L L Hench

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..These results are encouraging, as cell adhesion is an important first step prior to proliferation and differentiation...

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..5-164.7 m(2)/g and a large fraction of 6-9 nm pore sizes. These differences in texture, as well as variations in chemical composition, account for significant changes in the resorption and in vivo responses...

..These responses were dependent upon Si and Na release profiles of the tape-cast discs, and development of the hydroxyapatite layer...

..This technique could have important applications in the field of regenerative medicine by enabling rapid characterization of cell or organoid behavior on novel bioactive scaffolds without damage to either cell or biomaterial...

..This result must be taken into account when carrying out in vitro cell-culture studies to simulate conditions in vivo and in experiments using extracts of the bioactive glass powders...

..These results indicate that the binary gel-derived CaO-SiO2 system exhibits a level of bioactivity over a similar molar range of SiO2 content as the previously studied ternary CaO-P2O5-SiO2 system...

..The identification of differentially expressed genes by cDNA microarray analysis has offered new insights into the mode of action of bioactive glasses and has proven to be an effective tool in evaluating their osteoproductive properties...

..Thus, particle size range, glass type, and powder volume fraction can be used as a means to control the release rate of active ions that stimulate the gene expression and cellular response for tissue proliferation and repair...

..This may have implications to bone tissue engineering using bioactive glasses...

..01% of that of the control culture. In comparison, BG did not possess antimicrobial properties over the concentration range investigated (0.1-40.0 mg/mL)...

..The differences in the 1190-1385 cm(-1) spectral region also suggest a decrease in the amount of nucleic acids and proteins. Using curve fitting, we quantify these spectral differences that can be used as markers of cell death...

..The surface area and mesopore volume decreased as polymer concentration increased in the hybrids. The strain at fracture of the hybrid foams was substantially greater than pure gel-glass foams...

..The development of a simple quality index has enabled some of the materials described within this paper to be ranked by their ability to replace bone, thus enabling possible new research directions to be emphasized...

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..e. implant/tissue stress-shielding, space-flight...

..These changes are the effects of the complex molecular mechanisms during the induction of cell death, such as protein cleavage due to the activation of caspases, followed by DNA fragmentation...

..At 14 days, nodule formation was observed, and these nodules stained positive for alizarin red, demonstrating Ca deposition and, therefore mineralization...

..The high bioactivity of the PDLLA foam/Bioglass composites indicates the potential of the materials for use as bioactive, resorbable scaffolds in bone tissue engineering...

..The data overall suggest that no significant effect can be ascribed to the ionic products of 58S bioactive gel-glass dissolution tested here and their ability to stimulate osteoblastic marker gene expression...

..Bioactive glass foam scaffolds sintered at 800 degrees C for 2 h fulfill the criteria for an ideal scaffold for tissue engineering applications...

..83 +/- 7% for dexamethasone alone). This study demonstrates the capacity of an entirely inorganic material to stimulate differentiation of ES cells toward a lineage with therapeutic potential in tissue-engineering applications...

..Conventional sol-gel processing yields pores an order of magnitude larger. Gelling time was also reduced from many hours to a few minutes, without using a gelling agent, and large crack-free monoliths were synthesized within 1 week...

..Bioactive glass scaffolds, containing 2 mol% silver, released silver ions at a rate that was similar to that which has previously been found to be bactericidal but not high enough to be cytotoxic to bone cells...

..The 70S30C bioactive glass scaffolds therefore fulfil many of the criteria for an ideal scaffold for bone tissue engineering applications...

..This paper describes how the structure and properties of the scaffolds are being optimized with respect to cell response and that tissue culture techniques must be optimized to enable growth of new bone in vitro...

..This report summarises the findings of the past and the present and also outlines potential of these calcium silicate gel-glasses in the field of biomaterials...

..These data demonstrate the bioactive effects of dissolution products derived from sol-gel materials on primary osteoblasts and complements in vivo studies that indicate the suitability of this material as a bone graft substitute...

..The in vitro dissolution and bioactivity of the bioactive foams were compared to that of unfoamed monoliths and powders (< 20 microm in diameter) of the same composition...

..It was shown that the simple two-component SiO2-CaO gel-glass powder is bioactive with comparable dissolution rates as the clinically used melt-derived 45S5 Bioglass powder and extensively studied sol-gel-derived 58S gel-glass powder...

..Undiluted dissolution products from the foams however caused significant apoptosis suggesting an ion concentration dependent response...

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..The apparent ability of 45S5 Bioglass incorporated into scaffolds to increase neovascularization would be extremely beneficial during the engineering of larger soft-tissue constructs...

..Our observations of the dissolution profiles of Ag(+) from AgBG in the presence and absence of bacteria are consistent with silver accumulation by the bacteria...

..Gene activation by controlled ion release provides the conceptual basis for molecular design of a third generation of biomaterials optimised for in situ tissue regeneration...

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..Secondly, the aim is to optimise the technique as a research tool for the non-invasive real time investigation of cell/material interactions in the fields of tissue engineering and toxicology testing...

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..The steps of discovery, characterization, in vivo and in vitro evaluation, clinical studies and product development are summarized along with the technology transfer processes...

..The membrane coated with silicon-containing HCA had much higher cell-proliferation ability than the membrane...

..This study proves the feasibility of using Raman spectroscopy to identify in situ phenotypic differences in living cells...

..These data suggest that 58S ionic dissolution products possibly mediate the bioactive effect of 58S through components of the IGF system and MAPK signaling pathways...