J McLauchlin

Summary

Affiliation: Central Public Health Laboratory
Country: UK

Publications

  1. ncbi Isolation and identification of Clostridium spp. from infections associated with the injection of drugs: experiences of a microbiological investigation team
    J S Brazier
    PHLS Anaerobe Reference Unit, University Hospital of Wales, Cardiff
    J Med Microbiol 51:985-9. 2002
  2. ncbi Amplified fragment length polymorphism (AFLP) analysis of Clostridium novyi, C. perfringens and Bacillus cereus isolated from injecting drug users during 2000
    J McLauchlin
    Division of Gastrointestinal Infections, PHLS Central Public Health Laboratory, London
    J Med Microbiol 51:990-1000. 2002
  3. ncbi An investigation into the microflora of heroin
    J McLauchlin
    Central Public Health Laboratory, Communicable Disease Surveillance Centre, London
    J Med Microbiol 51:1001-8. 2002
  4. pmc Molecular epidemiological analysis of Cryptosporidium spp. in the United Kingdom: results of genotyping Cryptosporidium spp. in 1,705 fecal samples from humans and 105 fecal samples from livestock animals
    J McLauchlin
    Food Safety Microbiology Laboratory, Division of Gastrointestinal Infections, PHLS Central Public Health Laboratory, London NW9 5HT, United Kingdom
    J Clin Microbiol 38:3984-90. 2000
  5. pmc Genetic characterization of Cryptosporidium strains from 218 patients with diarrhea diagnosed as having sporadic cryptosporidiosis
    J McLauchlin
    Food Hygiene Laboratory, Division of Gastrointestinal Infections, PHLS Central Public Health Laboratory, London NW9 5HT, United Kingdom
    J Clin Microbiol 37:3153-8. 1999
  6. ncbi An outbreak of serious illness and death among injecting drug users in England during 2000
    J A Jones
    Communicable Disease Surveillance Centre, Public Health Laboratory Service, London
    J Med Microbiol 51:978-84. 2002
  7. pmc Extraction and genotyping of Cryptosporidium parvum DNA from fecal smears on glass slides stained conventionally for direct microscope examination
    C Amar
    Food Safety Microbiology Laboratory, PHLS Division of Gastrointestinal Infections, Central Public Health Laboratory, London, NW9 5HT, SE18 8PF, United Kingdom
    J Clin Microbiol 39:401-3. 2001
  8. ncbi The epidemiology of cryptosporidiosis: application of experimental sub-typing and antibody detection systems to the investigation of water-borne outbreaks
    J McLauchlin
    Food Hygiene Laboratory, PHLS Central Public Health Laboratory, London, UK
    Folia Parasitol (Praha) 45:83-92. 1998
  9. ncbi The contamination of pâté with Listeria monocytogenes--results from the 1994 European Community-Coordinated Food Control Program for England and Wales
    G Nichols
    Environmental Surveillance Unit, Communicable Disease Surveillance Centre, London, UK
    J Food Prot 61:1299-304. 1998
  10. ncbi Unusual cryptosporidium species recovered from human faeces: first description of Cryptosporidium felis and Cryptosporidium 'dog type' from patients in England
    S Pedraza-Diaz
    Division of Gastrointestinal Infections, PHLS Central Public Health Laboratory, London
    J Med Microbiol 50:293-6. 2001

Collaborators

Detail Information

Publications35

  1. ncbi Isolation and identification of Clostridium spp. from infections associated with the injection of drugs: experiences of a microbiological investigation team
    J S Brazier
    PHLS Anaerobe Reference Unit, University Hospital of Wales, Cardiff
    J Med Microbiol 51:985-9. 2002
    ..Differentiation of C. novyi type A from C. botulinum type C in reference laboratories was based on 16S rDNA sequence data and specific neutralisation of cytopathic effects in tissue culture...
  2. ncbi Amplified fragment length polymorphism (AFLP) analysis of Clostridium novyi, C. perfringens and Bacillus cereus isolated from injecting drug users during 2000
    J McLauchlin
    Division of Gastrointestinal Infections, PHLS Central Public Health Laboratory, London
    J Med Microbiol 51:990-1000. 2002
    ..novyi type, or both, which either had an adaptive advantage in spore germination, survival or growth following the drug preparation and the injection procedure, or produced a more severe clinical presentation...
  3. ncbi An investigation into the microflora of heroin
    J McLauchlin
    Central Public Health Laboratory, Communicable Disease Surveillance Centre, London
    J Med Microbiol 51:1001-8. 2002
    ..subtilis alone and in one sample B. pumilus alone. C. botulinum and C. novyi were not isolated from any of the heroin samples. Recommendations for the optimal examination of the microflora of heroin are given...
  4. pmc Molecular epidemiological analysis of Cryptosporidium spp. in the United Kingdom: results of genotyping Cryptosporidium spp. in 1,705 fecal samples from humans and 105 fecal samples from livestock animals
    J McLauchlin
    Food Safety Microbiology Laboratory, Division of Gastrointestinal Infections, PHLS Central Public Health Laboratory, London NW9 5HT, United Kingdom
    J Clin Microbiol 38:3984-90. 2000
    ..The spring peak in cases was due to genotype 2. Genotype 1 was significantly more common in patients infected during the late-summer-autumn peak and in those with a history of foreign travel...
  5. pmc Genetic characterization of Cryptosporidium strains from 218 patients with diarrhea diagnosed as having sporadic cryptosporidiosis
    J McLauchlin
    Food Hygiene Laboratory, Division of Gastrointestinal Infections, PHLS Central Public Health Laboratory, London NW9 5HT, United Kingdom
    J Clin Microbiol 37:3153-8. 1999
    ..The distribution of the genotypes was significantly different both in patients with a history of foreign travel and in those from different regions in England...
  6. ncbi An outbreak of serious illness and death among injecting drug users in England during 2000
    J A Jones
    Communicable Disease Surveillance Centre, Public Health Laboratory Service, London
    J Med Microbiol 51:978-84. 2002
    ..Lessons learned have been used to produce guidance for the investigation and management of outbreaks of unexplained serious illness of possible infective aetiology...
  7. pmc Extraction and genotyping of Cryptosporidium parvum DNA from fecal smears on glass slides stained conventionally for direct microscope examination
    C Amar
    Food Safety Microbiology Laboratory, PHLS Division of Gastrointestinal Infections, Central Public Health Laboratory, London, NW9 5HT, SE18 8PF, United Kingdom
    J Clin Microbiol 39:401-3. 2001
    ..The correct genotype of Cryptosporidium parvum was amplified by PCR from 89 (85%) of 105 smears following conventional staining but not from negative controls. This technique may have applications for analysis of other infectious agents...
  8. ncbi The epidemiology of cryptosporidiosis: application of experimental sub-typing and antibody detection systems to the investigation of water-borne outbreaks
    J McLauchlin
    Food Hygiene Laboratory, PHLS Central Public Health Laboratory, London, UK
    Folia Parasitol (Praha) 45:83-92. 1998
    ..The significance of these findings is discussed in relation to data obtained from epidemiological field studies...
  9. ncbi The contamination of pâté with Listeria monocytogenes--results from the 1994 European Community-Coordinated Food Control Program for England and Wales
    G Nichols
    Environmental Surveillance Unit, Communicable Disease Surveillance Centre, London, UK
    J Food Prot 61:1299-304. 1998
    ..monocytogenes, and we suggest that the main areas of concern are cross-contamination and the length of display of pâté sold from opened packs...
  10. ncbi Unusual cryptosporidium species recovered from human faeces: first description of Cryptosporidium felis and Cryptosporidium 'dog type' from patients in England
    S Pedraza-Diaz
    Division of Gastrointestinal Infections, PHLS Central Public Health Laboratory, London
    J Med Microbiol 50:293-6. 2001
    ..The patient infected by C. dt had a recent history of travel to Africa. This is the first report of infection with these two Cryptosporidium species in immunocompetent patients, and in any patient in the UK...
  11. ncbi The detection of enterotoxins and toxic shock syndrome toxin genes in Staphylococcus aureus by polymerase chain reaction
    J McLauchlin
    Food Safety Microbiology Laboratory, PHLS Division of Gastrointestinal Infections, Central Public Health Laboratory, London, UK
    J Food Prot 63:479-88. 2000
    ..aureus: the appropriate SEA or SEB gene fragments were detected directly in these three foods by PCR...
  12. ncbi Surveillance of listeriosis in England and Wales, 1995-1999
    W J Smerdon
    Gastrointestinal Diseases Division, PHLS Communicable Disease Surveillance Centre, 61 Colindale Avenue, London NW9 5EQ
    Commun Dis Public Health 4:188-93. 2001
    ..5 per million conceptions per year. Two episodes of neonatal cross infection occurred. Two non-pregnancy associated cases in 1999 were linked by a common food; all other cases were unlinked. No trend was observed from 1995 to 1999...
  13. ncbi The use of monoclonal antibodies in the characterization and purification of cell surface antigens of Listeria monocytogenes serogroup 4
    J McLauchlin
    Division of Microbiological Reagents and Quality Control, Central Public Health Laboratory, Colindale, London, Britain
    Acta Microbiol Hung 36:459-65. 1989
    ..A group of three antigens of differing electrophoretic mobilities were demonstrated in the affinity purified material...
  14. ncbi Listeria monocytogenes and listeriosis: a review of hazard characterisation for use in microbiological risk assessment of foods
    J McLauchlin
    Health Protection Agency, Food Safety Microbiology Laboratory, Division of Gastrointestinal Infections, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK
    Int J Food Microbiol 92:15-33. 2004
    ..The review is written to provide a clinical and epidemiological background to the mathematically oriented, as well as to outline the mathematical approaches to those interested in food-borne infection...
  15. ncbi Cryptosporidium meleagridis from humans: molecular analysis and description of affected patients
    S Pedraza-Diaz
    Food Safety Microbiology Laboratory, Division of Gastrointestinal Infections, PHLS Central Public Health Laboratory, 61 Colindale Avenue, London, NW9 5HT, UK
    J Infect 42:243-50. 2001
    ..To genetically characterize an unusual genotype of Cryptosporidium from the stools of humans with diarrhoea and to identify risk factors in the affected patients...
  16. ncbi Blinded evaluation of DNA extraction and genotyping of stained Cryptosporidium on glass slides
    C F L Amar
    Food Safety Microbiology Laboratory, PHLS Division of Gastrointestinal Infections, Central Public Health Laboratory, London
    Lett Appl Microbiol 35:486-8. 2002
    ....
  17. pmc Sensitive PCR-restriction fragment length polymorphism assay for detection and genotyping of Giardia duodenalis in human feces
    C F L Amar
    Food Safety Microbiology Laboratory, Division of Gastrointestinal Infections, Public Health Laboratory Service, Central Public Health Laboratory, London NW9 5HT, United Kingdom
    J Clin Microbiol 40:446-52. 2002
    ..The heminested PCR method developed is sensitive, simple, and rapid to perform and is applicable for the analysis of other intestinal pathogens...
  18. ncbi Microbiological safety of sandwiches from hospitals and other health care establishments in the United Kingdom with a focus on Listeria monocytogenes and other Listeria species
    C L Little
    Department of Gastrointestinal, Emerging and Zoonotic Infections, Health Protection Agency Centre for Infections, 61 Colindale Avenue, London NW9 5EQ, UK
    J Food Prot 71:309-18. 2008
    ..monocytogenes in sandwiches at the point of production...
  19. ncbi Amplified fragment length polymorphism (AFLP) analysis of Listeria monocytogenes
    M M Guerra
    CIISA Laboratório de Inspecção Sanitaria, Faculdade de Medicina Veterinária Universidade Técnica de Lisboa, Portugal
    Syst Appl Microbiol 25:456-61. 2002
    ..This preliminary evaluation suggests that this AFLP method will be useful for epidemiological typing of L. monocytogenes...
  20. ncbi Molecular characterisation and antimicrobial resistance of Vibrio vulnificus and Vibrio alginolyticus isolated from mussels (Mytilus galloprovincialis)
    G Ripabelli
    Department of Animal, Plant and Environmental Science, University of Molise, Campobasso, Italy
    Syst Appl Microbiol 26:119-26. 2003
    ..A combination of methods gave optimal discrimination, although a single method could provide sufficient information to characterise V. vulnificus isolates...
  21. doi Microbiological quality of retail cheeses made from raw, thermized or pasteurized milk in the UK
    C L Little
    Health Protection Agency, Department of Gastrointestinal, Emerging and Zoonotic Infections, HPA Centre for Infections, 61 Colindale Avenue, London NW9 5EQ, UK
    Food Microbiol 25:304-12. 2008
    ..Labelling of cheeses with clear information on whether the cheese was prepared from raw milk also requires improvement...
  22. ncbi Prevalence and level of Listeria monocytogenes and other Listeria species in retail pre-packaged mixed vegetable salads in the UK
    C L Little
    Department of Gastrointestinal Infections, Health Protection Agency, HPA Centre for Infections, London, UK
    Food Microbiol 24:711-7. 2007
    ..monocytogenes in food manufacturing and at retail sale is essential in order to minimize the potential for this bacterium to be present in mixed salads at the point of consumption at levels hazardous to health...
  23. ncbi Construction and evaluation of a microbiological positive process internal control for PCR-based examination of food samples for Listeria monocytogenes and Salmonella enterica
    N M Murphy
    Health Protection Agency Food Safety Microbiology Laboratory, Centre for Infections, 61 Colindale Avenue, London NW9 5EQ, United Kingdom
    Int J Food Microbiol 120:110-9. 2007
    ..Control strategies such as those described here are important tools for the interpretation of PCR assays by improving the reliability of detection of pathogens in food...
  24. ncbi Detection by PCR of eight groups of enteric pathogens in 4,627 faecal samples: re-examination of the English case-control Infectious Intestinal Disease Study (1993-1996)
    C F L Amar
    Health Protection Agency Centre for Infections, 61 Colindale Avenue, NW9 5EQ, London, UK
    Eur J Clin Microbiol Infect Dis 26:311-23. 2007
    ..The results demonstrate the greater sensitivity of PCR-based methods compared to current conventional methods...
  25. ncbi Prevalence of Listeria monocytogenes and other Listeria species in butter from United Kingdom production, retail, and catering premises
    H C Lewis
    tGastrointestinal Infections Department, Health Protection Agency Centre for Infections, 61 Colindale Avenue, London, NW9 5EQ, UK
    J Food Prot 69:1518-26. 2006
    ..monocytogenes. The control of L. monocytogenes in food processing and supply systems is critical in order to minimize the potential for this bacterium to be present in foods at the point of consumption at levels hazardous to health...
  26. ncbi Wound botulism in injectors of drugs: upsurge in cases in England during 2004
    D Akbulut
    Health Protection Agency, Centre for Infections, London, United Kingdom
    Euro Surveill 10:172-4. 2005
    ..There was some geographical clustering of the cases during 2004, with most cases occurring in London and in the Yorkshire and Humberside region of northeast England...
  27. ncbi Scombrotoxic fish poisoning
    J McLauchlin
    Health Protection Agency Centre for Infections, 61 Colindale Avenue, London NW9 5EQ, UK
    J Public Health (Oxf) 28:61-2. 2006
  28. pmc Improvement in laboratory diagnosis of wound botulism and tetanus among injecting illicit-drug users by use of real-time PCR assays for neurotoxin gene fragments
    D Akbulut
    Food Safety Microbiology Laboratory, Health Protection Agency Centre for Infections, London, UK
    J Clin Microbiol 43:4342-8. 2005
    ..Recommendations are given for an improved strategy for the laboratory investigation of suspected wound botulism and tetanus among IDUs...
  29. ncbi Development and application of Real-Time PCR assays to detect fragments of the Clostridium botulinum types A, B, and E neurotoxin genes for investigation of human foodborne and infant botulism
    D Akbulut
    Food Safety Microbiology Laboratory, Health Protection Agency Centre for Infections, London, United Kingdom
    Foodborne Pathog Dis 1:247-57. 2004
    ..These assays have already proven useful for pubic health microbiological investigation of suspected cases of human botulism by substantially improving the diagnostic process...
  30. ncbi Characterisation of a Cryptosporidium isolate from water buffalo (Bubalus bubalis) by sequencing of a fragment of the Cryptosporidium oocyst wall protein gene (COWP)
    H Gómez-Couso
    Laboratorio de Parasitologia, Departamento de Microbiologia y Parasitologia, Facultad de Farmacia, Universidad de Santiago de Compostela, Avenida de Vigo s n, 15782 Santiago de Compostela, A Coruna, Spain
    Vet Parasitol 131:139-44. 2005
    ..The COWP gene fragment was cloned and sequencing analyses revealed it to be similar to the C. parvum 'pig' genotype but with four base pairs substitutions...
  31. ncbi Blinded application of microscopy, bacteriological culture, immunoassays and PCR to detect gastrointestinal pathogens from faecal samples of patients with community-acquired diarrhoea
    C F L Amar
    Health Protection Agency, Food Safety Microbiology Laboratory, 61 Colindale Avenue, London, NW9 5HT, UK
    Eur J Clin Microbiol Infect Dis 23:529-34. 2004
    ..coli since these were only investigated by PCR. The results of this small study clearly demonstrate the advantages of PCR-based methods compared to conventional techniques for the detection of gastrointestinal pathogens...
  32. ncbi Prevalence and characterization of Listeria monocytogenes in the feces of healthy Austrians
    K Grif
    , Innsbruck, Austria
    Wien Klin Wochenschr 113:737-42. 2001
    ..Our data indicate that the Austrian fecal carriage rate is at least 0.8%. In view of a listeriosis incidence of 0.16/100,000 per year, the chances of fecal carriage developing into listeriosis appear to be very low...
  33. ncbi Detection of Cryptosporidium and Giardia in molluscan shellfish by multiplexed nested-PCR
    H Gómez-Couso
    Laboratorio de Parasitologia, Departamento de Microbiología y Parasitología Instituto de Acuicultura, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, A Coruna, Spain
    Int J Food Microbiol 91:279-88. 2004
    ..There was no significant association between the level of Escherichia coli detected by conventional microbiological methods and the presence of Cryptosporidium detected by ABC-PCR...
  34. ncbi Detection and identification by real time PCR/RFLP analyses of Cryptosporidium species from human faeces
    C F L Amar
    Food Safety Microbiology Laboratory, Health Protection Agency, London, UK
    Lett Appl Microbiol 38:217-22. 2004
    ..To detect a wide range of Cryptosporidium species from human faeces by analysis of the Cryptosporidium oocyst wall protein gene by PCR...
  35. ncbi A case of infant botulism with a possible link to infant formula milk powder: evidence for the presence of more than one strain of Clostridium botulinum in clinical specimens and food
    M M Brett
    Health Protection Agency Food Safety Microbiology Laboratory, Centre for Infections, 61 Colindale Avenue, London NW9 5HT, UK
    J Med Microbiol 54:769-76. 2005
    ..It is suggested that multiple C. botulinum were present in both food and the intestine during infant botulism...