Paul S Freemont
Affiliation: Imperial College
Planamente S, Salih O, Manoli E, Albesa Jové D, Freemont P, Filloux A. TssA forms a gp6-like ring attached to the type VI secretion sheath. EMBO J. 2016;35:1613-27 pubmed publisher
..These observations, combined with similarities between TssF and gp6N-terminus or TssG and gp53, lead us to propose a comparative model between the phage baseplate and the T6SS. ..
Goers L, Freemont P, Polizzi K. Co-culture systems and technologies: taking synthetic biology to the next level. J R Soc Interface. 2014;11: pubmed publisher
..Greater focus on foundational technology developments for co-cultures is needed for many synthetic biology systems to realize their potential in both applications and answering biological questions. ..
Campeotto I, Percy M, MacDonald J, Forster A, Freemont P, GRUNDLING A. Structural and mechanistic insight into the Listeria monocytogenes two-enzyme lipoteichoic acid synthesis system. J Biol Chem. 2014;289:28054-69 pubmed publisher
..Mutational analysis confirmed an essential function for this binding site and allowed us to propose a model for the binding of the growing chain. ..
Weston D, Russell R, Batty E, Jensen K, Stephens D, Adams N, et al
. New quantitative approaches reveal the spatial preference of nuclear compartments in mammalian fibroblasts. J R Soc Interface. 2015;12:20140894 pubmed publisher
..This new methodology is thus able to reveal the effect of large-scale perturbation on spatial architecture and preferences that would not be obvious from single cell imaging. ..
Moore S, MacDonald J, Wienecke S, Ishwarbhai A, Tsipa A, Aw R, et al
. Rapid acquisition and model-based analysis of cell-free transcription-translation reactions from nonmodel bacteria. Proc Natl Acad Sci U S A. 2018;115:E4340-E4349 pubmed publisher
Lai H, Moore S, Polizzi K, Freemont P. EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. Methods Mol Biol. 2018;1772:429-444 pubmed publisher
..A secondary module cloning site is also available for pathway optimization, in order to limit library size if necessary. Here, we show the utility of EcoFlex using the violacein biosynthesis pathway as an example. ..
Salih O, He S, Planamente S, Stach L, MacDonald J, Manoli E, et al
. Atomic Structure of Type VI Contractile Sheath from Pseudomonas aeruginosa. Structure. 2018;26:329-336.e3 pubmed publisher
..We used the P. aeruginosa R2 pyocin as a structural template to build an atomic model of the TssB1C1 sheath in its extended conformation, allowing us to propose a coiled-spring-like mechanism for T6SS sheath contraction. ..
Campeotto I, Zhang Y, Mladenov M, Freemont P, GrÃ¼ndling A. Complex structure and biochemical characterization of the Staphylococcus aureus cyclic diadenylate monophosphate (c-di-AMP)-binding protein PstA, the founding member of a new signal transduction protein family. J Biol Chem. 2015;290:2888-901 pubmed publisher
Kelwick R, Webb A, MacDonald J, Freemont P. Development of a Bacillus subtilis cell-free transcription-translation system for prototyping regulatory elements. Metab Eng. 2016;38:370-381 pubmed publisher
..Additionally, we demonstrate the cell-free characterisation of an inducible expression system, and the activity of a model enzyme - renilla luciferase. ..