U Griesenbach

Summary

Affiliation: Imperial College
Country: UK

Publications

  1. ncbi request reprint Gene therapy progress and prospects: cystic fibrosis
    U Griesenbach
    Department of Gene Therapy, Imperial College at the National Heart and Lung Institute, London, UK
    Gene Ther 13:1061-7. 2006
  2. doi request reprint Secreted Gaussia luciferase as a sensitive reporter gene for in vivo and ex vivo studies of airway gene transfer
    Uta Griesenbach
    Department of Gene Therapy, Imperial College at the National Heart and Lung Institute, Manresa Road, London SW3 6LR, UK
    Biomaterials 32:2614-24. 2011
  3. doi request reprint Expert opinion in biological therapy: update on developments in lung gene transfer
    Uta Griesenbach
    National Heart and Lung Institute, Imperial College London, Department of Gene Therapy, and The UK Cystic Fibrosis Gene Therapy Consortium, London, UK
    Expert Opin Biol Ther 13:345-60. 2013
  4. doi request reprint Oral contraceptives do not appear to affect cystic fibrosis disease severity
    Natalie G Kernan
    Dept of Gene Therapy, Imperial College London, London, UK
    Eur Respir J 41:67-73. 2013
  5. doi request reprint Assessment of the nuclear pore dilating agent trans-cyclohexane-1,2-diol in differentiated airway epithelium
    Uta Griesenbach
    Department of Gene Therapy, Imperial College London, UK
    J Gene Med 14:491-500. 2012
  6. ncbi request reprint Progress in gene and cell therapy for cystic fibrosis lung disease
    Uta Griesenbach
    Department of Gene Therapy, Imperial College London, Emmanuel Kaye Building, Manresa Road, London
    Curr Pharm Des 18:642-62. 2012
  7. ncbi request reprint Sendai virus for gene therapy and vaccination
    Uta Griesenbach
    Imperial College London, NHLI, UK
    Curr Opin Mol Ther 7:346-52. 2005
  8. ncbi request reprint Effect of tolerance induction to immunodominant T-cell epitopes of Sendai virus on gene expression following repeat administration to lung
    U Griesenbach
    Department of Gene Therapy, NHLI, Imperial College, Edinburgh, London, UK
    Gene Ther 13:449-56. 2006
  9. pmc Inefficient cationic lipid-mediated siRNA and antisense oligonucleotide transfer to airway epithelial cells in vivo
    Uta Griesenbach
    Department of Gene Therapy, Faculty of Medicine, National Heart and Lung Institute, Imperial College, London, UK
    Respir Res 7:26. 2006
  10. doi request reprint Limitations of the murine nose in the development of nonviral airway gene transfer
    Uta Griesenbach
    Department of Gene Therapy, Imperial College at the National Heart and Lung Institute, Manresa Road, London SW3 6LR, UK
    Am J Respir Cell Mol Biol 43:46-54. 2010

Collaborators

Detail Information

Publications53

  1. ncbi request reprint Gene therapy progress and prospects: cystic fibrosis
    U Griesenbach
    Department of Gene Therapy, Imperial College at the National Heart and Lung Institute, London, UK
    Gene Ther 13:1061-7. 2006
    ..Developments in viral and non-viral vectors, as well as recent alternative strategies such as gene repair, trans-splicing and stem cell therapy will be reviewed...
  2. doi request reprint Secreted Gaussia luciferase as a sensitive reporter gene for in vivo and ex vivo studies of airway gene transfer
    Uta Griesenbach
    Department of Gene Therapy, Imperial College at the National Heart and Lung Institute, Manresa Road, London SW3 6LR, UK
    Biomaterials 32:2614-24. 2011
    ..This has allowed us to validate that GL67A, which is currently in clinical use, can generate significant amounts of recombinant protein in fully differentiated human air liquid interface cultures and the ovine lung in vivo...
  3. doi request reprint Expert opinion in biological therapy: update on developments in lung gene transfer
    Uta Griesenbach
    National Heart and Lung Institute, Imperial College London, Department of Gene Therapy, and The UK Cystic Fibrosis Gene Therapy Consortium, London, UK
    Expert Opin Biol Ther 13:345-60. 2013
    ..Although gene transfer to the lung has proven more challenging than initially anticipated, significant progress has been made over the last 10 years...
  4. doi request reprint Oral contraceptives do not appear to affect cystic fibrosis disease severity
    Natalie G Kernan
    Dept of Gene Therapy, Imperial College London, London, UK
    Eur Respir J 41:67-73. 2013
    ..Our data suggests that the use of OCs does not affect CF disease severity...
  5. doi request reprint Assessment of the nuclear pore dilating agent trans-cyclohexane-1,2-diol in differentiated airway epithelium
    Uta Griesenbach
    Department of Gene Therapy, Imperial College London, UK
    J Gene Med 14:491-500. 2012
    ..Previous studies have shown that TCHD can increase lipid-mediated transfection in vitro...
  6. ncbi request reprint Progress in gene and cell therapy for cystic fibrosis lung disease
    Uta Griesenbach
    Department of Gene Therapy, Imperial College London, Emmanuel Kaye Building, Manresa Road, London
    Curr Pharm Des 18:642-62. 2012
    ..Recent studies in viral and non-viral vector developments, as well as cell therapy will be discussed and an update on clinical gene therapy studies will be provided here...
  7. ncbi request reprint Sendai virus for gene therapy and vaccination
    Uta Griesenbach
    Imperial College London, NHLI, UK
    Curr Opin Mol Ther 7:346-52. 2005
    ..In addition, the potential of SeV vector for vaccination has been explored. Data on the use of SeV for gene therapy and vaccination since January 2004 are reviewed and recent improvements in SeV vectorology are discussed...
  8. ncbi request reprint Effect of tolerance induction to immunodominant T-cell epitopes of Sendai virus on gene expression following repeat administration to lung
    U Griesenbach
    Department of Gene Therapy, NHLI, Imperial College, Edinburgh, London, UK
    Gene Ther 13:449-56. 2006
    ..Multiple immune mechanisms operate to eradicate viruses from the lung, and these findings indicate that impeding the adaptive T-cell response to the immunodominant viral epitope is not sufficient to prevent the process...
  9. pmc Inefficient cationic lipid-mediated siRNA and antisense oligonucleotide transfer to airway epithelial cells in vivo
    Uta Griesenbach
    Department of Gene Therapy, Faculty of Medicine, National Heart and Lung Institute, Imperial College, London, UK
    Respir Res 7:26. 2006
    ..The cationic lipid Genzyme lipid (GL) 67 is the current "gold-standard" for in vivo lung gene transfer. Here, we assessed, if GL67 mediated uptake of siRNAs and asODNs into airway epithelium in vivo...
  10. doi request reprint Limitations of the murine nose in the development of nonviral airway gene transfer
    Uta Griesenbach
    Department of Gene Therapy, Imperial College at the National Heart and Lung Institute, Manresa Road, London SW3 6LR, UK
    Am J Respir Cell Mol Biol 43:46-54. 2010
    ..At the current levels of gene transfer efficiency achievable with nonviral vectors, the murine nose is of limited value as a stepping stone to human trials...
  11. ncbi request reprint In vivo imaging of gene transfer to the respiratory tract
    Uta Griesenbach
    Department of Gene Therapy, Imperial College at the National Heart and Lung Institute, Manresa Road, London SW3 6LR, UK
    Biomaterials 29:1533-40. 2008
    ..45, p<0.05). To our knowledge these studies show for the first time that this non-invasive method of assessing pulmonary gene transfer is viable for evaluating non-viral gene transfer agents...
  12. doi request reprint The role of doxorubicin in non-viral gene transfer in the lung
    Uta Griesenbach
    Department of Gene Therapy, Imperial College at the National Heart and Lung Institute, Manresa Road, London SW36LR, UK
    Biomaterials 30:1971-7. 2009
    ....
  13. doi request reprint Gene transfer to the lung: lessons learned from more than 2 decades of CF gene therapy
    Uta Griesenbach
    Department of Gene Therapy, Faculty of Medicine at the National Heart and Lung Institute, Imperial College London, Manresa Road, London SW36LR, UK
    Adv Drug Deliv Rev 61:128-39. 2009
    ..This review will focus on CF as an example for lung gene therapy, but lessons learned may be applicable to other target diseases...
  14. doi request reprint Validation of nasal potential difference measurements in gut-corrected CF knockout mice
    Uta Griesenbach
    Department of Gene Therapy, Faculty of Medicine, National Heart and Lung Institute, Imperial College London, London, United Kingdom
    Am J Respir Cell Mol Biol 39:490-6. 2008
    ..These data should allow a more informed use of CF animals in future studies...
  15. doi request reprint The use of carboxymethylcellulose gel to increase non-viral gene transfer in mouse airways
    Uta Griesenbach
    Department of Gene Therapy, Imperial College at the National Heart and Lung Institute, London SW3 6LR, UK
    Biomaterials 31:2665-72. 2010
    ..0001, n=18). This study suggests that contact time of non-viral gene transfer agents is a key factor for gene delivery, and suggests two methods which may be translatable for use in man...
  16. ncbi request reprint Pre-clinical and clinical endpoint assays for cystic fibrosis gene therapy
    Uta Griesenbach
    Department of Gene Therapy, Faculty of Medicine, Imperial College London, UK
    J Cyst Fibros 4:89-100. 2005
    ....
  17. doi request reprint Quantification of periciliary fluid height in human airway biopsies is feasible, but not suitable as a biomarker
    Uta Griesenbach
    Department of Gene Therapy, Imperial College London, UK
    Am J Respir Cell Mol Biol 44:309-15. 2011
    ..However, power calculations indicate that this assay can only be considered as a biomarker in large, late-phase clinical trials, because sample sizes required to achieve sufficient power are comparatively large...
  18. ncbi request reprint Advances in cystic fibrosis gene therapy
    Uta Griesenbach
    Department of Gene Therapy, Faculty of Medicine, National Heart and Lung Institute, Imperial College, London, UK
    Curr Opin Pulm Med 10:542-6. 2004
    ..The authors review the most recent advances in preclinical airway gene transfer and summarize the results from the latest clinical trials...
  19. ncbi request reprint Cystic fibrosis gene therapy: successes, failures and hopes for the future
    Uta Griesenbach
    Department of Gene Therapy, Faculty of Medicine at the National Heart and Lung Institute, Imperial College London, Manresa Road, London SW3 6LR, UK
    Expert Rev Respir Med 3:363-71. 2009
    ..Here, we will summarize the key findings of these clinical studies and describe current preclinical and clinical research aimed at further developing gene therapy for CF...
  20. ncbi request reprint Update on gene therapy for cystic fibrosis
    Uta Griesenbach
    Department of Gene Therapy, Faculty of Medicine, National Heart and Lung Institute, Imperial College London, Manresa Road, London, SW3 6LR, UK
    Curr Opin Mol Ther 5:489-94. 2003
    ..Steady progress has been made over the last two years and key papers, including recent advances in viral and nonviral gene transfer agents, will be reviewed here...
  21. doi request reprint Validation of recombinant Sendai virus in a non-natural host model
    U Griesenbach
    Department of Gene Therapy, Imperial College London, National Heart and Lung Institute, Manresa Road, London, UK
    Gene Ther 18:182-8. 2011
    ..In conclusion, the SeV vector should be strongly considered for lung-related applications requiring a single administration of the vector even though it might not be suitable for diseases requiring repeat administration...
  22. doi request reprint Current status and future directions of gene and cell therapy for cystic fibrosis
    Uta Griesenbach
    Department of Gene Therapy, Imperial College London, London, UK
    BioDrugs 25:77-88. 2011
    ..In this review, we discuss recent developments with viral and non-viral vectors and cell therapy, and provide an update on clinical gene therapy studies...
  23. ncbi request reprint Use of ultrasound to enhance nonviral lung gene transfer in vivo
    S Xenariou
    Department of Gene Therapy, National Heart and Lung Institute, Faculty of Medicine, Imperial College, London, UK
    Gene Ther 14:768-74. 2007
    ..We have thus established proof of principle that US can increase nonviral gene transfer, in the air-filled murine lung...
  24. ncbi request reprint Intravenously administered oligonucleotides can be delivered to conducting airway epithelium via the bronchial circulation
    E Holder
    Department of Gene Therapy, Faculty of Medicine, Imperial College, Manrisa Road, London, UK
    Gene Ther 13:1628-38. 2006
    ..ODNs may be relevant to CF in a variety of ways and these data suggest one way towards implementing their use...
  25. ncbi request reprint Inflammation in cystic fibrosis airways: relationship to increased bacterial adherence
    P Scheid
    Dept. of Gene Therapy, Imperial College at the National Heart and Lung Institute, London, UK
    Eur Respir J 17:27-35. 2001
    ..The authors conclude that the stimulus produced by Pseudomonas aeruginosa is the predominant inflammatory trigger in their models...
  26. ncbi request reprint Sendai virus-mediated CFTR gene transfer to the airway epithelium
    S Ferrari
    Department of Gene Therapy, Faculty of Medicine, Imperial College, National Heart and Lung Institute, London, UK
    Gene Ther 14:1371-9. 2007
    ..Further modifications to the vector or the host may make it easier to translate these studies into clinical trials of cystic fibrosis...
  27. ncbi request reprint A defective nontransmissible recombinant Sendai virus mediates efficient gene transfer to airway epithelium in vivo
    S Ferrari
    Department of Gene Therapy, National Heart and Lung Institute, Imperial College Faculty of Medicine, London, UK
    Gene Ther 11:1659-64. 2004
    ....
  28. pmc Bactofection of lung epithelial cells in vitro and in vivo using a genetically modified Escherichia coli
    M D B Larsen
    Department of Gene Therapy, Faculty of Medicine, National Heart and Lung Institute, Imperial College, London, UK
    Gene Ther 15:434-42. 2008
    ..In conclusion, although proof-of-principle for lung bactofection has been demonstrated, levels were low and further modification to the bacterial vector, vector administration and the plasmids will be required...
  29. ncbi request reprint Gene therapy progress and prospects: cystic fibrosis
    U Griesenbach
    Department of Gene Therapy, National Heart and Lung Institute, Imperial College, Faculty of Medicine, London, UK
    Gene Ther 9:1344-50. 2002
    ..Here, we will review the clinical and pre-clinical progress for the last 2 years (2000-2001) and briefly speculate on future prospects for the next 2 in CF gene therapy...
  30. ncbi request reprint Using magnetic forces to enhance non-viral gene transfer to airway epithelium in vivo
    S Xenariou
    Department of Gene Therapy, National Heart and Lung Institute, Faculty of Medicine, Imperial College, 1B Manresa Road, London SW3 6LR, UK
    Gene Ther 13:1545-52. 2006
    ..Thus, whereas exposure to a magnetic field improved in vitro transfection efficiency, translation to the in vivo setting remains difficult...
  31. pmc Immunological hurdles to lung gene therapy
    S Ferrari
    Department of Gene Therapy, National Heart and Lung Institute, Imperial College Faculty of Medicine, London, UK
    Clin Exp Immunol 132:1-8. 2003
    ..A better understanding of the immunological barriers which exist in the lung might allow for a more sustained expression of the transgene and importantly help overcome the problem of readministration of viral vectors...
  32. ncbi request reprint Gene therapy for cystic fibrosis: an example for lung gene therapy
    U Griesenbach
    Department of Gene Therapy, Faculty of Medicine at the National Heart and Lung Institute, Imperial College, London, UK
    Gene Ther 11:S43-50. 2004
    ..This review will focus on CF as an example for lung gene therapy and discuss the progress made in this field over the last couple of years...
  33. ncbi request reprint Recent progress in gene therapy for cystic fibrosis
    U Griesenbach
    Department of Gene Therapy, Imperial College School of Medicine, National Heart and Lung Institute, London, UK
    Curr Opin Mol Ther 3:385-9. 2001
    ..Here, we review the progress in CF gene therapy over the last 12 months, including recent advances in viral and non-viral gene transfer agents and novel strategies, such as RNA repair and stem cell gene therapy...
  34. ncbi request reprint Effects of intramyocardial pVEGF165 delivery on regional myocardial blood flow: evidence for a spatial 'delivery-efficacy' mismatch
    P W Radke
    Department of Gene Therapy, National Heart and Lung Institute, Faculty of Medicine, Imperial College London, UK
    Gene Ther 11:1249-55. 2004
    ..These data, therefore, demonstrate a spatial 'delivery-efficacy' mismatch with implications for myocardial gene delivery sites and detection of treatment effects in vivo...
  35. ncbi request reprint Cytoplasmic deposition of NFkappaB decoy oligonucleotides is insufficient to inhibit bleomycin-induced pulmonary inflammation
    U Griesenbach
    Department of Gene Therapy, Faculty of Medicine, Imperial College, London, UK
    Gene Ther 9:1109-15. 2002
    ..We suggest that access of ODN to the nucleus of airway epithelial cells is a key problem, limiting the efficacy of such decoy strategies, as well as attempts at gene repair...
  36. doi request reprint Detection of CFTR transgene mRNA expression in respiratory epithelium isolated from the murine nasal cavity
    Emma Holder
    Medical Genetics Section, Molecular Medicine Centre, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, UK
    J Gene Med 12:55-63. 2010
    ..Only the respiratory epithelium is a satisfactory model for human airway epithelium and therefore CFTR gene transfer should be specifically assessed in respiratory epithelial cells (RECs)...
  37. pmc Cystic fibrosis modifier genes
    Jane Davies
    Department of Gene Therapy, Faculty of Medicine, Imperial College, Emmanuel Kaye Building, Manresa Road, London, UK
    J R Soc Med 98:47-54. 2005
    ..The aims of such studies are to increase our understanding of disease pathogenesis, to aid prognosis and ultimately to lead to the development of novel treatments...
  38. ncbi request reprint Optimizing aerosol gene delivery and expression in the ovine lung
    Gerry McLachlan
    Medical Genetics Section, School of Molecular and Clinical Medicine, University of Edinburgh, Western General Hospital, Edinburgh, UK
    Mol Ther 15:348-54. 2007
    ..Dose-related toxicity of GTA was reduced by aerosol administration compared to direct instillation. This large animal model will allow us to move toward clinical studies with greater confidence...
  39. pmc Identification and functional characterization of cytoplasmic determinants of plasmid DNA nuclear import
    Felix M Munkonge
    Department of Gene Therapy, National Heart and Lung Institute, Faculty of Medicine, Imperial College London, London SW3 6LR, United Kingdom
    J Biol Chem 284:26978-87. 2009
    ..Increasing the potential for exogenously added pDNA to bind intracellular transport cofactors may enhance the potency of non-viral gene transfer...
  40. doi request reprint Nasal abnormalities in cystic fibrosis mice independent of infection and inflammation
    Tom N Hilliard
    Department of Gene Therapy, Emmanuel Kaye Building, 1B Manresa Road, London SW3 6LR, UK
    Am J Respir Cell Mol Biol 39:19-25. 2008
    ..There are significant histologic changes in the nasal mucosa of adult CF mice, not associated with increased lumenal inflammation or bacterial content, and which are not present perinatally. These may be novel therapeutic targets...
  41. doi request reprint Assessment of CFTR function after gene transfer in vitro and in vivo
    Uta Griesenbach
    Department of Gene Therapy, Faculty of Medicine, Imperial College London, UK
    Methods Mol Biol 433:229-42. 2008
    ..Here, we describe pre-clinical methods related to assessing correction of the CF chloride transport defect...
  42. ncbi request reprint The nasal epithelium as a factory for systemic protein delivery
    Uta Griesenbach
    Department of Gene Therapy, Imperial College School of Medicine at the National Heart and Lung Institute, London, SM5 1RU, UK
    Mol Ther 5:98-103. 2002
    ..The combination of a high-efficiency gene transfer agent (SeV) and sites that can be assessed noninvasively (nose or lung) may circumvent several current challenges to gene therapy...
  43. ncbi request reprint Emerging significance of plasmid DNA nuclear import in gene therapy
    Felix M Munkonge
    Department of Gene Therapy, National Heart and Lung Institute, Faculty of Medicine, Imperial College, London, UK
    Adv Drug Deliv Rev 55:749-60. 2003
    ....
  44. doi request reprint Toxicology study assessing efficacy and safety of repeated administration of lipid/DNA complexes to mouse lung
    E W F W Alton
    1 UK CF Gene Therapy Consortium, London, Oxford and Edinburgh, UK 2 Department of Gene Therapy, Imperial College, London, UK
    Gene Ther 21:89-95. 2014
    ..This study supports progression into the first non-viral multidose lung trial in CF patients. ..
  45. ncbi request reprint Modifier genes in cystic fibrosis
    J C Davies
    Department of Gene Therapy, National Lung and Heart Institute, Faculty of Medicine, Imperial College, London, UK
    Pediatr Pulmonol 39:383-91. 2005
    ..This review will summarize the field to date and discuss some of the methodological issues important in the design and interpretation of such studies...
  46. ncbi request reprint Progress and prospects: gene therapy clinical trials (part 2)
    Eric Alton
    Department of Gene Therapy, Emmanuel Kaye Building, NHLI, Imperial College, Manresa Road, London, UK
    Gene Ther 14:1555-63. 2007
    ..This part includes clinical trials for skin diseases, neurological disorders, HIV/AIDS, ornithine transcarbamylase deficiency, alpha(1)-antitrypsin deficiency, haemophilia and cancer...
  47. ncbi request reprint Low-frequency ultrasound increases non-viral gene transfer to the mouse lung
    Stefania Xenariou
    Department of Gene Therapy, National Heart and Lung Institute, Faculty of Medicine, Imperial College, London, UK
    Acta Biochim Biophys Sin (Shanghai) 42:45-51. 2010
    ..We have thus, established that low-frequency US can enhance lung gene transfer with naked pDNA and this enhancement is more effective than the previously reported 1 MHz US...
  48. ncbi request reprint Critical appraisal of the mouse model of myocardial infarction
    Naomi M Degabriele
    Department of Gene Therapy, Faculty of Medicine at the National Heart and Lung Institute, Imperial College, London, UK
    Exp Physiol 89:497-505. 2004
    ..Power calculations indicated that, despite a certain amount of intragroup variation, the Middle Suture model may be useful for therapeutic studies to assess the effects of treatment on cardiac function and overall lesion size...
  49. ncbi request reprint Vascular oligonucleotide transfer facilitated by a polymer-coated stent
    Peter W Radke
    Department of Gene Therapy, National Heart and Lung Institute, Faculty of Medicine, Imperial College London, SW3 6LR, UK
    Hum Gene Ther 16:734-40. 2005
    ..Rapid intravascular release of ODN before implantation and potential vascular barriers for gene transfer are most likely responsible for the currently unsatisfactory in vivo release kinetics...
  50. ncbi request reprint CFTR gene transfer to human cystic fibrosis pancreatic duct cells using a Sendai virus vector
    Zoltan Rakonczay
    Institute for Cell and Molecular Biosciences, University of Newcastle, Newcastle upon Tyne, United Kingdom
    J Cell Physiol 214:442-55. 2008
    ..Our data show that SeV vector is a potential CFTR gene transfer agent for human pancreatic duct cells and that expression of CFTR in CF cells is associated with a restoration of Cl- and HCO3- transport at the apical membrane...
  51. doi request reprint CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression
    Stephen C Hyde
    Gene Medicine Research Group, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, Oxford OX3 9DU, UK
    Nat Biotechnol 26:549-51. 2008
    ..Using a CpG-free pDNA expression vector, we achieved sustained (>or=56 d) in vivo transgene expression in the absence of lung inflammation...
  52. ncbi request reprint Transfection efficiency and toxicity following delivery of naked plasmid DNA and cationic lipid-DNA complexes to ovine lung segments
    Michael Emerson
    Medical Genetics Section, School of Molecular and Clinical Medicine, and Department of Veterinary Pathology, College of Medicine and Veterinary Medicine, University of Edinburg, Edinburgh EH8 9AG, United Kingdom
    Mol Ther 8:646-53. 2003
    ..The severity of the inflammatory response appeared to correlate with the administered dose of DNA and was generally more severe for pDNA:GL67...
  53. pmc Beta-defensin genomic copy number is not a modifier locus for cystic fibrosis
    Edward J Hollox
    Institute of Genetics, University of Nottingham, Nottingham, UK
    J Negat Results Biomed 4:9. 2005
    ..No significant association was found...