C L Harris

Summary

Affiliation: Cardiff University
Country: UK

Publications

  1. pmc Sensitive and specific assays for C3 nephritic factors clarify mechanisms underlying complement dysregulation
    Danielle Paixao-Cavalcante
    Institute of Infection and Immunity, School of Medicine, Cardiff University, Cardiff, UK
    Kidney Int 82:1084-92. 2012
  2. pmc The complotype: dictating risk for inflammation and infection
    Claire L Harris
    Institute of Infection and Immunity, School of Medicine, Cardiff University, Heath Park, Cardiff, CF14 4XN, UK
    Trends Immunol 33:513-21. 2012
  3. pmc Prevention of experimental autoimmune myasthenia gravis by rat Crry-Ig: A model agent for long-term complement inhibition in vivo
    Natalie J Hepburn
    Department of Medical Biochemistry and Immunology, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, United Kingdom
    Mol Immunol 45:395-405. 2008
  4. ncbi request reprint Efficient generation of monoclonal antibodies for specific protein domains using recombinant immunoglobulin fusion proteins: pitfalls and solutions
    Claire L Harris
    Department of Medical Biochemistry, University of Wales College of Medicine, Tenovus Building, Heath Park, Cardiff, CF14 4XX, UK
    J Immunol Methods 268:245-58. 2002
  5. ncbi request reprint Generation of anti-complement "prodrugs": cleavable reagents for specific delivery of complement regulators to disease sites
    Claire L Harris
    Department of Medical Biochemistry and Immunology, University of Wales College of Medicine, Heath Park, Cardiff, CF14 4XN, United Kingdom
    J Biol Chem 278:36068-76. 2003
  6. pmc Characterization of the mouse analogues of CD59 using novel monoclonal antibodies: tissue distribution and functional comparison
    Claire L Harris
    Complement Biology Group, Department of Medical Biochemistry and Immunology, University of Wales College of Medicine, Heath Park, Cardiff, UK
    Immunology 109:117-26. 2003
  7. ncbi request reprint Molecular dissection of interactions between components of the alternative pathway of complement and decay accelerating factor (CD55)
    Claire L Harris
    Complement Biology Group, Department of Medical Biochemistry and Immunology, School of Medicine, Cardiff University, Heath Park, Cardiff, CF14 4XN, United Kingdom
    J Biol Chem 280:2569-78. 2005
  8. ncbi request reprint Tailoring anti-complement therapeutics
    C L Harris
    Complement Biology Group, Department of Medical Biochemistry, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XN, UK
    Biochem Soc Trans 30:1019-26. 2002
  9. pmc Coupling complement regulators to immunoglobulin domains generates effective anti-complement reagents with extended half-life in vivo
    C L Harris
    Department of Medical Biochemistry, University of Wales College of Medicine, Heath Park, Cardiff, UK
    Clin Exp Immunol 129:198-207. 2002
  10. pmc The membrane attack pathway of complement drives pathology in passively induced experimental autoimmune myasthenia gravis in mice
    B P Morgan
    Department of Pathology, School of Medicine, Cardiff University, Cardiff, UK
    Clin Exp Immunol 146:294-302. 2006

Collaborators

Detail Information

Publications48

  1. pmc Sensitive and specific assays for C3 nephritic factors clarify mechanisms underlying complement dysregulation
    Danielle Paixao-Cavalcante
    Institute of Infection and Immunity, School of Medicine, Cardiff University, Cardiff, UK
    Kidney Int 82:1084-92. 2012
    ..Thus, our study provides a rational approach to detect and characterize nephritic factors in patients...
  2. pmc The complotype: dictating risk for inflammation and infection
    Claire L Harris
    Institute of Infection and Immunity, School of Medicine, Cardiff University, Heath Park, Cardiff, CF14 4XN, UK
    Trends Immunol 33:513-21. 2012
    ..Assessing the complotype of an individual will aid prediction of disease risk and inform intervention to reduce or eliminate risk...
  3. pmc Prevention of experimental autoimmune myasthenia gravis by rat Crry-Ig: A model agent for long-term complement inhibition in vivo
    Natalie J Hepburn
    Department of Medical Biochemistry and Immunology, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, United Kingdom
    Mol Immunol 45:395-405. 2008
    ..The long half-life and low immunogenicity of this agent will be useful for therapy in chronic models of inflammatory disease in the rat...
  4. ncbi request reprint Efficient generation of monoclonal antibodies for specific protein domains using recombinant immunoglobulin fusion proteins: pitfalls and solutions
    Claire L Harris
    Department of Medical Biochemistry, University of Wales College of Medicine, Tenovus Building, Heath Park, Cardiff, CF14 4XX, UK
    J Immunol Methods 268:245-58. 2002
    ..We describe potential pitfalls that may be encountered while using Ig fusion proteins as immunogen and demonstrate ways in which to tailor their design for optimal mAb production...
  5. ncbi request reprint Generation of anti-complement "prodrugs": cleavable reagents for specific delivery of complement regulators to disease sites
    Claire L Harris
    Department of Medical Biochemistry and Immunology, University of Wales College of Medicine, Heath Park, Cardiff, CF14 4XN, United Kingdom
    J Biol Chem 278:36068-76. 2003
    ..Such agents, having negligible systemic effects but active at sites of inflammation, represent a paradigm for the next generation of anti-C therapeutics...
  6. pmc Characterization of the mouse analogues of CD59 using novel monoclonal antibodies: tissue distribution and functional comparison
    Claire L Harris
    Complement Biology Group, Department of Medical Biochemistry and Immunology, University of Wales College of Medicine, Heath Park, Cardiff, UK
    Immunology 109:117-26. 2003
    ..These findings demonstrate that the broadly distributed mCD59a is the key regulator of the terminal complement pathway in mice whereas CD59b, expressed only in testis and on sperm, probably plays other roles in vivo...
  7. ncbi request reprint Molecular dissection of interactions between components of the alternative pathway of complement and decay accelerating factor (CD55)
    Claire L Harris
    Complement Biology Group, Department of Medical Biochemistry and Immunology, School of Medicine, Cardiff University, Heath Park, Cardiff, CF14 4XN, United Kingdom
    J Biol Chem 280:2569-78. 2005
    ..Such analysis of component and regulator interactions will inform our understanding of inhibitory mechanisms and the ways in which regulatory proteins cooperate to control the complement cascade...
  8. ncbi request reprint Tailoring anti-complement therapeutics
    C L Harris
    Complement Biology Group, Department of Medical Biochemistry, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XN, UK
    Biochem Soc Trans 30:1019-26. 2002
    ..Finally, we will illustrate a novel approach to the delivery of anticomplement agents, making prodrugs that are activated only at disease sites thus minimizing the deleterious effects of systemic complement inhibition...
  9. pmc Coupling complement regulators to immunoglobulin domains generates effective anti-complement reagents with extended half-life in vivo
    C L Harris
    Department of Medical Biochemistry, University of Wales College of Medicine, Heath Park, Cardiff, UK
    Clin Exp Immunol 129:198-207. 2002
    ..The data demonstrate the potential of CRP-Ig as reagents for sustained therapy of inflammatory disorders, including arthritis, but emphasize the need for careful design of fusion proteins to retain function...
  10. pmc The membrane attack pathway of complement drives pathology in passively induced experimental autoimmune myasthenia gravis in mice
    B P Morgan
    Department of Pathology, School of Medicine, Cardiff University, Cardiff, UK
    Clin Exp Immunol 146:294-302. 2006
    ..These findings provoke us to suggest that current anti-C therapeutics targeting MAC assembly will be beneficial in MG patients resistant to conventional therapies...
  11. ncbi request reprint Efficient generation of monoclonal antibodies against surface-expressed proteins by hyperexpression in rodent cells
    O B Spiller
    Department of Medical Biochemistry, University of Wales College of Medicine, Heath Park, Cardiff, UK
    J Immunol Methods 224:51-60. 1999
    ..The strategy also permits rapid and efficient screening using flow cytometry on expressing cells...
  12. pmc Human and rodent decay-accelerating factors (CD55) are not species restricted in their complement-inhibiting activities
    C L Harris
    Complement Biology Group, Department of Medical Biochemistry, University of Wales College of Medicine, Heath Park, Cardiff, UK
    Immunology 100:462-70. 2000
    ..This has implications in broader fields of research, such as xenotransplantation, where cross-species regulation of complement is of paramount importance...
  13. ncbi request reprint Pigs express multiple forms of decay-accelerating factor (CD55), all of which contain only three short consensus repeats
    J M Perez de la Lastra
    Complement Biology Group, Department of Medical Biochemistry, University of Wales College of Medicine, Heath Park, Cardiff, United Kingdom
    J Immunol 165:2563-73. 2000
    ..The transmembrane isoform of pig DAF was expressed on Chinese hamster ovary cells and was shown to affect regulatory activity for the classical pathway of human complement, but was only marginally active against pig serum...
  14. ncbi request reprint Antigen-presenting cell exosomes are protected from complement-mediated lysis by expression of CD55 and CD59
    Aled Clayton
    Section of Clinical Oncology and Palliative Medicine, Department of Medicine, University of Wales College of Medicine, Velindre Hospital, Whitchurch, Cardiff, GB
    Eur J Immunol 33:522-31. 2003
    ..We conclude that exosomes express GPI-anchored complement regulators in order to permit their survival in the extracellular environment...
  15. doi request reprint Upregulating CD59: a new strategy for protection of neurons from complement-mediated degeneration
    M V Kolev
    Department of Medical Biochemistry and Immunology, School of Medicine, Cardiff University, Cardiff, UK
    Pharmacogenomics J 10:12-9. 2010
    ..This study brings new insight into immunopharmacological research that may serve to inhibit neuronal death triggered by the terminal pathway of complement activation...
  16. pmc The lipopolysaccharide co-receptor CD14 is present and functional in seminal plasma and expressed on spermatozoa
    C L Harris
    Department of Medical Biochemistry, University of Wales College of Medicine, Cardiff, UK
    Immunology 104:317-23. 2001
    ..Purified spCD14 mediated activation by LPS of CD14-negative cells. These findings suggest that CD14 may play a hitherto unexplored role in immune defence and cell activation in the male reproductive tract...
  17. ncbi request reprint ''Homologous restriction'' in complement lysis: roles of membrane complement regulators
    B Paul Morgan
    Complement Biology Group, Department of Medical Biochemistry and Immunology, School of Medicine, Cardiff University, Cardiff, UK
    Xenotransplantation 12:258-65. 2005
    ..Acceptance of the fact that species selectivity is not a limitation will open new avenues for protection of the xenograft from complement damage...
  18. ncbi request reprint Complement: central to innate immunity and bridging to adaptive responses
    B Paul Morgan
    Department of Medical Biochemistry and Immunology, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UK
    Immunol Lett 97:171-9. 2005
    ..This review will describe the current state of knowledge in this swiftly moving field. It is hoped that the recognition of these properties will help to establish complement in the role it richly deserves as the lynchpin of immunity...
  19. ncbi request reprint Expression of CD46 in developing rat spermatozoa: ultrastructural localization and utility as a marker of the various stages of the seminiferous tubuli
    Masashi Mizuno
    Complement Biology Group, Department of Medical Biochemistry and Immunology, School of Medicine, Cardiff University, Henry Wellcome Building, Heath Park, Cardiff, CF14 4XN, United Kingdom
    Biol Reprod 72:908-15. 2005
    ..Furthermore, this information will aid ongoing studies regarding the roles of CD46 in acrosome-related spermatozoal functions...
  20. ncbi request reprint CD59a is the primary regulator of membrane attack complex assembly in the mouse
    Sivasankar Baalasubramanian
    Complement Biology Group, Department of Medical Biochemistry and Immunology, University of Wales College of Medicine, Cardiff, United Kingdom
    J Immunol 173:3684-92. 2004
    ..Erythrocyte CD59b did not inhibit complement lysis except when CD59a was absent or blocked. These data confirm that CD59a is the primary regulator of complement membrane attack in mouse...
  21. ncbi request reprint Rat membrane cofactor protein (MCP; CD46) is expressed only in the acrosome of developing and mature spermatozoa and mediates binding to immobilized activated C3
    Masashi Mizuno
    Complement Biology Group, Department of Medical Biochemistry and Immunology, University of Wales College of Medicine, Cardiff CF14 4XN, United Kingdom
    Biol Reprod 71:1374-83. 2004
    ..The reagents and results described here will enable studies of the role of spermatozoal MCP in sperm-egg interaction using a relevant animal model system...
  22. doi request reprint Measurement of factor H variants in plasma using variant-specific monoclonal antibodies: application to assessing risk of age-related macular degeneration
    Svetlana Hakobyan
    Department of Medical Biochemistry and Immunology, School of Medicine, Cardiff University, Heath Park Way, Cardiff, United Kingdom
    Invest Ophthalmol Vis Sci 49:1983-90. 2008
    ..This knowledge requires genetic analysis, which is unavailable in most laboratories and which provides no information about the levels of fH protein, a putative linked determinant of disease risk...
  23. ncbi request reprint Complement therapeutics; history and current progress
    B Paul Morgan
    Department of Medical Biochemistry and Immunology, UWCM, Heath Park, Cardiff CF14 4XN, Wales, UK
    Mol Immunol 40:159-70. 2003
    ....
  24. pmc Complement membrane attack is required for endplate damage and clinical disease in passive experimental myasthenia gravis in Lewis rats
    J Chamberlain-Banoub
    Complement Biology Group, Department of Medical Biochemistry and Immunology, School of Medicine, Cardiff University, Cardiff, UK
    Clin Exp Immunol 146:278-86. 2006
    ..The data confirm the essential role of the MAC in the destruction of the endplate in EAMG and raise the prospect of specific MAC inhibition as an alternative therapy in MG patients resistant to conventional treatments...
  25. ncbi request reprint CD55 in rat male reproductive tissue: differential expression in testis and expression of a unique truncated isoform on spermatozoa
    Masashi Mizuno
    Complement Biology Group, Department of Medical Biochemistry and Immunology, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UK
    Mol Immunol 44:1613-22. 2007
    ....
  26. ncbi request reprint Decay-accelerating factor (CD55) is expressed by neurons in response to chronic but not acute autoimmune central nervous system inflammation associated with complement activation
    Johan van Beek
    Brain Inflammation Immunity Group BIIG, Cardiff University, Cardiff, United Kingdom
    J Immunol 174:2353-65. 2005
    ..We conclude that increased CD55 expression by neurons may represent a key protective signaling mechanism mobilized by brain cells to withstand complement activation and to survive within an inflammatory site...
  27. ncbi request reprint CD46 plays a key role in tailoring innate immune recognition of apoptotic and necrotic cells
    Kristina Elward
    Brain Inflammation and Immunity Group BI2G, Department of Medical Biochemistry and Immunology, School of Medicine, Heath Park Campus, Cardiff University, Cardiff CF14 4XN, United Kingdom
    J Biol Chem 280:36342-54. 2005
    ....
  28. doi request reprint Variation in complement component C1 inhibitor in age-related macular degeneration
    J Gibson
    Genetic Epidemiology and Bioinformatics Group, Human Genetics Division, School of Medicine, University of Southampton, Southampton General Hospital, Southampton, UK
    Immunobiology 217:251-5. 2012
    ..This suggests that genetic variation in the promoter region of the SERPING1 gene may influence expression of the gene...
  29. pmc Systemic complement profiling in multiple sclerosis as a biomarker of disease state
    G Ingram
    Department of Neurology, University Hospital of Wales, Cardiff, UK
    Mult Scler 18:1401-11. 2012
    ..There is increasing evidence of significant and dynamic systemic activation and upregulation of complement in multiple sclerosis (MS), which may contribute to disease pathogenesis...
  30. pmc Molecular and functional analysis of mouse decay accelerating factor (CD55)
    C L Harris
    Department of Medical Biochemistry, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XN, U K
    Biochem J 341:821-9. 1999
    ..Transfection with DAF also conferred protection from complement in a cell-lysis assay, and a soluble, recombinant form of mouse DAF inhibited complement in a haemolytic assay...
  31. pmc Identification of the high affinity binding site in the Streptococcus intermedius toxin intermedilysin for its membrane receptor, the human complement regulator CD59
    Timothy R Hughes
    Complement Biology Group, Department of Medical Biochemistry and Immunology, School of Medicine, Cardiff University, Cardiff CF14 4XN, United Kingdom
    Mol Immunol 46:1561-7. 2009
    ..The demonstration that a peptide modeled on the CD59-binding site inhibits intermedilysin-mediated haemolysis leads us to suggest that such peptides might be useful in treating infections caused by intermedilysin-producing bacteria...
  32. ncbi request reprint Complement and complement regulators in the male reproductive system
    Claire L Harris
    Complement Biology Group, Department of Medical Biochemistry and Immunology, School of Medicine, Cardiff University, Health Park, Cardiff CF 14 4XN, UK
    Mol Immunol 43:57-67. 2006
    ..An understanding of these roles will inform studies of their contribution to fertility and infertility in man...
  33. pmc Bacterial expression and membrane targeting of the rat complement regulator Crry: a new model anticomplement therapeutic
    Deborah A Fraser
    Department of Medical Biochemistry, University of Wales College of Medicine, Cardiff CF14 4XX, UK
    Protein Sci 11:2512-21. 2002
    ..This novel anticomplement agent can now be applied to rat models of arthritis and other inflammatory diseases...
  34. ncbi request reprint Generation of a recombinant, membrane-targeted form of the complement regulator CD59: activity in vitro and in vivo
    Deborah A Fraser
    Departments of Medical Biochemistry and Rheumatology, University of Wales College of Medicine, Heath Park, Cardiff, United Kingdom
    J Biol Chem 278:48921-7. 2003
    ..This novel type of agent, termed sCD59-APT542, offers for the first time the prospect of efficient and specific inhibition of membrane attack complex activity in vivo...
  35. ncbi request reprint In vivo characterization and therapeutic efficacy of a C5-specific inhibitor from the soft tick Ornithodoros moubata
    Natalie J Hepburn
    Department of Medical Biochemistry and Immunology, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, United Kingdom
    J Biol Chem 282:8292-9. 2007
    ..These data offer exciting prospects for targeted treatment of complement-mediated diseases without the detrimental inhibition of the opsonic roles of complement...
  36. ncbi request reprint Holding T cells in check--a new role for complement regulators?
    M Paula Longhi
    Medical Biochemistry and Immunology, Henry Wellcome Building, Cardiff University, Heath Park, Cardiff CF14 4XN, Wales, UK
    Trends Immunol 27:102-8. 2006
    ..Here we review these studies, which reveal another important link between the innate and the adaptive immune system...
  37. ncbi request reprint Decay-accelerating factor must bind both components of the complement alternative pathway C3 convertase to mediate efficient decay
    Claire L Harris
    Department of Medical Biochemistry and Immunology, School of Medicine, Cardiff University, Cardiff, United Kingdom
    J Immunol 178:352-9. 2007
    ..These data provide new insights into the mechanisms involved in C3 convertase decay by DAF...
  38. ncbi request reprint Complement, roles in renal disease and modulation for therapy
    N J Hepburn
    Department of Medical Biochemistry and Immunology, School of Medicine, Cardiff University, Heath Park, Cardiff, UK
    Clin Nephrol 70:357-76. 2008
    ....
  39. ncbi request reprint Immunization with autologous CD46 generates a strong autoantibody response in rats that targets spermatozoa
    Masashi Mizuno
    Complement Biology Group, Department of Medical Biochemistry and Immunology, School of Medicine, Cardiff University, Henry Wellcome Building, Heath Park, Cardiff, UK
    J Reprod Immunol 73:135-47. 2007
    ..The anti-CD46 response did not influence fertility, perhaps reflecting the considerable redundancy for fertilization in rodents...
  40. ncbi request reprint Spermatogenic cells distal to the blood-testis barrier in rats lack C3 convertase regulators and may be at risk of complement-mediated injury
    Masashi Mizuno
    Complement Biology Group, Department of Medical Biochemistry and Immunology, School of Medicine, Cardiff University, Henry Wellcome Building, Heath Park, Cardiff CF14 4XN, UK
    J Reprod Immunol 69:23-34. 2006
    ....
  41. pmc Glycation of CD59 impairs complement regulation on erythrocytes from diabetic subjects
    Catherine S Davies
    Department of Medical Biochemistry and Immunology, School of Medicine, Cardiff University, Cardiff, UK
    Immunology 114:280-6. 2005
    ..These data implicate glycation-induced inactivation of CD59 as a factor contributing to anaemia in type 1 diabetes...
  42. doi request reprint Crry deficiency in complement sufficient mice: C3 consumption occurs without associated renal injury
    Marieta M Ruseva
    Complement Biology Group, Department of Medical Biochemistry and Immunology, School of Medicine, Cardiff University, Cardiff CF14 4XN, UK
    Mol Immunol 46:803-11. 2009
    ....
  43. pmc Complement factor H binds to denatured rather than to native pentameric C-reactive protein
    Svetlana Hakobyan
    Department of Medical Biochemistry and Immunology and Pharmacology, School of Medicine, Cardiff University, Cardiff CF14 4XN, UK
    J Biol Chem 283:30451-60. 2008
    ..Differential binding to C-reactive protein, thus, does not explain association of the Y402H polymorphism with macular degeneration...
  44. pmc Decay-accelerating factor induction by tumour necrosis factor-alpha, through a phosphatidylinositol-3 kinase and protein kinase C-dependent pathway, protects murine vascular endothelial cells against complement deposition
    Saifur R Ahmad
    British Heart Foundation Cardiovascular Medicine Unit, The Bywaters Centre, Imperial College London, Hammersmith Hospital, London, UK
    Immunology 110:258-68. 2003
    ..The mouse represents a suitable model for the study of novel therapeutic approaches by which vascular endothelium may be conditioned against complement-mediated injury...
  45. ncbi request reprint CD59, a complement regulatory protein, controls choroidal neovascularization in a mouse model of wet-type age-related macular degeneration
    Nalini S Bora
    Department of Ophthalmology, Jones Eye Institute, Pat and Willard Walker Eye Research Center, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA
    J Immunol 178:1783-90. 2007
    ..Thus, our results suggest that the inhibition of complement by soluble CD59 may provide a novel therapeutic alternative to current treatment...
  46. pmc Combined yeast {beta}-glucan and antitumor monoclonal antibody therapy requires C5a-mediated neutrophil chemotaxis via regulation of decay-accelerating factor CD55
    Bing Li
    Tumor Immunobiology Program of the James Graham Brown Cancer Center, Department of Medicine, University of Louisville School of Medicine, Louisville, Kentucky 40202, USA
    Cancer Res 67:7421-30. 2007
    ..These results suggest a critical role for CD55 to regulate iC3b and C5a release and in turn to influence the recruitment of beta-glucan-primed neutrophils eliciting killing activity...
  47. pmc Gain-of-function mutations in complement factor B are associated with atypical hemolytic uremic syndrome
    Elena Goicoechea de Jorge
    Centro de Investigaciones Biologicas, Consejo Superior de Investigaciones Cientificas, Ramiro de Maeztu 9, 28040 Madrid, Spain
    Proc Natl Acad Sci U S A 104:240-5. 2007
    ....
  48. ncbi request reprint Increased susceptibility to complement attack due to down-regulation of decay-accelerating factor/CD55 in dysferlin-deficient muscular dystrophy
    Katrin Wenzel
    Myology Research Group, Department of Neurology, Charite University Hospital, Berlin, Germany
    J Immunol 175:6219-25. 2005
    ..In conclusion, a novel mechanism of muscle fiber injury in dysferlin-deficient muscular dystrophy is demonstrated, possibly opening therapeutic avenues in this to date untreatable disorder...