Research Topics
| Paul J SmithSummaryAffiliation: Cardiff University Country: UK Publications
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Detail Information
Publications
Kinetic analysis of intracellular Hoechst 33342--DNA interactions by flow cytometry: misinterpretation of side population status?Paul J Smith
Institute of Cancer and Genetics, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, United Kingdom
Cytometry A 83:161-9. 2013..The SP phenotype should be regarded as a kinetic parameter and not a fixed characteristic--critical for functional assay design and the interpretation of heterogeneity...
Cytomics and drug developmentPaul J Smith
Department of Pathology, Wales College of Medicine, Cardiff University, United Kingdom
Cytometry A 71:349-51. 2007- Cytomics and cellular informatics--coping with asymmetry and heterogeneity in biological systemsPaul J Smith
Department of Pathology, Tenovus Building, School of Medicine, Cardiff University, Heath Park, Cardiff, UK
Drug Discov Today 14:271-7. 2009..Advances in all these key areas will help to address the need to identify and evaluate the origins of asymmetry and heterogeneity in cellular systems and thus reveal new opportunities for therapeutic targeting... 
Impact of overexpression of metallothionein-1 on cell cycle progression and zinc toxicityPaul J Smith
Dept of Pathology, Tenovus Bldg, School of Medicine, Cardiff Univ, Heath Park, Cardiff CF14 4XN, UK
Am J Physiol Cell Physiol 295:C1399-408. 2008..The results have implications for the use of cellular stress responses to exogenously supplied zinc and zinc-based systemic therapies...- Mitotic bypass via an occult cell cycle phase following DNA topoisomerase II inhibition in p53 functional human tumor cellsPaul J Smith
Department of Pathology, University of Wales College of Medicine, Heath Park, Cardiff, UK
Cell Cycle 6:2071-81. 2007.... - DNA damage-induced [Zn(2+)](i) transients: correlation with cell cycle arrest and apoptosis in lymphoma cellsPaul J Smith
Department of Pathology, University of Wales, College of Medicine, Health Park, Cardiff, United Kingdom
Am J Physiol Cell Physiol 283:C609-22. 2002..Early stress responses in p53(wt) cells take place in an environment of enhanced Zn(2+) availability... - Characteristics of a novel deep red/infrared fluorescent cell-permeant DNA probe, DRAQ5, in intact human cells analyzed by flow cytometry, confocal and multiphoton microscopyP J Smith
Department of Pathology, University of Wales College of Medicine, Cardiff, United Kingdom
Cytometry 40:280-91. 2000..We describe here the spectral characteristics and applications of this synthetic compound, particularly in relation to cytometric analysis of the cell cycle... - A novel cell permeant and far red-fluorescing DNA probe, DRAQ5, for blood cell discrimination by flow cytometryP J Smith
Department of Pathology, University of Wales, College of Medicine, Heath Park, Cardiff, UK
J Immunol Methods 229:131-9. 1999..Importantly, DRAQ5 can be used in combination with FITC and RPE-labelled antibodies, without the need for fluorescence compensation... - Cell cycle checkpoint evasion and protracted cell cycle arrest in X-irradiated small-cell lung carcinoma cellsP J Smith
Department of Pathology, University of Wales College of Medicine, Health Park, Cardiff, UK
Int J Radiat Biol 75:1137-47. 1999.... - ABCG2-associated resistance to Hoechst 33342 and topotecan in a murine cell model with constitutive expression of side population characteristicsPaul J Smith
Department of Pathology, School of Medicine, Cardiff University, Cardiff CF14 4XN, United Kingdom
Cytometry A 75:924-33. 2009..The study has implications for the patterns of drug resistance arising during tumor repopulation and the basal resistance to minor groove-binding drugs of tumor side-populations... - Microtubule stress modifies intra-nuclear location of Msh2 in mouse embryonic fibroblastsNuria Marquez
Department of Pathology, University of Wales College of Medicine, Heath Park, Cardiff, UK
Cell Cycle 3:662-71. 2004.... - Spectral analysis of the DNA targeting bisalkylaminoanthraquinone DRAQ5 in intact living cellsKerenza L Njoh
Department of Pathology, School of Medicine, Cardiff University, Heath Park, Cardiff, UK
Cytometry A 69:805-14. 2006..Our aim was to determine the constraints for its use in flow cytometry and bioimaging... - Single cell nanoparticle tracking to model cell cycle dynamics and compartmental inheritanceRachel J Errington
School of Medicine, Cardiff University, Cardiff, UK
Cell Cycle 9:121-30. 2010.... - Interoperability of time series cytometric data: a cross platform approach for modeling tumor heterogeneityImtiaz A Khan
Department of Pathology, Tenovus Building, School of Medicine, Cardiff University, Heath Park, Cardiff, United Kingdom
Cytometry A 79:214-26. 2011..This establishes the foundation for developing predictive models and in silico tracking and prediction of tumor progression.. - Flow-based cytometric analysis of cell cycle via simulated cell populationsM Rowan Brown
School of Medicine, Cardiff University, Cardiff, United Kingdom
PLoS Comput Biol 6:e1000741. 2010.... - Long-term time series analysis of quantum dot encoded cells by deconvolution of the autofluorescence signalM Rowan Brown
Department of Infection, Immunity and Biochemistry, School of Medicine, Cardiff University, Heath Park, Cardiff CF144XN, United Kingdom
Cytometry A 77:925-32. 2010..This systems biology approach provides insight into both cell heterogeneity and division dynamics within the population and furthermore informs on the lineage history of its members... - DRAQ5 labeling of nuclear DNA in live and fixed cellsPaul J Smith
University of Wales College of Medicine, Heath Park, Cardiff, United Kingdom
Curr Protoc Cytom . 2004..While the focus is on flow cytometric assays, typical imaging applications are also indicated because the staining protocols share the same essential features... - Single cell tracking reveals that Msh2 is a key component of an early-acting DNA damage-activated G2 checkpointNuria Marquez
Department of Pathology, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XN, UK
Oncogene 22:7642-8. 2003..The findings suggest a new route to MMR-driven genetic instability that does not rely primarily on the integrity of the late-acting checkpoint... - Time-lapse microscopy approaches to track cell cycle progression at the single-cell levelRachel J Errington
School of Medicine, Heath Park, Cardiff, United Kingdom
Curr Protoc Cytom . 2005..The objective of this unit is to present a generic methodology for capturing time-lapse sequences and subsequently mining the data for comprehensive event analysis... - Cytotoxic action of phorbol esters on human pancreatic cancer cellsJane A Bond
Department of Pathology, School of Medicine, Cardiff University, Cardiff CF14 4XN, United Kingdom
Int J Cancer 121:1445-54. 2007.... - ProgeniDB: a novel cell lineage database for generation associated phenotypic behavior in cell-based assaysImtiaz A Khan
Biostatistics and Bioinformatics Unit, School of Medicine, Cardiff University, Cardiff, UK
Cell Cycle 6:868-74. 2007..A case study is presented where we interrogate drug-induced perturbations on ensuing cell lineages. The database is available at http://biodiversity.cs.cf.ac.uk/cymart/progenidb.html... - Time-lapse microscopy approaches to track cell cycle and lineage progression at the single-cell levelRachel J Errington
Institute of Cancer and Genetics, School of Medicine, Cardiff University, Cardiff, United Kingdom
Curr Protoc Cytom . 2013..The objective of this unit is to present the basic methodology for capturing time-lapse sequences and touch upon subsequent mining of the data for deriving event curves and possible cell lineage maps... - A quantifiable proliferative burst of tissue macrophages restores homeostatic macrophage populations after acute inflammationLuke C Davies
Department of Infection, Immunity and Biochemistry, Cardiff University School of Medicine, Heath Park, Cardiff, UK
Eur J Immunol 41:2155-64. 2011.... - Technique for measurement of fluorescence lifetime by use of stroboscopic excitation and continuous-wave detectionD R Matthews
School of Physics and Astronomy, Cardiff University, 5 The Parade, Cardiff CF24 3YB, UK
Appl Opt 45:2115-23. 2006..An experimental demonstration of the technique that uses a cuvette-based analysis of a carbocyanine dye and for which the excitation source is a 650 nm wavelength, self-pulsing AlGaInP laser diode is compared with the model... - Adenosine-regulated cell proliferation in pituitary folliculostellate and endocrine cells: differential roles for the A(1) and A(2B) adenosine receptorsD A Rees
Department of Medicine, University of Wales College of Medicine, Cardiff, United Kingdom CF14 4XN
Endocrinology 143:2427-36. 2002..These data highlight the differential expression of A(1) and A(2B) adenosine receptors in pituitary cells and provide evidence for opposing effects of adenosine on pituitary folliculostellate and endocrine cell growth... - Chemical synthesis of cell-permeable apoptotic peptides from in vivo produced proteinsThomas Fricke
School of Chemistry, Cardiff University, Park Place, Cardiff, United Kingdom
Bioconjug Chem 22:1763-7. 2011..These results highlight the significant potential for use of such tools in live cells... - Telomere-based proliferative lifespan barriers in Werner-syndrome fibroblasts involve both p53-dependent and p53-independent mechanismsTerence Davis
Department of Pathology, University of Wales College of Medicine, Heath Park, Cardiff, CF14 4XN, UK
J Cell Sci 116:1349-57. 2003.... - Stroboscopic fluorescence lifetime imagingMark D Holton
School of Medicine, Swansea University, Singleton Park, Swansea, UK
Opt Express 17:5205-16. 2009..This stroboscopic technique offers lifetime based imaging at video rates with standard CCD cameras and has application in probing millisecond cell dynamics and in high throughput imaging assays... - Biological activity of activating thyrotrophin receptor mutants: modulation by iodideF Al-Khafaji
Centre for Endocrine and Diabetes Sciences, School of Medicine, Cardiff University, Cardiff, UK
J Mol Endocrinol 34:209-20. 2005..In conclusion, our studies indicate that the biological effects of activating TSHR mutations vary with the ambient iodide supply and could be masked in regions of high iodine intake... - Tracking the cell cycle origins for escape from topotecan action by breast cancer cellsG P Feeney
Department of Pathalogy, University of Wales College of Medicine, Cardiff, UK
Br J Cancer 88:1310-7. 2003..31 microM). Overall, topotecan differentially targeted G1-, S- and G2-phase cells, but many G2 cells were resistant to topotecan, presenting a clear route for cell cycle-mediated drug resistance... - A new point mutation in the luteinising hormone receptor gene in familial and sporadic male limited precocious puberty: genotype does not always correlate with phenotypeB A Evans
Department of Child Health, University of Wales College of Medicine, UK
J Med Genet 33:143-7. 1996..In family 2, the index case was the only one to have the mutation. A previously unreported neutral dimorphism (C --> T at nucleotide 1065) is also described... - Advanced microscopy solutions for monitoring the kinetics and dynamics of drug-DNA targeting in living cellsR J Errington
Department of Medical Biochemistry and Immunology, University of Wales College of Medicine, Cardiff, CF14 4XN, UK
Adv Drug Deliv Rev 57:153-67. 2005..Finally, we discuss the future perspectives on introducing these modular kinetic assays in the high-content screening arena and the interlinking of the consequences of drug-target interactions with cellular stress responses... - Solid-phase synthesis of the cyclic peptide portion of chlorofusin, an inhibitor of p53-MDM2 interactionsJohn P Malkinson
Department of Pharmaceutical and Biological Chemistry, School of Pharmacy, University of London, 29-39 Brunswick Square, London WC1N 1AX, UK
Org Lett 5:5051-4. 2003..Initial biological assays, using an immunofluorescence method, suggest that the compounds are not cytotoxic but do not inhibit the p53/mdm2 interaction. [structure: see text].. - Development of nonsymmetrical 1,4-disubstituted anthraquinones that are potently active against cisplatin-resistant ovarian cancer cellsKlaus Pors
Department of Pharmaceutical and Biological Chemistry, The School of Pharmacy, University of London, 29-39 Brunswick Square, London
J Med Chem 48:6690-5. 2005..8 and its chloropropyl analogue 13 retained significant activity against human A2780/cp70 xenografted tumors in mice... - A mathematical model for the in vitro kinetics of the anti-cancer agent topotecanNeil D Evans
School of Engineering, University of Warwick, Coventry CV4 7AL, UK
Math Biosci 189:185-217. 2004..Parameter estimation is performed using experimental data which offers concentrations of active and inactive forms of topotecan from high performance liquid chromatography methods... - Probing the lowest energy chlorophyll a states of photosystem II via selective spectroscopy: new insights on P680Joseph L Hughes
Research School of Chemistry, Australian National University, Building 35, Science Road, Canberra, ACT 0200, Australia
Photosynth Res 84:93-8. 2005..3 dependence found in many chlorophyll-protein systems. Our data indicates T 2 temperature dependence, typically found in crystalline systems where the chromophore is coupled to localized phonon modes... - Exploration of the intercellular heterogeneity of topotecan uptake into human breast cancer cells through compartmental modellingS Y Amy Cheung
School of Pharmacy and Pharmaceutical Sciences, University of Manchester, M13 9PT, UK
Math Biosci 213:119-34. 2008..The identifiability analysis demonstrated how valuable symbolic computation is in this context, as the analysis is far too lengthy and difficult to be performed by hand... - Critical structural motif for the catalytic inhibition of human topoisomerase II by UK-1 and analogsBen B Wang
Department of Chemistry and Biochemistry, University of Maryland, Baltimore County, Baltimore, MD 21250, USA
Bioorg Med Chem Lett 14:3221-6. 2004..Results show that UK-1 and two of these analogs are catalytic inhibitors of topo II and identifies a critical structure motif necessary for enzyme inhibition...