Jesper Q Svejstrup

Summary

Affiliation: Cancer Research UK
Country: UK

Publications

  1. doi request reprint Synovial sarcoma mechanisms: a series of unfortunate events
    Jesper Q Svejstrup
    Cancer Research UK, London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, Hertfordshire EN6 3LD, UK
    Cell 153:11-2. 2013
  2. pmc Transcription: another mark in the tail
    Jesper Q Svejstrup
    Mechanisms of Transcription Laboratory, Cancer Research UK London Research Institute, South Mimms, UK
    EMBO J 31:2753-4. 2012
  3. ncbi request reprint Transcription. Histones face the FACT
    Jesper Q Svejstrup
    Cancer Research, London Research Institute, Clare Hall Laboratories, Hertfordshire, UK
    Science 301:1053-5. 2003
  4. ncbi request reprint Contending with transcriptional arrest during RNAPII transcript elongation
    Jesper Q Svejstrup
    Clare Hall Laboratories, Cancer Research UK London Research Institute, South Mimms, London EN6 3LD, UK
    Trends Biochem Sci 32:165-71. 2007
  5. ncbi request reprint The RNA polymerase II transcription cycle: cycling through chromatin
    Jesper Q Svejstrup
    Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire, EN6 3LD, UK
    Biochim Biophys Acta 1677:64-73. 2004
  6. ncbi request reprint Rescue of arrested RNA polymerase II complexes
    Jesper Q Svejstrup
    Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, UK
    J Cell Sci 116:447-51. 2003
  7. doi request reprint The interface between transcription and mechanisms maintaining genome integrity
    Jesper Q Svejstrup
    Clare Hall Laboratories, Cancer Research UK London Research Institute, Blanche Lane, South Mimms, EN6 3LD, UK
    Trends Biochem Sci 35:333-8. 2010
  8. ncbi request reprint Chromatin elongation factors
    Jesper Q Svejstrup
    Cancer Research UK, London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, UK
    Curr Opin Genet Dev 12:156-61. 2002
  9. pmc Distinct ubiquitin ligases act sequentially for RNA polymerase II polyubiquitylation
    Michelle Harreman
    Mechanisms of Transcription Laboratory, Clare Hall Laboratories, Cancer Research UK London Research Institute, Blanche Lane, South Mimms, Hertfordshire EN6 3LD, United Kingdom
    Proc Natl Acad Sci U S A 106:20705-10. 2009
  10. doi request reprint An iron-sulfur cluster domain in Elp3 important for the structural integrity of elongator
    Catherine Greenwood
    Clare Hall Laboratories, Cancer Research UK London Research Institute, Blanche Lane, South Mimms EN6 3LD, United Kingdom
    J Biol Chem 284:141-9. 2009

Collaborators

Detail Information

Publications55

  1. doi request reprint Synovial sarcoma mechanisms: a series of unfortunate events
    Jesper Q Svejstrup
    Cancer Research UK, London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, Hertfordshire EN6 3LD, UK
    Cell 153:11-2. 2013
    ..Kadoch and Crabtree now show that the resulting cellular transformation stems from disruption of the normal architecture and function of the human SWI/SNF (BAF) complex...
  2. pmc Transcription: another mark in the tail
    Jesper Q Svejstrup
    Mechanisms of Transcription Laboratory, Cancer Research UK London Research Institute, South Mimms, UK
    EMBO J 31:2753-4. 2012
    ..While important functions for Ser(2)-, Ser(5)-, and Ser(7)-phosphorylation have previously been described, a new report in The EMBO Journal now suggests an unexpectedly crucial role for Thr(4) phosphorylation as well...
  3. ncbi request reprint Transcription. Histones face the FACT
    Jesper Q Svejstrup
    Cancer Research, London Research Institute, Clare Hall Laboratories, Hertfordshire, UK
    Science 301:1053-5. 2003
  4. ncbi request reprint Contending with transcriptional arrest during RNAPII transcript elongation
    Jesper Q Svejstrup
    Clare Hall Laboratories, Cancer Research UK London Research Institute, South Mimms, London EN6 3LD, UK
    Trends Biochem Sci 32:165-71. 2007
    ..In particular, studies of RNAPII ubiquitylation and degradation have provided new insight into how cells handle obstacle-induced transcriptional arrest...
  5. ncbi request reprint The RNA polymerase II transcription cycle: cycling through chromatin
    Jesper Q Svejstrup
    Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire, EN6 3LD, UK
    Biochim Biophys Acta 1677:64-73. 2004
    ..This review gives a brief overview of the transcription cycle as a whole, focusing especially on selected mechanisms that may drive or restrict the cycle, and on how the presence of chromatin may influence these mechanisms...
  6. ncbi request reprint Rescue of arrested RNA polymerase II complexes
    Jesper Q Svejstrup
    Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, UK
    J Cell Sci 116:447-51. 2003
    ..However, when this action is not possible and Pol II is left irreversibly trapped on DNA, the polymerase is instead ubiquitylated and eventually removed by proteolysis...
  7. doi request reprint The interface between transcription and mechanisms maintaining genome integrity
    Jesper Q Svejstrup
    Clare Hall Laboratories, Cancer Research UK London Research Institute, Blanche Lane, South Mimms, EN6 3LD, UK
    Trends Biochem Sci 35:333-8. 2010
    ..This review provides an overview of our present knowledge of how eukaryotic RNA polymerase II transcription affects, and is affected by, other DNA-related processes such as chromatin remodeling, DNA repair, recombination and replication...
  8. ncbi request reprint Chromatin elongation factors
    Jesper Q Svejstrup
    Cancer Research UK, London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, UK
    Curr Opin Genet Dev 12:156-61. 2002
    ..Insights into the function of these factors have been gained by a combination of yeast genetics and biochemical studies in mammalian systems...
  9. pmc Distinct ubiquitin ligases act sequentially for RNA polymerase II polyubiquitylation
    Michelle Harreman
    Mechanisms of Transcription Laboratory, Clare Hall Laboratories, Cancer Research UK London Research Institute, Blanche Lane, South Mimms, Hertfordshire EN6 3LD, United Kingdom
    Proc Natl Acad Sci U S A 106:20705-10. 2009
    ....
  10. doi request reprint An iron-sulfur cluster domain in Elp3 important for the structural integrity of elongator
    Catherine Greenwood
    Clare Hall Laboratories, Cancer Research UK London Research Institute, Blanche Lane, South Mimms EN6 3LD, United Kingdom
    J Biol Chem 284:141-9. 2009
    ..Together our data support the idea that the Elp3 FeS cluster is essential for normal Elongator function in vivo primarily as a structural, rather than catalytic, domain...
  11. ncbi request reprint Multiple mechanisms confining RNA polymerase II ubiquitylation to polymerases undergoing transcriptional arrest
    Baggavalli P Somesh
    Cancer Research UK London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, Herts EN6 3LD, United Kingdom
    Cell 121:913-23. 2005
    ..These results identify several mechanisms that confine ubiquitylation of RNAPII to the forms of the enzyme that arrest during elongation...
  12. pmc An rtt109-independent role for vps75 in transcription-associated nucleosome dynamics
    Luke A Selth
    Clare Hall Laboratories, Cancer Research UK London Research Institute, Blanche Lane, South Mimms, Hertfordshire, U K
    Mol Cell Biol 29:4220-34. 2009
    ..These results indicate a role for Vps75 in nucleosome dynamics during transcription, and importantly, this function appears to be largely independent of Rtt109...
  13. pmc Proteasome-mediated processing of Def1, a critical step in the cellular response to transcription stress
    Marcus D Wilson
    Mechanisms of Transcription Laboratory, Clare Hall Laboratories, Cancer Research UK London Research Institute, South Mimms EN6 3LD, UK
    Cell 154:983-95. 2013
    ..Together, these results outline the multistep mechanism of Rpb1 polyubiquitylation triggered by transcription stress and uncover the key role played by Def1 as a facilitator of Elongin-Cullin ubiquitin ligase function. ..
  14. ncbi request reprint Physical and functional interaction between Elongator and the chromatin-associated Kti12 protein
    Thodoris G Petrakis
    Cancer Research UK London Research Institute, Clare Hall Laboratories, Hertfordshire, UK
    J Biol Chem 280:19454-60. 2005
    ..Together, these experiments indicate a close physical and functional relationship between Elongator and the highly conserved Kti12 protein...
  15. pmc GTP-dependent binding and nuclear transport of RNA polymerase II by Npa3 protein
    Lidija Staresincic
    Mechanisms of Transcription Laboratory, Clare Hall Laboratories, Cancer Research UK London Research Institute, South Mimms EN6 3LD, UK
    J Biol Chem 286:35553-61. 2011
    ..Together, our data suggest that Npa3 defines an unconventional pathway for nuclear import of RNAPII, which involves GTP-dependent binding of Npa3 to the polymerase...
  16. pmc A ubiquitin-binding domain in Cockayne syndrome B required for transcription-coupled nucleotide excision repair
    Roy Anindya
    Clare Hall Laboratories, Cancer Research UK London Research Institute, Blanche Lane, South Mimms EN6 3LD, UK
    Mol Cell 38:637-48. 2010
    ..Together, our results indicate an essential role for protein ubiquitylation and CSB's UBD in triggering damage incision during TC-NER and allow us to integrate the function of CSA and CSB in a model for the process...
  17. doi request reprint Reversal of RNA polymerase II ubiquitylation by the ubiquitin protease Ubp3
    Kristian Kvint
    Cancer Research UK London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, Herts EN6 3LD, UK
    Mol Cell 30:498-506. 2008
    ..In agreement with this, cells with compromised DNA repair are better equipped to survive UV damage when UPB3 is deleted...
  18. ncbi request reprint Communication between distant sites in RNA polymerase II through ubiquitylation factors and the polymerase CTD
    Baggavalli P Somesh
    Mechanisms of Transcription Laboratory, Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, UK
    Cell 129:57-68. 2007
    ..These data reveal the specificity and mechanism of RNAPII ubiquitylation and demonstrate that E2s can play a crucial role in substrate recognition...
  19. ncbi request reprint Molecular architecture, structure-function relationship, and importance of the Elp3 subunit for the RNA binding of holo-elongator
    Thodoris G Petrakis
    Cancer Research UK, London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, Hertfordshire EN6 3LD, United Kingdom
    J Biol Chem 279:32087-92. 2004
    ..In contrast, Elongator-RNA interaction requires the Elp3 protein. Together, these data shed light on the structure-function relationship of the Elongator complex...
  20. pmc Evidence for distinct mechanisms facilitating transcript elongation through chromatin in vivo
    Arnold Kristjuhan
    Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, UK
    EMBO J 23:4243-52. 2004
    ....
  21. ncbi request reprint DNA damage-induced Def1-RNA polymerase II interaction and Def1 requirement for polymerase ubiquitylation in vitro
    James Reid
    Cancer Research UK London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, Hertfordshire EN6 3LD, United Kingdom
    J Biol Chem 279:29875-8. 2004
    ..These results support a model in which Def1 interacts with RNAPII in response to DNA damage, recruiting the ubiquitylation machinery to enable its modification and subsequent degradation...
  22. pmc Spreading of Sir3 protein in cells with severe histone H3 hypoacetylation
    Arnold Kristjuhan
    Mechanisms of Transcription Laboratory, Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, United Kingdom
    Proc Natl Acad Sci U S A 100:7551-6. 2003
    ..Interestingly, growth defects caused by gcn5 elp3 mutation can be suppressed by SIR deletion, suggesting that Sir proteins become detrimental for growth when chromatin is severely hypoacetylated...
  23. pmc Evidence that transcript cleavage is essential for RNA polymerase II transcription and cell viability
    Stefan Sigurdsson
    Clare Hall Laboratories, Cancer Research UK London Research Institute, South Mimms, UK
    Mol Cell 38:202-10. 2010
    ..Our results suggest that transcription problems leading to backtracking are frequent in vivo and that reactivation of backtracked RNAPII is crucial for transcription...
  24. ncbi request reprint Interaction of Fcp1 phosphatase with elongating RNA polymerase II holoenzyme, enzymatic mechanism of action, and genetic interaction with elongator
    Stephanie E Kong
    Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, EN6 3LD, United Kingdom
    J Biol Chem 280:4299-306. 2005
    ..Together, these results shed new light on mechanisms that drive the transcription cycle and point to a role for Fcp1 in the recycling of RNAPII after dissociation from active genes...
  25. ncbi request reprint Hyperphosphorylation of the C-terminal repeat domain of RNA polymerase II facilitates dissociation of its complex with mediator
    T Max M Søgaard
    Clare Hall Laboratories, Cancer Research UK London Research Institute, Blanche Lane, South Mimms, UK
    J Biol Chem 282:14113-20. 2007
    ..These data shed new light on mechanisms that drive the RNAPII transcription cycle and suggest a mechanism for the enhancement of CTD kinase activity by the Mediator complex...
  26. ncbi request reprint Transcriptional inhibition of genes with severe histone h3 hypoacetylation in the coding region
    Arnold Kristjuhan
    Mechanisms of Transcription Laboratory, Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, EN6 3LD, Hertfordshire, United Kingdom
    Mol Cell 10:925-33. 2002
    ..These data provide insights into the effects of histone H3 hypoacetylation in vivo and underscore the importance of the overall charge of the histone tail for transcription...
  27. pmc Elongator is a histone H3 and H4 acetyltransferase important for normal histone acetylation levels in vivo
    G Sebastiaan Winkler
    Cancer Research UK, London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, United Kingdom
    Proc Natl Acad Sci U S A 99:3517-22. 2002
    ..By using chromatin immunoprecipitation, we show that the levels of multiply acetylated histone H3 and H4 in chromatin are decreased in vivo in yeast cells lacking ELP3...
  28. pmc A role for checkpoint kinase-dependent Rad26 phosphorylation in transcription-coupled DNA repair in Saccharomyces cerevisiae
    Michael Taschner
    Clare Hall Laboratories, Cancer Research UK London Research Institute, Blanche Lane, South Mimms, Hertfordshire EN6 3LD, United Kingdom
    Mol Cell Biol 30:436-46. 2010
    ..These findings establish a direct role for Mec1 kinase in transcription-coupled repair, at least partly via phosphorylation of Rad26, the main transcription-repair coupling factor...
  29. ncbi request reprint Damage-induced ubiquitylation of human RNA polymerase II by the ubiquitin ligase Nedd4, but not Cockayne syndrome proteins or BRCA1
    Roy Anindya
    Clare Hall Laboratories, Cancer Research UK London Research Institute, Blanche Lane, South Mimms EN6 3LD, UK
    Mol Cell 28:386-97. 2007
    ..Nedd4-dependent RNAPII ubiquitylation could also be reconstituted with highly purified proteins. Together, our results indicate that transcriptional arrest at DNA lesions triggers Nedd4 recruitment and RNAPII ubiquitylation...
  30. ncbi request reprint Isolation and mass spectrometry of transcription factor complexes
    G Sebastiaan Winkler
    Cancer Research UK London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, EN6 3LD, Hertfordshire, UK
    Methods 26:260-9. 2002
    ..As an example, we provide the procedures used to isolate and analyze yeast Elongator, a histone acetyltransferase complex important for transcript elongation, which led to the identification of three novel subunits...
  31. ncbi request reprint A Rad26-Def1 complex coordinates repair and RNA pol II proteolysis in response to DNA damage
    Elies C Woudstra
    Mechanisms of Gene Transcription Laboratory, Cancer Research UK, Clare Hall Laboratories, Blanche Lane, South Mimms, Hertfordshire EN6 3LD, UK
    Nature 415:929-33. 2002
    ....
  32. ncbi request reprint Elongator interactions with nascent mRNA revealed by RNA immunoprecipitation
    Christopher Gilbert
    Cancer Research UK London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, Hertfordshire EN6 3LD, United Kingdom
    Mol Cell 14:457-64. 2004
    ..These results strongly support a role for Elongator in transcript elongation...
  33. ncbi request reprint Incision of a 1,3-intrastrand d(GpTpG)-cisplatin adduct by nucleotide excision repair proteins from yeast
    Stephanie E Kong
    Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire, UK
    DNA Repair (Amst) 1:731-41. 2002
    ....
  34. pmc Stability, flexibility, and dynamic interactions of colliding RNA polymerase II elongation complexes
    Hideaki Saeki
    Mechanisms of Transcription Laboratory, Clare Hall Laboratories, Cancer Research UK London Research Institute, Blanche Lane, South Mimms, Hertfordshire EN6 3LD, UK
    Mol Cell 35:191-205. 2009
    ....
  35. pmc Role of elongator subunit Elp3 in Drosophila melanogaster larval development and immunity
    Jane Walker
    Clare Hall Laboratories, Cancer Research UK London Research Institute, South Mimms EN6 3LD, United Kingdom
    Genetics 187:1067-75. 2011
    ..Together, these data demonstrate that Drosophila Elp3 is essential for viability, normal development, and hematopoiesis and suggest a functional overlap with the chromatin remodeler Domino...
  36. pmc RNA polymerase II collision interrupts convergent transcription
    David J Hobson
    Mechanisms of Transcription Laboratory, Clare Hall Laboratories, Cancer Research UK London Research Institute, South Mimms, London EN6 3LD, UK
    Mol Cell 48:365-74. 2012
    ..These results provide insight into fundamental mechanisms of gene traffic control and point to an unexplored effect of antisense transcription on gene regulation via polymerase collision...
  37. ncbi request reprint Mechanisms of transcription-coupled DNA repair
    Jesper Q Svejstrup
    Imperial Cancer Research Fund, Clare Hall Laboratories, Blanche Lane, South Mimms, Hertfordshire EN6 3LD, UK
    Nat Rev Mol Cell Biol 3:21-9. 2002
    ..In this review, our knowledge of eukaryotic transcription-coupled repair (TCR) will be considered from the point of view of transcription, and current models for the mechanism of TCR will be discussed...
  38. doi request reprint Transcript Elongation by RNA Polymerase II
    Luke A Selth
    Mechanisms of Transcription Laboratory, Clare Hall Laboratories, Cancer Research UK London Research Institute, South Mimms, Hertfordshire EN6 3LD, United Kingdom
    Annu Rev Biochem 79:271-93. 2010
    ..This review addresses basic aspects of transcript elongation by RNA polymerase II (RNAPII) and how it relates to other DNA-related processes...
  39. pmc Direct inhibition of RNA polymerase II transcription by RECQL5
    Ozan Aygün
    Mechanisms of Transcription Laboratory, Clare Hall Laboratories, Cancer Research UK, London Research Institute, Blanche Lane, South Mimms, Hertfordshire EN6 3LD, United Kingdom
    J Biol Chem 284:23197-203. 2009
    ..Indeed, RECQL5 helicase activity is not required for inhibition. We discuss our findings in light of the fact that RECQ5(-/-) mice have elevated levels of DNA recombination and a higher incidence of cancer...
  40. ncbi request reprint Elongator complex: how many roles does it play?
    Jesper Q Svejstrup
    Clare Hall Laboratories, Cancer Research UK London Research Institute, Blanche Lane, South Mimms, EN6 3LD, UK
    Curr Opin Cell Biol 19:331-6. 2007
    ..One of the subunits of Elongator is encoded by a gene that is mutated in patients suffering from the severe neurodevelopmental disorder familial dysautonomia...
  41. doi request reprint Ubiquitylation and degradation of elongating RNA polymerase II: the last resort
    Marcus D Wilson
    Mechanisms of Transcription Laboratory, Cancer Research UK London Research Institute, South Mimms, UK
    Biochim Biophys Acta 1829:151-7. 2013
    ..In this review, we describe the mechanisms and factors responsible for the last resort mechanism of transcriptional elongation. This article is part of a Special Issue entitled: RNA polymerase II Transcript Elongation...
  42. ncbi request reprint Transcription repair coupling factor: a very pushy enzyme
    Jesper Q Svejstrup
    Cancer Research UK London Research Institute, Clare Hall Laboratory, South Mimms, EN6 3LD, UK
    Mol Cell 9:1151-2. 2002
    ..A recent study of the bacterial Mfd protein (Park et al., 2002 [Cell volume 109]) provides new insight into mechanisms that release RNAP from sites of arrest such as noncoding DNA lesions...
  43. ncbi request reprint Vps75, a new yeast member of the NAP histone chaperone family
    Luke Selth
    Clare Hall Laboratories, Cancer Research UK London Research Institute, Blanche Lane, South Mimms, EN6 3LD, United Kingdom
    J Biol Chem 282:12358-62. 2007
    ....
  44. pmc DBIRD complex integrates alternative mRNA splicing with RNA polymerase II transcript elongation
    Pierre Close
    Mechanisms of Transcription Laboratory, Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms EN6 3LD, UK
    Nature 484:386-9. 2012
    ..Together, these data indicate that the DBIRD complex acts at the interface between mRNP particles and RNAPII, integrating transcript elongation with the regulation of alternative splicing...
  45. pmc A role for noncoding transcription in activation of the yeast PHO5 gene
    Jay P Uhler
    Mechanisms of Transcription Laboratory, Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms EN6 3LD, United Kingdom
    Proc Natl Acad Sci U S A 104:8011-6. 2007
    ..We propose that noncoding transcription through positioned nucleosomes can enhance chromatin plasticity so that chromatin remodeling and activation of traversed genes occur in a timely manner...
  46. doi request reprint RNA immunoprecipitation for determining RNA-protein associations in vivo
    Chris Gilbert
    Cancer Research UK, London Research Institute, Clare Hall Laboratories, Hertfordshire, UK
    Curr Protoc Mol Biol . 2006
    ..This unit describes the RIP procedure for Saccharomyces cerevisiae. Although the corresponding steps for metazoan cells have not yet been worked out, it is likely that the yeast procedure can easily be adapted for use in other organisms...
  47. ncbi request reprint An assay for studying ubiquitylation of RNA polymerase II and other proteins in crude yeast extracts
    James Reid
    Cancer Research United Kingdom, London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire
    Methods Enzymol 408:264-73. 2006
    ..These procedures, combined with the ease of genetic manipulations in yeast, provide a new powerful tool for investigating the mechanisms underlying protein ubiquitylation...
  48. doi request reprint RECQL5 helicase: connections to DNA recombination and RNA polymerase II transcription
    Ozan Aygün
    Mechanisms of Transcription Laboratory, Clare Hall Laboratories, Cancer Research UK London Research Institute, Blanche Lane, South Mimms, Hertfordshire EN6 3LD, United Kingdom
    DNA Repair (Amst) 9:345-53. 2010
    ..Here, we describe the possible implications of recent studies and discuss models for RECQL5 function...
  49. pmc MultiDsk: a ubiquitin-specific affinity resin
    Marcus D Wilson
    Mechanisms of Transcription Laboratory, Clare Hall Laboratories, Cancer Research UK London Research Institute, Potters Bar, United Kingdom
    PLoS ONE 7:e46398. 2012
    ..We use the resin to show that the Def1 protein becomes ubiquitylated in response to DNA damage, and to isolate ubiquitylated forms of RNA polymerase II...
  50. ncbi request reprint RNA polymerase II: a "nobel" enzyme demystified
    Jesper Q Svejstrup
    Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, EN6 3LD, United Kingdom
    Mol Cell 24:637-42. 2006
  51. ncbi request reprint Purification and characterization of the human elongator complex
    Nicola A Hawkes
    Mechanisms of Gene Transcription Laboratory, Imperial Cancer Research Fund Clare Hall Laboratories, Blanche Lane, South Mimms, Hertfordshire, EN6 3LD, United Kingdom
    J Biol Chem 277:3047-52. 2002
    ..Mutations in IKAP can result in the severe human disorder familial dysautonomia, raising the possibility that this disease might be due to compromised Elongator function and therefore could be a transcription disorder...
  52. ncbi request reprint Purification of elongating RNA polymerase II and other factors from yeast chromatin
    Jesper Q Svejstrup
    London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, United Kingdom
    Methods Enzymol 371:491-8. 2003
  53. ncbi request reprint RNA Immunoprecipitation to Determine RNA-Protein Associations In Vivo
    Luke A Selth
    Mechanisms of Gene Transcription Laboratory, Clare Hall Laboratories, Cancer Research UK London Research Institute, South Mimms, Hertfordshire EN6 3LD, UK
    CSH Protoc 2009:pdb.prot5234. 2009
    ..The basics of RIP are very similar to chromatin immunoprecipitation (ChIP), but with some caveats that are important to appreciate to take full advantage of the possibilities afforded by RIP...
  54. pmc The NC2 alpha and beta subunits play different roles in vivo
    Sandrine Creton
    Department Biochimie Medicale, CMU, Geneva 4, Switzerland
    Genes Dev 16:3265-76. 2002
    ..Thus, the separation of NC2 alpha and beta function indicated by these mutants also supports the existence of multiple NC2 complexes with different functions in transcription...
  55. ncbi request reprint Transcription impairment and cell migration defects in elongator-depleted cells: implication for familial dysautonomia
    Pierre Close
    Laboratory of Medical Chemistry and Human Genetics, Center for Biomedical Integrative Genoproteomics, University of Liege, Belgium
    Mol Cell 22:521-31. 2006
    ..These results indicate that defects in Elongator function affect transcriptional elongation of several genes and that the ensuing cell motility deficiencies may underlie the neuropathology of FD patients...