Nicola J Hewitt

Summary

Country: UK

Publications

  1. ncbi request reprint Cryopreserved rat, dog and monkey hepatocytes: measurement of drug metabolizing enzymes in suspensions and cultures
    Nicola J Hewitt
    In Vitro Technologies Inc, 1450 South Rolling Road, Baltimore, MD 21227, USA
    Hum Exp Toxicol 23:307-16. 2004
  2. pmc Recent advances in 2D and 3D in vitro systems using primary hepatocytes, alternative hepatocyte sources and non-parenchymal liver cells and their use in investigating mechanisms of hepatotoxicity, cell signaling and ADME
    Patricio Godoy
    Leibniz Research Centre for Working Environment and Human Factors IfADo, 44139, Dortmund, Germany
    Arch Toxicol 87:1315-530. 2013
  3. doi request reprint Use of human in vitro skin models for accurate and ethical risk assessment: metabolic considerations
    Nicola J Hewitt
    SWS, 64390 Erzhausen, Germany
    Toxicol Sci 133:209-17. 2013
  4. doi request reprint Optimisation of the cryopreservation of primary hepatocytes
    Nicola J Hewitt
    Scientific Writing Services, Erzhausen, Germany
    Methods Mol Biol 640:83-105. 2010
  5. ncbi request reprint Primary hepatocytes: current understanding of the regulation of metabolic enzymes and transporter proteins, and pharmaceutical practice for the use of hepatocytes in metabolism, enzyme induction, transporter, clearance, and hepatotoxicity studies
    Nicola J Hewitt
    Scientific Writing Services, Wingertstrasse, Erzhausen, Germany
    Drug Metab Rev 39:159-234. 2007
  6. ncbi request reprint Induction of drug metabolizing enzymes: a survey of in vitro methodologies and interpretations used in the pharmaceutical industry--do they comply with FDA recommendations?
    Nicola J Hewitt
    Chem Biol Interact 168:51-65. 2007
  7. ncbi request reprint Prediction of hepatic clearance using cryopreserved human hepatocytes: a comparison of serum and serum-free incubations
    Nadège Blanchard
    F Hoffmann La Roche AG, Pharmaceuticals Division, CH 4070 Basel, Switzerland
    J Pharm Pharmacol 58:633-41. 2006
  8. ncbi request reprint The effect of EGF and the comitogen, norepinephrine, on the proliferative responses of fresh and cryopreserved rat and mouse hepatocytes
    Julie A Price
    In Vitro Technologies Inc 1450 South Rolling Road, Baltimore, MD 21227, USA
    Cryobiology 53:182-93. 2006
  9. ncbi request reprint Correlation between troglitazone cytotoxicity and drug metabolic enzyme activities in cryopreserved human hepatocytes
    Nicola J Hewitt
    In Vitro Technologies, Inc, 1450 South Rolling Road, Baltimore, MD 21227, USA
    Chem Biol Interact 142:73-82. 2002
  10. ncbi request reprint Differential in vitro hepatotoxicity of troglitazone and rosiglitazone among cryopreserved human hepatocytes from 37 donors
    Scott Lloyd
    In Vitro Technologies, Inc, 1450 South Rolling Road, Baltimore, MD 21227, USA
    Chem Biol Interact 142:57-71. 2002

Collaborators

  • Stefan Pfuhler
  • Pierre Aeby
  • Ellen Fritsche
  • Robert J Edwards
  • J Brian Houston
  • Albert P Li
  • Christian Skonberg
  • Julia Scheel
  • Andre Guillouzo
  • Scott Lloyd
  • Patricio Godoy
  • Julie A Price
  • Nadège Blanchard
  • Michael Schwarz
  • Uta Dahmen
  • Jian Dong
  • Thomas S Weiss
  • Peter Olinga
  • Stefan Hoehme
  • Chris E P Goldring
  • Cliff Rowe
  • Joanna Fraczek
  • Jennifer Luebke-Wheeler
  • Patrick McMullen
  • Jennifer Bolleyn
  • Francesco Pampaloni
  • Anup Ramachandran
  • Olaf Dirsch
  • Agata Widera
  • Daniel J Maltman
  • Jessica Mwinyi
  • Valentina Fonsato
  • Geny M M Groothuis
  • Maria Teresa Donato
  • Andreas K Nussler
  • Yuichi Sugiyama
  • Melvin E Andersen
  • Jan G Hengstler
  • Jens Hrach
  • Neil R Cameron
  • Regina Stöber
  • Wolfgang E Thasler
  • Adam Hayward
  • Ute Albrecht
  • Tamara Vanhaecke
  • Karine Sá Ferreira
  • Courtney G Woods
  • Stefan A Przyborski
  • Hermann Georg Holzhütter
  • Claus Hellerbrand
  • Mathieu Vinken
  • Kathrin Schmich
  • Jens M Kelm
  • Rowena Eakins
  • Celine Schelcher
  • Sudin Bhattacharya
  • Claus Kordes
  • Kiyomi Ito
  • Gerd A Kullak-Ublick
  • Irmgard Merfort
  • Kathy M Yarborough
  • Yun Jaie Choi
  • Andrew Gibson
  • Johannes Georg Bode
  • Vera Rogiers
  • María José Gómez-Lechón
  • Simon Messner
  • Ciro Tetta
  • Linda Pluta
  • Peng Lu
  • Jinghai James Xu
  • Christoph Borner
  • Madlen Matz-Soja
  • Dean J Naisbitt
  • Philip Hewitt
  • Rolf Gebhardt
  • Robert A Budinsky
  • Dirk Drasdo
  • Hartmut Jaeschke
  • Nariman Ansari
  • Christoph Meyer
  • B Kevin Park
  • Anna Lutz
  • Georg Damm
  • Britta Burkhardt
  • Seddik Hammad
  • David Hallifax
  • Ernst H K Stelzer
  • Edward L LeCluyse
  • Bruno Stieger

Detail Information

Publications10

  1. ncbi request reprint Cryopreserved rat, dog and monkey hepatocytes: measurement of drug metabolizing enzymes in suspensions and cultures
    Nicola J Hewitt
    In Vitro Technologies Inc, 1450 South Rolling Road, Baltimore, MD 21227, USA
    Hum Exp Toxicol 23:307-16. 2004
    ..In conclusion, these data suggest that the enzyme characteristics of fresh and CP hepatocytes from each species and under specific incubation conditions should be considered when carrying out metabolism studies of new compounds...
  2. pmc Recent advances in 2D and 3D in vitro systems using primary hepatocytes, alternative hepatocyte sources and non-parenchymal liver cells and their use in investigating mechanisms of hepatotoxicity, cell signaling and ADME
    Patricio Godoy
    Leibniz Research Centre for Working Environment and Human Factors IfADo, 44139, Dortmund, Germany
    Arch Toxicol 87:1315-530. 2013
    ..One key message is that despite our enthusiasm for in vitro systems, we must never lose sight of the in vivo situation. Although hepatocytes have been isolated for decades, the hunt for relevant alternative systems has only just begun...
  3. doi request reprint Use of human in vitro skin models for accurate and ethical risk assessment: metabolic considerations
    Nicola J Hewitt
    SWS, 64390 Erzhausen, Germany
    Toxicol Sci 133:209-17. 2013
    ..These projects have enabled a better understanding of the contribution of XMEs to toxicity endpoints...
  4. doi request reprint Optimisation of the cryopreservation of primary hepatocytes
    Nicola J Hewitt
    Scientific Writing Services, Erzhausen, Germany
    Methods Mol Biol 640:83-105. 2010
    ..Special emphasis is placed on human hepatocytes but information for the cryopreservation of animal hepatocytes is also described. Finally, a suggested method for optimising cryopreservation method is outlined...
  5. ncbi request reprint Primary hepatocytes: current understanding of the regulation of metabolic enzymes and transporter proteins, and pharmaceutical practice for the use of hepatocytes in metabolism, enzyme induction, transporter, clearance, and hepatotoxicity studies
    Nicola J Hewitt
    Scientific Writing Services, Wingertstrasse, Erzhausen, Germany
    Drug Metab Rev 39:159-234. 2007
    ..We also report pharmaceutical perspectives of these topics and compare methods and interpretations for the drug development process...
  6. ncbi request reprint Induction of drug metabolizing enzymes: a survey of in vitro methodologies and interpretations used in the pharmaceutical industry--do they comply with FDA recommendations?
    Nicola J Hewitt
    Chem Biol Interact 168:51-65. 2007
    ..Although the two-fold induction criterion is now obsolete, more predictive models for determining the clinical induction potential are needed. Alternative models are proposed and discussed herein...
  7. ncbi request reprint Prediction of hepatic clearance using cryopreserved human hepatocytes: a comparison of serum and serum-free incubations
    Nadège Blanchard
    F Hoffmann La Roche AG, Pharmaceuticals Division, CH 4070 Basel, Switzerland
    J Pharm Pharmacol 58:633-41. 2006
    ..In the presence of serum, 85% of the compounds were successfully predicted within a 2-fold error. In conclusion, cryopreserved human hepatocyte suspensions represented a convenient and predictive model to assess human drug clearance...
  8. ncbi request reprint The effect of EGF and the comitogen, norepinephrine, on the proliferative responses of fresh and cryopreserved rat and mouse hepatocytes
    Julie A Price
    In Vitro Technologies Inc 1450 South Rolling Road, Baltimore, MD 21227, USA
    Cryobiology 53:182-93. 2006
    ..The secondary mitogenic effect of NE is also retained by cryopreserved rat hepatocytes, suggesting that these cells retain alpha1-receptor function...
  9. ncbi request reprint Correlation between troglitazone cytotoxicity and drug metabolic enzyme activities in cryopreserved human hepatocytes
    Nicola J Hewitt
    In Vitro Technologies, Inc, 1450 South Rolling Road, Baltimore, MD 21227, USA
    Chem Biol Interact 142:73-82. 2002
    ..53). Based on the correlation, we hypothesize that TRO and TRO sulfate are direct acting toxicants, whereas CYP3A4 oxidation and glucuronidation are detoxification pathways...
  10. ncbi request reprint Differential in vitro hepatotoxicity of troglitazone and rosiglitazone among cryopreserved human hepatocytes from 37 donors
    Scott Lloyd
    In Vitro Technologies, Inc, 1450 South Rolling Road, Baltimore, MD 21227, USA
    Chem Biol Interact 142:57-71. 2002
    ..We conclude that troglitazone and rosiglitazone are differentially toxic to human hepatocytes, and that toxicity may be independent of age, sex, tobacco use, and alcohol use...