D J Flint

Summary

Affiliation: Hannah Research Institute
Country: UK

Publications

  1. ncbi request reprint Diet-induced obesity impairs mammary development and lactogenesis in murine mammary gland
    David J Flint
    Hannah Research Institute, Ayr KA6 5HL, UK
    Am J Physiol Endocrinol Metab 288:E1179-87. 2005
  2. pmc Insulin-like growth factor-binding protein-5 (IGFBP-5): a critical member of the IGF axis
    James Beattie
    Hannah Research Institute, Ayr KA6 5HL, Scotland, UK
    Biochem J 395:1-19. 2006
  3. ncbi request reprint Molecular interactions in the insulin-like growth factor (IGF) axis: a surface plasmon resonance (SPR) based biosensor study
    James Beattie
    Strathclyde Institute of Pharmacy and Biomedical Science, Royal College Building, University of Strathclyde, Glasgow, UK
    Mol Cell Biochem 307:221-36. 2008
  4. ncbi request reprint Modulation of the actions of IGFs by IGFBP-5 in the mammary gland
    D J Flint
    Hannah Research Institute, Ayr, UK
    Horm Metab Res 35:809-15. 2003
  5. ncbi request reprint Insulin-like growth factor binding proteins initiate cell death and extracellular matrix remodeling in the mammary gland
    D J Flint
    Hannah Research Institute, Ayr KA6 5HL, UK
    Domest Anim Endocrinol 29:274-82. 2005
  6. ncbi request reprint Prolactin inhibits cell loss and decreases matrix metalloproteinase expression in the involuting mouse mammary gland but fails to prevent cell loss in the mammary glands of mice expressing IGFBP-5 as a mammary transgene
    D J Flint
    Hannah Research Institute, Ayr KA6 5HL, UK
    J Mol Endocrinol 36:435-48. 2006
  7. ncbi request reprint Hormonal control of IGF-binding protein (IGFBP)-5 and IGFBP-2 secretion during differentiation of the HC11 mouse mammary epithelial cell line
    K Phillips
    Molecular Recognition Group, Hannah Research Institute, Ayr KA6 5HL, UK
    J Mol Endocrinol 31:197-208. 2003
  8. ncbi request reprint Insulin-like growth factor binding proteins: IGF-dependent and -independent effects in the mammary gland
    D J Flint
    Hannah Research Institute, Ayr, United Kingdom
    J Mammary Gland Biol Neoplasia 5:65-73. 2000
  9. ncbi request reprint Generation of epitope-specific antibodies to rat GHBP in the sheep using an interspecies switching strategy involving site-directed mutagenesis of ovine GHBP
    J H Shand
    Hannah Research Institute, Ayr, UK
    Eur J Biochem 266:917-23. 1999
  10. ncbi request reprint A quantitative RT-PCR study of the mRNA expression profile of the IGF axis during mammary gland development
    M Boutinaud
    Molecular Recognition Group, Hannah Research Institute, Ayr KA6 5HL, UK
    J Mol Endocrinol 33:195-207. 2004

Collaborators

Detail Information

Publications34

  1. ncbi request reprint Diet-induced obesity impairs mammary development and lactogenesis in murine mammary gland
    David J Flint
    Hannah Research Institute, Ayr KA6 5HL, UK
    Am J Physiol Endocrinol Metab 288:E1179-87. 2005
    ..Obese animals also exhibited marked abnormalities in alveolar development of the mammary gland, which may partially explain the delay in differentiation evident during lactogenesis...
  2. pmc Insulin-like growth factor-binding protein-5 (IGFBP-5): a critical member of the IGF axis
    James Beattie
    Hannah Research Institute, Ayr KA6 5HL, Scotland, UK
    Biochem J 395:1-19. 2006
    ..We also examine the regulation of IGFBP-5 expression and comment on its potential role in tumour biology, with special reference to work with breast cancer cells...
  3. ncbi request reprint Molecular interactions in the insulin-like growth factor (IGF) axis: a surface plasmon resonance (SPR) based biosensor study
    James Beattie
    Strathclyde Institute of Pharmacy and Biomedical Science, Royal College Building, University of Strathclyde, Glasgow, UK
    Mol Cell Biochem 307:221-36. 2008
    ..We analyse the interaction of IGFBP-5 (and IGFBP-3) with heparin and other biomolecules and describe experiments, which were designed to monitor multi-protein complex formation in this molecular axis...
  4. ncbi request reprint Modulation of the actions of IGFs by IGFBP-5 in the mammary gland
    D J Flint
    Hannah Research Institute, Ayr, UK
    Horm Metab Res 35:809-15. 2003
    ....
  5. ncbi request reprint Insulin-like growth factor binding proteins initiate cell death and extracellular matrix remodeling in the mammary gland
    D J Flint
    Hannah Research Institute, Ayr KA6 5HL, UK
    Domest Anim Endocrinol 29:274-82. 2005
    ..The ability to separate these properties, using mutant IGFBPs, is currently under investigation...
  6. ncbi request reprint Prolactin inhibits cell loss and decreases matrix metalloproteinase expression in the involuting mouse mammary gland but fails to prevent cell loss in the mammary glands of mice expressing IGFBP-5 as a mammary transgene
    D J Flint
    Hannah Research Institute, Ayr KA6 5HL, UK
    J Mol Endocrinol 36:435-48. 2006
    ..PRL is a potent inhibitor, both of cell death, an effect which is suppressed by IGFBP-5, and of MMP expression, which is independent of the actions of IGFBP-5...
  7. ncbi request reprint Hormonal control of IGF-binding protein (IGFBP)-5 and IGFBP-2 secretion during differentiation of the HC11 mouse mammary epithelial cell line
    K Phillips
    Molecular Recognition Group, Hannah Research Institute, Ayr KA6 5HL, UK
    J Mol Endocrinol 31:197-208. 2003
    ....
  8. ncbi request reprint Insulin-like growth factor binding proteins: IGF-dependent and -independent effects in the mammary gland
    D J Flint
    Hannah Research Institute, Ayr, United Kingdom
    J Mammary Gland Biol Neoplasia 5:65-73. 2000
    ..We hope to be able to explore the role of this binding protein in the mammary gland with a transgenic mouse model expressing IGFBP-5 on the beta-lactoglobulin promoter...
  9. ncbi request reprint Generation of epitope-specific antibodies to rat GHBP in the sheep using an interspecies switching strategy involving site-directed mutagenesis of ovine GHBP
    J H Shand
    Hannah Research Institute, Ayr, UK
    Eur J Biochem 266:917-23. 1999
    ....
  10. ncbi request reprint A quantitative RT-PCR study of the mRNA expression profile of the IGF axis during mammary gland development
    M Boutinaud
    Molecular Recognition Group, Hannah Research Institute, Ayr KA6 5HL, UK
    J Mol Endocrinol 33:195-207. 2004
    ..Overall, this study has demonstrated the importance and complexity of the IGF axis during mammary gland development and provides a valuable resource for future research in this area...
  11. ncbi request reprint Effects of food restriction on the responses of the mammary gland and adipose tissue to prolactin and growth hormone in the lactating rat
    D J Flint
    Hannah Research Institute, Ayr, UK
    J Endocrinol 156:299-305. 1998
    ....
  12. ncbi request reprint Identification of novel sites in the ovine growth hormone receptor involved in binding hormone and conferring species specificity
    G J Allan
    Hannah Research Institute, Ayr, Scotland, UK
    Eur J Biochem 261:555-62. 1999
    ..4-fold increase in the affinity of binding to rat GH. Taken together, the changes in binding affinity of the site-B mutant for rat and bovine GH demonstrate that this site is involved in conferring species specificity for binding GH...
  13. ncbi request reprint Regional and interspecific differences in the ligand binding properties of beta-adrenergic receptors of individual white adipose tissue depots in the sheep and rat
    W P Bowen
    Hannah Research Institute, Ayr, U K
    Biochem Pharmacol 44:681-6. 1992
    ..The properties of the beta-adrenergic receptor binding site thus show species and depot specific differences...
  14. ncbi request reprint Insulin-like growth factor axis during embryonic development
    G J Allan
    Hannah Research Institute, Ayr KA6 5HL, UK
    Reproduction 122:31-9. 2001
    ..This article reviews the work that has been carried out in this area in the context of current understanding of the IGF system...
  15. ncbi request reprint The carboxy-terminal domain of IGF-binding protein-5 inhibits heparin binding to a site in the central domain
    H Song
    Hannah Research Institute, Ayr, UK
    J Mol Endocrinol 26:229-39. 2001
    ..In light of these findings, we suggest that C-terminally truncated fragments of IGFBP-5 generated in vivo by proteolysis could retain heparin/extracellular matrix binding properties...
  16. ncbi request reprint Control of milk secretion and apoptosis during mammary involution
    C J Wilde
    Hannah Research Institute, Ayr, United Kingdom
    J Mammary Gland Biol Neoplasia 4:129-36. 1999
    ..Local control of apoptosis in rodents during weaning, and after peak lactation in dairy animals, may be due to the actions of milk-borne survival factors or their inhibitors, and can be manipulated by frequency of milk removal...
  17. ncbi request reprint Effects of antibodies to adipocytes on body weight, food intake, and adipose tissue cellularity in obese rats
    D J Flint
    Hannah Research Institute, Ayr, KA6 5HL, United Kingdom
    Biochem Biophys Res Commun 252:263-8. 1998
    ..These results demonstrate for the first time, the potential to treat diet-induced obesity with antibodies to adipocytes by producing long-term reductions in the number of adipocytes, with minimal side-effects...
  18. ncbi request reprint The role of IGFBP-5 in mammary gland development and involution
    Gordon J Allan
    Hannah Research Institute, Ayr KA65HL, Scotland, UK
    Domest Anim Endocrinol 27:257-66. 2004
    ..Thus, IGFBP-5 seems important for regulating both apoptosis and cell proliferation in the mammary gland during development and post-lactation involution...
  19. ncbi request reprint Altered expression of insulin-like growth factor-1 and insulin like growth factor binding proteins-2 and 5 in the mouse mutant Hypodactyly (Hd) correlates with sites of apoptotic activity
    G J Allan
    Hannah Research Institute, Ayr, UK
    Anat Embryol (Berl) 202:1-11. 2000
    ..We propose that these three components of the IGF axis could be involved in the manifestation of the mutant phenotype in Hypodactyly, and that this is probably a result of their ability to regulate cell survival and cell death...
  20. ncbi request reprint Insulin-like growth factor binding protein (IGFBP)-5 is upregulated during both differentiation and apoptosis in primary cultures of mouse mammary epithelial cells
    Jennifer D Lochrie
    Molecular Recognition Group, Hannah Research Institute, Ayr, Scotland
    J Cell Physiol 207:471-9. 2006
    ..We discuss the possible implications of our observations in relation to the physiological processes of pregnancy, lactation, and involution in the mammary gland and the associated changes in mammary epithelial cell function...
  21. ncbi request reprint Regulation of genes encoding proteolytic enzymes during mammary gland development
    David A Sorrell
    Molecular Recognition Group, Hannah Research Institute, Ayr, UK
    J Dairy Res 72:433-41. 2005
    ..2003). A number of knock-out mice, which are deficient for individual MMP genes (e.g., MMP2, MMP3) or plasminogen, display alterations to mammary gland structure and impairment of lactation (Lund et al. 1999; Wiseman et al. 2003)...
  22. ncbi request reprint Molecular recognition characteristics in the insulin-like growth factor (IGF)-insulin-like growth factor binding protein -3/5 (IGFBP-3/5) heparin axis
    James Beattie
    Department of Molecular Recognition, Hannah Research Institute, Ayr KA6 5HL, Scotland
    J Mol Endocrinol 34:163-75. 2005
    ..The potential biological significance of our data is highlighted by the demonstration that IGF-I and IGF-II can displace endogenous IGFBP-5 from monolayer cultures of the mouse mammary epithelial cell line HC11...
  23. ncbi request reprint Insulin-like growth factor binding protein-5 (IGFBP-5) induces premature cell death in the mammary glands of transgenic mice
    Elizabeth Tonner
    Hannah Research Institute, Ayr KA6 5HL, England, UK
    Development 129:4547-57. 2002
    ..It clearly demonstrates that IGF-I is an important developmental/survival factor for the mammary gland and, furthermore, this cell death programme may be utilised in a wide variety of tissues...
  24. ncbi request reprint Metabolic safety-margins do not differ between cows of high and low genetic merit for milk production
    Christopher H Knight
    Hannah Research Institute, Ayr KA6 5HL, UK
    J Dairy Res 71:141-53. 2004
    ..The results do not support the commonly held belief that selective breeding of dairy cows for high milk production has rendered them markedly more susceptible to metabolic disturbances...
  25. ncbi request reprint Effects of growth hormone and prolactin on adipose tissue development and function
    David J Flint
    Hannah Research Institute, Ayr KA6 5HL, UK
    Pituitary 6:97-102. 2003
    ..However, recent studies suggest that this may be a physiological response in which adipose tissue sites containing lymphoid tissue (such as mesenteric) show preservation of adipose tissue perhaps to support augmented immune responses...
  26. ncbi request reprint Specific amino acid substitutions determine the differential contribution of the N- and C-terminal domains of insulin-like growth factor (IGF)-binding protein-5 in binding IGF-I
    John H Shand
    Hannah Research Institute, Ayr KA6 5HL, Scotland, United Kingdom
    J Biol Chem 278:17859-66. 2003
    ..Finally, although these residues lie within a major heparin-binding site in IGFBP-5 and -3, we also show that the mutations in C-Term have no effect on heparin binding...
  27. ncbi request reprint Major components of the insulin-like growth factor axis are expressed early in chicken embryogenesis, with IGF binding protein ( IGFBP) -5 expression subject to regulation by Sonic Hedgehog
    Gordon J Allan
    Hannah Research Institute, Ayr, Scotland, KA6 5HL, UK
    Anat Embryol (Berl) 207:73-84. 2003
    ....
  28. ncbi request reprint Cumulative mutagenesis of the basic residues in the 201-218 region of insulin-like growth factor (IGF)-binding protein-5 results in progressive loss of both IGF-I binding and inhibition of IGF-I biological action
    Gordon J Allan
    Hannah Research Institute, Ayr KA6 5HL, Scotland, United Kingdom
    Endocrinology 147:338-49. 2006
    ....
  29. ncbi request reprint Insulin-like growth factor-binding protein-5 activates plasminogen by interaction with tissue plasminogen activator, independently of its ability to bind to plasminogen activator inhibitor-1, insulin-like growth factor-I, or heparin
    Alice M Sorrell
    Hannah Research Institute, Ayr KA6 5HL, United Kingdom
    J Biol Chem 281:10883-9. 2006
    ..Thus IGFBP-5 can induce cell death by both sequestering IGF-I and enhancing plasmin generation...
  30. ncbi request reprint Growth hormone, acting in part through the insulin-like growth factor axis, rescues developmental, but not metabolic, activity in the mammary gland of mice expressing a single allele of the prolactin receptor
    Gordon J Allan
    Institut National de la Sante et de la Recherche Medicale, Unite 344, Faculte de Medecine Necker, 75730 Paris, France
    Endocrinology 143:4310-9. 2002
    ..This may be due to the maintenance by GH/IGF-I of a proliferative, rather than a differentiative, phenotype...
  31. ncbi request reprint Insulin-like growth factor binding protein 5 and apoptosis in mammary epithelial cells
    Emma Marshman
    School of Biological Sciences, Stopford Building, University of Manchester, Oxford Road, Manchester, M13 9PT, UK
    J Cell Sci 116:675-82. 2003
    ..This study provides evidence that IGFBP-5 plays an important role in the regulation of apoptosis in the mammary gland...
  32. ncbi request reprint Antibody-induced lysis of isolated rat epididymal adipocytes and complement activation in vivo
    Keith Dickinson
    Knoll Ltd, Nottingham, UK
    Obes Res 10:122-7. 2002
    ..To identify human monoclonal antibodies selectively binding to human adipocytes and to evaluate their ability to induce lysis of isolated rat adipocytes in vitro and to reduce rat complement levels in vivo...
  33. ncbi request reprint Sensitivity of hybrid ovine/rat GH receptors to oGH and rat GH in transfected FDC-P1 mouse myeloid cells in vitro
    James Beattie
    Department of Molecular Recognition, Hannah Research Institute, Ayr, Scotland
    Mol Cell Biochem 238:137-43. 2002
    ..We discuss these findings in the context of the relationships between affinity and activity at the GHR...
  34. ncbi request reprint Developmental aspects of adipose tissue in GH receptor and prolactin receptor gene disrupted mice: site-specific effects upon proliferation, differentiation and hormone sensitivity
    David J Flint
    Department of Bioscience, University of Strathclyde, Glasgow G1 1XW, UK
    J Endocrinol 191:101-11. 2006
    ..Our results provide in vivo evidence that both GH and PRL stimulate differentiation of adipocytes but that the effects of GH are site specific and induce intrinsic changes in the precursor population, which are retained in vitro...