Genomes and Genes
Stephen R Sturzenbaum
Affiliation: Cardiff University
- Cadmium detoxification in earthworms: from genes to cellsStephen R Sturzenbaum
Cardiff University, School of Biosciences, Main Building, Cardiff, CF10 3TL UK
Environ Sci Technol 38:6283-9. 2004..In summary, this paper provides a detailed dissection of the genetic, molecular, and cellular basis of a sophisticated pathway that facilitates the uptake, accumulation, transport, and excretion of cadmium...
- Identification of heavy metal induced changes in the expression patterns of the translationally controlled tumour protein (TCTP) in the earthworm Lumbricus rubellus1S R Stürzenbaum
School of Molecular and Medical Biosciences, University of Wales, P O Box 911, Cardiff CF1 3US, Wales, UK
Biochim Biophys Acta 1398:294-304. 1998....
- Molecular cloning and characterization of Cpn60 in the free-living nematode Plectus acuminatusS R Stürzenbaum
Cardiff School of Biosciences, Cardiff University, PO Box 915, Cardiff CF10 3TL, Wales, UK
Cell Stress Chaperones 10:79-85. 2005..In conclusion, this first account of molecular genetic similarities and differences of Cpn60 in a neglected nematode taxon provides a valuable insight into its potential uses in gene-based ecotoxicological risk assessment exercises...
- Metal ion trafficking in earthworms. Identification of a cadmium-specific metallothioneinS R Stürzenbaum
School of Biosciences, P O Box 911, Cardiff University, Cardiff CF1 3US, Wales, United Kingdom
J Biol Chem 276:34013-8. 2001..The structure-function relationship of wMT-2 and its role in cadmium detoxification through sequestration and compartmentalization is discussed...
- The identification, cloning and characterization of earthworm metallothioneinS R Stürzenbaum
School of Molecular and Medical Biosciences, University of Wales, Cardiff, UK
FEBS Lett 431:437-42. 1998..Possible implications for intracellular metal trafficking are discussed...
- Characterisation and quantification of earthworm cyclophilins: identification of invariant and heavy metal responsive isoformsS R Stürzenbaum
School of Biosciences, University of Wales, Cardiff, UK
Biochim Biophys Acta 1489:467-73. 1999..This intriguing observation has profound implications regarding cyclophilin's use as an invariant control and highlights the fact that it is essential to treat cyclophilin isoforms as separate entities, rather than one functional unit...
- Developing a new method for soil pollution monitoring using molecular genetic biomarkersM Galay-Burgos
Cardiff School of Biosciences, Cardiff University, PO Box 915, Cardiff, CF10 3TL, UK
Biomarkers 8:229-39. 2003..This study demonstrates that it is now technically feasible to use gene expression as an index of pollution exposure in environmentally relevant organisms...
- Accumulated metal speciation in earthworm populations with multigenerational exposure to metalliferous soils: cell fractionation and high-energy synchrotron analysesJane Andre
Cardiff School of Biosciences, Cardiff University, Park Place, Cardiff CF10 3US, UK
Environ Sci Technol 43:6822-9. 2009..These observations are highly significant in the challenging exercise of interpreting holistic biomarker data delivered by "omic" technologies...
- Knock down of Caenorhabditis elegans cutc-1 exacerbates the sensitivity toward high levels of copperSara Calafato
School of Biosciences, University of Cardiff, Main Building, Park Place, Cardiff CF10 3TL, UK
Toxicol Sci 106:384-91. 2008..This indicates that cutc-1 plays a crucial role in the protection from excess copper...
- Application of physiologically based modelling and transcriptomics to probe the systems toxicology of aldicarb for Caenorhabditis elegans (Maupas 1900)Jodie F Wren
School of Biosciences, Cardiff University, Park Place, Cardiff, UK
Ecotoxicology 20:397-408. 2011..These effects, coupled to the effect on biotransformation enzymes also seen, represent the materialisation of the maintenance costs indicated by DEBtox modelling...
- Cu and Cd effects on the earthworm Lumbricus rubellus in the laboratory: multivariate statistical analysis of relationships between exposure, biomarkers, and ecologically relevant parametersMalyka Galay Burgos
School of Biosciences, Cardiff University, Cardiff CF10 3US, UK
Environ Sci Technol 39:1757-63. 2005..It is proposed that the use of such a suite of biomarkers could serve as indicators of the "health" of the soil environment and provide early warning signals of potential danger to the biota or as a means of monitoring soil remediation...
- C. elegans metallothioneins: new insights into the phenotypic effects of cadmium toxicosisS C Swain
Cardiff University, School of Biosciences, Wales, UK
J Mol Biol 341:951-59. 2004..elegans upon exposure to cadmium, the results providing further evidence that the transcriptional control of metallothioneins is fundamentally divergent in lower invertebrates and not mediated via MTF-1 as in more complex organisms...
- Differential metallothionein expression in earthworm (Lumbricus rubellus) tissuesA J Morgan
Cardiff School of Biosciences, Cardiff University, PO Box 915, Cardiff Wales, CF10 3TL, UK
Ecotoxicol Environ Saf 57:11-9. 2004....
- Control genes in quantitative molecular biological techniques: the variability of invarianceS R Stürzenbaum
Cardiff University, School of Biosciences BIOSI 2, P O Box 911, Wales, Cardiff, UK
Comp Biochem Physiol B Biochem Mol Biol 130:281-9. 2001..Finally, different approaches undertaken to assess the invariance of control genes are critically analysed to generate a procedure that will help to discern the best control for novel experiments...
- Molecular genetic differentiation in earthworms inhabiting a heterogeneous Pb-polluted landscapeJ Andre
Cardiff School of Biosciences, Cardiff University, BIOSI 1, Museum Avenue, Cardiff CF10 3TL, UK
Environ Pollut 158:883-90. 2010..We recommend the mandatory genotyping of all individuals prior to field-based ecotoxicological assays, particularly those using discriminating genomic technologies...
- Earthworm pre-procarboxypeptidase: a copper responsive enzymeS R Stürzenbaum
School of Biosciences, University of Wales, Cardiff, UK
Biometals 14:85-94. 2001..Finally, the significance of these observations in terms of risk assessment and biomonitoring of contaminated soils is discussed...
- Single and double metallothionein knockout in the nematode C. elegans reveals cadmium dependent and independent toxic effects on life history traitsSam Hughes
School of Biosciences, Cardiff University, Main Building, Park Place, Cardiff CF10 3TL, UK
Environ Pollut 145:395-400. 2007..In summary, metallothionein is undoubtedly an important player in cadmium detoxification, but evidently also an important factor in cadmium independent pathways...
- Microevolution and ecotoxicology of metals in invertebratesA John Morgan
Cardiff School of Biosciences, Cardiff University, Cardiff, UK
Environ Sci Technol 41:1085-96. 2007....