Research Topics
| E W AltonSummaryAffiliation: Imperial College Country: UK Publications
| Collaborators
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Detail Information
Publications
Gene therapy for cystic fibrosisE Alton
National Heart and Lung Institute, Imperial College, Ion Transport Unit, Manresa Road, London SW3 6LR, UK
Expert Opin Investig Drugs 9:1523-35. 2000..Current research is now focusing more on the barriers faced by delivery agents, with the aim that more efficient gene delivery will lead to a gene therapeutic for cystic fibrosis...- Cationic lipid-mediated CFTR gene transfer to the lungs and nose of patients with cystic fibrosis: a double-blind placebo-controlled trialE W Alton
Department of Gene Therapy, Imperial College at National Heart and Lung Institute, London, UK
Lancet 353:947-54. 1999..We studied the safety and efficacy of this gene transfer to the lungs and nose of patients with cystic fibrosis in a double-blind placebo-controlled trial... - Inflammation in cystic fibrosis airways: relationship to increased bacterial adherenceP Scheid
Dept. of Gene Therapy, Imperial College at the National Heart and Lung Institute, London, UK
Eur Respir J 17:27-35. 2001..The authors conclude that the stimulus produced by Pseudomonas aeruginosa is the predominant inflammatory trigger in their models... - Bioelectric characteristics of exon 10 insertional cystic fibrosis mouse: comparison with humansS N Smith
Ion Transport Unit, National Heart and Lung Institute, London, United Kingdom
Am J Physiol 268:C297-307. 1995..We conclude that the majority of the salient electrophysiological features of CF required for studies of pathogenesis or testing of new treatments are present in these cf/cf mice... - Effect of IBMX and alkaline phosphatase inhibitors on Cl- secretion in G551D cystic fibrosis mutant miceS N Smith
Ion Transport Unit, National Heart and Lung Institute, London, United Kingdom
Am J Physiol 274:C492-9. 1998..We conclude that IBMX is able to induce detectable levels of CFTR-related Cl- secretion in the intestinal tract but not the respiratory tract through the G551D mutant protein... 
Second-messenger regulation of sodium transport in mammalian airway epitheliaA Graham
Ion Transport Laboratory, National Heart and Lung Institute, London
J Physiol 453:475-91. 1992..Neither effect could be abolished by amiloride pretreatment. In human bronchi, a small decrease in ISC which could not be distinguished from that occurring in controls was observed.(ABSTRACT TRUNCATED AT 400 WORDS)..- In vivo transfer of bacterial marker genes results in differing levels of gene expression and tumor progression in immunocompetent and immunodeficient miceK V Lukacs
National Heart and Lung Institute at Imperial College, London, UK
Hum Gene Ther 10:2373-9. 1999.... - Mucus altering agents as adjuncts for nonviral gene transfer to airway epitheliumS Ferrari
Department of Gene Therapy, Imperial College at the National Heart and Lung Institute, London, UK
Gene Ther 8:1380-6. 2001..These results, whilst unlikely to be sufficient in themselves to achieve clinically relevant gene therapy, may be a further useful step in the attainment of this goal... - Gene therapy for cystic fibrosisJ C Davies
Department of Gene Therapy, Imperial College at the National Heart and Lung Institute, London, UK
J Gene Med 3:409-17. 2001..We highlight the problems encountered, and likely future directions of the field... - Non-invasive liposome-mediated gene delivery can correct the ion transport defect in cystic fibrosis mutant miceE W Alton
Ion Transport Laboratory, National Heart and Lung Institute, London, UK
Nat Genet 5:135-42. 1993..The non-viral nature and potentially lower immunogenicity of DNA-liposomes suggest that this may offer a therapeutic alternative to adenoviral therapies... - Recent progress in gene therapy for cystic fibrosisU Griesenbach
Department of Gene Therapy, Imperial College School of Medicine, National Heart and Lung Institute, London, UK
Curr Opin Mol Ther 3:385-9. 2001..Here, we review the progress in CF gene therapy over the last 12 months, including recent advances in viral and non-viral gene transfer agents and novel strategies, such as RNA repair and stem cell gene therapy... - Taking stock of gene therapy for cystic fibrosisM Stern
Department of Gene Therapy, Imperial College at the National Heart and Lung Institute, London, UK
Respir Res 1:78-81. 2000..Gene therapy for CF remains the most promising possibility for curative rather than symptomatic therapy... - Prospects for gene therapy in lung diseaseJ C Davies
Imperial College at the National Heart and Lung Institute, London, UK
Curr Opin Pharmacol 1:272-7. 2001..Recently, progress has been made in both laboratory and clinical studies of gene therapy for cystic fibrosis, alpha1-antitrypsin deficiency and lung cancer... - Localization and up-regulation of mucin (MUC2) gene expression in human nasal biopsies of patients with cystic fibrosisD Li
Lung Pathology Unit, National Heart and Lung Institute, London, U K
J Pathol 181:305-10. 1997..It is concluded that the MUC2 gene is expressed at three- to four-fold higher levels in CF nasal mucosa than in non-CF nasal tissue and that it is expressed in a variety of cells additional to submucosal mucus-secreting glands... - Effect of inhaled furosemide on metabisulfite- and methacholine-induced bronchoconstriction and nasal potential difference in asthmatic subjectsG M Nichol
Department of Thoracic Medicine, National Heart and Lung Institute, London, United Kingdom
Am Rev Respir Dis 142:576-80. 1990..Nasal PD was measured before and after placebo or furosemide, and again after MBS inhalation.(ABSTRACT TRUNCATED AT 250 WORDS)..