Fiona M Watt

Summary

Affiliation: Cancer Research UK
Country: UK

Publications

  1. ncbi Women in cell biology: how personal lives shape careers
    Fiona M Watt
    Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Nat Rev Mol Cell Biol 7:378-80. 2006
  2. ncbi Women in cell biology: getting to the top
    Fiona M Watt
    Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Nat Rev Mol Cell Biol 7:287-90. 2006
  3. ncbi Epidermal stem cells: an update
    Fiona M Watt
    Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London, WC2A 3PX, UK
    Curr Opin Genet Dev 16:518-24. 2006
  4. ncbi Jagged 1 is a beta-catenin target gene required for ectopic hair follicle formation in adult epidermis
    Soline Estrach
    Keratinocyte Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Development 133:4427-38. 2006
  5. ncbi Identification of novel keratinocyte differentiation modulating compounds by high-throughput screening
    Masaru Honma
    Keratinocyte Laboratory, Cancer Research UK London Research Institute, London
    J Biomol Screen 11:977-84. 2006
  6. pmc The basement membrane of hair follicle stem cells is a muscle cell niche
    Hironobu Fujiwara
    Cancer Research UK Cambridge Research Institute, Li Ka Shing Centre, UK
    Cell 144:577-89. 2011
  7. pmc The therapeutic potential of stem cells
    Fiona M Watt
    Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Cambridge, UK
    Philos Trans R Soc Lond B Biol Sci 365:155-63. 2010
  8. pmc MYC in mammalian epidermis: how can an oncogene stimulate differentiation?
    Fiona M Watt
    Cancer Research UK Cambridge Research Institute, Li Ka Shing Centre, Robinson Way, Cambridge CB2 0RE, UK
    Nat Rev Cancer 8:234-42. 2008
  9. pmc Epidermal Notch signalling: differentiation, cancer and adhesion
    Fiona M Watt
    Cancer Research UK Cambridge Research Institute, Li Ka Shing Centre, Robinson Way, Cambridge CB2 0RE, UK
    Curr Opin Cell Biol 20:171-9. 2008
  10. pmc Epidermal stem cell diversity and quiescence
    Fiona M Watt
    Wellcome Trust Centre for Stem Cell Research, Tennis Court Road, Cambridge, UK
    EMBO Mol Med 1:260-7. 2009

Detail Information

Publications97

  1. ncbi Women in cell biology: how personal lives shape careers
    Fiona M Watt
    Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Nat Rev Mol Cell Biol 7:378-80. 2006
    ..Growing up, some scientists enjoyed parental support, whereas for others low expectations or a geographical disadvantage were a spur to achievement. Partners can be good, bad or indifferent, but having children is not a bar to success...
  2. ncbi Women in cell biology: getting to the top
    Fiona M Watt
    Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Nat Rev Mol Cell Biol 7:287-90. 2006
    ..By contrast, my approach has been to focus on individual women who have 'made it' in science and to ask how their careers have been affected by their sex. Some positive conclusions emerge, but the overall picture is disheartening...
  3. ncbi Epidermal stem cells: an update
    Fiona M Watt
    Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London, WC2A 3PX, UK
    Curr Opin Genet Dev 16:518-24. 2006
    ..This work has also yielded new insights into skin tumour development...
  4. ncbi Jagged 1 is a beta-catenin target gene required for ectopic hair follicle formation in adult epidermis
    Soline Estrach
    Keratinocyte Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Development 133:4427-38. 2006
    ..beta-catenin stimulates Notch signalling by inducing Jag1 transcription. We conclude that the Notch pathway acts downstream of the Wnt/beta-catenin pathway to determine epidermal cell fate...
  5. ncbi Identification of novel keratinocyte differentiation modulating compounds by high-throughput screening
    Masaru Honma
    Keratinocyte Laboratory, Cancer Research UK London Research Institute, London
    J Biomol Screen 11:977-84. 2006
    ..Because the compound affected the tumor cells at a lower concentration than primary keratinocytes, it may have potential as an antitumor therapy...
  6. pmc The basement membrane of hair follicle stem cells is a muscle cell niche
    Hironobu Fujiwara
    Cancer Research UK Cambridge Research Institute, Li Ka Shing Centre, UK
    Cell 144:577-89. 2011
    ..Thus, bulge stem cells, via nephronectin expression, create a smooth muscle cell niche and act as tendon cells for the APM. Our results reveal a functional role for basement membrane heterogeneity in tissue patterning. PAPERCLIP:..
  7. pmc The therapeutic potential of stem cells
    Fiona M Watt
    Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Cambridge, UK
    Philos Trans R Soc Lond B Biol Sci 365:155-63. 2010
    ..What makes stem cell research so exciting is its tremendous potential to benefit human health and the opportunities for interdisciplinary research that it presents...
  8. pmc MYC in mammalian epidermis: how can an oncogene stimulate differentiation?
    Fiona M Watt
    Cancer Research UK Cambridge Research Institute, Li Ka Shing Centre, Robinson Way, Cambridge CB2 0RE, UK
    Nat Rev Cancer 8:234-42. 2008
    ....
  9. pmc Epidermal Notch signalling: differentiation, cancer and adhesion
    Fiona M Watt
    Cancer Research UK Cambridge Research Institute, Li Ka Shing Centre, Robinson Way, Cambridge CB2 0RE, UK
    Curr Opin Cell Biol 20:171-9. 2008
    ..The effects on differentiation can be cell autonomous and non-autonomous, and Notch contributes to stem cell clustering via modulation of cell adhesion...
  10. pmc Epidermal stem cell diversity and quiescence
    Fiona M Watt
    Wellcome Trust Centre for Stem Cell Research, Tennis Court Road, Cambridge, UK
    EMBO Mol Med 1:260-7. 2009
    ..The stage is now set for exploring the relationship between the different epidermal stem cell populations and between quiescence and lineage selection...
  11. pmc Role of integrins in regulating epidermal adhesion, growth and differentiation
    Fiona M Watt
    Cancer Research UK, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    EMBO J 21:3919-26. 2002
    ..In this review I summarize what is known about the expression and function of epidermal integrins, and highlight the challenges for future research...
  12. ncbi The stem cell compartment in human interfollicular epidermis
    Fiona M Watt
    Imperial Cancer Research Fund, London, UK
    J Dermatol Sci 28:173-80. 2002
    ..beta1 integrins, Notch, c-Myc and beta-catenin all regulate the size of the stem cell compartment in vitro and recent evidence from transgenic mice suggests that they are also important in vivo...
  13. pmc Sox2 modulates the function of two distinct cell lineages in mouse skin
    Marta H Lesko
    Wellcome Trust Medical Research Council Centre for Stem Cell Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK Centre for Stem Cells and Regenerative Medicine, King s College London, 28th Floor, Guy s Tower, London SE1 9RT, UK
    Dev Biol 382:15-26. 2013
    ..We conclude that Sox2 is a marker of two distinct lineages in the skin and regulates the number of differentiated cells in the case of the Merkel cell lineage and hair follicle type in the case of the DP...
  14. pmc The RNA-methyltransferase Misu (NSun2) poises epidermal stem cells to differentiate
    Sandra Blanco
    Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Cambridge, United Kingdom
    PLoS Genet 7:e1002403. 2011
    ..Our results reveal that post-transcriptional RNA methylation can play a previously unappreciated role in controlling stem cell fate...
  15. pmc Lrig1 expression defines a distinct multipotent stem cell population in mammalian epidermis
    Kim B Jensen
    Laboratory for Epidermal Stem Cell Biology, Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Cambridge, UK
    Cell Stem Cell 4:427-39. 2009
    ..Our results suggest that Lrig1-positive cells constitute a previously unidentified reservoir of adult mouse interfollicular epidermal stem cells...
  16. pmc Necl2 regulates epidermal adhesion and wound repair
    Adam Giangreco
    Cancer Research UK Cambridge Research Institute, Li Ka Shing Centre, Cambridge, UK
    Development 136:3505-14. 2009
    ..Our results demonstrate that Necl2 is involved in regulating epidermal stem cell quiescence and location...
  17. ncbi Role of the Notch ligand Delta1 in embryonic and adult mouse epidermis
    Soline Estrach
    Wellcome Trust Centre for Stem Cell Research, Cambridge, UK
    J Invest Dermatol 128:825-32. 2008
    ..These results demonstrate that Dll1 contributes to the control of proliferation and differentiation in IFE, whereas Jagged1 regulates hair follicle differentiation...
  18. pmc An activating beta1 integrin mutation increases the conversion of benign to malignant skin tumors
    Manuela Ferreira
    Cancer Research UK Cambridge Research Institute, Cambridge, United Kingdom
    Cancer Res 69:1334-42. 2009
    ..These observations establish that the expression of a genetic variant in the I-like domain of beta1 integrins does not affect normal epidermal homeostasis, but increases tumor susceptibility and influences tumor type...
  19. doi Kazrin regulates keratinocyte cytoskeletal networks, intercellular junctions and differentiation
    Lisa M Sevilla
    Epithelial Cell Biology Laboratory, Cancer Research UK Cambridge Research Institute, Li Ka Shing Centre, Robinson Way, Cambridge CB2 0RE, UK
    J Cell Sci 121:3561-9. 2008
    ..We conclude that kazrin is a dual regulator of intercellular adhesion and differentiation in keratinocytes and regulates these processes by Rho-dependent and -independent mechanisms...
  20. ncbi Beta-catenin and Hedgehog signal strength can specify number and location of hair follicles in adult epidermis without recruitment of bulge stem cells
    Violeta Silva-Vargas
    Keratinocyte Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
    Dev Cell 9:121-31. 2005
    ....
  21. ncbi Stem cell depletion through epidermal deletion of Rac1
    Salvador Aznar Benitah
    Keratinocyte Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Science 309:933-5. 2005
    ..We conclude that a pleiotropic regulator of cell adhesion and the cytoskeleton plays a critical role in controlling exit from the stem cell niche and propose that Rac and Myc represent a global stem cell regulatory axis...
  22. pmc KazrinE is a desmosome-associated liprin that colocalises with acetylated microtubules
    Rachida Nachat
    Cancer Research UK Cambridge Research Institute, Li Ka Shing Centre, Cambridge, CB2 0RE, UK
    J Cell Sci 122:4035-41. 2009
    ..We speculate that the kazrinE-microtubule interaction contributes to the mechanism by which kazrin regulates desmosome formation and epidermal differentiation...
  23. pmc Adult epidermal Notch activity induces dermal accumulation of T cells and neural crest derivatives through upregulation of jagged 1
    Carrie A Ambler
    Cancer Research UK, Cambridge Research Institute, Li Ka Shing Centre, Cambridge CB2 0RE, UK
    Development 137:3569-79. 2010
    ..We conclude that jagged 1 is a key mediator of non-cell autonomous Notch signalling in skin...
  24. ncbi Transient activation of FOXN1 in keratinocytes induces a transcriptional programme that promotes terminal differentiation: contrasting roles of FOXN1 and Akt
    Sam M Janes
    Keratinocyte Laboratory, Cancer Research UK, London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    J Cell Sci 117:4157-68. 2004
    ..These results establish a role for FOXN1 in initiation of terminal differentiation and implicate Akt in subsequent events...
  25. pmc Single-cell gene expression profiling reveals functional heterogeneity of undifferentiated human epidermal cells
    David W M Tan
    Epidermal Stem Cell Biology Laboratory, Wellcome Trust Medical Research Council Cambridge Stem Cell Institute, Tennis Court Road, Cambridge, CB2 1QR, UK
    Development 140:1433-44. 2013
    ..Thus, the previously unknown heterogeneity revealed by our studies results in differences in the interaction of undifferentiated basal keratinocytes with their environment...
  26. pmc Exons 5-15 of kazrin are dispensable for murine epidermal morphogenesis and homeostasis
    Mariya K Chhatriwala
    Wellcome Trust Centre for Stem Cell Research, Cambridge University, Cambridge, UK
    J Invest Dermatol 132:1977-87. 2012
    ..The previously reported role of kazrin in regulating cell shape appears to reside within the N-terminal coiled-coil domain encoded by exons 1-4...
  27. doi Diverse epigenetic strategies interact to control epidermal differentiation
    Klaas W Mulder
    Epithelial Cell Biology Group, Cancer Research UK Cambridge Research Institute, Robinson Way, Cambridge CB2 0RE, UK
    Nat Cell Biol 14:753-63. 2012
    ..Thus, regulation of distinct gene expression programs through the interplay between diverse epigenetic strategies protects epidermal stem cells from differentiation...
  28. doi Dynamic regulation of retinoic acid-binding proteins in developing, adult and neoplastic skin reveals roles for beta-catenin and Notch signalling
    Charlotte A Collins
    Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Dev Biol 324:55-67. 2008
    ..Our findings demonstrate that there is dynamic regulation of RA signalling in different regions of the skin and provide evidence for interactions between the RA, beta-catenin and Notch pathways...
  29. pmc Sox2-positive dermal papilla cells specify hair follicle type in mammalian epidermis
    Ryan R Driskell
    Wellcome Trust Centre for Stem Cell Research, Cambridge, UK
    Development 136:2815-23. 2009
    ..We have thus demonstrated a previously unrecognised heterogeneity in dermal papilla cells and shown that Sox2-positive cells specify particular hair follicle types...
  30. pmc A stem cell gene expression profile of human squamous cell carcinomas
    Kim B Jensen
    Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QR, United Kingdom
    Cancer Lett 272:23-31. 2008
    ..We conclude that SCCs do not reflect a simple expansion of stem cells; rather, tumour cells hijack the homeostatic controls that operate in normal stem cells, eliminating those that maintain stem cell quiescence...
  31. pmc Tumor formation initiated by nondividing epidermal cells via an inflammatory infiltrate
    Esther N Arwert
    Cancer Research UK Cambridge Research Institute, Cambridge CB2 0RE, United Kingdom
    Proc Natl Acad Sci U S A 107:19903-8. 2010
    ..In contrast, our studies show that differentiated epidermal cells can initiate tumor formation without reacquiring the ability to divide and that they do so by triggering an inflammatory infiltrate...
  32. ncbi HAN11 binds mDia1 and controls GLI1 transcriptional activity
    Kazumasa Morita
    Keratinocyte Laboratory, Cancer Research UK London Research Institute, London WC2A 3PX, England, UK
    J Dermatol Sci 44:11-20. 2006
    ..GLI1-dependent transcription is positively regulated by DYRK1A, which is reported to bind HAN11. HAN11 is the human homologue of AN11, which controls flavonoid synthesis in plants...
  33. ncbi Manipulation of stem cell proliferation and lineage commitment: visualisation of label-retaining cells in wholemounts of mouse epidermis
    Kristin M Braun
    Keratinocyte Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Development 130:5241-55. 2003
    ..We conclude that LRC are more sensitive to some proliferative stimuli than others and that changes in lineage can occur with or without recruitment of LRC into cycle...
  34. ncbi Role of melanoma chondroitin sulphate proteoglycan in patterning stem cells in human interfollicular epidermis
    James Legg
    Keratinocyte Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London, WC2A 3PX, UK
    Development 130:6049-63. 2003
    ..We conclude that MCSP is a novel marker for epidermal stem cells that contributes to their patterned distribution by promoting stem cell clustering...
  35. pmc Mice deficient in involucrin, envoplakin, and periplakin have a defective epidermal barrier
    Lisa M Sevilla
    Cancer Research UK, Cambridge Research Institute, Li Ka Shing Centre, Cambridge CB2 0RE, England, UK
    J Cell Biol 179:1599-612. 2007
    ..Thus, combined loss of the cornified envelope proteins not only impairs the epidermal barrier, but also changes the composition of T cell subpopulations in the skin...
  36. pmc Syntenin mediates Delta1-induced cohesiveness of epidermal stem cells in culture
    Soline Estrach
    Wellcome Trust Centre for Stem Cell Research, Tennis Court Road, Cambridge, CB2 1QR, UK
    J Cell Sci 120:2944-52. 2007
    ..We propose that syntenin binding to Delta1 plays a dual role in promoting intercellular adhesion and regulating Notch signalling...
  37. pmc PI3-kinase-dependent activation of apoptotic machinery occurs on commitment of epidermal keratinocytes to terminal differentiation
    Sam M Janes
    Centre for Respiratory Research, Rayne Institute, University College London, 5 University Street, London, UK
    Cell Res 19:328-39. 2009
    ..These properties indicate that the onset of terminal differentiation, while regulated by PI3-kinase and caspases, is not a classical apoptotic process...
  38. ncbi Expression of activated MEK1 in differentiating epidermal cells is sufficient to generate hyperproliferative and inflammatory skin lesions
    Robin M Hobbs
    Keratinocyte Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    J Invest Dermatol 123:503-15. 2004
    ..These data suggest that activation of MEK1 downstream of beta1 integrins plays an important role in epidermal hyperproliferation and skin inflammation...
  39. ncbi Dual role of inactivating Lef1 mutations in epidermis: tumor promotion and specification of tumor type
    Catherin Niemann
    Cancer Research UK London Research Institute, London, United Kingdom
    Cancer Res 67:2916-21. 2007
    ..We propose that LEF1 NH(2)-terminal mutations play a dual role in skin cancer, specifying tumor type by inhibiting Wnt signaling and acting as a tumor promoter by preventing induction of p53...
  40. ncbi Topo II alpha reporter mice reveal proliferative regions in the epidermis and small intestine
    Elizabeth R Li
    Keratinocyte Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
    FEBS Lett 579:6479-85. 2005
    ..The mice therefore provide a useful model for non-invasive imaging of regions of high proliferation in normal or damaged epidermis...
  41. doi Epidermal Cadm1 expression promotes autoimmune alopecia via enhanced T cell adhesion and cytotoxicity
    Adam Giangreco
    Cancer Research UK Cambridge Research Institute, Cambridge CB2 0RE, United Kingdom
    J Immunol 188:1514-22. 2012
    ..Our findings reveal that Cadm1 expression in the hair follicle plays a role in autoimmune alopecia...
  42. pmc Epidermal stem cells are defined by global histone modifications that are altered by Myc-induced differentiation
    Michaela Frye
    Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Cambridge, United Kingdom
    PLoS ONE 2:e763. 2007
    ..We conclude that Myc-induced chromatin modifications play a major role in Myc-induced exit from the stem cell compartment...
  43. pmc Dose and context dependent effects of Myc on epidermal stem cell proliferation and differentiation
    Melanie A Berta
    CR UK Cambridge Research Institute, Li Ka Shing Centre, Cambridge CB2 0RE, UK
    EMBO Mol Med 2:16-25. 2010
    ..We conclude that Myc induced differentiation acts as a fail-safe device to prevent uncontrolled proliferation and neoplastic conversion of epidermal stem cells expressing high levels of Myc...
  44. ncbi The RNA methyltransferase Misu (NSun2) mediates Myc-induced proliferation and is upregulated in tumors
    Michaela Frye
    Keratinocyte Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
    Curr Biol 16:971-81. 2006
    ..In adult epidermis, Myc stimulates exit from the stem cell compartment, decreasing cell adhesion and, by an unknown mechanism, triggering proliferation of transit-amplifying cells...
  45. ncbi Suprabasal alpha6beta4 integrin expression in epidermis results in enhanced tumourigenesis and disruption of TGFbeta signalling
    David M Owens
    Keratinocyte Laboratory, CR UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    J Cell Sci 116:3783-91. 2003
    ..These data suggest that suprabasal alpha6beta4 promotes tumourigenesis by preventing TGFbeta from suppressing clonal expansion of initiated cells in the epidermal basal layer...
  46. pmc LRIG1 regulates cadherin-dependent contact inhibition directing epithelial homeostasis and pre-invasive squamous cell carcinoma development
    Liwen Lu
    Lungs for Living Research Centre, UCL Respiratory, University College London, 5 University Street, London, WC1E 6JF, UK
    J Pathol 229:608-20. 2013
    ..Our findings imply that the early stages of squamous carcinoma development are driven by a change in amplitude of EGFR signalling governed by the loss of contact inhibition...
  47. pmc Polyclonal origin and hair induction ability of dermal papillae in neonatal and adult mouse back skin
    Charlotte A Collins
    Epidermal Stem Cell Laboratory, Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Dev Biol 366:290-7. 2012
    ..We propose that during skin reconstitution fibroblasts may be induced to become DP cells by interactions with hair follicle epidermal cells, rather than being derived from a distinct subpopulation of cells...
  48. pmc Clonal growth of dermal papilla cells in hydrogels reveals intrinsic differences between Sox2-positive and -negative cells in vitro and in vivo
    Ryan R Driskell
    Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Cambridge, UK
    J Invest Dermatol 132:1084-93. 2012
    ..As the culture system supports clonal growth of DP cells and maintenance of distinct DP cell types, it will be useful for further analysis of intrinsic and extrinsic signals controlling DP function...
  49. doi Actin and serum response factor transduce physical cues from the microenvironment to regulate epidermal stem cell fate decisions
    John T Connelly
    Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QR, UK
    Nat Cell Biol 12:711-8. 2010
    ..Our findings demonstrate how biophysical cues are transduced into transcriptional responses that determine epidermal cell fate...
  50. ncbi Different consequences of beta1 integrin deletion in neonatal and adult mouse epidermis reveal a context-dependent role of integrins in regulating proliferation, differentiation, and intercellular communication
    Teresa Lopez-Rovira
    Keratinocyte Laboratory, Cancer Research UK London Research Institute, London, UK
    J Invest Dermatol 125:1215-27. 2005
    ..Our results highlight the context-dependent effects of beta1 integrin deletion and suggest that inflammation may be responsible for some of the K5Cre beta1-null phenotype...
  51. pmc Distinct fibroblast lineages determine dermal architecture in skin development and repair
    Ryan R Driskell
    1 Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Cambridge CB2 1QR, UK 2 Centre for Stem Cells and Regenerative Medicine, King s College London, 28th Floor, Tower Wing, Guy s Hospital, London SE1 9RT, UK
    Nature 504:277-81. 2013
    ..They also form a platform for discovering fibroblast lineages in other tissues and for examining fibroblast changes in ageing and disease. ..
  52. pmc Mimicking normal tissue architecture and perturbation in cancer with engineered micro-epidermis
    Julien E Gautrot
    Melville Laboratory for Polymer Synthesis, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK
    Biomaterials 33:5221-9. 2012
    ..The micro-epidermis we have generated provides a new platform for screening drugs that modulate tissue assembly, quantifying tissue stratification and investigating the properties of tumour cells...
  53. pmc Differential sensitivity of epidermal cell subpopulations to beta-catenin-induced ectopic hair follicle formation
    Christopher M Baker
    CRUK Cambridge Research Institute, Li Ka Shing Centre, Robinson Way, Cambridge CB2 0RE, UK
    Dev Biol 343:40-50. 2010
    ..Our results suggest that the bulge and sebaceous gland are, respectively, non-permissive and permissive niches for Wnt induced hair follicle differentiation...
  54. doi KazrinA is required for axial elongation and epidermal integrity in Xenopus tropicalis
    Lisa M Sevilla
    Epithelial Cell Biology Laboratory, Cancer Research UK Cambridge Research Institute, Li Ka Shing Centre, Cambridge, United Kingdom
    Dev Dyn 237:1718-25. 2008
    ..Together, our results suggest that loss of kazrinA causes defects in cell adhesion that affect axial elongation, cell differentiation, and epidermal morphogenesis...
  55. pmc Epidermal stem cells are retained in vivo throughout skin aging
    Adam Giangreco
    Cancer Research UK, Cambridge Research Institute, Li Ka Shing Centre, Robinson Way, Cambridge CB2 0RE, UK
    Aging Cell 7:250-9. 2008
    ..These findings, coupled with observed dermal gene expression changes, suggest that epidermal stem cells themselves are intrinsically aging resistant and that local environmental or systemic factors modulate skin aging...
  56. ncbi Evidence that Myc activation depletes the epidermal stem cell compartment by modulating adhesive interactions with the local microenvironment
    Michaela Frye
    Keratinocyte Laboratory, Cancer Research UK, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Development 130:2793-808. 2003
    ....
  57. pmc Reprogramming adult dermis to a neonatal state through epidermal activation of β-catenin
    Charlotte A Collins
    Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Development 138:5189-99. 2011
    ..Our results show that adult dermis is an unexpectedly plastic tissue that can be reprogrammed to acquire the molecular, cellular and structural characteristics of neonatal dermis in response to cues from the overlying epidermis...
  58. pmc Role of LIM kinases in normal and psoriatic human epidermis
    Masaru Honma
    Keratinocyte Laboratory, Cancer Research UK London Research Institute, London WC2A 3PX, United Kingdom
    Mol Biol Cell 17:1888-96. 2006
    ....
  59. pmc c-MYC-induced sebaceous gland differentiation is controlled by an androgen receptor/p53 axis
    Denny L Cottle
    Wellcome Trust Medical Research Council Stem Cell Institute SCI, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Cell Rep 3:427-41. 2013
    ..Thus, the consequences of overactivating MYC in the SG depend on whether AR or p53 is activated, as they form a regulatory axis controlling proliferation and differentiation...
  60. pmc FRMD4A upregulation in human squamous cell carcinoma promotes tumor growth and metastasis and is associated with poor prognosis
    Stephen J Goldie
    CRUK Cambridge Research Institute, Li Ka Shing Centre, Cambridge, United Kingdom
    Cancer Res 72:3424-36. 2012
    ..Together, our findings suggest FRMD4A as a novel candidate therapeutic target in HNSCC based on the key role in metastatic growth we have identified...
  61. doi Genomic gain of 5p15 leads to over-expression of Misu (NSUN2) in breast cancer
    Michaela Frye
    Wellcome Trust Centre for Stem Cell Research, Tennis Court Road, Cambridge CB2 1QR, UK
    Cancer Lett 289:71-80. 2010
    ..We conclude that Misu is up-regulated in a substantial proportion of breast cancers and has therapeutic potential as a drug target...
  62. ncbi Contribution of stem cells and differentiated cells to epidermal tumours
    David M Owens
    Cancer Research UK, London Research Institute, Keratinocyte Laboratory, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Nat Rev Cancer 3:444-51. 2003
    ..So, what genetic and environmental factors determine whether a mutant stem cell forms a tumour and what type of tumour will develop?..
  63. ncbi Expression of Notch pathway genes in mammalian epidermis and modulation by beta-catenin
    Carrie A Ambler
    Keratinocyte Laboratory, Cancer Research UK London Research Institute, London, United Kingdom
    Dev Dyn 236:1595-601. 2007
    ..beta-Catenin-induced up-regulation of Jagged1 precedes induction of other Notch target genes. The different sites of expression of Hes and Hey genes suggest input from additional signaling pathways...
  64. pmc Single-cell expression profiling of human epidermal stem and transit-amplifying cells: Lrig1 is a regulator of stem cell quiescence
    Kim B Jensen
    Keratinocyte Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Proc Natl Acad Sci U S A 103:11958-63. 2006
    ..Lrig1 acted in part by negatively regulating the Myc promoter. We propose that Lrig1 maintains epidermal stem cells in a quiescent nondividing state, and that Lrig1 down-regulation triggers proliferation...
  65. doi Extracellular-matrix tethering regulates stem-cell fate
    Britta Trappmann
    Melville Laboratory for Polymer Synthesis, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK
    Nat Mater 11:642-9. 2012
    ..Lower collagen anchoring density resulted in increased differentiation. We conclude that stem cells exert a mechanical force on collagen fibres and gauge the feedback to make cell-fate decisions...
  66. pmc Lrig1 controls intestinal stem-cell homeostasis by negative regulation of ErbB signalling
    Vivian W Y Wong
    The Anne McLaren Laboratory for Regenerative Medicine, University of Cambridge, Cambridge CB2 0SZ, UK
    Nat Cell Biol 14:401-8. 2012
    ..Our findings reveal ErbB activation as a strong inductive signal for stem-cell proliferation. This has implications for our understanding of ErbB signalling in tissue development and maintenance and the progression of malignant disease...
  67. doi Epithelial stem cells, wound healing and cancer
    Esther N Arwert
    Cancer Research UK Cambridge Research Institute, Li Ka Shing Centre, Robinson Way, Cambridge CB2 ORE, UK
    Nat Rev Cancer 12:170-80. 2012
    ..These studies suggest ways to harness stem cells for wound repair while minimizing cancer risk...
  68. pmc Kazrin, a novel periplakin-interacting protein associated with desmosomes and the keratinocyte plasma membrane
    Karen R Groot
    Keratinocyte Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    J Cell Biol 166:653-9. 2004
    ..On transfection, all three kazrin isoforms have similar subcellular distributions. We conclude that kazrin is a novel component of desmosomes that associates with periplakin...
  69. pmc The vitamin D receptor is a Wnt effector that controls hair follicle differentiation and specifies tumor type in adult epidermis
    Hector G Palmer
    Cancer Research UK Cambridge Research Institute, Li Ka Shing Centre, Cambridge, United Kingdom
    PLoS ONE 3:e1483. 2008
    ..We conclude that VDR is a TCF/Lef-independent transcriptional effector of the Wnt pathway and that vitamin D analogues have therapeutic potential in tumors with inappropriate activation of Wnt signalling...
  70. ncbi Transient activation of beta-catenin signalling in adult mouse epidermis is sufficient to induce new hair follicles but continuous activation is required to maintain hair follicle tumours
    Cristina Lo Celso
    Keratinocyte Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Development 131:1787-99. 2004
    ..We conclude that interfollicular epidermis and sebaceous glands retain the ability to be reprogrammed in adult life and that continuous beta-catenin signalling is required to maintain hair follicle tumours...
  71. ncbi New roles for integrins in squamous-cell carcinoma
    Sam M Janes
    Keratinocyte Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Nat Rev Cancer 6:175-83. 2006
    ..These findings provide a mechanistic basis for the well-established links between altered integrin expression and tumour prognosis...
  72. doi Lineage tracing
    Kai Kretzschmar
    Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Cell 148:33-45. 2012
    ..Here we discuss the different experimental strategies currently available for lineage tracing, their associated caveats, and new opportunities to integrate lineage tracing with the monitoring of intracellular signaling pathways...
  73. ncbi Designer skin: lineage commitment in postnatal epidermis
    Catherin Niemann
    Keratinocyte Laboratory, Cancer Research UK, 44 Lincoln s Inn Fields, WC2A 3PX, London, UK
    Trends Cell Biol 12:185-92. 2002
    ..We also present evidence that postnatal epidermis can be reprogrammed, altering the number and location of cells that differentiate along specific epidermal lineages...
  74. pmc Exploiting the superior protein resistance of polymer brushes to control single cell adhesion and polarisation at the micron scale
    Julien E Gautrot
    Melville Laboratory for Polymer Synthesis, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK
    Biomaterials 31:5030-41. 2010
    ..This technology, combined with single cell analysis methods, provides a platform for exploring the mechanisms that regulate cell behaviour...
  75. pmc Autophagy mediates the mitotic senescence transition
    Andrew R J Young
    Cancer Research UK, Cambridge Research Institute, Li Ka Shing Centre, Cambridge, United Kingdom
    Genes Dev 23:798-803. 2009
    ..Furthermore, inhibition of autophagy delays the senescence phenotype, including senescence-associated secretion. Our data suggest that autophagy, and its consequent protein turnover, mediate the acquisition of the senescence phenotype...
  76. doi Nanog maintains pluripotency of mouse embryonic stem cells by inhibiting NFkappaB and cooperating with Stat3
    Josema Torres
    Wellcome Trust Centre for Stem Cell Research, Tennis Court Road, Cambridge CB2 1QR, UK
    Nat Cell Biol 10:194-201. 2008
    ..We conclude that Nanog represses the pro-differentiation activities of NFkappaB and cooperates with Stat3 to maintain pluripotency...
  77. doi Assaying proliferation and differentiation capacity of stem cells using disaggregated adult mouse epidermis
    Kim B Jensen
    Laboratory for Epidermal Stem Cell Biology, Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Cambridge, UK
    Nat Protoc 5:898-911. 2010
    ..Isolating, fractionating and grafting cells for the skin reconstitution assay is normally spread over 2 d. Clonal growth in culture is assessed after 14 d, while evaluation of the grafts is carried out after 4-5 weeks...
  78. pmc A tumor-associated beta 1 integrin mutation that abrogates epithelial differentiation control
    Richard D Evans
    Keratinocyte Laboratory, Cancer Research UK London Research Institute, London WC2A 3PX, UK
    J Cell Biol 160:589-96. 2003
    ..They also provide new insights into how integrins regulate keratinocyte differentiation...
  79. ncbi Expression of DeltaNLef1 in mouse epidermis results in differentiation of hair follicles into squamous epidermal cysts and formation of skin tumours
    Catherin Niemann
    Imperial Cancer Research Fund, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Development 129:95-109. 2002
    ....
  80. ncbi Sequence variation in the I-like domain of the beta1 integrin subunit in human oral squamous cell carcinomas
    Richard D Evans
    Keratinocyte Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, WC2A 3PX, UK
    Cancer Lett 213:189-94. 2004
    ..We conclude that mutations in the beta1 I-like domain are uncommon in SCCs. However, population based studies of the polymorphisms we found may reveal an association with SCC development or prognosis...
  81. ncbi Unexpected Hedgehog-Wnt interactions in epithelial differentiation
    Fiona M Watt
    Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Trends Mol Med 10:577-80. 2004
    ..The work is discussed in the broader context of Ihh expression and function in epithelia and the different ways in which the Hh and Wnt pathways interact...
  82. ncbi Human sebaceous tumors harbor inactivating mutations in LEF1
    Hikaru Takeda
    Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Nat Med 12:395-7. 2006
    ..Mutant LEF1 not only inhibited expression of beta-catenin target genes but also stimulated expression of sebocyte markers, suggesting that it may determine the differentiated characteristics of sebaceous tumors...
  83. pmc Stem cells are dispensable for lung homeostasis but restore airways after injury
    Adam Giangreco
    Cancer Research UK, Cambridge Research Institute, Cambridge, United Kingdom
    Proc Natl Acad Sci U S A 106:9286-91. 2009
    ..This study provides evidence that epithelial stem cells are dispensable for normal airway homeostasis. We also demonstrate that stem cell activation and robust clonal cellular expansion occur only during repair from severe lung injury...
  84. ncbi Epidermal label-retaining cells: background and recent applications
    Kristin M Braun
    Keratinocyte Laboratory, Cancer Research UK London Research Institute, London, UK
    J Investig Dermatol Symp Proc 9:196-201. 2004
    ..We describe how LRC can be visualized by whole-mount labelling of the epidermis and how purified LRC can be used to screen for markers of the epidermal stem cell compartment...
  85. ncbi Epidermal deletion of Rac1 causes stem cell depletion, irrespective of whether deletion occurs during embryogenesis or adulthood
    Salvador A Benitah
    J Invest Dermatol 127:1555-7. 2007
  86. ncbi A crucial role of beta 1 integrins for keratinocyte migration in vitro and during cutaneous wound repair
    Richard Grose
    Institute of Cell Biology, Department of Biology, ETH Zurich, 8093 Zurich, Switzerland
    Development 129:2303-15. 2002
    ..These findings demonstrate a crucial role of beta 1 integrins in keratinocyte migration and wound re-epithelialisation. Movies available on-line..
  87. doi Sic transit gloria: farewell to the epidermal transit amplifying cell?
    Philip H Jones
    MRC Cancer Cell Unit, Hutchison MRC Research Centre, Cambridge CB2 0XZ, UK
    Cell Stem Cell 1:371-81. 2007
    ..Recent studies challenge the existence of a transit amplifying cell compartment and suggest a new paradigm for epidermal homeostasis...
  88. pmc Myc regulates keratinocyte adhesion and differentiation via complex formation with Miz1
    Anneli Gebhardt
    Institute for Cell Biology, University of Marburg, 35033 Marburg, Germany
    J Cell Biol 172:139-49. 2006
    ..Our data show that regulation of cell adhesion is a major function of the Myc-Miz1 complex and suggest that it may contribute to Myc-induced exit from the epidermal stem cell compartment...
  89. ncbi Regulation of interleukin-1alpha expression by integrins and epidermal growth factor receptor in keratinocytes from a mouse model of inflammatory skin disease
    Robin M Hobbs
    Keratinocyte Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, United Kingdom
    J Biol Chem 278:19798-807. 2003
    ..These results provide a mechanism by which aberrant integrin expression triggers epidermal hyperproliferation and inflammation...
  90. ncbi Suprabasal alpha 5 beta1 integrin expression stimulates formation of epidermal squamous cell carcinomas without disrupting TGFbeta signaling or inducing spindle cell tumors
    David M Owens
    Department of Dermatology, Columbia University, College of Physicians and Surgeons, New York, NY, USA
    Mol Carcinog 44:60-6. 2005
    ....
  91. doi The vitamin D receptor is required for mouse hair cycle progression but not for maintenance of the epidermal stem cell compartment
    Hector G Palmer
    J Invest Dermatol 128:2113-7. 2008
  92. ncbi Keratinocyte differentiation is regulated by the Rho and ROCK signaling pathway
    Rachel McMullan
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom
    Curr Biol 13:2185-9. 2003
    ..Thus, these results indicate that ROCK plays a critical role in regulating the balance between proliferation and differentiation in human keratinocytes...
  93. ncbi The cell-surface marker MTS24 identifies a novel population of follicular keratinocytes with characteristics of progenitor cells
    Joanne G W Nijhof
    Department of Dermatology, Leiden University Medical Center LUMC The Netherlands
    Development 133:3027-37. 2006
    ..Taken together, these data suggest that the cell-surface marker MTS24 identifies a new reservoir of hair follicle keratinocytes with a proliferative capacity and gene expression profile suggestive of progenitor or stem cells...
  94. pmc Switch from alphavbeta5 to alphavbeta6 integrin expression protects squamous cell carcinomas from anoikis
    Sam M Janes
    Keratinocyte Laboratory, CR UK London Research Institute, 44 Lincoln s Inn Fields, WC2A 3PX, England
    J Cell Biol 166:419-31. 2004
    ..These results suggest that down-regulation of alphavbeta5 through up-regulation of alphavbeta6 may protect SCCs from anoikis by activating an Akt survival signal...
  95. doi Characterization of bipotential epidermal progenitors derived from human sebaceous gland: contrasting roles of c-Myc and beta-catenin
    Cristina Lo Celso
    Massachusetts General Hospital, Center for Regenerative Medicine, Boston, Massachusetts, USA
    Stem Cells 26:1241-52. 2008
    ..Our studies provide evidence for a bipotential epidermal stem cell population in an in vitro model of human epidermal lineage selection and highlight the importance of Myc as a regulator of sebocyte differentiation...
  96. ncbi Regulation of keratinocyte shape, migration and wound epithelialization by IGF-1- and EGF-dependent signalling pathways
    Ingo Haase
    Department of Dermatology and Center for Molecular Medicine, University of Cologne CMMC, Joseph Stelzmann Strasse 9, 50924 Cologne, Germany
    J Cell Sci 116:3227-38. 2003
    ..Our results show that IGF-1 and EGF can influence different components of the keratinocyte migration machinery that determines the speed of wound epithelialization...
  97. ncbi Amparo Cano
    Fiona M Watt
    J Cell Sci 117:3075-6. 2004