Frank Uhlmann

Summary

Affiliation: Cancer Research UK
Country: UK

Publications

  1. ncbi request reprint Chromosome cohesion and separation: from men and molecules
    Frank Uhlmann
    Lincoln s Inn Fields Laboratories, Cancer Research UK, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Curr Biol 13:R104-14. 2003
  2. pmc Cdk-counteracting phosphatases unlock mitotic exit
    Ethel Queralt
    Cancer Epigenetics and Biology Program PEBC, Catalan Institute of Oncology, Av Gran Via S N, 08907 L Hospitalet de Llobregat, Barcelona, Spain
    Curr Opin Cell Biol 20:661-8. 2008
  3. pmc A matter of choice: the establishment of sister chromatid cohesion
    Frank Uhlmann
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, London, UK
    EMBO Rep 10:1095-102. 2009
  4. pmc What is your assay for sister-chromatid cohesion?
    Frank Uhlmann
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, London, UK
    EMBO J 26:4609-18. 2007
  5. ncbi request reprint The mechanism of sister chromatid cohesion
    Frank Uhlmann
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, London WC2A 3PX, UK
    Exp Cell Res 296:80-5. 2004
  6. doi request reprint Hos1 deacetylates Smc3 to close the cohesin acetylation cycle
    Vanessa Borges
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, Lincoln s Inn Fields Laboratories, London WC2A 3PX, UK
    Mol Cell 39:677-88. 2010
  7. pmc Identification of cis-acting sites for condensin loading onto budding yeast chromosomes
    Claudio D'Ambrosio
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, London, UK
    Genes Dev 22:2215-27. 2008
  8. doi request reprint Eco1-dependent cohesin acetylation during establishment of sister chromatid cohesion
    Tom Rolef Ben-Shahar
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, 44 Lincoln sInn Fields, London WC2A 3PX, UK
    Science 321:563-6. 2008
  9. ncbi request reprint Establishment of sister chromatid cohesion at the S. cerevisiae replication fork
    Armelle Lengronne
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, Lincoln s Inn Fields Laboratories, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
    Mol Cell 23:787-99. 2006
  10. pmc Stabilization of microtubule dynamics at anaphase onset promotes chromosome segregation
    Toru Higuchi
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, Lincoln s Inn Fields Laboratories, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Nature 433:171-6. 2005

Collaborators

  • Stefan Weitzer
  • Karl Peter Hopfner
  • Bela Novak
  • Yasuto Murayama
  • Iestyn Whitehouse
  • Duncan J Smith
  • Yoshinori Watanabe
  • Christine K Schmidt
  • Mark Petronczki
  • Kim Nasmyth
  • Ethel Queralt
  • Vanessa Borges
  • Matt Sullivan
  • Lidia Lopez-Serra
  • Katsuhiko Shirahige
  • Armelle Lengronne
  • Sandra López-Avilés
  • Maria T Ocampo-Hafalla
  • Claudio D'Ambrosio
  • Adrian Charbin
  • Toru Higuchi
  • Nadine C D Hornig
  • Céline Bouchoux
  • Yuki Katou
  • Lesia Mirchenko
  • Gavin Kelly
  • Tom Rolef Ben-Shahar
  • Attila Toth
  • Rahul Thadani
  • Nicola O'Reilly
  • Sebastian Heeger
  • María Dolores Vázquez-Novelle
  • Helen Flynn
  • Chris Lehane
  • Mark Skehel
  • Pascal Bernard
  • John Mc Intyre
  • Takehiko Itoh
  • Sara B C Buonomo
  • Orsolya Kapuy
  • Karl Ekwall
  • Philip East
  • Jean Paul Javerzat
  • Sonia Dheur
  • Sabine Vaur
  • Sylvie Genier
  • Julie Drogat
  • Brian E Snydsman
  • Eric G D Muller
  • Trisha N Davis
  • John McIntyre
  • Yutaka Kanoh
  • Thomas Porstmann
  • Gavin P Kelly
  • Vittorio L Katis
  • Shihori Yokobayashi
  • Saori Mori
  • Jörg Fuchs
  • Kirsten P Rabitsch
  • Stephan Gruber
  • Philip P Knowles
  • Neil Q McDonald

Detail Information

Publications45

  1. ncbi request reprint Chromosome cohesion and separation: from men and molecules
    Frank Uhlmann
    Lincoln s Inn Fields Laboratories, Cancer Research UK, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Curr Biol 13:R104-14. 2003
    ..Studies on separase have revealed new levels of regulation of chromosome segregation...
  2. pmc Cdk-counteracting phosphatases unlock mitotic exit
    Ethel Queralt
    Cancer Epigenetics and Biology Program PEBC, Catalan Institute of Oncology, Av Gran Via S N, 08907 L Hospitalet de Llobregat, Barcelona, Spain
    Curr Opin Cell Biol 20:661-8. 2008
    ..Much less is known about protein dephosphorylation during mitotic exit in organisms other than budding yeast, but evidence is now mounting for crucial contributions of regulated phosphatases also in metazoan cells...
  3. pmc A matter of choice: the establishment of sister chromatid cohesion
    Frank Uhlmann
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, London, UK
    EMBO Rep 10:1095-102. 2009
    ..I propose that the establishment of cohesion equates to the selective stabilization of those cohesin-mediated DNA interactions that link sister chromatids in the wake of replication forks...
  4. pmc What is your assay for sister-chromatid cohesion?
    Frank Uhlmann
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, London, UK
    EMBO J 26:4609-18. 2007
  5. ncbi request reprint The mechanism of sister chromatid cohesion
    Frank Uhlmann
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, London WC2A 3PX, UK
    Exp Cell Res 296:80-5. 2004
    ..To understand how cohesin links newly synthesised replication products, biochemical assays to study the enzymology of cohesin will be required...
  6. doi request reprint Hos1 deacetylates Smc3 to close the cohesin acetylation cycle
    Vanessa Borges
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, Lincoln s Inn Fields Laboratories, London WC2A 3PX, UK
    Mol Cell 39:677-88. 2010
    ..Our results complete the description of an Smc3 acetylation cycle and provide unexpected insight into the importance of de novo Smc3 acetylation for cohesion establishment...
  7. pmc Identification of cis-acting sites for condensin loading onto budding yeast chromosomes
    Claudio D'Ambrosio
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, London, UK
    Genes Dev 22:2215-27. 2008
    ..This reveals that TFIIIC-binding sites, including tRNA genes, constitute a hitherto unknown chromosomal feature with important implications for chromosome architecture during both interphase and mitosis...
  8. doi request reprint Eco1-dependent cohesin acetylation during establishment of sister chromatid cohesion
    Tom Rolef Ben-Shahar
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, 44 Lincoln sInn Fields, London WC2A 3PX, UK
    Science 321:563-6. 2008
    ..Our results indicate that Eco1 modifies cohesin to stabilize sister chromatid cohesion in parallel with a cohesion establishment reaction that is in principle Eco1-independent...
  9. ncbi request reprint Establishment of sister chromatid cohesion at the S. cerevisiae replication fork
    Armelle Lengronne
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, Lincoln s Inn Fields Laboratories, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
    Mol Cell 23:787-99. 2006
    ..This provides evidence for cohesion establishment in the context of replication forks and imposes constraints on the mechanism involved...
  10. pmc Stabilization of microtubule dynamics at anaphase onset promotes chromosome segregation
    Toru Higuchi
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, Lincoln s Inn Fields Laboratories, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Nature 433:171-6. 2005
    ..Cdc14 promotes localization of microtubule-stabilizing proteins to the anaphase spindle, and dephosphorylation of the kinetochore component Ask1 contributes to both the silencing of microtubule turnover and successful anaphase A...
  11. pmc An Eco1-independent sister chromatid cohesion establishment pathway in S. cerevisiae
    Vanessa Borges
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3LY, UK
    Chromosoma 122:121-34. 2013
    ..Thus, Ctf4 and Chl1 delineate an additional acetylation-independent pathway that might hold important clues as to the mechanism of sister chromatid cohesion establishment...
  12. doi request reprint Budding yeast Wapl controls sister chromatid cohesion maintenance and chromosome condensation
    Lidia Lopez-Serra
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3LY, UK
    Curr Biol 23:64-9. 2013
    ..Thus, as a conserved regulator of cohesin dynamics on chromosomes, Wapl controls cohesion maintenance after its establishment in S phase and adjusts the chromosome condensation status...
  13. pmc Separase cooperates with Zds1 and Zds2 to activate Cdc14 phosphatase in early anaphase
    Ethel Queralt
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, London, England, UK
    J Cell Biol 182:873-83. 2008
    ..Our results suggest that these proteins may act as separase-regulated PP2A(Cdc55) inhibitors...
  14. pmc Preferential cleavage of chromatin-bound cohesin after targeted phosphorylation by Polo-like kinase
    Nadine C D Hornig
    Chromosome Segregation Laboratory, Cancer Research UK, London Research Institute, Lincoln s Inn Fields Laboratories, London, UK
    EMBO J 23:3144-53. 2004
    ..Polo is bound to chromosomes independently of cohesin's presence, providing a possible explanation for chromosome-specific cohesin modification and targeting of separase cleavage...
  15. ncbi request reprint Studies on substrate recognition by the budding yeast separase
    Matt Sullivan
    Cancer Research UK London Research Institute, Lincoln s Inn Fields Laboratories, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
    J Biol Chem 279:1191-6. 2004
    ..This suggests that separase recognizes both a cleavage site consensus sequence as well as features outside the cleavage site...
  16. ncbi request reprint Cdc14 phosphatase induces rDNA condensation and resolves cohesin-independent cohesion during budding yeast anaphase
    Matt Sullivan
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, Lincoln s Inn Fields Laboratories, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
    Cell 117:471-82. 2004
    ..Independently of condensation, Cdc14 induces condensin-dependent resolution of cohesin-independent rDNA linkage. Cdc14-dependent sister chromatid resolution at the rDNA could introduce a temporal order to chromosome segregation...
  17. pmc A quantitative model for cyclin-dependent kinase control of the cell cycle: revisited
    Frank Uhlmann
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3LY, UK
    Philos Trans R Soc Lond B Biol Sci 366:3572-83. 2011
    ..We propose a molecular explanation for quantitative Cdk control, based on thresholds imposed by Cdk-counteracting phosphatases, and discuss its implications...
  18. ncbi request reprint Downregulation of PP2A(Cdc55) phosphatase by separase initiates mitotic exit in budding yeast
    Ethel Queralt
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, Lincoln s Inn Fields Laboratories, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
    Cell 125:719-32. 2006
    ..These findings allow us to present a new quantitative model for mitotic exit in budding yeast...
  19. pmc Cohesin relocation from sites of chromosomal loading to places of convergent transcription
    Armelle Lengronne
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, Lincoln s Inn Fields Laboratories, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Nature 430:573-8. 2004
    ..Cohesin localization to places of convergent transcription is conserved in fission yeast, suggesting that it is a common feature of eukaryotic chromosomes...
  20. ncbi request reprint A model for ATP hydrolysis-dependent binding of cohesin to DNA
    Stefan Weitzer
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, Lincoln s Inn Fields Laboratories, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
    Curr Biol 13:1930-40. 2003
    ..The mechanism by which cohesin binds to DNA, and how sister chromatid cohesion is established, is unknown...
  21. pmc Sli15(INCENP) dephosphorylation prevents mitotic checkpoint reengagement due to loss of tension at anaphase onset
    Lesia Mirchenko
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London, UK
    Curr Biol 20:1396-401. 2010
    ..Our results suggest that Sli15(INCENP) dephosphorylation is part of an evolutionarily conserved mechanism that prevents the mitotic checkpoint from reengaging when tension between sister chromatids is lost at anaphase onset...
  22. pmc In vivo analysis of cohesin architecture using FRET in the budding yeast Saccharomyces cerevisiae
    John Mc Intyre
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, London, UK
    EMBO J 26:3783-93. 2007
    ..Using FRET, we did not observe interactions between more than one cohesin complex in vivo...
  23. doi request reprint Condensin-dependent rDNA decatenation introduces a temporal pattern to chromosome segregation
    Claudio D'Ambrosio
    Chromosome Segregation Laboratory, Cancer Research UK, London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
    Curr Biol 18:1084-9. 2008
    ..Regulation of decatenation therefore provides a means to fine tune the segregation timing of chromosomes in mitosis...
  24. ncbi request reprint Chromosome biology: the crux of the ring
    Frank Uhlmann
    Lincoln s Inn Fields Laboratories, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Curr Biol 16:R102-5. 2006
    ..New evidence supports the idea that these rings topologically encircle DNA. Hints also emerge as to what it may take for DNA to enter the ring...
  25. pmc Displacement and re-accumulation of centromeric cohesin during transient pre-anaphase centromere splitting
    Maria T Ocampo-Hafalla
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Chromosoma 116:531-44. 2007
    ..These findings provide new insights into cohesin behaviour at centromeres...
  26. doi request reprint A quantitative model for ordered Cdk substrate dephosphorylation during mitotic exit
    Céline Bouchoux
    Cancer Research UK London Research Institute, Lincoln s Inn Fields Laboratories, London WC2A 3LY, UK
    Cell 147:803-14. 2011
    ..This provides an example and a mechanistic explanation for a quantitative model of cell-cycle progression...
  27. pmc Conserved features of cohesin binding along fission yeast chromosomes
    Christine K Schmidt
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, Lincoln s Inn Fields, London WC2A 3PX, UK
    Genome Biol 10:R52. 2009
    ..How, and where, cohesin binds to chromosomes are still poorly understood, and recent genome-wide surveys have revealed an apparent disparity between its chromosomal association patterns in different organisms...
  28. doi request reprint Cohesin loading and sliding
    Maria T Ocampo-Hafalla
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, Lincoln s Inn Fields Laboratories, 44 Lincoln s Inn Fields, London WC2A 3LY, UK
    J Cell Sci 124:685-91. 2011
    ....
  29. doi request reprint The 'anaphase problem': how to disable the mitotic checkpoint when sisters split
    María Dolores Vázquez-Novelle
    Cell Division and Aneuploidy Laboratory, Cancer Research UK, London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, Hertfordshire EN6 3LD, UK
    Biochem Soc Trans 38:1660-6. 2010
    ..Mitotic checkpoint inactivation at anaphase onset is required to prevent checkpoint re-engagement when sister chromatids split...
  30. pmc A non-proteolytic function of separase links the onset of anaphase to mitotic exit
    Matt Sullivan
    Chromosome Segregation Laboratory, Cancer Research UK, Lincoln s Inn Fields Laboratories, London, WC2A 3PX, UK
    Nat Cell Biol 5:249-54. 2003
    ....
  31. doi request reprint Condensin, chromatin crossbarring and chromosome condensation
    Rahul Thadani
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3LY, UK
    Curr Biol 22:R1012-21. 2012
    ..We compare models of bacterial and eukaryotic condensins in order to uncover conserved mechanistic principles of condensin action and to propose a model for mitotic chromosome condensation...
  32. pmc Irreversibility of mitotic exit is the consequence of systems-level feedback
    Sandra López-Avilés
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Nature 459:592-5. 2009
    ..Our findings demonstrate that the unidirectionality of mitotic exit is not the consequence of proteolysis but of systems-level feedback required to maintain the cell cycle in a new stable state...
  33. doi request reprint Cell cycle: the art of multi-tasking
    Sandra López-Avilés
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Curr Biol 20:R101-3. 2010
    ..This enzyme is known to orchestrate additional mitotic events and we now gain new insight into how it promotes cytokinesis in the nematode Caenorhabditis elegans...
  34. ncbi request reprint The dual mechanism of separase regulation by securin
    Nadine C D Hornig
    Chromosome Segregation Laboratory, WC2A 3PX, London, United Kingdom
    Curr Biol 12:973-82. 2002
    ..The molecular mechanisms underlying this dual regulation of separase by securin are unknown...
  35. pmc Chromosome segregation: how to open cohesin without cutting the ring?
    Yasuto Murayama
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, London, UK
    EMBO J 32:614-6. 2013
    ..Two new papers in The EMBO Journal address how chromatin can get into and out of the cohesin ring without requiring its cleavage...
  36. doi request reprint Facile synthesis of budding yeast a-factor and its use to synchronize cells of α mating type
    Nicola O'Reilly
    Peptide Synthesis Laboratory, Cancer Research UK London Research Institute, UK
    Yeast 29:233-40. 2012
    ..Our protocol makes a-factor widely accessible, extending the ease of cell cycle synchronization to budding yeast cells of both mating types and facilitating the study of a-factor signalling...
  37. pmc Condensin aids sister chromatid decatenation by topoisomerase II
    Adrian Charbin
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, London WC2A 3LY, UK
    Nucleic Acids Res 42:340-8. 2014
    ..Our results provide evidence that condensin prevents deleterious anaphase bridges during chromosome segregation by promoting sister chromatid decatenation. ..
  38. ncbi request reprint Chromosome segregation: playing polo in prophase
    Stefan Weitzer
    Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, WC2A 3PX, London, United Kingdom
    Dev Cell 2:381-2. 2002
    ..A paper in the March issue of Molecular Cell identifies Polo-like kinase as a key regulator for the first step that releases much of cohesin during prophase...
  39. ncbi request reprint Cell cycle: passenger acrobatics
    Toru Higuchi
    Nature 426:780-1. 2003
  40. ncbi request reprint Division of the nucleolus and its release of CDC14 during anaphase of meiosis I depends on separase, SPO12, and SLK19
    Sara B C Buonomo
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030, Vienna, Austria
    Dev Cell 4:727-39. 2003
    ..It has two mechanistically different roles during meiosis I. Loss of the protease-independent function alone results in a second meiotic division occurring on anaphase I spindles in spo12delta and slk19delta mutants...
  41. ncbi request reprint More than a separase
    Ethel Queralt
    Nat Cell Biol 7:930-2. 2005
  42. ncbi request reprint Mitotic exit in two dimensions
    Attila Toth
    Molecular Network Dynamics Group of Hungarian Academy of Sciences and Budapest University of Technology and Economics, 1111 Budapest Gellert ter 4, Hungary
    J Theor Biol 248:560-73. 2007
    ....
  43. doi request reprint Molecular biology: cohesin branches out
    Frank Uhlmann
    Nature 451:777-8. 2008
  44. pmc Cell-cycle regulation of cohesin stability along fission yeast chromosomes
    Pascal Bernard
    Universite Bordeaux 2, CNRS Institut de Biochimie et Génétique Cellulaires, Bordeaux, France
    EMBO J 27:111-21. 2008
    ..Hence, cohesin stabilization might be a pre-requisite for cohesion establishment rather than its consequence...
  45. ncbi request reprint Cell biology: keeping the genome in shape
    Frank Uhlmann
    Nature 417:135-6. 2002