Karl Swann

Summary

Affiliation: Cardiff University
Country: UK

Publications

  1. pmc Regulation of diacylglycerol production and protein kinase C stimulation during sperm- and PLCzeta-mediated mouse egg activation
    Yuansong Yu
    Department of Obstetrics and Gynaecology, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, U K
    Biol Cell 100:633-43. 2008
  2. pmc Divergent effect of mammalian PLCζ in generating Ca²⁺ oscillations in somatic cells compared with eggs
    Sally V Phillips
    Department of Obstetrics and Gynaecology, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UK
    Biochem J 438:545-53. 2011
  3. pmc Phospholipase C-ζ-induced Ca2+ oscillations cause coincident cytoplasmic movements in human oocytes that failed to fertilize after intracytoplasmic sperm injection
    Karl Swann
    School of Medicine, Cardiff University, Heath Park, Cardiff, United Kingdom
    Fertil Steril 97:742-7. 2012
  4. doi request reprint The dynamics of calcium oscillations that activate mammalian eggs
    Karl Swann
    Department of Obstetrics and Gynaecology, School of Medicine, Cardiff University, Cardiff, UK
    Int J Dev Biol 52:585-94. 2008
  5. ncbi request reprint Role of phospholipase C-zeta domains in Ca2+-dependent phosphatidylinositol 4,5-bisphosphate hydrolysis and cytoplasmic Ca2+ oscillations
    Michail Nomikos
    Cell Signalling Laboratory, Wales Heart Research Institute, School of Medicine, Cardiff University, Cardiff CF14 4XN
    J Biol Chem 280:31011-8. 2005
  6. pmc Phospholipase Cζ rescues failed oocyte activation in a prototype of male factor infertility
    Michail Nomikos
    Cardiff University School of Medicine, Heath Park, Cardiff, United Kingdom
    Fertil Steril 99:76-85. 2013
  7. pmc PLCζ causes Ca(2+) oscillations in mouse eggs by targeting intracellular and not plasma membrane PI(4,5)P(2)
    Yuansong Yu
    Institute of Molecular and Experimental Medicine, Cardiff University School of Medicine, Cardiff CF14 4XN, United Kingdom
    Mol Biol Cell 23:371-80. 2012
  8. pmc Phospholipase Cζ binding to PtdIns(4,5)P2 requires the XY-linker region
    Michail Nomikos
    Cell Signalling Laboratory, Wales Heart Research Institute, Cardiff University School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UK
    J Cell Sci 124:2582-90. 2011
  9. pmc Male infertility-linked point mutation disrupts the Ca2+ oscillation-inducing and PIP(2) hydrolysis activity of sperm PLCζ
    Michail Nomikos
    Cell Signalling Laboratory, Wales Heart Research Institute, Cardiff University School of Medicine, Cardiff CF14 4XN, UK
    Biochem J 434:211-7. 2011
  10. pmc Novel regulation of PLCζ activity via its XY-linker
    Michail Nomikos
    Department of Obstetrics and Gynaecology, Cardiff University School of Medicine, Cardiff CF14 4XN, UK
    Biochem J 438:427-32. 2011

Collaborators

Detail Information

Publications21

  1. pmc Regulation of diacylglycerol production and protein kinase C stimulation during sperm- and PLCzeta-mediated mouse egg activation
    Yuansong Yu
    Department of Obstetrics and Gynaecology, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, U K
    Biol Cell 100:633-43. 2008
    ..Fertilization-mediated increase in PKC activity has been demonstrated; however, its precise role is unclear...
  2. pmc Divergent effect of mammalian PLCζ in generating Ca²⁺ oscillations in somatic cells compared with eggs
    Sally V Phillips
    Department of Obstetrics and Gynaecology, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UK
    Biochem J 438:545-53. 2011
    ..These data suggest that PLCζ-mediated Ca²⁺ oscillations may require specific factors that are only present within the egg cytoplasm or be inhibited by factors present only in somatic cell lines...
  3. pmc Phospholipase C-ζ-induced Ca2+ oscillations cause coincident cytoplasmic movements in human oocytes that failed to fertilize after intracytoplasmic sperm injection
    Karl Swann
    School of Medicine, Cardiff University, Heath Park, Cardiff, United Kingdom
    Fertil Steril 97:742-7. 2012
    ..To evaluate the imaging of cytoplasmic movements in human oocytes as a potential method to monitor the pattern of Ca(2+) oscillations during activation...
  4. doi request reprint The dynamics of calcium oscillations that activate mammalian eggs
    Karl Swann
    Department of Obstetrics and Gynaecology, School of Medicine, Cardiff University, Cardiff, UK
    Int J Dev Biol 52:585-94. 2008
    ..As yet we do not fully understand how the target of calcium in a mammalian egg might decode the patterns of calcium changes that can occur during egg activation...
  5. ncbi request reprint Role of phospholipase C-zeta domains in Ca2+-dependent phosphatidylinositol 4,5-bisphosphate hydrolysis and cytoplasmic Ca2+ oscillations
    Michail Nomikos
    Cell Signalling Laboratory, Wales Heart Research Institute, School of Medicine, Cardiff University, Cardiff CF14 4XN
    J Biol Chem 280:31011-8. 2005
    ..These data suggest that the exquisite Ca2+-dependent features of PLCzeta regulation are essential for it to generate inositol 1,4,5-trisphosphate and Ca2+ oscillations in intact mouse eggs...
  6. pmc Phospholipase Cζ rescues failed oocyte activation in a prototype of male factor infertility
    Michail Nomikos
    Cardiff University School of Medicine, Heath Park, Cardiff, United Kingdom
    Fertil Steril 99:76-85. 2013
    ....
  7. pmc PLCζ causes Ca(2+) oscillations in mouse eggs by targeting intracellular and not plasma membrane PI(4,5)P(2)
    Yuansong Yu
    Institute of Molecular and Experimental Medicine, Cardiff University School of Medicine, Cardiff CF14 4XN, United Kingdom
    Mol Biol Cell 23:371-80. 2012
    ....
  8. pmc Phospholipase Cζ binding to PtdIns(4,5)P2 requires the XY-linker region
    Michail Nomikos
    Cell Signalling Laboratory, Wales Heart Research Institute, Cardiff University School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UK
    J Cell Sci 124:2582-90. 2011
    ....
  9. pmc Male infertility-linked point mutation disrupts the Ca2+ oscillation-inducing and PIP(2) hydrolysis activity of sperm PLCζ
    Michail Nomikos
    Cell Signalling Laboratory, Wales Heart Research Institute, Cardiff University School of Medicine, Cardiff CF14 4XN, UK
    Biochem J 434:211-7. 2011
    ..Wild-type PLCζ initiated a normal pattern of Ca2+ oscillations in eggs in the presence of 10-fold higher mutant PLCζ, suggesting that infertility is not mediated by a dominant-negative mechanism...
  10. pmc Novel regulation of PLCζ activity via its XY-linker
    Michail Nomikos
    Department of Obstetrics and Gynaecology, Cardiff University School of Medicine, Cardiff CF14 4XN, UK
    Biochem J 438:427-32. 2011
    ....
  11. doi request reprint Starting a new life: sperm PLC-zeta mobilizes the Ca2+ signal that induces egg activation and embryo development: an essential phospholipase C with implications for male infertility
    Michail Nomikos
    Cell Signalling Laboratory, WHRI, Institute of Molecular and Experimental Medicine, Cardiff University School of Medicine, Cardiff, UK
    Bioessays 34:126-34. 2012
    ..Recent advances have refined our understanding of how PLCζ induces Ca(2+) oscillations in the egg and also suggest its potential dysfunction as a cause of male infertility...
  12. ncbi request reprint PLC zeta: a sperm-specific trigger of Ca(2+) oscillations in eggs and embryo development
    Christopher M Saunders
    Cell Signalling Laboratory, Wales Heart Research Institute, University of Wales College of Medicine, Cardiff CF14 4XN, UK
    Development 129:3533-44. 2002
    ..Our results are consistent with sperm PLC zeta as the molecular trigger for development of a fertilised egg into an embryo...
  13. pmc Human PLCζ exhibits superior fertilization potency over mouse PLCζ in triggering the Ca(2+) oscillations required for mammalian oocyte activation
    Michail Nomikos
    Cardiff University School of Medicine, Heath Park, Cardiff CF14 4XN, UK
    Mol Hum Reprod 20:489-98. 2014
    ..Our empirical observations are compatible with a basic mathematical model for the Ca(2+) dependence of generating cytoplasmic Ca(2+) oscillations in mammalian oocytes by sperm PLCζ. ..
  14. pmc Chimeras of sperm PLCζ reveal disparate protein domain functions in the generation of intracellular Ca2+ oscillations in mammalian eggs at fertilization
    Maria Theodoridou
    Institute of Molecular and Experimental Medicine, WHRI, Cardiff University School of Medicine, Cardiff CF14 4XN, UK
    Mol Hum Reprod 19:852-64. 2013
    ..These numerical simulations can for the first time predict the empirical variability in onset and frequency of Ca(2+) oscillatory activity associated with specific PLC variants. ..
  15. pmc The dynamics of PKC-induced phosphorylation triggered by Ca2+ oscillations in mouse eggs
    Jose Raul Gonzalez-Garcia
    Institute of Molecular and Experimental Medicine, School of Medicine, Cardiff University, Cardiff, UK
    J Cell Physiol 228:110-9. 2013
    ..The results also suggest that Ca(2+) -induced DAG formation on intracellular membranes may stimulate PKC activity oscillations at fertilization...
  16. pmc Redistribution of mitochondria leads to bursts of ATP production during spontaneous mouse oocyte maturation
    Yuansong Yu
    Department of Obstetrics and Gynaecology, School of Medicine, Cardiff University, Heath Park, Cardiff, UK
    J Cell Physiol 224:672-80. 2010
    ....
  17. doi request reprint Use of luciferase chimaera to monitor PLCzeta expression in mouse eggs
    Karl Swann
    Department of Obstetrics and Gynaecology, School of Medicine, Cardiff University, Cardiff, UK
    Methods Mol Biol 518:17-29. 2009
    ....
  18. ncbi request reprint Ca2+ oscillations stimulate an ATP increase during fertilization of mouse eggs
    Karen Campbell
    Department of Obstetrics and Gynaecology, School of Medicine, Cardiff University, Cardiff CF14 4XN, UK
    Dev Biol 298:225-33. 2006
    ..These data suggest that mammalian fertilization is associated with a sudden but transient increase in cytosolic ATP and that Ca(2+) oscillations are both necessary and sufficient to cause this increase in ATP levels...
  19. doi request reprint PLCζ and the initiation of Ca(2+) oscillations in fertilizing mammalian eggs
    Karl Swann
    Institute of Molecular and Experimental Medicine, Cardiff University School of Medicine, Heath Park, Cardiff CF14 4XN, UK
    Cell Calcium 53:55-62. 2013
    ..This leads us to suggest that sperm PLCζ may be targeted to these cytoplasmic vesicles by directly interacting with a specific but as yet unidentified egg PLCζ-binding protein...
  20. pmc The dynamics of MAPK inactivation at fertilization in mouse eggs
    Jose Raul Gonzalez-Garcia
    Institute of Molecular and Experimental Medicine, School of Medicine, Cardiff University, Cardiff CF14 4XN, UK
    J Cell Sci 127:2749-60. 2014
    ..These data suggest that the completion of meiosis in mouse zygotes is driven by an increased phosphatase activity and not by a decline in Mos levels or MEK activity. ..
  21. doi request reprint Measuring Ca2+ oscillations in mammalian eggs
    Karl Swann
    School of Medicine, Cardiff University, Heath Park, Cardiff, UK
    Methods Mol Biol 957:231-48. 2013
    ..Either one of these dyes offers advantages over the more commonly used fura2. I describe the way that the fluorescence from dye-loaded eggs is measured with a conventional epifluorescence microscope and a CCD camera...