Research Topics
| Andrew J deMelloSummaryAffiliation: Imperial College Country: UK Publications
| Collaborators
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Detail Information
Publications
Control and detection of chemical reactions in microfluidic systemsAndrew J deMello
Electronic Materials Group, Department of Chemistry, Imperial College London, Exhibition Road, South Kensington, London, SW7 2AZ, UK
Nature 442:394-402. 2006..Such systems define new operational paradigms and provide predictions about how molecular synthesis might be revolutionized in the fields of high-throughput synthesis and chemical production...- Precise temperature control in microfluidic devices using Joule heating of ionic liquidsAndrew J de Mello
Imperial College London, Exhibition Road, South Kensington, London, UK SW7 2AZ
Lab Chip 4:417-9. 2004..2 degrees C. Ionic liquids held in co-running channels are Joule heated with an a.c. current. The nature of the devices means that the internal temperature can be directly assessed in a facile manner... 
Passive self-synchronized two-droplet generationJongin Hong
Department of Chemistry, Imperial College London, South Kensington, London, SW7 2AZ, UK
Lab Chip 10:2702-9. 2010..We study droplet generation and self-synchronization in a quantitative fashion by using high-speed image analysis...- Lab-chip HPLC with integrated droplet-based microfluidics for separation and high frequency compartmentalisationJin Young Kim
Department of Bioengineering, Imperial College London, London SW7 2AZ, UK
Chem Commun (Camb) 48:9144-6. 2012..This describes the use of droplet-based microfluidics for the preservation of chromatographic separations, and its potential application as a high frequency fraction collector... - Analysis of protein-protein interactions by using droplet-based microfluidicsMonpichar Srisa-art
Department of Chemistry, Imperial College London, Exhibition Road, London, UK
Chembiochem 10:1605-11. 2009..Importantly, the use of fluorophores does not affect the activity of ANG or the binding of anti-ANG antibodies to ANG. Such an experimental platform could be applied to the high-throughput analysis of protein-protein interactions... 
Opportunities for microfluidic technologies in synthetic biologyShelly Gulati
Department of Chemistry, Imperial College London, London, UK
J R Soc Interface 6:S493-506. 2009..Recent advances in the field of microfluidics are reviewed and the potential of such a technological platform to support the rapid development of synthetic biology solutions is discussed...- Mapping of fluidic mixing in microdroplets with 1 micros time resolution using fluorescence lifetime imagingXavier Casadevall i Solvas
Department of Chemistry, Imperial College London, South Kensington, London, SW7 2AZ, United Kingdom
Anal Chem 82:3950-6. 2010..Importantly, such exquisite resolution is only possible as a result of the large number of droplets sampled and their high structural reproducibility... - Dielectric cell response in highly conductive buffersFabrice Gielen
Department of Chemistry, Imperial College London, South Kensington, London, SW7 2AZ, United Kingdom
Anal Chem 84:1849-53. 2012..Herein, we demonstrate that at 40 MHz, we are able to statistically distinguish between live and dead cell populations... - Novel technologies for the formation of 2-D and 3-D droplet interface bilayer networksYuval Elani
Department of Chemistry, Imperial College London, Exhibition Road South Kensington, London, UK
Lab Chip 12:3514-20. 2012..Both approaches allow individual droplet position and composition to be controlled, paving the way for complex on-chip functional network synthesis... - Thermoset polyester droplet-based microfluidic devices for high frequency generationJin Young Kim
Department of Bioengineering, Imperial College London, London, United Kingdom
Lab Chip 11:4108-12. 2011..The improved resistance of TPE to high pressures enabled investigation of high frequency droplet generation as a function of a wide range of flow-rates with three different oils as continuous phase... - Non-emissive colour filters for fluorescence detectionMikihide Yamazaki
Department of Chemistry, Imperial College London, South Kensington, London, United Kingdom
Lab Chip 11:1228-33. 2011.... - Identification of rare progenitor cells from human periosteal tissue using droplet microfluidicsMonpichar Srisa-art
Department of Chemistry, Imperial College London, South Kensington, London, UK SW7 2AZ
Analyst 134:2239-45. 2009..Accordingly, this study demonstrates the biological capacity of droplet-based microfluidics for cellular analysis and provides a necessary first step towards the development of a novel cell sorting technology... - Bio-electrospraying and droplet-based microfluidics: control of cell numbers within living residuesJongin Hong
Department of Chemistry, Imperial College London, UK
Biomed Mater 5:21001. 2010..In this communication, we address this issue by demonstrating the coupling of BES with droplet-based microfluidics for controlling live cell numbers within droplets and residues... - High-resolution local imaging of temperature in dielectrophoretic platformsFabrice Gielen
Institute of Biomedical Engineering, Imperial College London, South Kensington, London, SW7 2AZ, United Kingdom
Anal Chem 82:7509-14. 2010..Such measurements are shown to provide a novel calibration tool for screening temperature-mediated processes with high resolution... - Increasing the trapping efficiency of particles in microfluidic planar platforms by means of negative dielectrophoresisFabrice Gielen
Department of Chemistry, Institute of Biomedical Engineering, and Chemical Biology Centre, Imperial College London, South Kensington, London, SW7 2AZ, United Kingdom
J Phys Chem B 113:1493-500. 2009..This trapping configuration results in improved trapping yields and a decrease in overall reagent consumption. Particles are trapped dynamically while flowing in a microfluidic channel... - Pillar-induced droplet merging in microfluidic circuitsXize Niu
Department of Chemistry, Imperial College London, South Kensington, London, UKSW7 2AZ
Lab Chip 8:1837-41. 2008..Finally, we note that the merging of droplet interfaces occurs within both compressing and the decompressing regimes... - Resizing metal-coated nanopores using a scanning electron microscopeGuillaume A T Chansin
Department of Chemistry, Imperial College London, London, SW7 2AZ, UK
Small 7:2736-41. 2011..Shrinkage is observed on the Al side of the pore as well as on the Si(3) N(4) side, while the shrinkage rate is observed to be dependent on a variety of factors... - Micro- and nanofluidic systems for high-throughput biological screeningJongin Hong
Department of Chemistry, Imperial College London, South Kensington Campus, London SW7 2AZ, United Kingdom
Drug Discov Today 14:134-46. 2009..We review recent advances in both arraying strategies based on nano/microfluidics and novel nano/microfluidic devices with high analytical throughput rates... - A microdroplet dilutor for high-throughput screeningXize Niu
Department of Chemistry, Imperial College London, Exhibition Road, South Kensington, London SW72AZ, UK
Nat Chem 3:437-42. 2011..As a proof of concept, we used the dilutor to perform a high-throughput homogeneous DNA-binding assay using only nanolitres of sample... - Highly sensitive fluorescence detection system for microfluidic lab-on-a-chipGihan Ryu
Molecular Vision Ltd BioIncubator Unit, Bessemer Building, Imperial College London, London, SW7 2BP, UK
Lab Chip 11:1664-70. 2011..6 × 10(4) beads µl(-1) which can be equated to ∼3 nM fluorescein equivalent concentration. The LOD for the human plasma immunoassays is measured as 1.5 ng ml(-1) for both myoglobin and CK-MB... - Single-molecule spectroscopy using nanoporous membranesGuillaume A T Chansin
Institute of Biomedical Engineering, Imperial College London, South Kensington, SW7 2AZ, United Kingdom
Nano Lett 7:2901-6. 2007..Single-pore translocation events are also successfully detected using single-point confocal spectroscopy... - Fluorescence lifetime imaging of mixing dynamics in continuous-flow microdroplet reactorsMonpichar Srisa-art
Department of Chemistry, Imperial College London, South Kensington, London SW7 2AZ, United Kingdom
Phys Rev Lett 101:014502. 2008..Herein, we demonstrate that fluorescence lifetime imaging can be used to reconstruct mixing patterns within a droplet with a time resolution of 5 micros... - Monitoring of real-time streptavidin-biotin binding kinetics using droplet microfluidicsMonpichar Srisa-art
Department of Chemistry, Imperial College London, South Kensington, London, SW7 2AZ, United Kingdom
Anal Chem 80:7063-7. 2008..The binding rate constant of streptavidin and biotin was found to be in a range of 3.0 x 10 (6)-4.5 x 10 (7) M (-1) s (-1)... - High-throughput age synchronisation of Caenorhabditis elegansXavier Casadevall i Solvas
Department of Chemistry, Imperial College London, London, UK
Chem Commun (Camb) 47:9801-3. 2011..In brief, the microfluidic device allows worms to sort themselves in a passive manner... - Design of a solid-state nanopore-based platform for single-molecule spectroscopyJongin Hong
Department of Chemistry, Imperial College London, South Kensington, London SW7 2AZ, UK
Nanotechnology 19:165205. 2008..The cut-off behavior for Al/SiN membranes with varying pore diameters was investigated in terms of light propagation, distribution of electromagnetic fields, and light attenuation characteristics... - Electro-coalescence of digitally controlled dropletsXize Niu
Department of Chemistry, and Institute of Biomedical Engineering, Imperial College London, Exhibition Road, South Kensington, London SW7 2AZ, United Kingdom
Anal Chem 81:7321-5. 2009..This brings adjacent droplets into close proximity. At this point, an electric field applied to the electrodes breaks up the thin oil film surrounding the droplets resulting in merging... - A first step towards practical single cell proteomics: a microfluidic antibody capture chip with TIRF detectionAli Salehi-Reyhani
Single Cell Proteomics Project, Institute of Chemical Biology, Imperial College London, Exhibition Road, London SW7 2AZ, UK
Lab Chip 11:1256-61. 2011..These results suggest that this is a viable method for measuring relative protein levels in single cells... - Integrated thin-film polymer/fullerene photodetectors for on-chip microfluidic chemiluminescence detectionXuhua Wang
Experimental Solid State Physics Group, Blackett Laboratory, Imperial College London, UK
Lab Chip 7:58-63. 2007.... - High-efficiency single-molecule detection within trapped aqueous microdropletsMonpichar Srisa-art
Department of Chemistry, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, Pathumwan, Bangkok, 10330 Thailand
J Phys Chem B 114:15766-72. 2010..This circulation phenomenon allows a given molecule to be detected multiple times during an experiment and can therefore be used for performing time-dependent single-molecule analysis... - Affinity chromatography and capillary electrophoresis for analysis of the yeast ribosomal proteinsMiriam S Goyder
The Single Cell Proteomics Group, Institute of Chemical Biology, Department of Chemistry, Imperial College London, South Kensington, London, UK
BMB Rep 45:233-8. 2012..This method is a remarkably quick route from cell to separation that has the potential to be coupled to high throughput readout platforms for studies of the ribosomal proteome... - High-throughput DNA droplet assays using picoliter reactor volumesMonpichar Srisa Art
Department of Chemistry and Institute of Biomedical Engineering, Imperial College London, South Kensington, London, UK
Anal Chem 79:6682-9. 2007..FRET efficiency for this FRET pair was also investigated from the binding results. Efficiency results show that this detection system can precisely measure FRET even at low FRET efficiencies... - Quantitative 3D mapping of fluidic temperatures within microchannel networks using fluorescence lifetime imagingRichard K P Benninger
Department of Physics and Department of Chemistry, Imperial College London, Exhibition Road, South Kensington, London, SW7 2AZ, United Kingdom
Anal Chem 78:2272-8. 2006..This technique allows optimization of the chip design for miniaturized processes, such as on-chip PCR, for which precise temperature control is important... - Thin-film polymer light emitting diodes as integrated excitation sources for microscale capillary electrophoresisJoshua B Edel
Department of Chemistry, Imperial College London, South Kensington, London, UKSW7 2AZ
Lab Chip 4:136-40. 2004..The drive voltages required to generate sufficient emission from the polymer diode device are as low as 3.7 V... - In-column field-amplified sample stacking of biogenic amines on microfabricated electrophoresis devicesNigel P Beard
Department of Chemistry, Imperial College of Science, Technology and Medicine, South Kensington, London, UK
Electrophoresis 24:732-9. 2003..This design feature radically reduces the complexity in chip structures and associated chip operation. The approach is applied to the analysis of fluorescently labelled biogenic amines affording detection at concentrations down to 20 pM... - Discrimination between single Escherichia coli cells using time-resolved confocal spectroscopyJoshua B Edel
Institute of Biomedical Engineering, Department of Chemistry, South Kensington, London, SW7 2AZ, United Kingdom
J Phys Chem B 111:1129-34. 2007..The simplicity of the approach for obtaining well-defined burst width distributions is expected to be extremely valuable for single-cell sorting experiments... - Microfluidics: DNA amplification moves onAndrew J deMello
Nature 422:28-9. 2003 - Towards microalbuminuria determination on a disposable diagnostic microchip with integrated fluorescence detection based on thin-film organic light emitting diodesOliver Hofmann
Molecular Vision Ltd, 90 Fetter Lane, London, EC4A 1JP, United Kingdom
Lab Chip 5:863-8. 2005..This sensitivity is sufficient for the determination of microalbuminuria (MAU), an increased urinary albumin excretion indicative of renal disease (clinical cut-off levels: 15-40 mg L(-1))... - Monolithically integrated dye-doped PDMS long-pass filters for disposable on-chip fluorescence detectionOliver Hofmann
Molecular Vision Ltd, 90 Fetter Lane, London EC4A 1JP, UK
Lab Chip 6:981-7. 2006..The provision of low cost high quality integrated filters represents a key step towards the development of high-sensitivity disposable microfluidic devices for point-of-care diagnostics...