Research Topics
| Simon A WellerSummaryAffiliation: Central Science Laboratory Country: UK Publications
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Publications
Identification of Agrobacterium spp. present within Brassica napus seed by TaqMan PCR--implications for GM screening proceduresSimon A Weller
Central Science Laboratory, DEFRA, Sand Hutton, York, YO41 1LZ, UK
Arch Microbiol 178:338-43. 2002..This is the first report of Agrobacterium sp. present within the seed of B. napus, a crop routinely screened for genetically modified DNA contamination using PCR assays with Agrobacterium sequences as targets...
Detection of Xanthomonas fragariae and presumptive detection of Xanthomonas arboricola pv. fragariae, from strawberry leaves, by real-time PCRS A Weller
Central Science Laboratory, DEFRA, Sand Hutton, York, YO41 1LZ, UK
J Microbiol Methods 70:379-83. 2007..arboricola pathovars. An existing genomic DNA extraction protocol was modified to facilitate detection of both pathogens to 10(3) cells per strawberry leaf disc...
Detection of Ralstonia solanacearum strains with a quantitative, multiplex, real-time, fluorogenic PCR (TaqMan) assayS A Weller
Central Science Laboratory, MAFF, Sand Hutton, York, YO41 1LZ, United Kingdom
Appl Environ Microbiol 66:2853-8. 2000..solanacearum...
Detection of root mat associated Agrobacterium strains from plant material and other sample types by post-enrichment TaqMan PCRS A Weller
Central Science Laboratory, DEFRA, York, UK
J Appl Microbiol 92:118-26. 2002..The development of a fluorogenic, 5' nuclease, TaqMan PCR assay for the detection of Ri-plasmids from root mat inducing Agrobacterium biovar 1 strains...
Acquisition of an Agrobacterium Ri plasmid and pathogenicity by other alpha-Proteobacteria in cucumber and tomato crops affected by root matS A Weller
Department of Environment, Food, and Rural Affairs, Sand Hutton, York YO41 1LZ
Appl Environ Microbiol 70:2779-85. 2004..radiobacter strains (64%). However, individual strains from these three genera all had transformation rates comparable to those observed from cotyledons inoculated with a rhizogenic Sinorhizobium strain (75%)...
Recurrent outbreaks of root mat in cucumber and tomato are associated with a monomorphic, cucumopine, Ri-plasmid harboured by various AlphaproteobacteriaSimon A Weller
Central Science Laboratory, DEFRA, Sand Hutton, York, UK
FEMS Microbiol Lett 258:136-43. 2006..Two other pRi, harboured by Agrobacterium isolated from a recent root mat outbreak in one tomato crop, were also shown to possess the GALLS gene but were shown not to be cucumopine pRi by PCR-RFLP...
