Barbara Sedgwick

Summary

Affiliation: Cancer Research UK
Country: UK

Publications

  1. ncbi Oxidative demethylation by Escherichia coli AlkB directly reverts DNA base damage
    Sarah C Trewick
    Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, UK
    Nature 419:174-8. 2002
  2. ncbi Recent progress on the Ada response for inducible repair of DNA alkylation damage
    Barbara Sedgwick
    Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, UK
    Oncogene 21:8886-94. 2002
  3. ncbi Repair of alkylated DNA: recent advances
    Barbara Sedgwick
    Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, UK
    DNA Repair (Amst) 6:429-42. 2007
  4. ncbi Repairing DNA-methylation damage
    Barbara Sedgwick
    Cancer Research UK London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, Hertfordshire EN6 3LD, UK
    Nat Rev Mol Cell Biol 5:148-57. 2004
  5. ncbi Demethylation of 3-methylthymine in DNA by bacterial and human DNA dioxygenases
    Pertti Koivisto
    Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, United Kingdom
    J Biol Chem 279:40470-4. 2004
  6. pmc Reversal of DNA alkylation damage by two human dioxygenases
    Tod Duncan
    Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, United Kingdom
    Proc Natl Acad Sci U S A 99:16660-5. 2002
  7. ncbi Minimal methylated substrate and extended substrate range of Escherichia coli AlkB protein, a 1-methyladenine-DNA dioxygenase
    Pertti Koivisto
    Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, United Kingdom
    J Biol Chem 278:44348-54. 2003
  8. ncbi Direct removal of alkylation damage from DNA by AlkB and related DNA dioxygenases
    Barbara Sedgwick
    London Research Institute, Cancer Research United Kingdom, Clare Hall Laboratories, South Mimms, Hertfordshire
    Methods Enzymol 408:108-20. 2006
  9. ncbi Sensitization of human carcinoma cells to alkylating agents by small interfering RNA suppression of 3-alkyladenine-DNA glycosylase
    Johanna Paik
    Clare Hall Laboratories, Cancer Research UK London Research Institute, South Mimms, Hertfordshire, United Kingdom
    Cancer Res 65:10472-7. 2005
  10. ncbi Replacement of non-heme Fe(II) with Cu(II) in the alpha-ketoglutarate dependent DNA repair enzyme AlkB: spectroscopic characterization of the active site
    Boris Bleijlevens
    Division of Molecular Biosciences, Faculty of Natural Sciences, Imperial College London, South Kensington Campus, Biochemistry Building, London SW7 2AZ, UK
    J Inorg Biochem 101:1043-8. 2007

Collaborators

  • Tomas Lindahl
  • K S Hewitson
  • F M Ashcroft
  • Michael A McDonough
  • ROBERT HAUSINGER
  • Pertti Koivisto
  • Tod Duncan
  • Tara Shivarattan
  • Sarah C Trewick
  • Boris Bleijlevens
  • Thomas Gerken
  • Peter Robins
  • Johanna Paik
  • Christophe A Girard
  • Zorica Jovanovic
  • Patrik Rorsman
  • Luke A McNeill
  • Christopher J Schofield
  • Steve J Matthews
  • Stephen E J Rigby
  • Ines Barroso
  • Vladimir Saudek
  • Anthony P Coll
  • Xavier Prieur
  • Chris P Ponting
  • Yi Chun Loraine Tung
  • I Sadaf Farooqi
  • Sharon Cunliffe
  • Giles S H Yeo
  • Celia J Webby
  • Juris Galvanovskis
  • Stephen O'Rahilly
  • Marcella Ma
  • Pete Simpson
  • Ho Ann Chen
  • Stephen Matthews
  • Paul A Bates
  • Timothy F Henshaw

Detail Information

Publications12

  1. ncbi Oxidative demethylation by Escherichia coli AlkB directly reverts DNA base damage
    Sarah C Trewick
    Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, UK
    Nature 419:174-8. 2002
    ..The AlkB enzyme couples oxidative decarboxylation of alpha-ketoglutarate to the hydroxylation of these methylated bases in DNA, resulting in direct reversion to the unmodified base and the release of formaldehyde...
  2. ncbi Recent progress on the Ada response for inducible repair of DNA alkylation damage
    Barbara Sedgwick
    Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, UK
    Oncogene 21:8886-94. 2002
  3. ncbi Repair of alkylated DNA: recent advances
    Barbara Sedgwick
    Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, UK
    DNA Repair (Amst) 6:429-42. 2007
    ..We provide a more detailed description of the structures and biochemical properties of the recently discovered DNA dioxygenases...
  4. ncbi Repairing DNA-methylation damage
    Barbara Sedgwick
    Cancer Research UK London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, Hertfordshire EN6 3LD, UK
    Nat Rev Mol Cell Biol 5:148-57. 2004
    ..They use iron-oxo intermediates to oxidize stable methylated bases in DNA and directly revert them to the unmodified form...
  5. ncbi Demethylation of 3-methylthymine in DNA by bacterial and human DNA dioxygenases
    Pertti Koivisto
    Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, United Kingdom
    J Biol Chem 279:40470-4. 2004
    ..Our data suggest that 3-methylthymine residues in DNA will be repaired inefficiently in vivo and therefore may occur at a low steady-state level, but the residues should not gradually accumulate to high levels in long lived cells...
  6. pmc Reversal of DNA alkylation damage by two human dioxygenases
    Tod Duncan
    Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, United Kingdom
    Proc Natl Acad Sci U S A 99:16660-5. 2002
    ..AlkB, ABH2, and ABH3 can also repair 1-ethyladenine residues in DNA with the release of acetaldehyde...
  7. ncbi Minimal methylated substrate and extended substrate range of Escherichia coli AlkB protein, a 1-methyladenine-DNA dioxygenase
    Pertti Koivisto
    Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, United Kingdom
    J Biol Chem 278:44348-54. 2003
    ..AlkB is known to repair methyl and ethyl adducts in DNA; to extend this substrate range, AlkB was shown to reduce the toxic effects of DNA damaging agents that generate hydroxyethyl, propyl, and hydroxypropyl adducts...
  8. ncbi Direct removal of alkylation damage from DNA by AlkB and related DNA dioxygenases
    Barbara Sedgwick
    London Research Institute, Cancer Research United Kingdom, Clare Hall Laboratories, South Mimms, Hertfordshire
    Methods Enzymol 408:108-20. 2006
    ..Functionality in vivo is examined by complementation of the low survival of alkylated single-stranded DNA bacteriophage in an E. coli alkB mutant...
  9. ncbi Sensitization of human carcinoma cells to alkylating agents by small interfering RNA suppression of 3-alkyladenine-DNA glycosylase
    Johanna Paik
    Clare Hall Laboratories, Cancer Research UK London Research Institute, South Mimms, Hertfordshire, United Kingdom
    Cancer Res 65:10472-7. 2005
    ..Our data support the hypothesis that ablation of AAG activity in human tumor cells may provide a useful strategy to enhance the efficacy of current chemotherapeutic regimens that include alkylating agents...
  10. ncbi Replacement of non-heme Fe(II) with Cu(II) in the alpha-ketoglutarate dependent DNA repair enzyme AlkB: spectroscopic characterization of the active site
    Boris Bleijlevens
    Division of Molecular Biosciences, Faculty of Natural Sciences, Imperial College London, South Kensington Campus, Biochemistry Building, London SW7 2AZ, UK
    J Inorg Biochem 101:1043-8. 2007
    ..This work provides a basis for advanced EPR approaches aimed at studying the interactions and dynamics of AlkB complexes in solution...
  11. ncbi Resonance assignments of Escherichia coli AlkB: a key 2-oxoglutarate and Fe(II) dependent dioxygenase of the adaptive DNA-repair response
    Tara Shivarattan
    J Biomol NMR 33:138. 2005
  12. pmc The obesity-associated FTO gene encodes a 2-oxoglutarate-dependent nucleic acid demethylase
    Thomas Gerken
    Chemistry Research Laboratory and Oxford Centre for Integrative Systems Biology, University of Oxford, 12 Mansfield Road, Oxford, Oxon OX1 3TA, UK
    Science 318:1469-72. 2007
    ..Studies can now be directed toward determining the physiologically relevant FTO substrate and how nucleic acid methylation status is linked to increased fat mass...