Anthony I Magee

Summary

Affiliation: Imperial College
Country: UK

Publications

  1. pmc Attenuation of hedgehog acyltransferase-catalyzed sonic Hedgehog palmitoylation causes reduced signaling, proliferation and invasiveness of human carcinoma cells
    Antonios D Konitsiotis
    Molecular Medicine Section, National Heart and Lung Institute, Imperial College London, London, United Kingdom
    PLoS ONE 9:e89899. 2014
  2. ncbi request reprint Fatty acylation and prenylation of proteins: what's hot in fat
    Tony Magee
    Imperial College London, Section of Cell and Molecular Biology, Division of Biomedical Sciences, Sir Alexander Fleming Building, London, SW72AZ, UK
    Curr Opin Cell Biol 17:190-6. 2005
  3. pmc Detergent-resistant membranes and the protein composition of lipid rafts
    Anthony I Magee
    Division of Biomedical Sciences, Imperial College Faculty of Medicine, SAF Building, Exhibition Road, South Kensington, London SW7 2AZ, UK
    Genome Biol 4:234. 2003
  4. pmc Are prenyl groups on proteins sticky fingers or greasy handles?
    Anthony I Magee
    Section of Cell and Molecular Biology, Division of Biomedical Sciences, Faculty of Medicine, Imperial College London, Exhibition Road, South Kensington, London SW7 2AZ, UK
    Biochem J 376:e3-4. 2003
  5. pmc High plasma membrane lipid order imaged at the immunological synapse periphery in live T cells
    Dylan M Owen
    Chemical Biology Centre, Imperial College, London, UK
    Mol Membr Biol 27:178-89. 2010
  6. ncbi request reprint Control of immune responses by trafficking cell surface proteins, vesicles and lipid rafts to and from the immunological synapse
    Sabrina B Taner
    Department of Biological Sciences, Imperial College London, SW7 2AZ, UK
    Traffic 5:651-61. 2004
  7. doi request reprint DHHC2 is a protein S-acyltransferase for Lck
    Ruth Zeidman
    Molecular Medicine Section, National Heart and Lung Institute, Imperial College London, Sir Alexander Fleming Building, South Kensington, London, UK
    Mol Membr Biol 28:473-86. 2011
  8. doi request reprint A compact, multidimensional spectrofluorometer exploiting supercontinuum generation
    Hugh B Manning
    Chemical Biology Centre, Department of Chemistry, Imperial College London, London, UK
    J Biophotonics 1:494-505. 2008
  9. ncbi request reprint Cold-induced coalescence of T-cell plasma membrane microdomains activates signalling pathways
    Anthony I Magee
    Division of Biomedical Sciences, Imperial College Faculty of Medicine, Exhibition Road, South Kensington, London, SW7 2AZ, UK
    J Cell Sci 118:3141-51. 2005
  10. ncbi request reprint Optical techniques for imaging membrane lipid microdomains in living cells
    Dylan M Owen
    Chemical Biology Centre, Imperial College London, London, UK
    Semin Cell Dev Biol 18:591-8. 2007

Collaborators

  • Dylan M Owen
  • Daniel M Davis
  • Ingela Parmryd
  • Miguel C Seabra
  • John R Couchman
  • N Jan Chalupny
  • David M Grant
  • Marek Cebecauer
  • L Al-Gazali
  • Hugh B Manning
  • David W Holden
  • Yoshifumi Itoh
  • M L Dustin
  • Sunil Kumar
  • Thomas Schmidt
  • Bassam R Ali
  • Ruth Zeidman
  • Shu Chun Chang
  • Tony Magee
  • Antonios D Konitsiotis
  • Edward W Tate
  • Biljana Jovanovic
  • Sabrina B Taner
  • William P Heal
  • Caroline S Jackson
  • Nadine S Lossi
  • Ivan Plaza-Menacho
  • Ka Fai Leung
  • Piet H M Lommerse
  • Niina J Pirinen
  • Anna T Reinicke
  • Marc Veldhoen
  • Fiona E McCann
  • Konstantina Eleme
  • Björn Önfelt
  • Ibrahim Y Hawash
  • Shabih e Hassnain Syed
  • Naoko Masumoto
  • Paulina Ciepla
  • Christopher P Palmer
  • Sara Bessin
  • Gemma Buckland
  • Philipp Eissmann
  • Megan H Wright
  • Barbara Mulloy
  • Cliona Boyle
  • Nathalie Rolhion
  • Bart J L Eggen
  • Oliver Gimm
  • Rudi Baron
  • Robert M W Hofstra
  • Tineke van der Sluis
  • Charles H C M Buys
  • Clare M Isacke
  • Harry Hollema
  • Herman P Spaink
  • Karin Vastenhoud
  • James L Hutchinson
  • Mario N Penha-Goncalves
  • Piero Mastroeni
  • Brigitta Stockinger
  • John Trowsdale
  • Adrian P Kelly
  • Lucy M Collinson
  • Colin Hopkins
  • David Cosman
  • Niina Pirinen
  • Kamala P Kesavan
  • Brian Trinnaman
  • Stephen Martin
  • Jeremy Adler
  • Stamatis N Pagakis
  • Robert L Geahlen
  • Jon Hutchinson
  • Marietta L Harrison
  • Sarah Major

Detail Information

Publications29

  1. pmc Attenuation of hedgehog acyltransferase-catalyzed sonic Hedgehog palmitoylation causes reduced signaling, proliferation and invasiveness of human carcinoma cells
    Antonios D Konitsiotis
    Molecular Medicine Section, National Heart and Lung Institute, Imperial College London, London, United Kingdom
    PLoS ONE 9:e89899. 2014
    ..Our data identify Hhat as a key player in Hh-dependent signaling and tumour cell transformed behaviour. ..
  2. ncbi request reprint Fatty acylation and prenylation of proteins: what's hot in fat
    Tony Magee
    Imperial College London, Section of Cell and Molecular Biology, Division of Biomedical Sciences, Sir Alexander Fleming Building, London, SW72AZ, UK
    Curr Opin Cell Biol 17:190-6. 2005
    ..The two types of prenyl group, geranylgeranyl and farnesyl, themselves have surprisingly specific targeting roles for Ras superfamily members...
  3. pmc Detergent-resistant membranes and the protein composition of lipid rafts
    Anthony I Magee
    Division of Biomedical Sciences, Imperial College Faculty of Medicine, SAF Building, Exhibition Road, South Kensington, London SW7 2AZ, UK
    Genome Biol 4:234. 2003
    ..Several recent proteomic studies have addressed the composition of lipid rafts, but the different definitions used for lipid rafts need scrutinizing before results can be evaluated...
  4. pmc Are prenyl groups on proteins sticky fingers or greasy handles?
    Anthony I Magee
    Section of Cell and Molecular Biology, Division of Biomedical Sciences, Faculty of Medicine, Imperial College London, Exhibition Road, South Kensington, London SW7 2AZ, UK
    Biochem J 376:e3-4. 2003
    ....
  5. pmc High plasma membrane lipid order imaged at the immunological synapse periphery in live T cells
    Dylan M Owen
    Chemical Biology Centre, Imperial College, London, UK
    Mol Membr Biol 27:178-89. 2010
    ..The observed spatial patterning of membrane order in the immunological synapse depends on active receptor signalling...
  6. ncbi request reprint Control of immune responses by trafficking cell surface proteins, vesicles and lipid rafts to and from the immunological synapse
    Sabrina B Taner
    Department of Biological Sciences, Imperial College London, SW7 2AZ, UK
    Traffic 5:651-61. 2004
    ....
  7. doi request reprint DHHC2 is a protein S-acyltransferase for Lck
    Ruth Zeidman
    Molecular Medicine Section, National Heart and Lung Institute, Imperial College London, Sir Alexander Fleming Building, South Kensington, London, UK
    Mol Membr Biol 28:473-86. 2011
    ..DHHC2 localizes primarily to the endoplasmic reticulum and Golgi apparatus suggesting that it is involved in S-acylation of newly-synthesized or recycling Lck involved in T cell signalling...
  8. doi request reprint A compact, multidimensional spectrofluorometer exploiting supercontinuum generation
    Hugh B Manning
    Chemical Biology Centre, Department of Chemistry, Imperial College London, London, UK
    J Biophotonics 1:494-505. 2008
    ..It can be applied to in situ measurements via a fibre-optic probe in medical and other contexts and is demonstrated here to provide a comprehensive characterisation of tissue autofluorescence...
  9. ncbi request reprint Cold-induced coalescence of T-cell plasma membrane microdomains activates signalling pathways
    Anthony I Magee
    Division of Biomedical Sciences, Imperial College Faculty of Medicine, Exhibition Road, South Kensington, London, SW7 2AZ, UK
    J Cell Sci 118:3141-51. 2005
    ..These results support the concept that lipid rafts can form in membranes of live cells and that their coalescence stimulates signalling...
  10. ncbi request reprint Optical techniques for imaging membrane lipid microdomains in living cells
    Dylan M Owen
    Chemical Biology Centre, Imperial College London, London, UK
    Semin Cell Dev Biol 18:591-8. 2007
    ..We also look at novel emerging instruments and methods that promise to overcome our current technological limitations and shed new light on these important structures...
  11. doi request reprint Lipid order and molecular assemblies in the plasma membrane of eukaryotic cells
    Marek Cebecauer
    National Heart and Lung Institute, Imperial College London, London, UK
    Biochem Soc Trans 37:1056-60. 2009
    ..The role of lipids and their physical properties in helping to control the existence of these structures is discussed. Technological improvements for live cell imaging of membrane components are also reviewed...
  12. doi request reprint Analysis of protein acylation
    Ruth Zeidman
    Molecular Medicine, National Heart and Lung Institute, Imperial College London, London, United Kingdom
    Curr Protoc Protein Sci . 2009
    ....
  13. doi request reprint Acyltransferases for secreted signalling proteins (Review)
    Shu Chun Chang
    Section of Molecular Medicine, National Heart and Lung Institute, Imperial College London, London, UK
    Mol Membr Biol 26:104-13. 2009
    ..Characterization of these enzymes could lead to their identification as therapeutic targets for diverse human diseases such as cancers, obesity and diabetes...
  14. pmc Cell surface organization of stress-inducible proteins ULBP and MICA that stimulate human NK cells and T cells via NKG2D
    Konstantina Eleme
    Department of Biological Sciences, Imperial College, London SW7 2AZ, UK
    J Exp Med 199:1005-10. 2004
    ..Finally, expression of a truncated form of MICA that lacks the putative site for S-acylation and the cytoplasmic tail can be expressed at the cell surface, but is unable to activate NK cells...
  15. ncbi request reprint Rab GTPases containing a CAAX motif are processed post-geranylgeranylation by proteolysis and methylation
    Ka Fai Leung
    Molecular and Cellular Medicine Section, National Heart and Lung Institute, Imperial College London, London SW7 2AZ, United Kingdom
    J Biol Chem 282:1487-97. 2007
    ..Our findings also imply that those Rabs that undergo post-prenylation processing follow an indirect targeting pathway requiring initial endoplasmic reticulum membrane association prior to specific organelle targeting...
  16. doi request reprint Signalling complexes and clusters: functional advantages and methodological hurdles
    Marek Cebecauer
    Section of Molecular Medicine, National Heart and Lung Institute, Imperial College London, London SW7 2AZ, UK
    J Cell Sci 123:309-20. 2010
    ....
  17. doi request reprint Bioorthogonal chemical tagging of protein cholesterylation in living cells
    William P Heal
    Department of Chemistry, Imperial College London, Exhibition Rd, London SW7 2AZ, UK
    Chem Commun (Camb) 47:4081-3. 2011
    ..This enables rapid multiplexed fluorescence detection and affinity labelling of protein cholesterylation, as exemplified by Sonic hedgehog protein, opening up new approaches for the de novo identification of cholesterylated proteins...
  18. ncbi request reprint Imaging metabolism of phosphatidylinositol 4,5-bisphosphate in T-cell GM1-enriched domains containing Ras proteins
    Ingela Parmryd
    Division of Membrane Biology, National Institute for Medical Research, The Ridgeway, Mill Hill, London, NW7 1AA, UK
    Exp Cell Res 285:27-38. 2003
    ..The results demonstrate that T-cell PI(4,5)P(2) metabolism is occurring in GM1-enriched domains and that Ras proteins are present in these domains in vivo...
  19. pmc Two distinct sites in sonic Hedgehog combine for heparan sulfate interactions and cell signaling functions
    Shu Chun Chang
    Molecular Medicine Section, National Heart and Lung Institute, Imperial College London, South Kensington, London SW7 2AZ, United Kingdom
    J Biol Chem 286:44391-402. 2011
    ..These studies provide a new insight into the functional roles of hShh interactions with HSPGs, which may allow targeting this aspect of hShh biology in, for example, pancreatic ductal adenocarcinoma...
  20. ncbi request reprint Lipid rafts: cell surface platforms for T cell signaling
    Tony Magee
    Imperial College Faculty of Medicine, London, SW7 2AZ, UK
    Biol Res 35:127-31. 2002
    ..Thus rafts are hotspots for both protein and lipid signalling pathways...
  21. doi request reprint Protein acyl thioesterases (Review)
    Ruth Zeidman
    Molecular Medicine, National Heart and Lung Institute, Sir Alexander Fleming Building, Imperial College London, London, UK
    Mol Membr Biol 26:32-41. 2009
    ..PPT1, on the other hand, is involved in the lysosomal degradation of S-acylated proteins and PPT1 deficiency causes the disease infant neuronal ceroid lipofuscinosis...
  22. pmc Fluorescence lifetime imaging provides enhanced contrast when imaging the phase-sensitive dye di-4-ANEPPDHQ in model membranes and live cells
    Dylan M Owen
    Chemical Biology Centre, Department of Physics, Imperial College London, London, United Kingdom
    Biophys J 90:L80-2. 2006
    ..Detection of cholesterol-rich membrane microdomains is confirmed by observation of the temperature dependence of membrane order and by cholesterol depletion using methyl-beta-cyclodextrin...
  23. pmc The Salmonella SPI-2 effector SseJ exhibits eukaryotic activator-dependent phospholipase A and glycerophospholipid : cholesterol acyltransferase activity
    Nadine S Lossi
    Centre for Molecular Microbiology and Infection, Imperial College London, Armstrong Road, London SW7 2AZ, UK
    Microbiology 154:2680-8. 2008
    ..Analysis of the activating factor(s) shows that it is soluble and heat- and protease-sensitive. We conclude that PLA and GCAT activities of SseJ are potentiated by proteinaceous eukaryotic factor(s)...
  24. ncbi request reprint Transduction of naive CD4 T cells with kinase-deficient Lck-HIV-Tat fusion protein dampens T cell activation and provokes a switch to regulatory function
    Marc Veldhoen
    Division of Molecular Immunology, The National Institute for Medical Research, London, UK
    Eur J Immunol 35:207-16. 2005
    ..Our data indicate that signals encountered by a naive T cell during its initial activation can profoundly influence its subsequent functional behavior and elicit T cells, which can have regulatory activity...
  25. ncbi request reprint A Salmonella typhimurium effector protein SifA is modified by host cell prenylation and S-acylation machinery
    Anna T Reinicke
    Division of Immunology, Department of Pathology, Center for Veterinary Science, University of Cambridge, United Kingdom
    J Biol Chem 280:14620-7. 2005
    ..This is the only known example of a bacterial effector protein that is modified both by mammalian cell S-acylation and prenylation machinery...
  26. pmc Molecular interactions between desmosomal cadherins
    Shabih e Hassnain Syed
    Division of Membrane Biology, The National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    Biochem J 362:317-27. 2002
    ....
  27. ncbi request reprint Ras/ERK1/2-mediated STAT3 Ser727 phosphorylation by familial medullary thyroid carcinoma-associated RET mutants induces full activation of STAT3 and is required for c-fos promoter activation, cell mitogenicity, and transformation
    Ivan Plaza-Menacho
    Department of Genetics, Hanzeplein 1, 9700 RB, Groningen, The Netherlands
    J Biol Chem 282:6415-24. 2007
    ..These data show that FMTC-RET mutants activate a Ras/ERK1/2/STAT3 Ser(727) pathway, which plays an important role in cell mitogenicity and transformation...
  28. pmc Single-molecule diffusion reveals similar mobility for the Lck, H-ras, and K-ras membrane anchors
    Piet H M Lommerse
    Physics of Life Processes, Leiden Institute of Physics, Leiden University, Leiden, The Netherlands
    Biophys J 91:1090-7. 2006
    ....
  29. ncbi request reprint The Lck SH3 domain negatively regulates localization to lipid rafts through an interaction with c-Cbl
    Ibrahim Y Hawash
    Department of Biology, Purdue University, West Lafayette, Indiana 47907, USA
    J Biol Chem 277:5683-91. 2002
    ..The association of Lck with c-Cbl in vivo required a functional SH3 domain. These results suggest a model whereby the SH3 domain negatively regulates basal localization of Lck to lipid rafts via association with c-Cbl...