Research Topics
| Karl SwannSummaryAffiliation: Cardiff University Country: UK Publications
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Detail Information
Publications
Phospholipase C-ζ-induced Ca2+ oscillations cause coincident cytoplasmic movements in human oocytes that failed to fertilize after intracytoplasmic sperm injectionKarl Swann
School of Medicine, Cardiff University, Heath Park, Cardiff, United Kingdom
Fertil Steril 97:742-7. 2012..To evaluate the imaging of cytoplasmic movements in human oocytes as a potential method to monitor the pattern of Ca(2+) oscillations during activation...
The dynamics of calcium oscillations that activate mammalian eggsKarl Swann
Department of Obstetrics and Gynaecology, School of Medicine, Cardiff University, Cardiff, UK
Int J Dev Biol 52:585-94. 2008..As yet we do not fully understand how the target of calcium in a mammalian egg might decode the patterns of calcium changes that can occur during egg activation...
Phospholipase Cζ rescues failed oocyte activation in a prototype of male factor infertilityMichail Nomikos
Cardiff University School of Medicine, Heath Park, Cardiff, United Kingdom
Fertil Steril 99:76-85. 2013....
Divergent effect of mammalian PLCζ in generating Ca²⁺ oscillations in somatic cells compared with eggsSally V Phillips
Department of Obstetrics and Gynaecology, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UK
Biochem J 438:545-53. 2011..These data suggest that PLCζ-mediated Ca²⁺ oscillations may require specific factors that are only present within the egg cytoplasm or be inhibited by factors present only in somatic cell lines...
Starting a new life: sperm PLC-zeta mobilizes the Ca2+ signal that induces egg activation and embryo development: an essential phospholipase C with implications for male infertilityMichail Nomikos
Cell Signalling Laboratory, WHRI, Institute of Molecular and Experimental Medicine, Cardiff University School of Medicine, Cardiff, UK
Bioessays 34:126-34. 2012..Recent advances have refined our understanding of how PLCζ induces Ca(2+) oscillations in the egg and also suggest its potential dysfunction as a cause of male infertility...
Novel regulation of PLCζ activity via its XY-linkerMichail Nomikos
Department of Obstetrics and Gynaecology, Cardiff University School of Medicine, Cardiff CF14 4XN, UK
Biochem J 438:427-32. 2011....
Regulation of diacylglycerol production and protein kinase C stimulation during sperm- and PLCzeta-mediated mouse egg activationYuansong Yu
Department of Obstetrics and Gynaecology, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, U K
Biol Cell 100:633-43. 2008..Fertilization-mediated increase in PKC activity has been demonstrated; however, its precise role is unclear...
The dynamics of PKC-induced phosphorylation triggered by Ca2+ oscillations in mouse eggsJose Raul Gonzalez-Garcia
Institute of Molecular and Experimental Medicine, School of Medicine, Cardiff University, Cardiff, UK
J Cell Physiol 228:110-9. 2013..The results also suggest that Ca(2+) -induced DAG formation on intracellular membranes may stimulate PKC activity oscillations at fertilization...
Role of phospholipase C-zeta domains in Ca2+-dependent phosphatidylinositol 4,5-bisphosphate hydrolysis and cytoplasmic Ca2+ oscillationsMichail Nomikos
Cell Signalling Laboratory, Wales Heart Research Institute, School of Medicine, Cardiff University, Cardiff CF14 4XN
J Biol Chem 280:31011-8. 2005..These data suggest that the exquisite Ca2+-dependent features of PLCzeta regulation are essential for it to generate inositol 1,4,5-trisphosphate and Ca2+ oscillations in intact mouse eggs...
Phospholipase Cζ binding to PtdIns(4,5)P2 requires the XY-linker regionMichail Nomikos
Cell Signalling Laboratory, Wales Heart Research Institute, Cardiff University School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UK
J Cell Sci 124:2582-90. 2011....
Redistribution of mitochondria leads to bursts of ATP production during spontaneous mouse oocyte maturationYuansong Yu
Department of Obstetrics and Gynaecology, School of Medicine, Cardiff University, Heath Park, Cardiff, UK
J Cell Physiol 224:672-80. 2010....
Use of luciferase chimaera to monitor PLCzeta expression in mouse eggsKarl Swann
Department of Obstetrics and Gynaecology, School of Medicine, Cardiff University, Cardiff, UK
Methods Mol Biol 518:17-29. 2009....
Measuring Ca2+ oscillations in mammalian eggsKarl Swann
School of Medicine, Cardiff University, Heath Park, Cardiff, UK
Methods Mol Biol 957:231-48. 2013..Either one of these dyes offers advantages over the more commonly used fura2. I describe the way that the fluorescence from dye-loaded eggs is measured with a conventional epifluorescence microscope and a CCD camera...
Ca2+ oscillations stimulate an ATP increase during fertilization of mouse eggsKaren Campbell
Department of Obstetrics and Gynaecology, School of Medicine, Cardiff University, Cardiff CF14 4XN, UK
Dev Biol 298:225-33. 2006..These data suggest that mammalian fertilization is associated with a sudden but transient increase in cytosolic ATP and that Ca(2+) oscillations are both necessary and sufficient to cause this increase in ATP levels...
PLCζ and the initiation of Ca(2+) oscillations in fertilizing mammalian eggsKarl Swann
Institute of Molecular and Experimental Medicine, Cardiff University School of Medicine, Heath Park, Cardiff CF14 4XN, UK
Cell Calcium 53:55-62. 2013..This leads us to suggest that sperm PLCζ may be targeted to these cytoplasmic vesicles by directly interacting with a specific but as yet unidentified egg PLCζ-binding protein...
