H D Ulrich

Summary

Affiliation: Cancer Research UK
Country: UK

Publications

  1. doi Regulating post-translational modifications of the eukaryotic replication clamp PCNA
    Helle D Ulrich
    Cancer Research UK London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, EN6 3LD, United Kingdom
    DNA Repair (Amst) 8:461-9. 2009
  2. ncbi Ubiquitin, SUMO, and phosphate: how a trio of posttranslational modifiers governs protein fate
    Helle D Ulrich
    Cancer Research UK London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms EN6 3LD, UK
    Mol Cell 47:335-7. 2012
  3. pmc Activation of ubiquitin-dependent DNA damage bypass is mediated by replication protein a
    Adelina A Davies
    Cancer Research UK London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms EN6 3LD, UK
    Mol Cell 29:625-36. 2008
  4. doi Ubiquitin signalling in DNA replication and repair
    Helle D Ulrich
    Cancer Research UK London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, Hertfordshire EN6 3LD, UK
    Nat Rev Mol Cell Biol 11:479-89. 2010
  5. doi The fast-growing business of SUMO chains
    Helle D Ulrich
    Clare Hall Laboratories, Cancer Research UK London Research Institute, Blanche Lane, South Mimms, EN6 3LD, UK
    Mol Cell 32:301-5. 2008
  6. doi Timing and spacing of ubiquitin-dependent DNA damage bypass
    Helle D Ulrich
    Cancer Research UK London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, Herts EN6 3LD, United Kingdom
    FEBS Lett 585:2861-7. 2011
  7. ncbi PCNASUMO and Srs2: a model SUMO substrate-effector pair
    H D Ulrich
    Cancer Research UK, Clare Hall Laboratories, London Research Institute, South Mimms EN6 3LD, U K
    Biochem Soc Trans 35:1385-8. 2007
  8. ncbi SUMO teams up with ubiquitin to manage hypoxia
    Helle D Ulrich
    Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, UK
    Cell 131:446-7. 2007
  9. ncbi Conservation of DNA damage tolerance pathways from yeast to humans
    H D Ulrich
    Cancer Research UK, London Research Institute, Clare Hall Laboratories, South Mimms EN6 3LD, U K
    Biochem Soc Trans 35:1334-7. 2007
  10. ncbi SUMO keeps a check on recombination during DNA replication
    Helle D Ulrich
    Cancer Research UK, Clare Hall Laboratories, South Mimms, United Kingdom
    Cell Cycle 4:1699-702. 2005

Collaborators

Detail Information

Publications36

  1. doi Regulating post-translational modifications of the eukaryotic replication clamp PCNA
    Helle D Ulrich
    Cancer Research UK London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, EN6 3LD, United Kingdom
    DNA Repair (Amst) 8:461-9. 2009
    ....
  2. ncbi Ubiquitin, SUMO, and phosphate: how a trio of posttranslational modifiers governs protein fate
    Helle D Ulrich
    Cancer Research UK London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms EN6 3LD, UK
    Mol Cell 47:335-7. 2012
    ..2012) demonstrate how a series of sequential posttranslational modifications, phosphorylation, sumoylation, and ubiquitylation, cooperate to target human flap endonuclease FEN1 to degradation by the proteasome at the end of S phase...
  3. pmc Activation of ubiquitin-dependent DNA damage bypass is mediated by replication protein a
    Adelina A Davies
    Cancer Research UK London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms EN6 3LD, UK
    Mol Cell 29:625-36. 2008
    ..Association of the ligase with chromatin is detected where RPA is most abundant, and purified RPA can recruit Rad18 to ssDNA in vitro. Our results therefore implicate the RPA complex in the activation of DNA damage tolerance...
  4. doi Ubiquitin signalling in DNA replication and repair
    Helle D Ulrich
    Cancer Research UK London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, Hertfordshire EN6 3LD, UK
    Nat Rev Mol Cell Biol 11:479-89. 2010
    ....
  5. doi The fast-growing business of SUMO chains
    Helle D Ulrich
    Clare Hall Laboratories, Cancer Research UK London Research Institute, Blanche Lane, South Mimms, EN6 3LD, UK
    Mol Cell 32:301-5. 2008
    ..Like ubiquitin itself, the small ubiquitin-related modifier SUMO can form polymeric chains on many of its targets. Recent analyses have provided evidence for a number of distinct biological functions of the poly-SUMO signal...
  6. doi Timing and spacing of ubiquitin-dependent DNA damage bypass
    Helle D Ulrich
    Cancer Research UK London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, Herts EN6 3LD, United Kingdom
    FEBS Lett 585:2861-7. 2011
    ..This review is focussed on our understanding of the timing of damage bypass during the cell cycle and the question of how it is coordinated with the progression of replication forks...
  7. ncbi PCNASUMO and Srs2: a model SUMO substrate-effector pair
    H D Ulrich
    Cancer Research UK, Clare Hall Laboratories, London Research Institute, South Mimms EN6 3LD, U K
    Biochem Soc Trans 35:1385-8. 2007
    ....
  8. ncbi SUMO teams up with ubiquitin to manage hypoxia
    Helle D Ulrich
    Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, UK
    Cell 131:446-7. 2007
    ..In this issue, Cheng et al. (2007) describe how the stability of the major transcriptional regulator of the hypoxic response, HIF1alpha, is modulated by the interplay between the ubiquitin and the SUMO conjugation systems...
  9. ncbi Conservation of DNA damage tolerance pathways from yeast to humans
    H D Ulrich
    Cancer Research UK, London Research Institute, Clare Hall Laboratories, South Mimms EN6 3LD, U K
    Biochem Soc Trans 35:1334-7. 2007
    ....
  10. ncbi SUMO keeps a check on recombination during DNA replication
    Helle D Ulrich
    Cancer Research UK, Clare Hall Laboratories, South Mimms, United Kingdom
    Cell Cycle 4:1699-702. 2005
    ..This review highlights how the SUMO conjugation system exerts its effect on the replication fork and discusses the implications for ubiquitin-dependent DNA damage tolerance...
  11. ncbi Mutual interactions between the SUMO and ubiquitin systems: a plea of no contest
    Helle D Ulrich
    Cancer Research UK, Clare Hall Laboratories, South Mimms, Herts, UK
    Trends Cell Biol 15:525-32. 2005
    ..This article gives an overview of target proteins that can serve as substrates for both SUMO and ubiquitin to highlight the diversity of regulatory strategies that result from the crosstalk between the two modification systems...
  12. ncbi The RAD6 pathway: control of DNA damage bypass and mutagenesis by ubiquitin and SUMO
    Helle D Ulrich
    Cancer Research UK, Clare Hall Laboratories, Blanche Lane, South Mimms, Hertfordshire, EN6 3LD, UK
    Chembiochem 6:1735-43. 2005
  13. ncbi SUMO modification: wrestling with protein conformation
    Helle D Ulrich
    Cancer Research UK, Clare Hall Laboratories, South Mimms, UK
    Curr Biol 15:R257-9. 2005
    ....
  14. pmc SUMO modification of PCNA is controlled by DNA
    Joanne L Parker
    Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, UK
    EMBO J 27:2422-31. 2008
    ..Instead, the stimulatory effect of DNA on conjugation is mainly attributable to DNA binding of PCNA itself. These findings imply a change in the properties of PCNA upon loading that enhances its capacity to be sumoylated...
  15. pmc Ubiquitin-dependent DNA damage bypass is separable from genome replication
    Yasukazu Daigaku
    Cancer Research UK London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms EN6 3LD, UK
    Nature 465:951-5. 2010
    ..Our approach has revealed the distribution of PRR tracts in a synchronized cell population. It will allow an in-depth mechanistic analysis of how cells manage the processing of lesions to their genomes during and after replication...
  16. pmc Contributions of ubiquitin- and PCNA-binding domains to the activity of Polymerase eta in Saccharomyces cerevisiae
    Joanne L Parker
    Cancer Research UK, London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, EN6 3LD, United Kingdom
    Nucleic Acids Res 35:881-9. 2007
    ..Like its mammalian homolog, budding yeast Polymerase eta itself is ubiquitylated in a manner dependent on its ubiquitin-binding domain...
  17. ncbi Cooperation of replication protein A with the ubiquitin ligase Rad18 in DNA damage bypass
    Diana Huttner
    Cancer Research UK, London Research Institute, Clare Hall Laboratories, South Mimms, Herts, UK
    Cell Cycle 7:3629-33. 2008
    ..Here we have examined in more detail the interactions between Rad18, RPA and DNA and discuss their contribution to the activation of DNA damage bypass...
  18. ncbi Crosstalk between SUMO and ubiquitin on PCNA is mediated by recruitment of the helicase Srs2p
    Efterpi Papouli
    Cancer Research UK, Clare Hall Laboratories, Blanche Lane, South Mimms, Herts EN6 3LD, United Kingdom
    Mol Cell 19:123-33. 2005
    ..Our findings suggest a mechanism by which SUMO and ubiquitin cooperatively control the choice of pathway for the processing of DNA lesions during replication...
  19. ncbi Architecture and assembly of poly-SUMO chains on PCNA in Saccharomyces cerevisiae
    Hanna Windecker
    Cancer Research UK London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms EN6 3LD, UK
    J Mol Biol 376:221-31. 2008
    ....
  20. pmc Mechanistic analysis of PCNA poly-ubiquitylation by the ubiquitin protein ligases Rad18 and Rad5
    Joanne L Parker
    Clare Hall Laboratories, Cancer Research UK London Research Institute, South Mimms, Herts, UK
    EMBO J 28:3657-66. 2009
    ..Our results provide information about a unique conjugation mechanism that appears to be specialised for a regulatable pattern of dual modification...
  21. doi The SUMO system: an overview
    Helle D Ulrich
    Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, UK
    Methods Mol Biol 497:3-16. 2009
    ..This chapter gives a brief overview over the enzymes of the SUMO system, its regulation, and its functions...
  22. pmc Distinct consequences of posttranslational modification by linear versus K63-linked polyubiquitin chains
    Shengkai Zhao
    Cancer Research UK London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms EN6 3LD, United Kingdom
    Proc Natl Acad Sci U S A 107:7704-9. 2010
    ..Our results suggest that the cellular machinery responsible for recognition of ubiquitylated substrates can make subtle distinctions between highly similar forms of the polyubiquitin signal...
  23. doi In vivo detection and characterization of sumoylation targets in Saccharomyces cerevisiae
    Helle D Ulrich
    Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, UK
    Methods Mol Biol 497:81-103. 2009
    ..Two well-studied model substrates, the septin Cdc3 and the replication clamp protein PCNA, are used as examples, but the protocol can easily be adapted to other targets and organisms...
  24. doi Ubiquitylation of the 9-1-1 checkpoint clamp is independent of rad6-rad18 and DNA damage
    Adelina A Davies
    Cancer Research UK London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms EN6 3LD, UK
    Cell 141:1080-7. 2010
    ..Instead, our findings suggest that ubiquitylation of the 9-1-1 complex may be a background reaction that in some cases can mediate proteasomal degradation...
  25. ncbi How to activate a damage-tolerant polymerase: consequences of PCNA modifications by ubiquitin and SUMO
    Helle D Ulrich
    Max Planck Institute for Terrestrial Microbiology, Karl von Frisch Strasse, Marburg, Germany
    Cell Cycle 3:15-8. 2004
  26. ncbi Control of spontaneous and damage-induced mutagenesis by SUMO and ubiquitin conjugation
    Philipp Stelter
    Max Planck Institute for Terrestrial Microbiology, Karl von Frisch Strasse, 35043 Marburg, Germany
    Nature 425:188-91. 2003
    ..Our findings assign a function to SUMO during S phase and demonstrate how ubiquitin and SUMO, by regulating the accuracy of replication and repair, contribute to overall genomic stability...
  27. ncbi Protein-protein interactions within an E2-RING finger complex. Implications for ubiquitin-dependent DNA damage repair
    Helle D Ulrich
    Max Planck Institute for Terrestrial Microbiology, Karl von Frisch Strasse, 35043 Marburg, Germany
    J Biol Chem 278:7051-8. 2003
    ..Finally, this study demonstrates that beyond its cooperation with the UBC13-MMS2 dimer, RAD5 must have an additional role in DNA damage repair independent of its RING finger domain...
  28. pmc Yeast MPH1 gene functions in an error-free DNA damage bypass pathway that requires genes from Homologous recombination, but not from postreplicative repair
    K Anke Schürer
    Department of Molecular Genetics and Preparative Molecular Biology, Institute for Microbiology and Genetics, University of Gottingen, D 37077 Gottingen, Germany
    Genetics 166:1673-86. 2004
    ..On the contrary, in an sgs1 background we found a pronounced hyperrecombination phenotype. Thus, we propose that MPH1 is involved in a branch of homologous recombination that is specifically dedicated to error-free bypass...
  29. pmc The RING finger ATPase Rad5p of Saccharomyces cerevisiae contributes to DNA double-strand break repair in a ubiquitin-independent manner
    Shuhua Chen
    Max Planck Institute for Terrestrial Microbiology, Karl von Frisch Strasse D 35043, Marburg, Germany
    Nucleic Acids Res 33:5878-86. 2005
    ....
  30. pmc Antagonistic roles of ESCRT and Vps class C/HOPS complexes in the recycling of yeast membrane proteins
    Amandine Bugnicourt
    Institut Jacques Monod Centre National de la Recherche Scientifique Universités Paris 6 and 7, 75251 Paris Cedex 05, France
    Mol Biol Cell 15:4203-14. 2004
    ..Genetic analyses indicated that these phenotypes were due to the functioning of the Vps class C complex in trafficking both to and from the late endosomal compartment...
  31. ncbi REV1 protein interacts with PCNA: significance of the REV1 BRCT domain in vitro and in vivo
    Caixia Guo
    Laboratory of Molecular Pathology, Department of Pathology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Mol Cell 23:265-71. 2006
    ..In vivo studies in both chicken DT40 cells and yeast directly support the requirement of the BRCT domain of REV1 for cell survival and DNA damage-induced mutagenesis...
  32. pmc Postreplication repair and PCNA modification in Schizosaccharomyces pombe
    Jonathan Frampton
    Genome Damage and Stability Centre, University of Sussex, Falmer, Brighton BN1 9RQ, United Kingdom
    Mol Biol Cell 17:2976-85. 2006
    ..We show that PCNA modification and cell cycle checkpoints represent two independent signals in response to DNA damage. Finally, we unexpectedly find that PCNA is ubiquitinated in response to DNA damage when cells are arrested in G2...
  33. ncbi Deubiquitinating PCNA: a downside to DNA damage tolerance
    Helle D Ulrich
    Nat Cell Biol 8:303-5. 2006
  34. pmc DNA interstrand crosslink repair during G1 involves nucleotide excision repair and DNA polymerase zeta
    Sovan Sarkar
    Cancer Research UK Laboratories, Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, UK
    EMBO J 25:1285-94. 2006
    ..We show that this combination of NER and TLS is the only pathway of ICL repair available to the cell in G1 phase and is essential for viability in the presence of DNA crosslinks...
  35. pmc Ubiquitin-binding motifs in REV1 protein are required for its role in the tolerance of DNA damage
    Caixia Guo
    Laboratory of Molecular Pathology, Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9072, USA
    Mol Cell Biol 26:8892-900. 2006
    ..In cells exposed to UV radiation, the association of REV1 with replication foci is dependent on functional UBMs. The UBMs of REV1 are shown to contribute to DNA damage tolerance and damage-induced mutagenesis in vivo...
  36. pmc Degradation or maintenance: actions of the ubiquitin system on eukaryotic chromatin
    Helle D Ulrich
    Max Planck Institute for Terrestrial Microbiology, D 35043 Marburg, Germany
    Eukaryot Cell 1:1-10. 2002