Affiliation: Geneva Biomedical Research Institute
- Stereochemical course of enzymatic enolpyruvyl transfer and catalytic conformation of the active site revealed by the crystal structure of the fluorinated analogue of the reaction tetrahedral intermediate bound to the active site of the C115A mutant of MuT Skarzynski
Glaxo Wellcome Research and Development, Medicines Research Centre, Stevenage, Hertfordshire, United Kingdom SG1 2NY
Biochemistry 37:2572-7. 1998..J., and Walsh, C. T. (1995) J. Am. Chem. Soc. 117, 7329-7337], it now follows that the stereochemistry of elimination of H+ from C-3 and Pi from C-2 of the tetrahedral intermediate of the reaction is syn...
- Structure of UDP-N-acetylglucosamine enolpyruvyl transferase, an enzyme essential for the synthesis of bacterial peptidoglycan, complexed with substrate UDP-N-acetylglucosamine and the drug fosfomycinT Skarzynski
Glaxo Wellcome Research and Development, Medicines Research Centre, Stevenage, UK ts
Structure 4:1465-74. 1996..Inhibitors of enolpyruvyl transferases are of biotechnological interest as MurA and EPSP synthase are found exclusively in plants and microbes...
- The high-resolution crystal structure of a 24-kDa gyrase B fragment from E. coli complexed with one of the most potent coumarin inhibitors, clorobiocinF T Tsai
Laboratory of Molecular Biophysics, University of Oxford, United Kingdom
Proteins 28:41-52. 1997..Moreover, to understand the differences in energetics of binding of clorobiocin and novobiocin to the protein, the results from isothermal titration calorimetry are also presented...
- Structure of full-length porcine synovial collagenase reveals a C-terminal domain containing a calcium-linked, four-bladed beta-propellerJ Li
Blackert Laboratory, Imperial College, London, UK
Structure 3:541-9. 1995..This catalytic domain alone, which comprises about 150 amino acids, has no activity against collagen. A second domain, of 200 amino acids, is homologous to haemopexin, a haem-binding glycoprotein...
- The nature of inhibition of DNA gyrase by the coumarins and the cyclothialidines revealed by X-ray crystallographyR J Lewis
Laboratory of Molecular Biophysics, University of Oxford, UK
EMBO J 15:1412-20. 1996..These crystal structures consequently describe a chemically well characterized ligand binding surface and provide useful information to assist in the design of novel ligands...