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Genomes and Genes | Frank UhlmannSummaryAffiliation: Cancer Research UK Country: UK Publications
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Publications
Cdk-counteracting phosphatases unlock mitotic exitEthel Queralt
Cancer Epigenetics and Biology Program PEBC, Catalan Institute of Oncology, Av Gran Via S N, 08907 L Hospitalet de Llobregat, Barcelona, Spain
Curr Opin Cell Biol 20:661-8. 2008..Much less is known about protein dephosphorylation during mitotic exit in organisms other than budding yeast, but evidence is now mounting for crucial contributions of regulated phosphatases also in metazoan cells...- Displacement and re-accumulation of centromeric cohesin during transient pre-anaphase centromere splittingMaria T Ocampo Hafalla
Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
Chromosoma 116:531-44. 2007..These findings provide new insights into cohesin behaviour at centromeres... - A matter of choice: the establishment of sister chromatid cohesionFrank Uhlmann
Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, London, UK
EMBO Rep 10:1095-102. 2009..I propose that the establishment of cohesion equates to the selective stabilization of those cohesin-mediated DNA interactions that link sister chromatids in the wake of replication forks... 
Chromosome cohesion and separation: from men and moleculesFrank Uhlmann
Lincoln s Inn Fields Laboratories, Cancer Research UK, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
Curr Biol 13:R104-14. 2003..Studies on separase have revealed new levels of regulation of chromosome segregation...- What is your assay for sister-chromatid cohesion?Frank Uhlmann
Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, London, UK
EMBO J 26:4609-18. 2007 - The mechanism of sister chromatid cohesionFrank Uhlmann
Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, London WC2A 3PX, UK
Exp Cell Res 296:80-5. 2004..To understand how cohesin links newly synthesised replication products, biochemical assays to study the enzymology of cohesin will be required... 
Hos1 deacetylates Smc3 to close the cohesin acetylation cycleVanessa Borges
Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, Lincoln s Inn Fields Laboratories, London WC2A 3PX, UK
Mol Cell 39:677-88. 2010..Our results complete the description of an Smc3 acetylation cycle and provide unexpected insight into the importance of de novo Smc3 acetylation for cohesion establishment...- Identification of cis-acting sites for condensin loading onto budding yeast chromosomesClaudio D'Ambrosio
Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, London, UK
Genes Dev 22:2215-27. 2008..This reveals that TFIIIC-binding sites, including tRNA genes, constitute a hitherto unknown chromosomal feature with important implications for chromosome architecture during both interphase and mitosis... - Establishment of sister chromatid cohesion at the S. cerevisiae replication forkArmelle Lengronne
Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, Lincoln s Inn Fields Laboratories, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
Mol Cell 23:787-99. 2006..This provides evidence for cohesion establishment in the context of replication forks and imposes constraints on the mechanism involved... - Eco1-dependent cohesin acetylation during establishment of sister chromatid cohesionTom Rolef Ben-Shahar
Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, 44 Lincoln sInn Fields, London WC2A 3PX, UK
Science 321:563-6. 2008..Our results indicate that Eco1 modifies cohesin to stabilize sister chromatid cohesion in parallel with a cohesion establishment reaction that is in principle Eco1-independent... - Stabilization of microtubule dynamics at anaphase onset promotes chromosome segregationToru Higuchi
Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, Lincoln's Inn Fields Laboratories, 44 Lincoln's Inn Fields, London WC2A 3PX, UK
Nature 433:171-6. 2005..Cdc14 promotes localization of microtubule-stabilizing proteins to the anaphase spindle, and dephosphorylation of the kinetochore component Ask1 contributes to both the silencing of microtubule turnover and successful anaphase A... - Cdc14 phosphatase induces rDNA condensation and resolves cohesin-independent cohesion during budding yeast anaphaseMatt Sullivan
Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, Lincoln's Inn Fields Laboratories, 44 Lincoln's Inn Fields, London WC2A 3PX, United Kingdom
Cell 117:471-82. 2004..Independently of condensation, Cdc14 induces condensin-dependent resolution of cohesin-independent rDNA linkage. Cdc14-dependent sister chromatid resolution at the rDNA could introduce a temporal order to chromosome segregation... - Studies on substrate recognition by the budding yeast separaseMatt Sullivan
Cancer Research UK London Research Institute, Lincoln s Inn Fields Laboratories, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
J Biol Chem 279:1191-6. 2004..This suggests that separase recognizes both a cleavage site consensus sequence as well as features outside the cleavage site... - Separase cooperates with Zds1 and Zds2 to activate Cdc14 phosphatase in early anaphaseEthel Queralt
Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, London, England, UK
J Cell Biol 182:873-83. 2008..Our results suggest that these proteins may act as separase-regulated PP2A(Cdc55) inhibitors... - Preferential cleavage of chromatin-bound cohesin after targeted phosphorylation by Polo-like kinaseNadine C D Hornig
Chromosome Segregation Laboratory, Cancer Research UK, London Research Institute, Lincoln s Inn Fields Laboratories, London, UK
EMBO J 23:3144-53. 2004..Polo is bound to chromosomes independently of cohesin's presence, providing a possible explanation for chromosome-specific cohesin modification and targeting of separase cleavage... - A quantitative model for cyclin-dependent kinase control of the cell cycle: revisitedFrank Uhlmann
Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3LY, UK
Philos Trans R Soc Lond B Biol Sci 366:3572-83. 2011..We propose a molecular explanation for quantitative Cdk control, based on thresholds imposed by Cdk-counteracting phosphatases, and discuss its implications... - Cohesin relocation from sites of chromosomal loading to places of convergent transcriptionArmelle Lengronne
Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, Lincoln s Inn Fields Laboratories, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
Nature 430:573-8. 2004..Cohesin localization to places of convergent transcription is conserved in fission yeast, suggesting that it is a common feature of eukaryotic chromosomes... - Downregulation of PP2A(Cdc55) phosphatase by separase initiates mitotic exit in budding yeastEthel Queralt
Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, Lincoln s Inn Fields Laboratories, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
Cell 125:719-32. 2006..These findings allow us to present a new quantitative model for mitotic exit in budding yeast... - Sli15(INCENP) dephosphorylation prevents mitotic checkpoint reengagement due to loss of tension at anaphase onsetLesia Mirchenko
Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London, UK
Curr Biol 20:1396-401. 2010..Our results suggest that Sli15(INCENP) dephosphorylation is part of an evolutionarily conserved mechanism that prevents the mitotic checkpoint from reengaging when tension between sister chromatids is lost at anaphase onset... - In vivo analysis of cohesin architecture using FRET in the budding yeast Saccharomyces cerevisiaeJohn Mc Intyre
Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, London, UK
EMBO J 26:3783-93. 2007..Using FRET, we did not observe interactions between more than one cohesin complex in vivo... - Condensin-dependent rDNA decatenation introduces a temporal pattern to chromosome segregationClaudio D'Ambrosio
Chromosome Segregation Laboratory, Cancer Research UK, London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
Curr Biol 18:1084-9. 2008..Regulation of decatenation therefore provides a means to fine tune the segregation timing of chromosomes in mitosis... - A model for ATP hydrolysis-dependent binding of cohesin to DNAStefan Weitzer
Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, Lincoln s Inn Fields Laboratories, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
Curr Biol 13:1930-40. 2003..The mechanism by which cohesin binds to DNA, and how sister chromatid cohesion is established, is unknown... - Chromosome biology: the crux of the ringFrank Uhlmann
Lincoln's Inn Fields Laboratories, Cancer Research UK London Research Institute, 44 Lincoln's Inn Fields, London WC2A 3PX, UK
Curr Biol 16:R102-5. 2006..New evidence supports the idea that these rings topologically encircle DNA. Hints also emerge as to what it may take for DNA to enter the ring... - Conserved features of cohesin binding along fission yeast chromosomesChristine K Schmidt
Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, Lincoln s Inn Fields, London WC2A 3PX, UK
Genome Biol 10:R52. 2009..How, and where, cohesin binds to chromosomes are still poorly understood, and recent genome-wide surveys have revealed an apparent disparity between its chromosomal association patterns in different organisms... - A quantitative model for ordered Cdk substrate dephosphorylation during mitotic exitCéline Bouchoux
Cancer Research UK London Research Institute, Lincoln s Inn Fields Laboratories, London WC2A 3LY, UK
Cell 147:803-14. 2011..This provides an example and a mechanistic explanation for a quantitative model of cell-cycle progression... - The 'anaphase problem': how to disable the mitotic checkpoint when sisters splitMaría Dolores Vázquez-Novelle
Cell Division and Aneuploidy Laboratory, Cancer Research UK, London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, Hertfordshire EN6 3LD, UK
Biochem Soc Trans 38:1660-6. 2010..Mitotic checkpoint inactivation at anaphase onset is required to prevent checkpoint re-engagement when sister chromatids split... - Cohesin loading and slidingMaria T Ocampo-Hafalla
Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, Lincoln s Inn Fields Laboratories, 44 Lincoln s Inn Fields, London WC2A 3LY, UK
J Cell Sci 124:685-91. 2011.... - A non-proteolytic function of separase links the onset of anaphase to mitotic exitMatt Sullivan
Chromosome Segregation Laboratory, Cancer Research UK, Lincoln s Inn Fields Laboratories, London, WC2A 3PX, UK
Nat Cell Biol 5:249-54. 2003.... - The dual mechanism of separase regulation by securinNadine C D Hornig
Chromosome Segregation Laboratory, WC2A 3PX, London, United Kingdom
Curr Biol 12:973-82. 2002..The molecular mechanisms underlying this dual regulation of separase by securin are unknown... - Irreversibility of mitotic exit is the consequence of systems-level feedbackSandra López-Avilés
Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
Nature 459:592-5. 2009..Our findings demonstrate that the unidirectionality of mitotic exit is not the consequence of proteolysis but of systems-level feedback required to maintain the cell cycle in a new stable state... - Cell cycle: the art of multi-taskingSandra López-Avilés
Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
Curr Biol 20:R101-3. 2010..This enzyme is known to orchestrate additional mitotic events and we now gain new insight into how it promotes cytokinesis in the nematode Caenorhabditis elegans... - Budding yeast Wapl controls sister chromatid cohesion maintenance and chromosome condensationLidia Lopez-Serra
Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3LY, UK
Curr Biol 23:64-9. 2013..Thus, as a conserved regulator of cohesin dynamics on chromosomes, Wapl controls cohesion maintenance after its establishment in S phase and adjusts the chromosome condensation status... - Facile synthesis of budding yeast a-factor and its use to synchronize cells of α mating typeNicola O'Reilly
Peptide Synthesis Laboratory, Cancer Research UK London Research Institute, UK
Yeast 29:233-40. 2012..Our protocol makes a-factor widely accessible, extending the ease of cell cycle synchronization to budding yeast cells of both mating types and facilitating the study of a-factor signalling... - An Eco1-independent sister chromatid cohesion establishment pathway in S. cerevisiaeVanessa Borges
Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London, WC2A 3LY, UK
Chromosoma 122:121-34. 2013..Thus, Ctf4 and Chl1 delineate an additional acetylation-independent pathway that might hold important clues as to the mechanism of sister chromatid cohesion establishment... - Condensin, chromatin crossbarring and chromosome condensationRahul Thadani
Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3LY, UK
Curr Biol 22:R1012-21. 2012..We compare models of bacterial and eukaryotic condensins in order to uncover conserved mechanistic principles of condensin action and to propose a model for mitotic chromosome condensation... - Chromosome segregation: playing polo in prophaseStefan Weitzer
Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, 44 Lincoln's Inn Fields, WC2A 3PX, London, United Kingdom
Dev Cell 2:381-2. 2002..A paper in the March issue of Molecular Cell identifies Polo-like kinase as a key regulator for the first step that releases much of cohesin during prophase... - Cell cycle: passenger acrobaticsToru Higuchi
Nature 426:780-1. 2003 - Division of the nucleolus and its release of CDC14 during anaphase of meiosis I depends on separase, SPO12, and SLK19Sara B C Buonomo
Research Institute of Molecular Pathology, Dr Bohr-Gasse 7, A-1030, Vienna, Austria
Dev Cell 4:727-39. 2003..It has two mechanistically different roles during meiosis I. Loss of the protease-independent function alone results in a second meiotic division occurring on anaphase I spindles in spo12delta and slk19delta mutants... - More than a separaseEthel Queralt
Nat Cell Biol 7:930-2. 2005 - Mitotic exit in two dimensionsAttila Toth
Molecular Network Dynamics Group of Hungarian Academy of Sciences and Budapest University of Technology and Economics, 1111 Budapest Gellert ter 4, Hungary
J Theor Biol 248:560-73. 2007.... - Cell-cycle regulation of cohesin stability along fission yeast chromosomesPascal Bernard
Universite Bordeaux 2, CNRS Institut de Biochimie et Génétique Cellulaires, Bordeaux, France
EMBO J 27:111-21. 2008..Hence, cohesin stabilization might be a pre-requisite for cohesion establishment rather than its consequence... - Molecular biology: cohesin branches outFrank Uhlmann
Nature 451:777-8. 2008 - Cell biology: keeping the genome in shapeFrank Uhlmann
Nature 417:135-6. 2002