-24. 1986
  50. ncbi request reprint The human T3 gamma chain is phosphorylated at serine 126 in response to T lymphocyte activation
    A A Davies
    J Biol Chem 262:10918-21. 1987
    ..Immune activation of T cells via the protein kinase C pathway thus induces phosphorylation of a single site on the T3 gamma chain, namely serine 126...
  51. ncbi request reprint Up-regulation of protein kinase C-epsilon promotes the expression of cytokine-inducible nitric oxide synthase in RAW 264.7 cells
    M J Diaz-Guerra
    Instituto de Bioquímica Consejo Superior de Investigaciones Cientifícas, Facultad de Farmacia, Universidad Complutense, 28040 Madrid, Spain
    J Biol Chem 271:32028-33. 1996
    ..These results suggest that PKC-epsilon can regulate a pathway that promotes iNOS expression in macrophages in response to phorbol ester activation...