Genomes and Genes
John R Couchman
Affiliation: Imperial College
- Syndecans: proteoglycan regulators of cell-surface microdomains?John R Couchman
Division of Biomedical Sciences, Faculty of Medicine, Imperial College London, Exhibition Road, London SW7 2AZ, UK
Nat Rev Mol Cell Biol 4:926-37. 2003
- Serine 34 phosphorylation of rho guanine dissociation inhibitor (RhoGDIalpha) links signaling from conventional protein kinase C to RhoGTPase in cell adhesionAthanassios Dovas
Division of Biomedical Sciences, Faculty of Medicine, Imperial College London, Exhibition Road, London SW7 2AZ, United Kingdom
J Biol Chem 285:23296-308. 2010..Together with fluorescence resonance energy transfer microscopy sensing GTP-RhoA levels, the data reveal a common pathway in cell adhesion linking two essential mediators, conventional PKC and RhoA...
- Syndecans promote integrin-mediated adhesion of mesenchymal cells in two distinct pathwaysJames R Whiteford
National Heart and Lung Institute, Faculty of Medicine, Imperial College London, Sir Alexander Fleming Building, Exhibition Road, London SW7 2AZ, UK
Exp Cell Res 313:3902-13. 2007..Evidence for an indirect interaction of beta1 integrin with both syndecan ectodomains was obtained, all of which suggests a distinct mechanism of integrin-mediated adhesion...
- Fibronectin matrix assembly requires distinct contributions from Rho kinases I and -IIAtsuko Yoneda
Division of Biomedical Sciences, Imperial College London, London SW7 2AZ, United Kingdom
Mol Biol Cell 18:66-75. 2007..Together, two rho kinases contribute to fibronectin matrix assembly in a different manner and cortical myosin II-driven contractility, but not stress fibers, may be critical in this activity...
- PKCbeta-dependent activation of RhoA by syndecan-4 during focal adhesion formationAthanassios Dovas
Division of Biomedical Sciences, Imperial College London, London, SW7 2AZ, UK
J Cell Sci 119:2837-46. 2006..All these data point to a mechanism whereby syndecan-4 signals to RhoA in a PKCalpha-dependent manner and PKCalpha directly influences RhoA activity...
- The CMV early enhancer/chicken beta actin (CAG) promoter can be used to drive transgene expression during the differentiation of murine embryonic stem cells into vascular progenitorsAnnika N Alexopoulou
National Heart and Lung Institute, Sir Alexander Fleming Building, Faculty of Medicine, Imperial College London SW7 2AZ, UK
BMC Cell Biol 9:2. 2008..Embryonic stem cells are difficult to transfect, while downregulation of promoter activity upon selection of stable transfectants has been reported, rendering the study of proteins by overexpression difficult...
- Cellular adhesion responses to the heparin-binding (HepII) domain of fibronectin require heparan sulfate with specific propertiesYashithra Mahalingam
Division of Biomedical Sciences, Imperial College London, London SW7 2AZ, United Kingdom
J Biol Chem 282:3221-30. 2007..Several separate, specific properties of cell surface HS are therefore required in cell adhesion responses to the fibronectin HepII domain...
- Regulation of cytoskeletal organization by syndecan transmembrane proteoglycansAtsuko Yoneda
Division of Biomedical Sciences, Faculty of Medicine, Imperial College London, Sir Alexander Fleming Building, Exhibition Road, UK
Matrix Biol 22:25-33. 2003..Here we will review current understanding of signaling through syndecans in cytoskeletal organization...
- The Rho kinases I and II regulate different aspects of myosin II activityAtsuko Yoneda
Division of Biomedical Sciences, Faculty of Medicine, Imperial College London, London SW7 2AZ, England, UK
J Cell Biol 170:443-53. 2005..ROCK II bound phosphatidylinositol 3,4,5P(3) and was sensitive to its levels, properties not shared by ROCK I. Therefore, endogenous ROCKs are distinctly regulated and in turn are involved with different myosin compartments...
- A conserved NXIP motif is required for cell adhesion properties of the syndecan-4 ectodomainJames R Whiteford
Biomedical Sciences Division, Faculty of Natural Sciences, Imperial College London, Sir Alexander Fleming Building, Exhibition Road, London SW7 2AZ, United Kingdom
J Biol Chem 281:32156-63. 2006..We have shown that cell adhesion to the syndecan-4 ectodomain involves beta1 integrins in several cell types...
- Syndecans-2 and -4; close cousins, but not identical twinsEok Soo Oh
Division of Biomedical Sciences, Imperial College London, Exhibition Road, London SW7 2AZ, United Kingdom
Mol Cells 17:181-7. 2004....
- Syndecans in wound healing, inflammation and vascular biologyAnnika N Alexopoulou
Division of Biomedical Sciences, Imperial College London, Sir Alexander Fleming Building, Exhibition Road, London SW7 2AZ, UK
Int J Biochem Cell Biol 39:505-28. 2007....
- Two distinct sites in sonic Hedgehog combine for heparan sulfate interactions and cell signaling functionsShu Chun Chang
Molecular Medicine Section, National Heart and Lung Institute, Imperial College London, South Kensington, London SW7 2AZ, United Kingdom
J Biol Chem 286:44391-402. 2011..These studies provide a new insight into the functional roles of hShh interactions with HSPGs, which may allow targeting this aspect of hShh biology in, for example, pancreatic ductal adenocarcinoma...
- RhoGDI: multiple functions in the regulation of Rho family GTPase activitiesAthanassios Dovas
Division of Biomedical Sciences, Imperial College London, Exhibition Road, London SW7 2AZ, UK
Biochem J 390:1-9. 2005..All these results point to a wider role for RhoGDI than initially perceived, making it a binding partner that can tightly control Rho GTPases, but which also allows them to reach their full spectrum of activities...
- Heparan sulfate regulates fibrillin-1 N- and C-terminal interactionsStuart A Cain
Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, University of Manchester, United Kingdom
J Biol Chem 283:27017-27. 2008..These data highlight how heparin/heparan sulfate controls fibrillin-1 interactions...
- Structural basis of syndecan-4 phosphorylation as a molecular switch to regulate signalingBon Kyung Koo
Department of Biochemistry and Protein Network Research Center, College of Science, Yonsei University, Seoul 120 749, South Korea
J Mol Biol 355:651-63. 2006..We conclude that the phosphorylation (Ser183) of syndecan-4 can play a critical role as a molecular switch to regulate its functions through conformational change...
- Hierarchy of ADAM12 binding to integrins in tumor cellsCharles Kumar Thodeti
Institute of Molecular Pathology, University of Copenhagen, Frederik V s vej 11, DK 2100 Copenhagen, Denmark
Exp Cell Res 309:438-50. 2005..We demonstrated that phosphoinositide-3-kinase appears to be central in regulating alpha9beta1 integrin cell spreading activity in response to ADAM12...
- Localization of the transmembrane proteoglycan syndecan-4 and its regulatory kinases in costameres of rat cardiomyocytes: a deconvolution microscopic studyW Barry VanWinkle
Department of Pathology and Laboratory Medicine, University of Texas Medical School at Houston, Houston, Texas 77030, USA
Anat Rec 268:38-46. 2002....
- Regulation of inositol phospholipid binding and signaling through syndecan-4John R Couchman
Department of Cell Biology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
J Biol Chem 277:49296-303. 2002..This is consistent with the codistribution of syndecan-4 with the alpha isoform of protein kinase C in focal adhesions...
- Syndecan-4 associates with alpha-actininDaniel K Greene
Department of Cell Biology, University of Alabama, Birmingham, Alabama 35294 0006, USA
J Biol Chem 278:7617-23. 2003..We now demonstrate by Triton X-100 extraction immunoprecipitation and in vitro binding assays that the focal adhesion component alpha-actinin interacts with syndecan-4 in a beta-integrin-independent manner...
- ADAM12/syndecan-4 signaling promotes beta 1 integrin-dependent cell spreading through protein kinase Calpha and RhoACharles Kumar Thodeti
Institute of Molecular Pathology, University of Copenhagen, Frederik V s vej 11, DK 2100, Copenhagen, Denmark
J Biol Chem 278:9576-84. 2003..In summary, these data provide novel insights into syndecan-4 signaling. Syndecan-4 can promote cell spreading in a beta(1) integrin-dependent fashion through PKCalpha and RhoA, and PKCalpha and RhoA likely function in separate pathways...
- Molecular characterization of chicken syndecan-2 proteoglycanLigong Chen
Department of Cell Biology and Cell Adhesion and Matrix Research Center, University of Alabama at Birmingham, Birmingham, AL 35294, USA
Biochem J 366:481-90. 2002..This pattern was different from that reported for syndecan-4, but consistent with the roles of syndecan-2 in neural maturation and in mesenchymal-matrix interactions...
- Direct binding of syndecan-4 cytoplasmic domain to the catalytic domain of protein kinase C alpha (PKC alpha) increases focal adhesion localization of PKC alphaSsang Taek Lim
Department of Cell Biology, University of Alabama at Birmingham, 35294, USA
J Biol Chem 278:13795-802. 2003..The data support a mechanism where syndecan-4 binds PKC alpha and localizes it to focal adhesions, whose assembly may be regulated by the kinase...
- Fibroblast invasive migration into fibronectin/fibrin gels requires a previously uncharacterized dermatan sulfate-CD44 proteoglycanRichard A F Clark
Department of Dermatology, School of Medicine, SUNY at Stony Brook, New York, 11794 8165, USA
J Invest Dermatol 122:266-77. 2004....
- Essential contribution of tumor-derived perlecan to epidermal tumor growth and angiogenesisXinnong Jiang
Department of Cell Biology, University of Alabama at Birmingham, Birmingham, AL, USA
J Histochem Cytochem 52:1575-90. 2004....
- Palmitoylation at Cys574 is essential for MT1-MMP to promote cell migrationNarayanapanicker Anilkumar
Department of Matrix Biology, Kennedy Institute of Rheumatology Division, Imperial College London, London, UK
FASEB J 19:1326-8. 2005..Taken together, palmitoylation of MT1-MMP is one of the key posttranslational modifications that determines MT1-MMP-dependent cell migration...
- Cell adhesion to fibrillin-1: identification of an Arg-Gly-Asp-dependent synergy region and a heparin-binding site that regulates focal adhesion formationDaniel V Bax
UK Centre for Tissue Engineering, University of Manchester, Manchester, M13 9PT, UK
J Cell Sci 120:1383-92. 2007..These integrin and syndecan adhesion motifs juxtaposed on fibrillin-1 are evolutionarily conserved and reminiscent of similar functional elements on fibronectin, highlighting their crucial functional importance...