Dylan M Owen
Affiliation: Imperial College
- Optical techniques for imaging membrane lipid microdomains in living cellsDylan M Owen
Chemical Biology Centre, Imperial College London, London, UK
Semin Cell Dev Biol 18:591-8. 2007..We also look at novel emerging instruments and methods that promise to overcome our current technological limitations and shed new light on these important structures...
- Excitation-resolved hyperspectral fluorescence lifetime imaging using a UV-extended supercontinuum sourceDylan M Owen
Chemical Biology Centre, Photonics Group, Department of Physics, Imperial College London, UK
Opt Lett 32:3408-10. 2007..The system is capable of resolving the excitation spectrum, emission spectrum, and fluorescence decays in an optically sectioned image...
- High plasma membrane lipid order imaged at the immunological synapse periphery in live T cellsDylan M Owen
Chemical Biology Centre, Imperial College, London, UK
Mol Membr Biol 27:178-89. 2010..The observed spatial patterning of membrane order in the immunological synapse depends on active receptor signalling...
- A compact, multidimensional spectrofluorometer exploiting supercontinuum generationHugh B Manning
Chemical Biology Centre, Department of Chemistry, Imperial College London, London, UK
J Biophotonics 1:494-505. 2008..It can be applied to in situ measurements via a fibre-optic probe in medical and other contexts and is demonstrated here to provide a comprehensive characterisation of tissue autofluorescence...
- High speed unsupervised fluorescence lifetime imaging confocal multiwell plate reader for high content analysisClifford B Talbot
Photonics Group, Imperial College London, London SW72AZ, UK
J Biophotonics 1:514-21. 2008..We demonstrate its applications to test dye solutions, fixed and live cells and FLIM-FRET...
- Three-dimensional molecular mapping in a microfluidic mixing device using fluorescence lifetime imagingTom Robinson
Chemical Biology Centre, Department of Chemical Engineering, Imperial College London, London, UK
Opt Lett 33:1887-9. 2008..Following experimental and CFD optimization, mixing times down to 1.3+/-0.4 ms were achieved with the single-layer microfluidic device...
- Lipid order and molecular assemblies in the plasma membrane of eukaryotic cellsMarek Cebecauer
National Heart and Lung Institute, Imperial College London, London, UK
Biochem Soc Trans 37:1056-60. 2009..The role of lipids and their physical properties in helping to control the existence of these structures is discussed. Technological improvements for live cell imaging of membrane components are also reviewed...
- Rapid hyperspectral fluorescence lifetime imagingPieter De Beule
Department of Physics, Imperial College London, South Kensington Campus, London, SW7 2AZ, United Kingdom
Microsc Res Tech 70:481-4. 2007..We demonstrate the acquisition of whole-field optically sectioned hyperspectral fluorescence lifetime image stacks (with 32 spectral bins) in less than 40 s and illustrate its application to unstained biological tissue...
- Dynamics of subsynaptic vesicles and surface microclusters at the immunological synapseMarco A Purbhoo
Section of Hepatology and Gastroenterology, Department of Medicine, Medical School Building, Imperial College London, London W2 1PG, UK
Sci Signal 3:ra36. 2010..Together, these data show that the movement of LAT-containing vesicles is linked to the organization of protein microclusters and suggest an important role for vesicular LAT in the SLP-76 signalosome...
- Improved sectioning in a slit scanning confocal microscopeVincent Poher
Photonics Group, Blackett Laboratory, Department of Chemistry, Imperial College London, London, UK
Opt Lett 33:1813-5. 2008..2 times better than the slit confocal resolution, and out-of-focus image suppression is calculated to attenuate with defocus faster by 1 order of magnitude than in the point confocal case...
- Fluorescence lifetime imaging provides enhanced contrast when imaging the phase-sensitive dye di-4-ANEPPDHQ in model membranes and live cellsDylan M Owen
Chemical Biology Centre, Department of Physics, Imperial College London, London, United Kingdom
Biophys J 90:L80-2. 2006..Detection of cholesterol-rich membrane microdomains is confirmed by observation of the temperature dependence of membrane order and by cholesterol depletion using methyl-beta-cyclodextrin...
- High-speed high-resolution imaging of intercellular immune synapses using optical tweezersStephane Oddos
Chemical Biology Centre, Imperial College London, United Kingdom
Biophys J 95:L66-8. 2008....
- Superresolution microscopy reveals nanometer-scale reorganization of inhibitory natural killer cell receptors upon activation of NKG2DSophie V Pageon
Division of Cell and Molecular Biology, Sir Alexander Fleming Building, Imperial College London, London SW7 2AZ, UK
Sci Signal 6:ra62. 2013....
- The ternary Rab27a-Myrip-Myosin VIIa complex regulates melanosome motility in the retinal pigment epitheliumVanda S Lopes
Molecular and Cellular Medicine, Faculty of Medicine, Imperial College London, London SW7 2AZ, UK
Traffic 8:486-99. 2007....