Research Topics
| James S RobertsonSummaryAffiliation: Health Protection Agency Country: UK Publications
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Detail Information
Publications
The development of vaccine viruses against pandemic A(H1N1) influenzaJames S Robertson
Division of Virology, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar EN6 3QG, UK
Vaccine 29:1836-43. 2011..Two approaches were followed, the classical reassortment approach and the more recent reverse genetics approach. This report describes the development and the characteristics of current pandemic H1N1 candidate vaccine viruses...- Prospects for controlling future pandemics of influenzaJames S Robertson
National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, EN6 3QG, UK
Virus Res 162:39-46. 2011..There needs to be a continued focus on improvement to the vaccine response system that will require close collaboration between influenza and vaccine experts, manufacturers, regulators and public health authorities around the world... 
Permissible variation in the 3' non-coding region of the haemagglutinin genome segment of the H5N1 candidate influenza vaccine virus NIBRG-14 [corrected]Rachel E Johnson
Division of Virology, National Institute for Biological Standards and Control, Health Protection Agency, Blanche Lane, Potters Bar, Hertfordshire, United Kingdom
PLoS ONE 7:e36241. 2012..These findings may serve to assure the influenza vaccine community that generation of CVVs using best-guess NCR sequences, based on sequence alignments, are likely to produce robust viruses...- An additional oligosaccharide moiety in the HA of a pandemic influenza H1N1 candidate vaccine virus confers increased antigen yield in eggsCarolyn Nicolson
Division of Virology, National Institute for Biological Standards and Control, South Mimms, Potters Bar, Hertfordshire, EN6 3QG, UK
Vaccine 30:745-51. 2012..However, position 119 may be a useful locus to monitor in future for viruses and CVVs with potentially high yield... - Improved antigen yield in pandemic H1N1 (2009) candidate vaccine viruses with chimeric hemagglutinin moleculesRuth Harvey
Division of Virology, National Institute for Biological Standards and Control, Health Protection Agency, Blanche Lane, Potters Bar, Hertfordshire EN6 3QG, United Kingdom
J Virol 85:6086-90. 2011..Here we show that these new viruses have considerably improved growth in eggs and are therefore better candidate vaccine viruses for use in production of pandemic H1N1 (2009) vaccine... - Current challenges in implementing cell-derived influenza vaccines: implications for production and regulation, July 2007, NIBSC, Potters Bar, UKPhilip D Minor
National Institute for Biological Standards and Control, South Mimms, Potters Bar, Herts, UK
Vaccine 27:2907-13. 2009..Future studies to progress the field were reviewed... - Improved haemagglutinin antigen content in H5N1 candidate vaccine viruses with chimeric haemagglutinin moleculesRuth Harvey
Division of Virology, National Institute for Biological Standards and Control, Health Protection Agency, Blanche Lane, Potters Bar, Hertfordshire EN6 3QG, United Kingdom
Vaccine 28:8008-14. 2010..Here we show that a number of these viruses have improved HA antigen content and yield and are therefore better candidate vaccine viruses for use in production of H5N1 vaccine... - Application of deglycosylation to SDS PAGE analysis improves calibration of influenza antigen standardsRuth Harvey
Division of Virology, National Institute for Biological Standards and Control, Health Protection Agency, Blanche Lane, South Mimms, Potters Bar, Hertfordshire EN6 3QG, United Kingdom
Biologicals 40:96-9. 2012..We found that while the final agreed HA value of the samples tested was not significantly different with or without deglycosylation, the deglycosylation step greatly improved between-laboratory agreement... - Quantitation of haemagglutinin in H5N1 influenza viruses reveals low haemagglutinin content of vaccine virus NIBRG-14 (H5N1)Ruth Harvey
Division of Virology, National Institute for Biological Standards and Control, Blanche Lane, Potters Bar, Hertfordshire EN6 3QG, United Kingdom
Vaccine 26:6550-4. 2008..This was also found to be the case for the parent wild type A/Viet Nam/1194/2004 virus... 
Generation of influenza vaccine viruses on Vero cells by reverse genetics: an H5N1 candidate vaccine strain produced under a quality systemCarolyn Nicolson
Division of Virology, National Institute for Biological Standards and Control, South Mimms, Potters Bar, Herts EN6 3QG, UK
Vaccine 23:2943-52. 2005....- Assuring the quality, safety, and efficacy of DNA vaccinesJames S Robertson
National Institute for Biological Standards and Control, Herts, UK
Methods Mol Med 127:363-74. 2006.... - Reference viruses for seasonal and pandemic influenza vaccine preparationJohn S Wood
Division of Virology, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, Hertfordshire, UK
Influenza Other Respi Viruses 1:5-9. 2007..However, it is not possible to use such approaches in deriving vaccine reference viruses from highly pathogenic H5N1 viruses and alternative techniques such as reverse genetics must be employed... - Developing vaccines to combat pandemic influenzaJames S Robertson
Division of Virology, National Institute for Biological Standards and Control, Health Protection Agency, Blanche Lane, South Mimms, Potters Bar, EN6 3QG, UK E Mail O G E
Viruses 2:532-46. 2010..Reverse genetics has also been used successfully alongside classical reassorting techniques in the development of (swine flu) pandemic A(H1N1)v vaccine viruses...